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Extracellular Vesicles from Mesenchymal Stromal Cells (imEVs) Improve Cold Preservation of Isolated Mitochondria

Journal of Biosciences and Medicines 2024年第1期12卷 52-63页

作者： Xianpeng Jiang Sergey Rodin ken braesch-andersen Catherine C. Baucom Karl-Henrik Grinnemo Brent Segal Elliott Mitochondrial Center Sallie Astor Burdine Breast Foundation Baton Rouge Louisiana USA Mitosense Inc. Boston Massachusetts USA Department of Surgical Sciences Division of Cardiothoracic Surgery Cardio-Thoracic Translational Medicine (CTTM) Laboratory Uppsala University Uppsala University Hospital Uppsala Sweden

Mitochondrial organelle transplantation (MOT) is an innovative strategy for the treatment of mitochondrial dysfunction such as cardiac ischemic reperfusion injuries, Parkinson’s diseases, brain and spinal cord injuries, and amyotrophic lateral sclerosis (ALS). However, one of the major challenges for widespread usage is a methodology for preservation of isolated mitochondria. Extracellular vesicles (EVs) are phospholipid bilayer-enclosed vesicles released from cells. EVs carry a cargo of proteins, nucleic acids, lipids, metabolites, and even organelles such as mitochondria. Purpose: To test if EVs enhance the stability of isolated mitochondria. Methods: We mixed isolated mitochondria of fibroblasts with EVs of mesenchymal stromal cells (imEVs) (9:1 in volume) and stored the mixture at 2°C - 6°C for different time periods. We measured morphology, mitochondrial membrane potential (MMP) and mitochondrial ATP content at 0, 2, 5 days. Key findings: After 2 days of storage, the mito-chondria without imEVs lost approximate 70% MMP (RFU: 1822 ± 68), compared to the fresh mitochondria (RFU: 5458 ± 52) (p 0.05). In agreement with MMP, mitochondria without imEVs lost significant mitochondrial ATP content (p 0.05), after 2 days of cold storage, compared to fresh mitochondria. Microscopy showed that imEVs promoted aggregation of isolated mitochondria. Summary: The preliminary data showed that imEVs enhanced the stability of isolated mitochondria in cold storage.

关键词： Mitochondria Extracellular Vesicles Mitochondrial Preservation MOT imEVs

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Antimicrobial activity of PVP from an Antarctic bacterium, Janthinobacterium sp. Ant5-2, on multi-drug and methicillin resistant Staphylococcus aureus

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Natural Products and Bioprospecting 2012年第3期2卷 104-110页

作者： Jonathan P.HUANG Nazia MOJIB Rakesh R.GOLI Samantha WATKINS ken B.WAITES Rasik RAVINDRA Dale T.andersen Asim K.BEJ Department of Biology University of Alabama at BirminghamBirminghamAL 35294-1170USA Department of Pathology University of Alabama at BirminghamBirminghamAL 35294-1170USA National Centre for Antarctic&Ocean Research Head Land SadaVasco-da-Gama Goa 403804India Carl Sagan Center for the Study of Life in the Universe SETI InstituteMountain ViewCA 94043USA Red Sea Research Center King Abdullah University of Science and TechnologyThuwal 23955-6900Saudi Arabia

Multiple drug resistant(MDR)and methicillin-resistant Staphylococcus aureus(MRSA)have become increasingly prevalent as a community acquired *** a result limited treatment options are available with conventional synthetic *** natural products with potent antimicrobial activity show promise for developing new drugs against this *** this study,we have investigated the antimicrobial activity of a purple violet pigment(PVP)from an Antarctic bacterium,Janthinobacterium ***5-2 on 15 clinical MDR and MRSA *** colorimetric resazurin assay was employed to determine the minimum inhibitory concentration(MIC90)of PVP against MDR and *** MIC90 ranged between 1.57μg/mL and 3.13μg/mL,which are significantly lower than many antimicrobials tested from natural sources against this *** spectrophotometrically determined growth analysis and total microscopic counts using Live/dead®BacLight™fluorescent stain exhibited a steady decrease in viability of both MDR and MRSA cultures following treatment with PVP at the MIC *** silico predictive molecular docking study revealed that PVP could be a DNA-targeting minor groove binding antimicrobial *** continued development of novel antimicrobials derived from natural sources with the combination of a suite of conventional antibiotics could stem the rising pandemic of MDR and MRSA along with other deadly microbial pathogens.

关键词： natural product bacterial pigment resazurin assay minimum inhibitory concentration(MIC)

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当前围手术期处理模式:北欧五国结直肠外科医生调查

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英国医学杂志中文版 2005年第6期8卷 339-341页

作者： Kristoffer Lassen Pascal Hannemann Olle Ljungqvist ken Fearon Cornelis H C Dejong Maarten F von Meyenfeldt Jonatan Hausel Jonas Nygren Jens andersen Arthur Revhaug 王东 Department of Gastrointestinal Surgery University Hospital of Northern Norway9038TromsφNorway Department of Surgery University Hospital MaastrichtMaastricht6202AZNetherlands Centre for Surgical Sciences Division of SurgeryKarolinska University HospitalStockholmSweden Clinical and Surgical Sciences(Surgery) School of Clinical Sciences and Community HealthUniversity of EdinburghEdinburgh EH16 4SAprofessor of surgery Department of Surgical Gastroenterology 435 Hvidovre University HospitalHvidovre 2650Denmark

有关结直肠外科围手术期最佳治疗的证据是很充足的.通过术后进食、静脉适量输液以及减少神经内分泌系统的应激反应,可以抑制术后分解代谢,同时促进患者的康复.术前肠道准备和术后胃肠减压这些特别措施已不作为常规,而改为术后持续硬膜... 详细信息

有关结直肠外科围手术期最佳治疗的证据是很充足的.通过术后进食、静脉适量输液以及减少神经内分泌系统的应激反应,可以抑制术后分解代谢,同时促进患者的康复.术前肠道准备和术后胃肠减压这些特别措施已不作为常规,而改为术后持续硬膜外麻醉、限制静脉输液和补钠、术后第1天开始即允许经口自由进食1-5等.本项调查的目的是了解北欧五国(苏格兰、荷兰、丹麦、瑞典和挪威)在结直肠癌手术围手术期治疗方面的特点.

关键词：围手术期处理结直肠外科医生北欧神经内分泌系统持续硬膜外麻醉术后进食外科围手术期术后胃肠减压术前肠道准备

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