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Nicotiana benthamiana XYLEM CYSTEINE PROTEASE genes facilitate tracheary element formation in interfamily grafting

Horticulture Research 2023年第6期10卷 70-81页

作者： Chaokun Huang ken-ichi kurotani Ryo Tabata Nobutaka Mitsuda Ryohei Sugita Keitaro Tanoi Michitaka Notaguchi Graduate School of Bioagricultural Sciences Nagoya UniversityFuro-choChikusa-kuNagoya 464-8601Japan Bioscience and Biotechnology Center Nagoya UniversityFuro-choChikusa-kuNagoya 464-8601Japan Bioproduction Research Institute National Institute of Advanced Industrial Science and Technology(AIST)TsukubaIbaraki 305-8566Japan Isotope Facility for Agricultural Education and Research Graduate School of Agricultural and Life SciencesThe University of TokyoYayoiBunkyo-kuTokyo 113-8657Japan Radioisotope Research Center Nagoya UniversityFuro-choChikusa-kuNagoya 464-8602Japan Institute of Transformative Bio-Molecules Nagoya UniversityFuro-choChikusa-kuNagoya 464-8601Japan

Grafting is a plant propagation technique widely used in agriculture.A recent discovery of the capability of interfamily grafting in Nicotiana has expanded the potential combinations of *** this study,we showed that xylem connection is essential for the achievement of interfamily grafting and investigated the molecular basis of xylem formation at the graft *** and gene network analyses revealed gene modules for tracheary element(TE)formation during grafting that include genes associated with xylem cell differentiation and immune *** reliability of the drawn network was validated by examining the role of the Nicotiana benthamiana XYLEM CYSTEINE PROTEASE(NbXCP)genes in TE formation during interfamily *** activities of NbXCP1 and NbXCP2 genes were found in differentiating TE cells in the stem and callus tissues at the graft *** of a Nbxcp1;Nbxcp2 loss-of-function mutant indicated that NbXCPs control the timing of de novo TE formation at the graft ***,grafts of the NbXCP1 overexpressor increased the scion growth rate as well as the fruit ***,we identified gene modules for TE formation at the graft boundary and demonstrated potential ways to enhance Nicotiana interfamily grafting.

关键词： grafting element graft

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Microfluidic Device for Simple Diagnosis of Plant Growth Condition by Detecting miRNAs from Filtered Plant Extracts

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Plant Phenomics 2024年第2期6卷 364-376页

作者： Yaichi Kawakatsu Ryo Okada Mitsuo Hara Hiroki Tsutsui Naoki Yanagisawa Tetsuya Higashiyama Akihide Arima Yoshinobu Baba ken-ichi kurotani Michitaka Notaguchi Bioscience and Biotechnology Center Nagoya UniversityFuro-choChikusa-kuNagoya 464-8601Japan Graduate School of Bioagricultural Sciences Nagoya UniversityFuro-choChikusa-kuNagoya 464-8601Japan Department of Molecular and Macromolecular Chemistry Graduate School of EngineeringNagoya UniversityFuro-choChikusa-kuNagoyaAichi 464-8603Japan Graduate School of Science Nagoya UniversityFuro-choChikusa-kuNagoya 464-8601Japan Institute of Transformative Bio-Molecules Nagoya UniversityNagoya 464-8601Japan Department of Biological Sciences Graduate School of ScienceThe University of Tokyo7-3-1 HongoBunkyo-kuTokyo 113-0033Japan Institute of Nano-Life-Systems Institutes of Innovation for Future SocietyNagoya UniversityFuro-choChikusa-kuNagoya 464-8603Japan Department of Biomolecular Engineering Graduate School of EngineeringNagoya UniversityFuro-choChikusa-kuNagoya 464-8603Japan Institute of Quantum Life Science National Institutes for Quantum Science and Technology(QST)Anagawa 4-9-1Inage-kuChiba 263-8555Japan Department of Botany Graduate School of ScienceKyoto UniversityKitashirakawa Oiwake-choSakyo-kuKyoto 606-8502Japan

Plants are exposed to a variety of environmental stress,and starvation of inorganic phosphorus can be a major constraint in crop *** plants,in response to phosphate deficiency in soil,miR399,a type of microRNA(miRNA),is *** detecting miR399,the early diagnosis of phosphorus deficiency stress in plants can be ***,general miRNA detection methods require complicated experimental ***,simple and rapid miRNA detection methods are required for early plant nutritional diagnosis.

关键词： mirnas plant device simple growth from condition detecting diagnosis extracts

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New aspects of a small GTPase RAB35 in brain development and function

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Neural Regeneration Research 2025年第7期20卷 1971-1980页

作者： Ikuko Maejima ken Sato Laboratory of Molecular Traffic Institute for Molecular and Cellular RegulationGunma UniversityMaebashiJapan

In eukaryotic cells,organelles in the secretory,lysosomal,and endocytic pathways actively exchange biological materials with each other through intracellular membrane trafficking,which is the process of transporting the cargo of proteins,lipids,and other molecules to appropriate compartments via transport vesicles or *** processes are strictly regulated by various small GTPases such as the RAS-like in rat brain(RAB)protein family,which is the largest subfamily of the RAS *** of membrane trafficking affects tissue homeostasis and leads to a wide range of diseases,including neurological disorders and neurodegenerative ***,it is important to understand the physiological and pathological roles of RAB proteins in brain ***35,a member of the RAB family,is an evolutionarily conserved protein in metazoans.A wide range of studies using cultured mammalian cells and model organisms have revealed that RAB35 mediates various processes such as cytokinesis,endocytic recycling,actin bundling,and cell ***35 is also involved in neurite outgrowth and turnover of synaptic *** generated brain-specific Rab35 knockout mice to study the physiological roles of RAB35 in brain development and *** mice exhibited defects in anxiety-related behaviors and spatial ***,RAB35 is required for the precise positioning of pyramidal neurons during hippocampal development,and thereby for normal hippocampal *** contrast,layer formation in the cerebral cortex occurred superficially,even in the absence of RAB35,suggesting a predominant role for RAB35 in hippocampal development rather than in cerebral cortex *** studies have suggested an association between RAB35 and neurodegenerative diseases,including Parkinson's disease and Alzheimer's *** this review,we provide an overview of the current understanding of subcellular functions of *** also provide insights into the phys

关键词： endocytosis endosomes hippocampal development neurodegenerative diseases RAB35

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Exploring the novel role of oligodendrocyte precursor cells in phagocytosis:beyond myelinogenesis

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Neural Regeneration Research 2025年第2期20卷 473-474页

作者： Gen Hamanaka ken Arai Neuroprotection Research Laboratories Departments of Radiology and NeurologyMassachusetts General Hospital and Harvard Medical SchoolCharlestownMAUSA

Roles of oligodendrocyte precursor cells in the central nervous system:Oligodendrocyte precursor cells(OPCs)have long been recognized for their critical role as precursors to oligodendrocytes,the primary myelin-producing *** precursors,OPCs mature and differentiate into oligodendrocytes,which contribute significantly to the formation of myelin sheaths around *** myelination,which is critical for the conduction of salutatory nerve impulses in the cerebral white matter,underscores the classical role of oligodendrocytes in central nervous system(CNS)***,because oligodendrocytes are differentiated cells that cannot proliferate.

关键词： critical system conduction

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Microwave Behavior of α-Fe/Fe_3B/Y_2O_3 Nanocomposites in GHz Range Prepared by Melt-Spun Technique

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Journal of Rare Earths 2004年第1期22卷 13-16页

作者： Masahiro Itoh ken-ichi Machida Collaborative Research Center for Advanced Science and Technology Collaborative Research Center for Advanced Science and Technology Osaka University 2-1 Yamadaoka Suita Osaka 565-0871 JapanOsaka University 2-1 Yamadaoka Suita Osaka 565-0871 Japan

Nanocomposites α-Fe/Fe 3B/Y 2O 3 were prepared by a melt-spun technique, and the electromagnetic wave absorption properties were measured in the 0 05～20 05 GHz range. Compared with α-Fe/Y 2O 3 composites, the resonance frequency (f r) of α-Fe/Fe 3B/Y 2O 3 shifted to a higher frequency range due to the large anisotropy field (H A) of tetragonal Fe 3B (～0 4 mA·m -1). The relative permittivity (ε r=ε r′-jε r″) was constantly low over the 0 5～10 GHz region, which indicates that the composite powders have a high resistivity (ρ=～100 Ωm). The effective electromagnetic wave absorption (RL<-20 dB) was obtained in a frequency range of 2.7～6.5 GHz on resin composites of 80% (mass fraction) α-Fe/Fe 3B/Y 2O 3 powders, with thickness of 6～3 mm respectively. A minimum reflection loss of -33 dB was observed at 4.5 GHz with an absorber thickness of 4 mm.

关键词： melt-spun nanocomposites electromagnetic wave absorption anisotropy field matching frequency

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Effects of Freestream Turbulence on Bypass Transition of Separated Boundary Layer on Low-Pressure Turbine Airfoils

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Journal of Thermal Science 2012年第3期21卷 230-235页

作者： Hideo Taniguchi Hiroshi Sakai ken-ichi Funazaki Department of Mechanical Systems Engineering Faculty of EngineeringIwate University

This paper presents experimental studies on bypass transition of separated boundary layer on low-pressure turbine airfoils,focusing on the effects of freestream turbulence on the transition ***-wire probe measurements are performed on the suction side of an airfoil in the low-pressure linear turbine cascade at several Reynolds number *** turbulence is enhanced by use of turbulence grid located upstream of the *** results of this experimental study show that the location of boundary layer separation does not strongly de-pend on the freestream turbulence ***,as the freestream turbulence level increases,the size of separa-tion bubble becomes small and the location of turbulent transition moves *** size of separation bubble becomes small as the Reynolds number *** low freestream turbulence intensity,the velocity fluctuation due to Kelvin-Helmholtz instability is observed clearly in the shear layer of the separation *** high frees-tream turbulence intensity,the streak structures appear upstream of the separation location,indicating bypass transition of attached boundary layer occurs at high Reynolds number.

关键词： Aeroengine Low-Pressure Turbine Bypass Transition Separation Bubble

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Influence of Heat Treatment on Hydroformability of TRIP Seamless Steel Tube

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Journal of Iron and Steel Research International 2014年第7期21卷 695-701页

作者： Zi-cheng ZHANG ken-ichi MANABE School of Mechanical Engineering and Automation Northeastern University Department of Mechanical Engineering Tokyo Metropolitan University

A low-carbon TRIP seamless steel tube, which is expected to be used in the hydroforming process, was successfully fabricated using piercing, cold-drawing and two-stage heat treatment process. The two-stage heat treatment is one crucial step because it significantly affects the microstructure and mechanical properties of TRIP seam less steel tube. In order to obtain the TRIP seamless steel tube with high hydroformability, several different heat treatment processes were conducted. The effects of heat treatment conditions （intercritical annealing （IA） and isothermal bainite treatment （IBT）） on the TRIP seamless steel tube hydroformability which was determined by free hydraulic bulge test were analyzed. Two different internal pressure boosting velocities of 0.2 and 0.5 MPa/s of free hydraulic bulge tests were adopted to determine the effective stress vs. effective strain curve of TRIP seamless steel tube. The results showed that for the predetermined IA condition, the maximum bulge height increased, but the maximum burst internal pressure decreased, with the increase of IBT holding time from 4 to 6 rain. For the predetermined IBT condition, the maximum bulge height decreased, but the maximum burst internal pressure increased, with the increase of IA holding time from 5 to 10 rain. By analyzing the free hydraulic bulge test results, it was found that the maximum bulge heights of TRIP seamless steel tubes with the internal pressure boosting velocity of 0.5 MPa/s were higher than those when the internal pressure boosting velocity was 0.2 MPa/s. This means that an appropriate deformation rate should be chosen to obtain the optimal hydroformability of TRIP seamless steel tube. In addition, the effective stress vs. effective strain curves of TRIP seamless steel tubes were ohtained with free hydraulic bulge test.

关键词： TRIP steel heat treatment hydroformability seamless tube hydraulic bulge test

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Numerical and Experimental Studies of an Arc-heated Nonequilibrium Nozzle Flow

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Journal of Thermal Science 2003年第4期12卷 289-293页

作者： Michio Nishida ken-ichi Abe Hisashi Kihara Department of Aeronautics and Astronautics Kyushu University Fukuoka 812-8581 JapanDepartment of Aeronautics and Astronautics Kyushu University Fukuoka 812-8581 JapanDepartment of Aeronautics and Astronautics Kyushu University Fukuoka 812-8581 Japan

The arc-heated high-temperature gas is rotationally and vibrationally excited, and partially dissociated and ionized. When such gas flows inside a nozzle, energy transfers from rotational and vibrational energy modes to translational energy mode, and, in addition, recombination reactions occur. These processes are in thermal and chemical nonequilibrium. The present computations treat arc-heated nonequilibrium nozzle flows using a six temperature model (translational, rotational, N2 vibrational, O2 vibrational, NO vibrational and electron temperatures), and nonequilibrium chemical reactions of air. From the calculated flow properties, emission spectra at the nozzle exit were re-constructed by using the code for computing spectra of high temperature air. On the other hand, measurements of N2+(1-) emission spectra were conducted at the nozzle exit in the 20 kW arc-heated wind tunnel. Vibrational and rotational temperatures of N2 were determined using a curve fitting method on N2+(1-) emission spectra, with the vibrational and rotational temperatures for N2 and N2+ being assumed equal. Comparison of the measured and computed results elucidated that the experimental temperatures were larger than the computed ones. At present, we are trying to reveal the main reason for the discrepancy between the computed and measured N2 vibrational and rotational temperatures.

关键词： nozzle flow high temperature gas nonequilibrium flow arc-heated gas.

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Synthesis and luminescent properties of low oxygen contained Eu^(2+)-doped Ca-α-SiAlON phosphor from calcium cyanamide reduction

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Journal of Rare Earths 2008年第2期26卷 198-202页

作者： PIAO Xianqing ken-ichi Machida Takashi Horikawa Hiromasa Hanzawa Center for Advanced Science and Innovation Osaka University 2-1 Yamadaoka Suita

A new convenient calcium cyanamide （CaCN2） reduction route was developed to synthesize the Eu^2＋ activated Ca-α-SiAION phosphors containing low oxygen content. The luminescence properties of the obtained products were investigated for white LEDs application. The critical Eu^2＋ concentration in various hosts and its effect on the photoluminescence properties were studied. The optimized sample （10at.% Eu^2＋ vs. Ca^2＋） could be efficiently excited by the current GaN/InGaN blue LED chips and provided emission intensity competitive with that of YAG：Ce^3＋（P46-Y3） standard, revealing that this phosphor was a potential candidate for phosphor-converted white LEDs.

关键词： nitride phosphor white LEDs SiAION Eu^2+ CaCN2 rare earths

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Recycle for Sludge Scrap of Nd-Fe-B Sintered Magnet as Isotropic Bonded Magnet

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Journal of Rare Earths 2004年第1期22卷 168-171页

作者： Masahiro Itoh Masahiro Masuda Shunji Suzuki ken-ichi Machida Collaborative Research Center for Advanced Science and Technology Collaborative Research Center for Advanced Science and TechnologyCollaborative Research Center for Advanced Science and TechnologyCollaborative Research Center for Advanced Science and Technology Osaka University 2-1 Yamadaoka Suita Osaka 565-0871 Osaka University 2-1 Yamadaoka Suita Osaka 565-0871 Osaka University 2-1 Yamadaoka Suita Osaka 565-0871 Osaka University 2-1 Yamadaoka Suita Osaka 565-0871

The reduction diffusion method was performed for the sludge scrap of Nd-Fe-B sintered magnets with adding Ca metal to recover the oxidized Nd-Fe-B phase. After washing the resultant powders to remove Ca metal component, the powders obtained were recycled as an isotropic magnetic powder by the melt spinning method. The magnetic properties of powders as recycled were inferior, especially for the coercivity value, due to the deletion of rare earth metals during the washing process. The adjustment of metal composition, i.e., the addition of Nd metal, at the melt spinning process improved the magnetic properties to be B r=～0.75 T, H cj=～0.93 mA·m -1, and (BH) max=～91 kJ·m -3. The magnetic properties of the bonded magnets prepared from the composition-adjusted powders were B r=～0.66 T, H cj=～0.92 mA·m -1, and (BH) max=～70 kJ·m -3, which are approximately comparable to the commercially available MQPB boned one (B r=～0.73 T, H cj=～0.79 mA·m -1, and (BH) max=～86 kJ·m -3).

关键词： Nd-Fe-B sintered magnet recycling isotropic Nd-Fe-B bonded magnet melt-spinning method sludge scrap rare earths

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