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Retinoic acid signaling acts as a rheostat to balance Treg function

Cellular & Molecular Immunology 2022年第7期19卷 820-833页

作者： Govindarajan Thangavelu Gabriela Andrejeva Sara Bolivar-Wagers Sujeong Jin Michael C.Zaiken Michael loschi Ethan G.Aguilar Scott N.Furlan Chrysothemis C.Brown Yu-Chi lee Cameron McDonald Hyman Colby J.Feser Angela Panoskaltsis-Mortari keli l.hippen Kelli P.MacDonald William J.Murphy Ivan Maillard Geoffrey R.Hill David H.Munn Robert Zeiser leslie S.Kean Jeffrey C.Rathmell Hongbo Chi Randolph J.Noelle Bruce R.Blazar Department of Pediatrics Center for ImmunologyUniversity of MinnesotaMinneapolisMNUSA Center for Immunobiology Vanderbilt University Medical CenterNashville TNUSA Department of Pediatrics University of WashingtonSeattleWAUSA Fred Hutchinson Cancer Research Center SeattleWAUSA Howard Hughes Medical Institute Immunology Program and Ludwig CenterSloan Kettering InstituteMemorial Sloan Kettering Cancer CenterNew YorkNYUSA Department of Microbiology and Immunology.Geisel School of Medicine at Dartmouth Norris Cotton Cancer CenterLebanonUSA Department of Immunology Queensland Institute of Medical Research(QIMR)Berghofer Medical Research Institute and School of MedicineUniversity of QueenslandBrisbaneQLDAustralia Departmentof Dermatology School of MedicineUniversity of California DavisSacramentoCAUSA Division of Hematology/Oncology Department of MedicineUniversity of Pennsylvania Perelman School of MedicinePhiladelphiaPAUSA Georgia Cancer Center Augusta UniversityAugustaGAUSA Department of Haematology Oncology and Stem Cell TransplantationFaculty of MedicineFreiburg University Medical CentreFreiburgGermany Boston Children's Hospital and the Dana-Farber Cancer Institute BostonMAUSA Department of Immunology St.Jude Children's ResearchHospitalMemphisTNUSA

Regulatory T cells(Tregs)promote immune homeostasis by maintaining self-tolerance and regulating inflammatory *** certain inflammatory conditions,Tregs can lose their lineage stability and *** studies have reported that ex vivo exposure to retinoic acid(RA)enhances Treg function and ***,it is unknown how RA receptor signaling in Tregs influences these processes in ***,we employed mouse models in which RA signaling is silenced by the expression of the dominant negative receptor(DN)RARαin all T *** the fact that DNRARαconventional T cells are hypofunctional,Tregs had increased CD25 expression,STAT5 pathway activation,mTORC1 signaling and supersuppressor ***,DNRARαTregs had increased inhibitory molecule expression,amino acid transporter expression,and metabolic fitness and decreased antiapoptotic *** function was observed when wild-type mice were treated with a pharmacologic pan-RAR ***,Treg-specific expression of DNRARαresulted in distinct phenotypes,such that a single allele of DNRARαin Tregs heightened their suppressive function,and biallelic expression led to loss of suppression and *** loss of Treg function was not cell intrinsic,as Tregs that developed in a noninflammatory milieu in chimeric mice reconstituted with DNRARαand wild-type bone marrow maintained the enhanced suppressive *** mapping suggested that maintaining Treg stability in an inflammatory milieu requires RA *** findings indicate that RA signaling acts as a rheostat to balance Treg function in inflammatory and noninflammatory conditions in a dose-dependent manner.

关键词： Retinoic acid Tregulatory cells Autoimmunity Metabolism mTORC1 STAT5

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Comparison of Statistical Signal Detection Methods in Adverse Events Following Immunization — China, 2011–2015

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China CDC weekly 2024年第16期6卷 350-356,I0001页

作者： lanfang Xia keli li Yan li Zhijie An Quanwei Song lei Wang Zundong Yin Huaqing Wang National Key laboratory of Intelligent Tracking and Forecasting for Infectious Diseases(NITFID) BeijingChina National Immunization Program Chinese Center for Disease Control and PreventionBeijingChina

Introduction:The current study aims to assess the performance of data mining techniques in detecting safety signals for adverse events following immunization(AEFI)using routinely obtained data in *** different methods for detecting vaccine safety signals were ***:The AEFI data from 2011 to 2015 was collected for our *** analyzed the data using four different methods to detect signals:the proportional reporting ratio(PRR),reporting odds ratio(ROR),Bayesian confidence propagation neural network(BCPNN),and multi-item gamma Poisson shrinker(MGPS).Each method was evaluated at 1–3 thresholds for *** assess the performance of these methods,we used the published signal rates as gold standards to determine the sensitivity and ***:The number of identified signals varied from 602 for PRR1(with a threshold of 1)to 127 for *** considering the common reactions as the reference standard,the sensitivity ranged from 0.9%for MGPS1/2 to 38.2%for PRR1/2,and the specificity ranged from 85.2%for PRR1 and ROR1 to 96.7%for *** considering the rare reactions as the reference standard,PRR1,PRR2,ROR1,ROR2,and BCPNN exhibited the highest sensitivity(73.3%),while MGPS1 exhibited the highest specificity(96.9%).Discussion:For common reactions,the sensitivities were modest and the specificities were *** rare reactions,both the sensitivities and specificities were *** study provides valuable insights into the selection of signal detection methods and thresholds for AEFI data in China.

关键词： specificity routine vaccine

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Human CD39hi regulatory T cells present stronger stability and function under inflammatory conditions

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Cellular & Molecular Immunology 2017年第6期14卷 521-528页

作者： Jian Gu Xuhao Ni Xiongxiong Pan Hao lu Yunjie lu Jie Zhao Song Guo Zheng keli l.hippen Xuehao Wang ling lu Translational Medicine Research Center Affiliated Jiangning Hospital and Liver Transplantation Center First Affiliated Hospital Nanjing Medical University Nanjing 210029 China University of Minnesota Cancer Center and Division of Bone Marrow Transplantation Department of Pediatrics University of Minnesota Minneapolis MN 55455 USA Division of Rheumatology Penn State Milton S. Hershey Medical Center Hershey PA 17033 USA

Autoimmune diseases are characterized by an imbalance between regulatory T cells and effector T-cell subsets, such as Thl and Th17 cells. Studies have confirmed that natural CD4＋Foxp3＋ Tregs were unstable and dysfunctional in the presence of pro-inflammatory cytokines. In the current study, human CD39hi Tregs and CD39low Tregs were sorted from Tregs in vitro after 7 days of expansion. The functions of both Treg subsets were investigated under inflammatory conditions in vitro and in vivo. In the presence of Il-1β and Il-6, cultured CD4＋CD39hi Tregs maintained stable forkhead box protein 3 expression, whereas CD4＋CD39low Tregs lost Foxp3 expression and trans-differentiated into Thl or Th17 cells. Decreased Il-1βR and Il-6R expression on the CD39hi Tregs was the primary mechanism responsible for Treg stability. In addition, reduced activation of downstream molecules, such as STAT1 and STAT3, through the modulation of CpG demethylation played an important role. Finally, human CD4＋CD39hi Tregs but not CD4＋CD39low Tregs protected against xenograft versus host disease in model mice. These results strongly implied the physiological importance of CD39 expression and suggested that manipulation of CD39hi Tregs might represent a novel strategy for the treatment of autoimmune diseases.

关键词： CD39 stability Tregs xeno-GVHD

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基于苯并吡喃点亮型硫化氢荧光探针在食品检测中的应用与细胞成像

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有机化学 2024年第6期44卷 2027-2032页

作者：刘琳陈琳胡晓玲钟克利张璟琳汤立军渤海大学化学与材料工程学院辽宁锦州121013 辽宁省检验检测认证中心沈阳110000 北京工商大学中国轻工业酿酒分子工程重点实验室北京100048

硫化氢(H2S)不仅参与人体生理调节,也对酒的风味和口感有影响.合成了一种2,4-二硝基苯修饰的苯并吡喃衍生物(DCM-dip),该化合物在二甲基亚砜(DMSO)/磷酸缓冲溶液(PBS)(V:V=8:2,pH=8)溶液中没有荧光,H2S与其发生硫解反应释放出具有荧光... 详细信息

硫化氢(H2S)不仅参与人体生理调节,也对酒的风味和口感有影响.合成了一种2,4-二硝基苯修饰的苯并吡喃衍生物(DCM-dip),该化合物在二甲基亚砜(DMSO)/磷酸缓冲溶液(PBS)(V:V=8:2,pH=8)溶液中没有荧光,H2S与其发生硫解反应释放出具有荧光的中间体,进而实现荧光点亮识别***-dip识别H2S的响应时间为20 min,检测限为2.82μmol/l,pH适用范围为8~11,且具有较大的斯托克斯位移(123 nm),避免了背景干扰.应用研究表明,DCM-dip可用于红酒、啤酒样品中检测H2S,可对活细胞中H2S进行荧光成像.

关键词：苯并吡喃点亮型硫化氢荧光探针食品样品

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萘酚-苯并噻唑衍生物的合成及其琼脂糖凝胶对三文鱼新鲜度双通道无损检测

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食品科学 2024年第9期45卷 226-231页

作者：赵亚菲姚远钟克利孙小飞李学鹏汤立军励建荣渤海大学化学与材料工程学院辽宁锦州121013 渤海大学海洋研究院辽宁锦州121013 渤海大学食品科学与工程学院辽宁锦州121013 生鲜农产品贮藏加工及安全控制技术国家地方联合工程研究中心辽宁锦州121013

为了弥补单一比色法颜色区分不明显导致对鱼肉新鲜度的误判,本研究以萘酚为荧光团,通过与苯并噻唑盐缩合,合成萘酚-苯并噻唑衍生物(naphthol-benzothiazole derivative,HBO),其能够对有机胺比色和荧光双响应识别,并应用于三文鱼肉新鲜... 详细信息

为了弥补单一比色法颜色区分不明显导致对鱼肉新鲜度的误判,本研究以萘酚为荧光团,通过与苯并噻唑盐缩合,合成萘酚-苯并噻唑衍生物(naphthol-benzothiazole derivative,HBO),其能够对有机胺比色和荧光双响应识别,并应用于三文鱼肉新鲜度的无损检测。HBO在EtOH/H2O(1∶9,V/V)体系中对12种胺都能产生明显响应,具有较低的检测限(精胺:0.4μmol/l),并可在活细胞中对精胺荧光成像。以琼脂糖为基质,将探针负载后制备传感凝胶HBO琼脂糖凝胶(HBO agarose gel,HBOAl),当凝胶与三文鱼鱼肉共同贮藏时,HBOAl日光下为浅红色,紫外光照射为黄色荧光时表明鱼肉已腐败。该凝胶对三文鱼鱼肉的3种等级颜色区分明显,评价结果准确,可用于实时监测三文鱼鱼肉的新鲜度。

关键词：传感凝胶总挥发性盐基氮三文鱼新鲜度无损检测

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负载荧光探针的指示标签对三文鱼新鲜度的实时可视化监测

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中国食品学报 2024年第4期24卷 327-335页

作者：孙小飞李娟宫慧慧王增东钟克利汤立军李学鹏励建荣渤海大学食品科学与工程学院化学与材料工程学院海洋研究院生鲜农产品贮藏加工及安全控制技术国家地方联合工程研究中心辽宁锦州121013 山东安源种业科技有限公司山东烟台265617 中国科学院大连化学物理研究所分子反应动力学国家重点实验室辽宁大连116023

实时可视化监测鱼肉新鲜度,对于保障食品质量和消费者安全至关重要。以二甲氨基肉桂醛和异弗尔酮丙二腈为原料,合成一种具有供体(D)-π-受体(A)结构的荧光探针CDI,用来实时监测三文鱼肉的新鲜度。此探针对13种胺类物质响应明显,且对水... 详细信息

实时可视化监测鱼肉新鲜度,对于保障食品质量和消费者安全至关重要。以二甲氨基肉桂醛和异弗尔酮丙二腈为原料,合成一种具有供体(D)-π-受体(A)结构的荧光探针CDI,用来实时监测三文鱼肉的新鲜度。此探针对13种胺类物质响应明显,且对水产品腐败过程中产生的尸胺的荧光检测限低至9.91μmol/l。采用物理沉降法将探针CDI负载到纤维滤纸上,再加入盐酸使其质子化,得到负载探针CDI+H~+的指示标签。将指示标签置于三文鱼肉上空时,随着三文鱼肉新鲜度的下降,指示标签从白色变为浅黄色直至黄色,荧光逐渐开启,从紫粉色变为橙红色。通过对照总挥发性盐基氮值,验证了负载探针CDI+H^(+)的指示标签可以通过比色荧光双响应信号很好地区分三文鱼肉的3个新鲜度等级。

关键词：荧光探针指示标签三文鱼新鲜度

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选择不同对照疫苗对疑似预防接种异常反应信号检测的影响

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中国药物警戒 2024年第7期21卷 798-804,810页

作者：夏兰芳李克莉王磊李燕王华庆中国疾病预防控制中心免疫规划中心北京100050 传染病溯源预警与智能决策全国重点实验室北京100050

目的探索不同类型疫苗做对照时的信号检出情况,为疑似预防接种异常反应(AEFI)信号检测中选择适宜对照疫苗提供参考。方法分别选择所有其他疫苗和同技术路线其他疫苗做对照,对所有疫苗和特定疫苗进行信号检测,汇总所有其他疫苗和同技术... 详细信息

目的探索不同类型疫苗做对照时的信号检出情况,为疑似预防接种异常反应(AEFI)信号检测中选择适宜对照疫苗提供参考。方法分别选择所有其他疫苗和同技术路线其他疫苗做对照,对所有疫苗和特定疫苗进行信号检测,汇总所有其他疫苗和同技术路线其他疫苗做对照时信号检出数量,相同信号、新出现信号和消失信号个数及其反应分类分布,检测灵敏度和特异度。结果与所有其他疫苗做对照时相比,同技术路线其他疫苗做对照时,各种类疫苗信号检出个数均明显减少,全部信号检出数从501个减少至254个;新出现信号35个,新出现信号比例最大的是多糖疫苗,为24.39%(10/41),反应分类为一般反应(10个)、异常反应(3个)、偶合症(2个);消失信号282个,消失信号比例最大的是重组蛋白疫苗,为94.92%(56/59),消失信号的反应分类为偶合症(53个)、异常反应(23个)、一般反应(13个)。同技术路线其他疫苗做对照时,检测一般反应和异常反应的灵敏度降低,特异度增加,但减毒活疫苗和多糖疫苗中检测一般反应的灵敏度增加。结论不同类型疫苗做对照时,检出信号数差异较大;同技术路线其他疫苗做对照时,可以增加一般反应中信号的检出数量,但可能会造成较多异常反应信号消失。建议对于较严重的异常反应进行检测时,使用所有其他疫苗做对照。

关键词：疑似预防接种异常反应疫苗安全信号检测对照灵敏度特异技术路线

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Effect of combining biogeotextile and vegetation cover on the protection of steep slope of highway in northern China: A runoff plot experiment

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International Journal of Sediment Research 2023年第3期38卷 387-395页

作者： Hongyuan liu liang liu keli Zhang Ren Geng Key laboratory of Wetland Ecology and Environment Northeast Institute of Geography and Agroecology Chinese Academy of Sciences Co-Innovation Center for Sustainable Forestry in Southern China of Jiangsu Province Key Laboratory of Soil and Water Conservation and Ecological Restoration of Jiangsu Province Nanjing Forestry University School of Geography Faculty of Geographical Science Beijing Normal University State Key laboratory of Hydrology-Water Resources and Hydraulic Engineering Nanjing Hydraulic Research Institute

Biogeotextiles can be used to facilitate the formation of vegetation cover and to reduce soil *** have demonstrated that only biogeotextile or vegetation cover can greatly reduce soil ***, information about the effects of the combination of biogeotextile and vegetation cover on soil erosion is still limited, despite that the combination is the commonly practical form for bare road slope protection. Experimental plots, consisting of a relatively loose surface layer and a compacted sublayer,were constructed using movable soil-bin trolleys(200 × 100 × 40 cm3) filled with silt loam sieved through 1-cm screen. The plots were seeded with a seed mixture of tall fescue(Festuca elata Keng ex ***) and alfalfa(Medicago sativa l.) as a grass-legume regime. Then the plots were covered with three types of biogeotextile, i.e., coir blanket(CB), mixed coir-straw blanket(MB), and straw blanket(SB).A bare slope(BS) was constructed as the control experiment. Irrigation was applied to ensure vegetation germination and growth. Plant characteristics were measured after every 20 days. laboratory simulated rainfall of 71 mm/h intensity was applied to each plot for 60 min after 20, 40, and 60 days. Surface runoff and sediment were collected every 5 min during rainfall. The results showed that the MB and SB promoted increased total emergence density of plants by 47% and 23%, respectively, compared to case of vegetation growth without biogeotextiles. The dense structure of the CB impeded the emergence of alfalfa, leading to 9% lower total emergence density. Compared with bare slope(0 day BS), biogeotextiles increased runoff by 3%(MB)-19%(CB) and reduced erosion by 96%(MB and SB)-98%(CB). After 60 days,vegetation cover reduced runoff by 54% and reduced erosion by 81% compared with the BS case. The combination of biogeotextile and vegetation cover reduced runoff by 44%(CB)-62%(SB) and reduced erosion by no less than 99% compared to the BS case. Compared with biogeo

关键词： Biogeotextile Vegetation cover Slope protection Runoff control Erosion control

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Synthesis, isolation and characterization of methyl levulinate from cellulose catalyzed by extremely low concentration acid

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Journal of Energy Chemistry 2013年第6期22卷 895-901页

作者： Hui li lincai Peng lu lin keli Chen Heng Zhang Faculty of Chemical Engineering Kunming University of Science and Technology State Key laboratory of Pulp and Paper Engineering South China University of Technology School of Energy Research Xiamen University

A direct synthesis of methyl levulinate from cellulose alcoholysis in methanol medium under mild condition(180 210 C)catalyzed by extremely low concentration sulfuric acid(0.01 mol/l)and the product isolation were developed in this *** of different process variables towards the catalytic performance were performed as a function of reaction *** results indicated that sulfuric acid concentration,temperature and initial cellulose concentration had significant effects on the synthesis of methyl *** optimized yield of around 50%was achieved at 210 C for 120 min with sulfuric acid concentration of 0.01 mol/l and initial cellulose concentration below 100 g/*** resulting product mixture was isolated by a distillation technique that combines an atmospheric distillation with a vacuum distillation where n-dodecane was added to help distill the heavy *** light fraction including mainly methanol could be reused as the reaction medium without any substantial change in the yield of methyl *** chemical composition and structural of lower heavy fraction were characterized by GC/MS,FTIR,1H-NMR and13C-NMR *** levulinate was found to be a major ingredient of lower heavy fraction with the content over 96%.This pathway is efficient,environmentally benign and economical for the production of pure levulinate esters from cellulose.

关键词： cellulose methyl levulinate extremely low acid catalysis alcoholysis isolation

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Invasive sequence and ecological niche of main insect borers of larix gmelinii forest in Aershan, Inner Mongolia

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Forest Ecosystems 2008年第1期10卷 9-13页

作者： Kari keliVAARA Department of Applied Biology University of Helsinki FIN-00014 Finland

After the outbreak of Dendrolimus superans Buter in 2002, many insect borers quickly invaded larch (larix gmelinii Rupr.) forests in the Aershan of Inner Mongolia. Methods involved included setting sample plots, collecting adults in iron traps and measuring areas of galleries to study the invasive sequence, their ecological niche and the extent of the different effects by the main insect borers to their hosts. The results showed that the damage of D. superans weakened l. gmelinii, first Ips subelongatus Motschulsky invaded, followed by Acanthocinus carinulatus Gebler, Monochamus urussovi Fisher and M. sutor l. After the outbreak of D. superans, the average density of longhorn beetles per l. gmelinii tree increased. The ecological niche of Ips subelongatus stretches almost from the base to the top of the trunk. The number of insects in older stands of l. gmelinii is larger than those in middle aged stands. They do not damage healthy trees of l. gmelinii. The ecological niche of A. carinulatus is higher in dead l. gmelinii trees than in weak ones. The degree of damage is directly proportional with age and depth of bark. M. urussovi mainly damages trunks below 4 m in weak trees; in dead trees they can do damage up to 6 m in height. M. sutor mainly damages trunks below 5 m in weak l. gmelinii trees; in dead trees they cause damage up to 7 m. Again, the degree of damage is directly proportional with age. None of the three species of longhorn beetles damage healthy l. gmelinii and younger trees. Among the main insect borers, the degree of damage caused by I. subelongatus is more serious than that of other insects.

关键词： Dendrolimus superans invasive sequence insect borer ecological niche damage degree

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