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Preliminary exploration of animal models of congenital choledochal cysts

World Journal of Gastroenterology 2024年第10期30卷 1420-1430页

作者： Shu-Hao Zhang Yue-Bin Zhang Duo-Te Cai Tao Pan ken Chen Yi Jin Wen-juan Luo Zong-Wei Huang Qing-Jiang Chen Zhi-Gang Gao Department of General Surgery Children’s HospitalZhejiang University School of MedicineHangzhou 310000Zhejiang ProvinceChina

BACKGROUND Various animal models have been used to explore the pathogenesis of choledochal cysts(CCs),but with little convincing *** surgical techniques can achieve satisfactory outcomes for treatment of ***,recent studies have focused more on clinical issues rather than basic ***,we need appropriate animal models to further basic *** To establish an appropriate animal model that may contribute to the investigation of the pathogenesis of *** Eighty-four specific pathogen-free female Sprague-Dawley rats were randomly allocated to a surgical group,sham surgical group,or control group.A rat model of CC was established by partial ligation of the bile *** reliability of the model was confirmed by measurements of serum biochemical indices,morpho-logy of common bile ducts of the rats as well as molecular biology experiments in rat and human *** Dilation classified as mild(diameter,≥1 mm tof alanine aminotransferase,aspartate aminotrans-ferase,total bilirubin,direct bilirubin,and total bile acids were significantly elevated in the surgical group as compared to the control group 7 d after surgery,while direct bilirubin,total bilirubin,and gamma-glutamyltransferase were further increased 14 d after *** of the biochemical indices gradually decreased to normal ranges 28 d after *** protein expression trend of signal transducer and activator of transcription 3 in rat model was consistent with the human CC *** The model of partial ligation of the bile duct of juvenile rats could morphologically simulate the cystic or fusiform CC,which may contribute to investigating the pathogenesis of CC.

关键词： Choledochal cyst Animal model Partial ligation Cystic and fusiform dilation Juvenile rats

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宁夏枸杞果皮蜡质积累与气象因子的关系

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林业科学 2024年第3期60卷 22-34页

作者：米娟娟赵娟红李治刚包晗黄婷秦垦杨涓郑国琦宁夏大学生命科学学院林木资源高效生产全国重点实验室银川750021 宁夏农林科学院枸杞科学研究所银川750002

【目的】分析不同采摘时期枸杞果皮蜡质含量与成分差异,探究不同采摘时期气象因子与枸杞果皮蜡质积累的关系,为不同采摘时期适宜制干方式的选择提供理论依据。【方法】以‘宁杞1号’和‘宁杞5号’3个采摘时期(老眼枝时期、七寸枝时期和... 详细信息

【目的】分析不同采摘时期枸杞果皮蜡质含量与成分差异,探究不同采摘时期气象因子与枸杞果皮蜡质积累的关系,为不同采摘时期适宜制干方式的选择提供理论依据。【方法】以‘宁杞1号’和‘宁杞5号’3个采摘时期(老眼枝时期、七寸枝时期和秋果时期)的果实为材料,应用GC-MS技术对果皮蜡质含量、组分进行测定与分析,利用JL-18空气温、湿、光照记录仪监测不同采摘时期的气象因子,采用逐步回归分析法探究果皮蜡质含量及其组分与气象因子之间的关系。【结果】1)2个枸杞品种在3个采摘期的青果期、转色期和红果期果实蜡质含量均存在显著差异,红果期果实蜡质含量显著高于青果期和转色期,而青果期和转色期果实蜡质含量差异不显著。2)‘宁杞1号’的蜡质成分主要为二十六烷、二十八烷、1-碘代二十八烷、1-碘代三十烷、二十二醇和β-香树精,‘宁杞5号’的蜡质成分主要为二十六烷、二十七烷、1-碘代二十八烷、1-碘代三十烷、二十二醇和2-己基-1-癸醇。3)老眼枝时期,‘宁杞1号’和‘宁杞5号’果皮蜡质含量与光照强度显著负相关,相关系数分别为-0.759(P<0.05)和-0.838(P<0.01)。‘宁杞1号’酯类与日均温度显著负相关,相关系数为-0.712(P<0.05),‘宁杞5号’酸类和酯类与日均湿度显著正相关,相关系数分别为0.728(P<0.05)和0.739(P<0.05)。‘宁杞1号’和‘宁杞5号’酮类与夜均温度显著正相关,与光照强度显著负相关,相关系数分别为0.717(P<0.01)和-0.649(P<0.05);烷烃类与日均湿度和夜均温度显著负相关,相关系数分别为-0.772(P<0.01)和0.637(P<0.05);碘代烷烃类与夜均湿度显著正相关,相关系数为0.820(P<0.01)。七寸枝时期,‘宁杞1号’烷烃类与夜均温度显著正相关,相关系数为0.779(P<0.05);碘代烷烃类与日均温度显著负相关,相关系数为-0.724(P<0.05);酸类与日均湿度显著负相关,相关系数为-0.680(P<0.05)。‘宁杞5号’蜡质含量与夜均湿度和光照强度显著正相关,相关系数分别为0.795(P<0.01)和0.748(P<0.01);酮类和碘代烷烃类与日均温度显著负相关,相关系数分别为-0.887(P<0.01)和-0.749(P<0.05)。秋果时期,‘宁杞1号’烷烃类和碘代烷烃类与夜均温度显著正相关,相关系数分别为0.887(P<0.01)和0.767(P<0.05)。【结论】不同采摘时期的气象因子对宁夏枸杞果皮蜡质含量与成分产生不同影响,温度与碘代烷烃类、酮类和烷烃类积累有关,湿度与酯类、酸类和烷烃类积累有关,光照强度与酯类、醇类积累有关。

关键词：宁夏枸杞不同采摘时期蜡质积累蜡质组分气象因子

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宁夏枸杞果实性状和预处理对果实制干的影响

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林业科学 2024年第3期60卷 35-44页

作者：赵娟红米娟娟李治刚包晗黄婷秦垦杨涓郑国琦宁夏大学生命科学学院林木资源高效生产全国重点实验室银川750021 宁夏农林科学院枸杞科学研究所银川750002

【目的】探究不同品种(系)宁夏枸杞果实性状和预处理对果实制干的影响,为枸杞制干工艺提供有价值的理论依据。【方法】以制干差异较大的不同品种(系)宁夏枸杞为试验材料,采用称重法、石蜡切片和扫描电镜等方法,对果实加工性状、果皮及... 详细信息

【目的】探究不同品种(系)宁夏枸杞果实性状和预处理对果实制干的影响,为枸杞制干工艺提供有价值的理论依据。【方法】以制干差异较大的不同品种(系)宁夏枸杞为试验材料,采用称重法、石蜡切片和扫描电镜等方法,对果实加工性状、果皮及角质层结构、果皮蜡质微形态和不同预处理枸杞果实制干时间进行探究,明确不同品种(系)宁夏枸杞果实性状和预处理对果实制干的影响。【结果】1)不同品种(系)枸杞果实纵径、百粒质量、单果体积存在显著差异,‘16-23-7-8’横径、纵径、百粒质量、单果体积均最高,‘宁杞1号’最低。2)随着果实发育,果皮和角质层厚度逐渐增加且存在显著差异。‘宁杞5号’果皮厚度最大,达768.273μm;‘宁杞1号’果皮厚度最小,为445.100μm。‘14-402’角质层最厚,达9.420μm;‘16-23-7-8’最低,为7.528μm。3)成熟期枸杞果实表皮被束状蜡质层覆盖,蜡质晶体呈无规则片状,片状蜡质层较厚,排列较紧密。4)不同品种(系)枸杞果实制干时间存在显著差异,由易到难依次为‘宁杞1号’、‘Z44’、‘16-23-7-8’、‘14-402’、‘宁杞5号’。与对照相比,碱处理后,平均制干时间缩短4.10 h;氯仿处理后,平均制干时间缩短16.94 h。【结论】枸杞果皮结构通过影响水分运输进而影响枸杞果实制干过程;预处理可破坏枸杞表皮蜡质层缩短制干时间。本研究5个枸杞品种(系)中,‘宁杞5号’不易制干,‘宁杞1号’最易制干,在枸杞制干时,应用的预处理方式必须考虑品种间存在的组织和形态解剖学特征的差异。

关键词：宁夏枸杞果实性状果皮结构角质层表皮蜡质预处理制干时间

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Landscape genetics reveals inbreeding and genetic bottlenecks in the extremely rare short-globose cacti Mammillaria pectinifera (Cactaceae) as a result of habitat fragmentation

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Plant Diversity 2017年第1期39卷 13-19页

作者： Reyna Maya-Garcia Santiago Arizaga Pablo Cuevas-Reyes juan Manuel Penaloza-Ramirez Victor Rocha Ramirez ken Oyama facultad de Biologia Universidad Michoacana de San Nicolas de Hidalgo Mexico Escuela Nacional-de Estudios Superiores Unidad Morelia UNAM Antigua Carretera a Pdtzcuaro 8701 Colonia Ex-Hacienda de SanJose de la Huerta58190 Morelia Michoacan Mexico

Mammillaria pectinifera is an endemic, short-globose cactus species, included in the IUCN list as a threatened species with only 18 remaining populations in the Tehuacfin-Cuicatlan Valley in central Mexico. We evaluated the population genetic diversity and structure, connectivity, recent bottlenecks and population size, using nuclear microsatellites. M. pectinifera showed high genetic diversity but some evidence of heterozygote deficiency （f15）, recent bottlenecks in some populations and reductions in population size. Also, we found low population genetic differentiation and high values of connectivity for M. pectinifera, as the result of historical events of gene flow through pollen and seed dispersal. M. pectinifera occurs in sites with some degree of disturbance leading to the isolation of its populations and decreasing the levels of gene flow among them. Excessive deforestation also changes the original vegetation damaging the natural habitats. This species will become extinct if it is not properly preserved. furthermore, this species has some ecological features that make them more vulnerable to disturbance such as a very low growth rates and long life cycles. We suggest in situ conservation to prevent the decrease of population sizes and loss of genetic diversity in the natural protected areas such as the Tehuacan-Cuicatlan Biosphere Reserve. In addition, a long-term ex situ conservation program is need to construct seed banks, and optimize seed germination and plant establishment protocols that restore disturbed habitats. furthermore, creating a supply of living plants for trade is critical to avoid further extraction of plants from nature.

关键词： MammiUaria pectiniferaNuclear SSRGenetic diversityRecent bottlenecksEffective population size

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Visual perception alterations in COVID-19:a preliminary study

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International Journal of Ophthalmology(English edition) 2023年第1期16卷 1-9页

作者： María Begoña Coco-Martín Luis Leal-Vega Irene Alcoceba-Herrero Ainhoa Molina-Martín Dolores de-fez María JoséLuque Carlos Dueñas-Gutiérrez juan francisco arenillas-Lara David PPiñero Group of Applied Clinical Neurosciences and Advanced Data Analysis Department of MedicineDermatology and ToxicologyUniversity of ValladolidValladolid 47005Spain Group of Optics and Visual Perception Department of OpticsPharmacology and AnatomyUniversity of AlicanteAlicante 03690Spain Department of Optics and Optometry and Vision SciencesUniversity of ValenciaValencia 46100Spain COVID-19 Unit Department of Internal MedicineUniversity Clinical Hospital of ValladolidValladolid 47003Spain Stroke Unit Department of NeurologyUniversity Clinical Hospital of ValladolidValladolid 47003Spain Clinical Optometry Unit Department of OphthalmologyVithas Medimar International HospitalAlicante 03016Spain

AIM:To compare the visual perception(color and chromatic-achromatic contrast vision)of a small cohort of COVID-19 patients at the time of infection and after 6mo with that of a healthy population matched for sex and ***:A total of 25 patients(9 females,16 males,mean age:54±10y)with COVID-19 hospitalized in the COVID-19 Unit of the University Clinical Hospital of Valladolid were recruited for this preliminary *** perception,as determined by monocular measurement of contrast sensitivity function(CSf)and color vision was assessed in each patient using the Optopad *** results obtained were then compared with those of a sample of 16 age-and sex-matched healthy controls(5 females,11 males,mean age:50±6y)in which the same measurement procedure was *** significant differences between groups were assessed using the Mann-Whitney U *** were repeated after a minimum follow-up period of 6mo and statistically significant differences between the two time points in each group were assessed using the Wilcoxon signed rank ***:Discrimination thresholds(color and chromatic-achromatic contrast vision)and their corresponding sensitivity,calculated as the inverse of the discrimination threshold,were *** of the data revealed higher contrast threshold results(i.e.,worse contrast sensitivity)in the COVID-19 group than in the control group for all spatial frequencies studied in the Optopad-CSf achromatic test and most of the spatial frequencies studied in the Optopad-CSf chromatic test for the red-green and blue-yellow *** addition,color threshold results in the COVID-19 group were also significantly higher(i.e.,worse color sensitivity)for almost all color mechanisms studied in the Optopad-Color *** 6mo,most of the differences found between the groups were maintained despite COVID-19 ***:The present results provide preliminary evidence that visual perception may be impaired in COVID-19

关键词： SARS-CoV-2 COVID-19 visual perception contrast sensitivity function color vision

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The Construction of the Probe for Amylase n Gene Cloning from Bacillus halodurans Strain 38C1-1

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Journal of Northeast Agricultural University(English Edition) 2005年第2期12卷 108-111页

作者： QU juan-juan YANG Guo-hui Aoki ken-ji College of Resource and Environment Northeast Agricultural University Harbin tteilongjiang 150030 PRC Agriculture faculty Kobe University Kobe 657-8501Japan

Primers and probes were established according to the sequences of the alpha-amylase genes of Bacillus. halodurans C-125, Therrnus sp. IM6501, B. stearothermophilus ET-1, and B, acidopullulytics. Primers were designed and a 0.2 kb DNA fragment was amplified, the fragment was successfully used for the detection of the amylase Ⅱ gene in a 2 842 bp region from Bacillus halodurans strain 38C1-1.

关键词： probe amylase Ⅱ gene cloning

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Biophysical warming patterns of an open-top chamber and its short-term influence on a Phragmites wetland ecosystem in China

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China Geology 2023年第4期6卷 594-610页

作者： Xue-yang Yu Si-yuan Ye Li-xin Pei Liu-juan Xie ken W.Krauss Samantha K.Chapman Hans Brix Key Laboratory of Coastal Wetland Biogeosciences Qingdao Institute of Marine GeologyChina Geological SurveyMinistry of Natural ResourcesQingdao 266071China Institute of Marine Science and Technology Shandong UniversityQingdao 266237China Wetland and Aquatic Research Center U.S.Geological SurveyLafayette 70506United States of America Department of Biology Villanova UniversityVillanova 19085United States of America Department of Biology Aarhus UniversityAarhus 8000CDenmark

Passive-warming, open-top chambers(OTCs) are widely applied for studying the effects of future climate warming on coastal wetlands. In this study, a set of six OTCs were established at a Phragmites wetland located in the Yellow River Delta of Dongying City, China. With data collected through online transmission and in-situ sensors, the attributes and patterns of realized OTCs warming are *** authors also quantified the preliminary influence of experimental chamber warming on plant *** produced an elevated average air temperature of 0.8°C(relative to controls) during the growing season(June to October) of 2018, and soil temperatures actually decreased by 0.54°C at a depth of 5 cm and 0.46°C at a depth of 30 cm in the OTCs. Variations in diel patterns of warming depend greatly on the heat sources of incoming radiation in the daytime versus soil heat flux at night. Warming effects were often larger during instantaneous analyses and influenced OTCs air temperatures from-2.5°C to 8.3°C dependent on various meteorological conditions at any given time, ranging from cooling influences from vertical heat exchange and vegetation to radiation-associated warming. Night-time temperature depressions in the OTCs were due to the low turbulence inside OTCs and changes in surface soilatmosphere heat transfer. Plant shoot density, basal diameter, and biomass of Phragmites decreased by23.2%, 6.3%, and 34.0%, respectively, under experimental warming versus controls, and plant height increased by 4.3%, reflecting less carbon allocation to stem structures as plants in the OTCs experienced simultaneous wind buffering. While these passive-warming OTCs created the desired warming effects both to the atmosphere and soils, pest damages on the plant leaves and lodging within the OTCs were extensive and serious, creating the need to consider control options for these chambers and the replicated OTCs studies underway in other Chinese Phragmites marshes(Panjin and Yancheng).

关键词： Open-top chambers(OTCs)warming Phragmites australis wetland Short-term ecosystem impact Climate warming Soil heat flux Soil-atmosphere heat transfer Ecological geological engineering Hydrogeological engineering Yellow River Delta

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脑机接口范式及其脑电数据集研究进展

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医学研究与教育 2024年第4期41卷 21-29页

作者：王井辉高娟李恳王子文徐玉洁任涵李泽光张雨婷承德医学院研究生学院河北承德067000 河北省分子病理与肿瘤早期诊断重点实验室河北保定071000 保定市第一中心医院神经内科河北保定071000 河北医科大学研究生学院河北石家庄050000

脑机接口(brain-computer interface,BCI)是指人脑与外部设备之间创建直接连接,实现脑与设备的信息交换。临床医生可以使用不同方法监测大脑活动,脑电图已被用作测量大脑活动的最常用方法,具有高时间分辨率、便携性和易用性。对于患有... 详细信息

脑机接口(brain-computer interface,BCI)是指人脑与外部设备之间创建直接连接,实现脑与设备的信息交换。临床医生可以使用不同方法监测大脑活动,脑电图已被用作测量大脑活动的最常用方法,具有高时间分辨率、便携性和易用性。对于患有严重运动障碍的人,BCI成为一种可行的人机界面,可以让这些患者与外界互动,帮助改善他们的生活质量。它与传统的康复方式不同,可以充分调动患者的训练积极性。综述近年来BCI领域的各项研究成果以及脑电数据集,希望能促进神经科学中脑卒中康复领域的发展。

关键词：脑机接口脑电图数据集脑卒中运动想象

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首富没落巴西石油大亨巴蒂斯塔一年之内巨亏345亿美元始末

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商业周刊（中文版） 2013年第21期 102-107页

作者： juan PABLO SPINETTO PETER MILLARD ken WELLS

2012年4月的一个午后，巴西亿万富翁埃克·巴蒂斯塔（Eike Batista）登上了阿苏港项目区一个特制舞台。

关键词：斯塔巴西没落首富美元巨亏石油亿万富翁

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子集和问题的O（1．414^n）链数DNA计算机算法

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计算机学报 2007年第11期30卷 1947-1953页

作者：李肯立姚凤娟许进李仁发湖南大学计算机与通信学院长沙410082 华中科技大学分子生物计算机研究所武汉430074

随着DNA计算机研究的不断深入,如何克服DNA生物计算中穷举法的极限已成为DNA计算研究的重要内容之一.为设计可扩展的子集和问题DNA计算机算法,文中将Aldeman-Lipton模型的操作与粘贴模型的解空间结合,引入荧光标记和凝胶电泳技术,通过设... 详细信息

随着DNA计算机研究的不断深入,如何克服DNA生物计算中穷举法的极限已成为DNA计算研究的重要内容之一.为设计可扩展的子集和问题DNA计算机算法,文中将Aldeman-Lipton模型的操作与粘贴模型的解空间结合,引入荧光标记和凝胶电泳技术,通过设计DNA并行搜索器,提出一种求解子集和问题的DNA计算机模型和算法.与已有文献结论的对比分析表明:文中算法在保持多项式生物操作复杂性的条件下,将穷举算法中的DNA分子链数从O(2n)减少至O(1.414n),其中n为子集和问题的维数.因此,文中算法理论上在试管级生化反应条件下能将可破解子集和公钥的维数从60提高到120.

关键词： DNA计算子集和问题分治法并行处理 NP完全问题

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