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*** hypertension promotes early mitochondrial fragmentation in retinal endothelial cells in a mouse model of glaucoma

Annals of Eye Science 2018年第1期 424-424页

作者： Jorge L.Cueva Vargas Yoko Ito Khalil miloudi Ariel m.Wilson Christine Vande Velde Przemyslaw Sapieha Adriana Di Polo Department of Neuroscience University of Montreal Hospital Research Center(CR-CHUM)MontrealQuebecCanada Department of Biochemistry Maisonneuve-Rosemont Hospital Research CentreUniversity of MontrealMontrealQuebecCanada Department of Ophthalmology Maisonneuve-Rosemont Hospital Research CentreUniversity of MontrealMontrealQuebecCanada Department of Neuroscience McGill UniversityMontrealQuebecCanada

Background:Retinal endothelial cells are very active and contribute to the integrity of the neurovascular *** dysfunction has been proposed to contribute to the pathogenesis of ***,we evaluated the hypothesis that ocular hypertension triggers mitochondrial alterations in endothelial cells impairing the integrity of the blood retinal barrier(BRB).methods:Ocular hypertension was induced by injection of magnetic microbeads into the anterior chamber of Endomito-EGFP mice,a strain expressing green fluorescent protein selectively in the mitochondria of endothelial *** density,mitochondrial volume,and the number of mitochondrial components were quantified in 3D-reconstructed images from whole-mounted retinas using Imaris ***-related protein(DRP-1),mitofusin-2(mFN-2)and optic atrophy-1(OPA-1)expression were assessed by western blot analysis of enriched endothelial *** structure was evaluated by transmission electron microscopy(TEm)and oxygen consumption rate was monitored by Seahorse *** integrity of the BRB was evaluated by quantifying evans blue ***:Our data demonstrate that two and three weeks after ocular hypertension induction,the total mitochondria volume in endothelial cells decreased from 0.140±0.002µm3 from non-injured retinas to 0.108±0.005 and 0.093±0.007µm3,respectively in glaucomatous eyes(mean±S.E.m,ANOVA,Pmaller mitochondria complexes(m3)in endothelial cells from glaucomatous *** upregulation of DRP-1 was found in vessels isolated from glaucomatous retinas compared to the intact retinas,while mFN-2 and OPA-1 expression was not *** alteration in endothelial cell mitochondria was confirmed by TEm,which were accompanied by a 1.93-fold reduction in the oxygen consumption rate as well as 2.6-fold increase in vasculature leakage in glaucomatous retinas(n=3-6/group).In additi

关键词： Glaucoma endothelial cells mitochondria vascular leakage

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