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单细胞RNA测序技术探究CCN2基因在特纳综合征胎儿颈部淋巴水囊瘤中的关键作用

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中华细胞与干细胞杂志（电子版） 2023年第4期13卷 220-228页

作者：李颖思符芳杨昕邓琼周航程肯李东至廖灿广州医科大学附属广州市妇女儿童医疗中心产前诊断中心 510623 华南理工大学广州510641

目的应用单细胞RNA测序技术(scRNA-seq)探讨细胞通讯网络因子2(CCN2,也称为CTGF)在特纳综合征(TS)胎儿颈部淋巴水囊瘤(CH)的潜在发生机制。方法收集2020年1月至2020年12月于广州市妇女儿童医疗中心产前诊断中心就诊,孕11~13^(+6)周经腹... 详细信息

目的应用单细胞RNA测序技术(scRNA-seq)探讨细胞通讯网络因子2(CCN2,也称为CTGF)在特纳综合征(TS)胎儿颈部淋巴水囊瘤(CH)的潜在发生机制。方法收集2020年1月至2020年12月于广州市妇女儿童医疗中心产前诊断中心就诊,孕11~13^(+6)周经腹部超声检查诊断为CH而终止妊娠及相同孕周因社会因素终止妊娠病例,产前未行遗传学检测者,需同时取引产胎儿胎盘绒毛组织或骨骼肌,按照标准的操作流程进行遗传学检测。全部病例应用遗传学检测确定染色体异常类型后,CH病例中选择遗传学结果为45,X0的胎儿作为实验组,无CH病例中选择遗传学结果为46,XN的胎儿作为对照组。本研究共纳入6例样本,实验组3例,对照组3例。所有入组病例在终止妊娠后立即取颈后皮肤组织,一部分样本应用HE染色进行病理分析,一部分应用scRNA-seq分析颈后皮肤组织细胞类型并建立差异基因表达谱,并进一步应用RT-qPCR检测细胞中基因表达水平及Western blot检测相关蛋白表达情况以明确单细胞测序结果的可靠性。实验组与对照组采用独立样本t检验进行统计学分析。结果病理结果提示实验组表现为淋巴管增生和扩张,淋巴结增多坏死,血管减少,间质纤维丝束增多、变长,并出现大量细胞浸润组织间隙。应用scRNA-seq分析CH病例,共捕获细胞54488个,26个聚类,其中成纤维细胞占23.1%。同时发现基质细胞蛋白CCN2高表达。进一步定位分析显示实验组中CCN2主要高表达在成纤维细胞、内皮细胞、组织干细胞和间充质干细胞。同时Western blot验证CCN2蛋白在实验组中高表达(2.47±0.49比1.00±0.08,P<0.05)。结论病理结果表明淋巴管增生扩张和淋巴结增多坏死等淋巴管发育异常是TS胎儿CH的病理生理学基础。scRNA-seq结果表明基质细胞蛋白CCN2异常高表达可能导致TS胎儿CH。

关键词：特纳综合征淋巴水囊瘤单细胞测序成纤维细胞 CCN2

ERP系统在苏里格气田低成本战略中的应用探析

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低渗透油气田 2018年第1期23卷 129-132页

作者：柳肯孟凡伟王建民于雷吕碧军翟小强王斌中国石油长庆油田分公司第三采气厂陕西燃气集团有限公司中国石油长庆油田分公司第三采油厂

为了实现苏里格气田经济有效开发，低成本战略是优选，ERP系统引入应用势在必行。苏里格气田在项目立项审查、投资计划下达、信息共享方面需要不断完善，通过ERP系统在苏里格气田项目投资管理决策、实施等方面的应用，不断完善ERP系统... 详细信息

为了实现苏里格气田经济有效开发，低成本战略是优选，ERP系统引入应用势在必行。苏里格气田在项目立项审查、投资计划下达、信息共享方面需要不断完善，通过ERP系统在苏里格气田项目投资管理决策、实施等方面的应用，不断完善ERP系统功能、建立配套的管理和考核制度、调整管理方式，积极应对ERP系统运行项目管理的新变化，进一步推进苏里格气田项目管理的科学规范运行，助力实现气田经济有效开发。

关键词： ERP系统苏里格气田低成本战略

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气液两相分离集成装置研制及应用

低渗透油气田 2018年第2期23卷 67-70页

作者：赵卫军柳肯李国聪贾永社常家豪李文鹏中国石油长庆油田分公司第三采气厂

在试气工艺中,放喷排液是一个至关重要的环节,直接影响试气效果及产气量。传统试气工艺存在环境安全风险,通过对传统和现有试气工艺存在问题进行分析,设计制造了气液两相分离集成装置。该装置主要由除砂器、气液分离器、防爆型储液罐3... 详细信息

在试气工艺中,放喷排液是一个至关重要的环节,直接影响试气效果及产气量。传统试气工艺存在环境安全风险,通过对传统和现有试气工艺存在问题进行分析,设计制造了气液两相分离集成装置。该装置主要由除砂器、气液分离器、防爆型储液罐3部分组成。实验表明,该装置节能效果良好,具有低能耗、低成本、环境友好、安全可靠等优点,推广应用价值较好。

关键词：试气放喷分离器节能环保

Decline in subarachnoid haemorrhage volumes associated with the first wave of the COVID- 19 pandemic

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Stroke & Vascular Neurology 2021年第4期6卷 542-552,I0026-I0032页

作者： Thanh N Nguyen Diogo C Haussen Muhammad M Qureshi Hiroshi Yamagami Toshiyuki Fujinaka Ossama Y Mansour Mohamad Abdalkader Michael Frankel Zhongming Qiu Allan Taylor Pedro Lylyk Omer F Eker Laura Mechtouff Michel Piotin Fabricio Oliveira Lima Francisco Mont'Alverne Wazim Izzath Nobuyuki Sakai Mahmoud Mohammaden Alhamza R Al-Bayati Leonardo Renieri Salvatore Mangiafico David Ozretic Vanessa Chalumeau Saima Ahmad Umair Rashid Syed Irteza Hussain Seby John Emma Griffin John Thornton Jose Antonio Fiorot Rodrigo Rivera Nadia Hammami Anna M Cervantes-Arslanian Hormuzdiyar H Dasenbrock Huynh Le Vu Viet Quy Nguyen Steven Hetts Romain Bourcier Romain Guile Melanie Walker Malveeka Sharma Don Frei Pascal Jabbour Nabeel Herial Fawaz Al-Mufti Atilla Ozcan Ozdemir Ozlem Aykac Dheeraj Gandhi Chandril Chugh Charles Matouk Pascale Lavoie Randall Edgell Andre Beer-Furlan Michael Chen Monika Killer-Oberpfalzer Vitor Mendes Pereira Patrick Nicholson Vikram Huded Nobuyuki Ohara Daisuke Watanabe Dong Hun Shin Pedro SC Magalhaes Raghid Kikano Santiago Ortega-Gutierrez Mudassir Farooqui Amal Abou-Hamden Tatsuo Amano Ryoo Yamamoto Adrienne Weeks Elena A Cora Rotem Sivan-Hoffmann Roberto Crosa Markus Möhlenbruch Simon Nagel Hosam Al-Jehani Sunil A Sheth Victor S Lopez Rivera James E Siegler Achmad Fidaus Sani Ajit S Puri Anna Luisa Kuhn Gianmarco Bernava Paolo Machi Daniel G Abud Octavio M Pontes-Neto Ajay K Wakhloo Barbara Voetsch Eytan Raz Shadi Yaghi Brijesh P Mehta Naoto Kimura Mamoru Murakami Jin Soo Lee Ji Man Hong Robert Fahed Gregory Walker Eiji Hagashi Steve M Cordina Hong Gee Roh ken Wong Juan F Arenillas Mario Martinez-Galdamez Jordi Blasco Alejandro Rodriguez Vasquez Luisa Fonseca M Luis Silva Teddy Y Wu Simon John Alex Brehm Marios Psychogios William J Mack Matthew Tenser Tatemi Todaka Miki Fujimura Roberta Novakovic Jun Deguchi Yuri Sugiura Hiroshi Tokimura Rakesh Khatri Michael Kelly Lissa Peeling Yuichi Murayama Hugh Stephen Winters Johnny Wong Mohamed Teleb Jeremy Payne Hiroki Fukuda Kosuke Miyake Junsuke Shimbo Yusuke Sug Neurology RadiologyBoston Medical CenterBostonMassachusettsUSA Neurology Marcus Stroke&Neuroscience CenterGrady Memorial HospitalEmory University School of MedicineAtlantaGAUSA Radiology Radiation OncologyBoston University School of MedicineBostonMassachusettsUSA Neurology National Hospital Organization Osaka National HospitalOsakaJapan Neurosurgery National Hospital Organization Osaka National HospitalOsakaJapan Neurology Alexandria UniversityAlexandriaEgypt Radiology Boston Medical CenterBostonMassachusettsUSA Department of Neurology Xinqiao HospitalChongqingChina Neurosurgery University of Cape TownRondeboschWestern CapeSouth Africa Neurosurgery Interventional NeuroradiologyClinica La Sagrada FamiliaBuenos AiresArgentina Neuroradiologie Neurologie VasculaireHospices Civils de LyonLyonAuvergne-Rhône AlpesFrance Neurologie Vasculaire Hospices Civils de LyonLyonAuvergne-Rhône AlpesFrance Interventional Neuroradiology Fondation Ophtalmologique Adolphe de RothschildParisÎle-de FranceFrance Neurology Hospital Geral de FortalezaFortalezaBrazil Interventional Neuroradiology Hospital Geral de FortalezaFortalezaBrazil Neuroradiology Nottingham University Hospitals NHS TrustNottinghamUK Department of Neurosurgery Kobe City Medical Center General HospitalKobeHyogoJapan Interventional Neurovascular Unit University Hospital CareggiFirenzeToscanaItaly Neuroradiology University Hospital Centre ZagrebZagrebCroatia Interventional Neuroradiology Hopital BicetreLe Kremlin-BicetreFrance Stroke and Interventional Neuroradiology Lahore General HospitalLahorePakistan Neurological Institute Cleveland Clinic Abu DhabiAbu DhabiUAE Department of Radiology Beaumont HospitalDublinIreland Neurology Stroke UnitHospital-Estadual CentralVitoriaBrazil Neuroradiology Instituto de Neurocirugia Dr AsengoSantiagoChile Interventional Neuroradiology Institut National de NeurologieTunisTunisia Neurology Boston Medical CenterBostonMassachusettsUSA Neurosurgery Boston Medical Center

Background During the COVID-19 pandemic,decreased volumes of stroke admissions and mechanical thrombectomy were *** study’s objective was to examine whether subarachnoid haemorrhage(SAH)hospitalisations and ruptured aneurysm coiling interventions demonstrated similar *** We conducted a cross-sectional,retrospective,observational study across 6 continents,37 countries and 140 comprehensive stroke *** with the diagnosis of SAH,aneurysmal SAH,ruptured aneurysm coiling interventions and COVID-19 were identified by prospective aneurysm databases or by International Classification of Diseases,10th Revision,*** 3-month cumulative volume,monthly volumes for SAH hospitalisations and ruptured aneurysm coiling procedures were compared for the period before(1 year and immediately before)and during the pandemic,defined as 1 March-31 May *** prior 1-year control period(1 March-31 May 2019)was obtained to account for seasonal *** There was a significant decline in SAH hospitalisations,with 2044 admissions in the 3 months immediately before and 1585 admissions during the pandemic,representing a relative decline of 22.5%(95%CI−24.3%to−20.7%,p<0.0001).Embolisation of ruptured aneurysms declined with 1170-1035 procedures,respectively,representing an 11.5%(95%CI−13.5%to−9.8%,p=0.002)relative *** analysis was noted for aneurysmal SAH hospitalisation decline from 834 to 626 hospitalisations,a 24.9%relative decline(95%CI−28.0%to−22.1%,p<0.0001).A relative increase in ruptured aneurysm coiling was noted in low coiling volume hospitals of 41.1%(95%CI 32.3%to 50.6%,p=0.008)despite a decrease in SAH admissions in this *** There was a relative decrease in the volume of SAH hospitalisations,aneurysmal SAH hospitalisations and ruptured aneurysm embolisations during the COVID-19 *** findings in SAH are consistent with a decrease in other emergencies,such as stroke and myocardial infarction.

关键词： diagnosis representing subarachnoid