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Chemical composition and Pb(II)binding of dissolved organic matter in a hypersaline lake in China

Journal of Oceanology and Limnology 2022年第1期40卷 113-127页

作者： keli yang Yaoling ZHANG Yaping DONG Wu LI Key Laboratory of Comprehensive and Highly Effi cient Utilization of Salt Lake Resources Qinghai Institute of Salt LakesChinese Academy of SciencesXining 810008China Qinghai Technology Research and Development Center of Comprehensive Utilization of Salt Lakes Resources Xining 810008China Qinghai Provincial Key Laboratory of Resources and Chemistry of Salt Lakes Xining 810008China

Dissolved organic matter(DOM)plays a vital role in promoting carbon and nutrient *** is a food source for organisms and controls the migration and transformation of trace metals and other contaminants in aquatic *** contributions of aquatic DOM to the environment and ecology of a system are closely related to its abundance and chemical *** this study,the chemical composition and binding properties of DOM in a hypersaline lake watershed were investigated for the fi rst time using dissolved organic carbon(DOC)analysis,absorption spectroscopy,Fourier transform infrared spectroscopy,pyrolysis-GC-MS(Py-GC-MS),and fl uorescence parallel factor(PARAFAC)analysis combined with Pb(II)titration *** results showed that DOM from the tributaries that fl owed into the lake had a lower DOC content,higher molecular weight,and higher specifi c UV absorbance than the DOM in lake ***-like fl uorophores were mainly found in tributary and lake surface water DOM(LSDOM)and humic-like substances were abundant in lake groundwater DOM(LGDOM).Using this multi-methodological approach,we found that the DOM from the hypersaline lake watershed was mainly from microbial origins,and consisted of aromatics,carbohydrates,and *** results from quantitative analysis showed that DOM from the infl owing tributaries contained more aromatics,lower carbohydrates,and lower aliphatics than DOM in the *** aromatic hydrocarbons and carbohydrates were more abundant in LSDOM than *** results from the Pb(II)titration technique coupled with PARAFAC analysis suggested that PARAFAC-derived components had relatively low condition stability constants(log K_(M)<2).Of the two types of lake DOM,the LGDOM had a higher Pb(II)binding potential than the *** this study we have improved our understanding of how DOM within a hypersaline lake watershed varies in its composition and potential to bind with metals.

关键词： dissolved organic matter hypersaline lake watershed composition parallel factor analysis Pb(II)titration

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Polyhydroxyalkanoates in tissue repair and regeneration

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Engineered Regeneration 2022年第1期3卷 24-40页

作者： Wentai Guo keli yang Xiusen Qin Rui Luo Hui Wang Rongkang Huang Department of Colorectal Surgery Guangdong Provincial Key Laboratory of Colorectal and Pelvic Floor DiseasesThe Sixth Affiliated Hospital of Sun Yat-sen UniversityGuangzhou 510655China Institute of Biomedical Innovation and Laboratory of Regenerative Medicine and Biomaterials Biomedical Material Conversion and Evaluation Engineering Technology Research Center of Guangdong ProvinceGuangzhou 510655China School of Materials Science and Engineering National Engineering Research Center for Tissue Restoration and ReconstructionSouth China University of TechnologyGuangzhou 510006China

Developing advanced biocompatibility materials is of critical importance in biomedical ***-yalkanoates(PHA),being famous for its flexible mechanical properties,thermal properties,biocompatibility,and biodegradability,has been widely used in wound dressings,artificial blood vessels,heart valves,nerve conduits,bone and cartilage scaffolds,surgical sutures,and other ***,reports on the application of PHA in tissue repair and regeneration are often lacking in ***,a comprehensive and in-depth perception of the performance advantages and application value of PHA is *** this review,the following applications of PHA in biomedical engineering are covered:i)soft tissue,ii)organ tissue,iii)vascular tissue,iv)heart valve tissue,v)nerve conduit tissue,vi)bone tissue,vii)cartilage tissue and viii)***,an outlook on the future research directions and challenges of PHA is presented.

关键词： Polyhydroxyalkanoates Biomedical engineering Tissue repair Tissue regeneration Biomaterial

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Prediction of Protein Structural Classes Using the Theory of Increment of Diversity and Support Vector Machine

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Wuhan University Journal of Natural Sciences 2011年第3期16卷 260-264页

作者： WANG Fangping WANG Zhijian LI Hong yang keli School of Physics and Information Science Tianshui Normal University Tianshui 741001Gansu China College of Sciences and Technology Inner Mongolia University Hohhot 010021 Inner Mongolia China

Based on the concept of the pseudo amino acid composition （PseAAC）, protein structural classes are predicted by using an approach of increment of diversity combined with support vector machine （ID-SVM）, in which the dipeptide amino acid composition of proteins is used as the source of diversity. Jackknife test shows that total prediction accuracy is 96.6% and higher than that given by other approaches. Besides, the specificity （Sp） and the Matthew＇s correlation coefficient （MCC） are also calculated for each protein structural class, the Sp is more than 88%, the MCC is higher than 92%, and the higher MCC and Sp imply that it is credible to use ID-SVM model predicting protein structural class. The results indicate that： 1 the choice of the source of diversity is reasonable, 2 the predictive performance of IDSVM is excellent, and3 the amino acid sequences of proteins contain information of protein structural classes.

关键词： dipeptide amino acid composition increment of diversity support vector machines protein structure classes

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Chemical characterization of fractions of dissolved humic substances from a marginal sea—a case from the Southern Yellow Sea

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Journal of Oceanology and Limnology 2018年第2期36卷 238-248页

作者： ZHANG Yaoling yang keli DU Jinzhou ZHANG Fenfen DONG Yaping LI Wu Qinghai Institute of Salt Lakes Chinese Academy of Sciences Xining 810008 China State Key Laboratory of Estuarine and Coastal Research East China Normal University Shanghai 200062 China

Marine dissolved organic matter （DOM） is one of the largest dynamic pools of organic carbon in the global carbon cycle, yet DOM is still chemically poorly characterized. To better understand the origin, composition, and cycling of DOM in the China marginal sea, dissolved humic substances （DHS） were isolated from seawaters in two locations in the Southern Yellow Sea. The DHS were subdivided into fulvic acids （FAs）, humic acids （HAs） and the XAD-4 fractions. Complementary analytical approaches were used to characterize the isolated DHS samples including stable carbon isotopic composition, Fourier transform infrared spectroscopy （FTIR）, ~3C cross polarization magic angle spinning （CP/MAS） nuclear magnetic resonance （NMR）, and pyrolysis gas chromatography-mass spectrometry （Py-GC/MS）. The results demonstrated that both DHS samples encountered the influences from marine source, indicating that algal and microbial-derived materials are the predominant precursors for the studied samples. The three fractions of DHS showed different properties. FAs presented more aromatic features, whereas HAs contained more aliphatic lipids and proteinaceous materials. The XAD-4 fractions were enriched in 13C and contained more carbohydrates but less aromatic compounds. The lower molecular weight and higher heteroatom content and number of carboxyl groups for the XAD-4 fractions may give them considerable geochemical significance for aspects of trace metal species, bioavailability of pollutants, mineral weathering and water acidification in marine environments.

关键词： seawater fulvic acid humic acid the XAD-4 fraction NMR Py-GC/MS

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Using benzene carboxylic acids to prepare zirconium-based catalysts for the conversion of biomass-derived furfural

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International Journal of Coal Science & Technology 2018年第4期5卷 464-472页

作者： Huacong Zhou Yufei Sha Zhenhuan Xiao Lu Li Jianmin Hao keli yang Na Li Runxia He Quansheng Liu College of Chemical Engineering Inner Mongolia University of Tech no logyInner Mongolia Key Laboratory of High-Value Functional Utilization of Low Rank Carbon ResourcesNo.49 Aimin Road Xincheng DistrictHuhhot 010051Inner MongoliaChina

Benzene carboxylic acid (BCAs) are common and useful chemical blocks, which can be derived from the abundant low rank coals (LRCs) via oxidative degradation. In this work, we proposed a novel strategy to utilize BCAs as raw materials to prepare catalysts with transition metal zirconium, and the prepared catalysts were applied into the conversion of the renewable biomass resources. Typical model BCAs in the oxidative products of LRCs, including pyromellitic acid, trimesic acid (TMSA), trimellitic acid, and benzoic acid, were used as the block to construct the Zr- BCAs catalysts. The chemoselective conversion of furfural into furfuryl alcohol (FAL). an important reaction in the biomass conversion chain, is chosen to evaluate the activity of the catalysts. The preparation conditions of the catalysts and experiment factors during the reaction were systematically investigated. The prepared catalysts were characterized by SEM, TEM, XRD and TG-DTG. The results showed that the prepared catalysts were efficient for the conversion of furfural into FAL, among which Zr-TMSA gave the highest activity. Zr-TMSA could be recycled for ten times without obvious deactivation, indicating an excellent stability. The strategy proposed in this work may be beneficial for the value-added utilization of both LRCs and biomass resources.

关键词： Benzene carboxylic acids Low rank coals Zirconium catalyst Biomass Furfural

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A direct real-time polymerase chain reaction assay for rapid high-throughput detection of highly pathogenic North American porcine reproductive and respiratory syndrome virus in China without RNA purification

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Journal of Animal Science and Biotechnology 2015年第1期6卷 22-28页

作者： Kang Kang keli yang Jiasheng Zhong Yongxiang Tian Limin Zhang Jianxin Zhai Li Zhang Changxu Song Christine Yuan Gou Jun Luo Deming Gou College of Animal Science and Technology Northwest A&F University College of Life Sciences Shenzhen Key Laboratory of Microbial Genetic EngineeringShenzhen University Hubei Key Laboratory of Animal Embryo and Molecular Breeding Institute of Animal Husbandry and VeterinaryHubei Academy of Agricultural Sciences Shenzhen Ao Dong Inspection and Testing Technology Co .Ltd Veterinary Medicine Institute Guangdong Academy of Agricultural Sciences Northwestern University

Background： Porcine reproductive and respiratory syndrome virus （PRRSV）, and particularly its highly pathogenic genotype （HP-PRRSV）, have caused massive economic losses to the global swine industry. Results： To rapidly identify HP-PRRSV, we developed a direct reaL-time reverse transcription polymerase chain reaction method （dRT-PCR） that could detect the virus from serum specimen without the need of RNA purification Our dRT-PCR assay can be completed in 1.5 h from when a sample is received to obtaining a result. Additionally, the sensitivity of dRT-PCR matched that of conventional reverse transcription PCR （cRT-PCR） that used purified RNA The lowest detection limit of HP-PRRSV was 6.3 TCIDs0 using dRT-PCR. We applied dRT-PCR assay to 144 field samples and the results showed strong consistency with those obtained by cRT-PCR. Moreover, the dRT-PCR method was able to tolerate 5-20% （v/v） serum. Conclusions： Our dRT-PCR assay allows for easier, faster, more cost-effective and higher throughput detection of HP-PRRSV compared with cRT-PCR methods. To the best of our knowledge, this is the first report to describe a real-time RT-PCR assay capable of detecting PRRSV in crude serum samples without the requirement for purifying RNA. We believe our approach has a great potential for application to other RNA viruses.

关键词： Highly pathogenic Porcine reproductive and respiratory syndrome virus Real-time RT-PCR

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Effect of Boron on Animal performance

Effect of Boron on Animal performance

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中国畜牧兽医学会动物解剖及组织胚胎学分会第十九次学术研讨会

作者： Haseeb Khaliq AR Ansari Juming Zhong Jing Wang keli yang Ke Xiao Kemei Peng College of Veterinary Medicine Huazhong Agricultural University

Boron is ubiquitous in nature,very indispensable for animal if proffer in specific *** are exposed to boron by water and different *** sources of boron exposure could be either through geological or via ground *** has been associated with persuading a variety of benefits in body organ systems:immune,bone,liver,CNS and *** this review,we outline the effects of boron on the animal *** growth performance and immune organ like thymus depict better immunity with boron *** metabolism also reveals good *** case of bone there is better Condition of animals fed boron diet,CNS also well *** a boron intake of approximately 1.0mg/d by consuming foods high in boron,like vegetables,nuts,pulses and fruits,,is a healthy dietary referral to improve *** boron is very helpful but the amount of boron should be up to certain limit because high level of boron might be show some toxic effects.

关键词： Boron Source Importance Toxicity

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Effects of Supplemental Boron on intestinal proliferation and apoptosis in African Ostrich Chicks

Effects of Supplemental Boron on intestinal proliferation an...

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中国畜牧兽医学会动物解剖及组织胚胎学分会第十九次学术研讨会

作者： Pengpeng Sun Haseeb Khaliq Abdur Rahman Ansari Jing Wang keli yang Ke Xiao Kemei Peng College of Veterinary Medicine Huazhong Agricultural University

Boron is an essential trace element which plays an important role in process of metabolism of many biological compounds and the function of the *** aim of this study was to investigate the effects of boron on the intestinal cell proliferation and apoptosis in African Ostrich chicks.A total of 36,ten day-old ostrich chicks were divided into six groups and fed on the same basal diet supplemented with 40,80,160,320 and 640 mg/L boric acid in drinking water for 80 *** cell nuclear antigen(PCNA) was used to test the proliferation index of intestine in different group by immunohistochemical staining(IHC).Apoptotic cells of intestine were detected by means of TUNEL and evaluated by mean optical *** showed that proliferation of intestinal cells significantly increased in high dose of boron groups(160,320 and640 mg/L).TUNEL reaction showed that apoptosis significantly decreased in 40 and 80 mg/L groups,while significantly increased in high dose of boron groups(320 and 640 mg/L),especially in *** conclusion,low dose of boron-supplemented water could promote growth of intestinal villus by depressing apoptosis,while high dose of boron could cause intestinal apoptosis and thus we found increased proliferation of intestine cell as a compensatory *** findings may support optimal dosage of boron that could protect the development of Ostrich intestine,while high dosage of boron could suppress it,or even has toxic effects on it.

关键词： Apoptosis Boron Ostrich chicks Intestine PCNA

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非局域耦合帐篷映像中的完全同步周期态

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陕西师范大学学报（自然科学版） 2015年第3期43卷 26-30页

作者：杨科利屈世显陕西师范大学物理学与信息技术学院陕西西安710119 宝鸡文理学院物理与光电技术学院陕西宝鸡721016

研究了非局域耦合帐篷映像中的周期态,通过计算耦合系统的信息熵和同步序参量,发现系统中存在两类周期态,即非同步周期态和完全同步周期态。非同步周期态在空间呈现连续的波型分布,时间上也呈现周期性。其空间周期随着耦合半径的减小而... 详细信息

研究了非局域耦合帐篷映像中的周期态,通过计算耦合系统的信息熵和同步序参量,发现系统中存在两类周期态,即非同步周期态和完全同步周期态。非同步周期态在空间呈现连续的波型分布,时间上也呈现周期性。其空间周期随着耦合半径的减小而减小,时间周期随系统参数的改变而变化。当完全同步态出现时,系统中各格点的动力学状态同步到单映像的非稳定周期态,这与以往的耦合系统中系统同步于单映像的稳定周期轨道有所不同。这类同步态的出现归因于格点间的动力学耦合,可以模拟神经元间触突耦合的丰富放电行为。

关键词：周期态信息熵同步帐篷映像

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永磁同步直线电机的趋近率自适应软切换离散滑模控制

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机床与液压 2018年第4期46卷 120-123页

作者：杨克立程辉中原工学院工业训练中心河南郑州450007 河南工程学院电气信息工程学院河南郑州451192

针对存在不确定参数、摩擦及外部扰动的永磁同步直线电机伺服系统,提出一种自适应软切换滑模位置控制策略。首先,将直线电机伺服系统的模型进行状态离散化;然后根据离散化的系统模型,设计一种切换增益自适应变化的滑模控制器;同时,对设... 详细信息

针对存在不确定参数、摩擦及外部扰动的永磁同步直线电机伺服系统,提出一种自适应软切换滑模位置控制策略。首先,将直线电机伺服系统的模型进行状态离散化;然后根据离散化的系统模型,设计一种切换增益自适应变化的滑模控制器;同时,对设计的闭环控制系统进行稳定性分析,并利用正切函数代替符号函数,进一步改善系统的性能;最后通过仿真实验将此方案与经典PID控制及滑模控制进行比较,仿真结果验证了此方案具有更好的跟踪性能和对不确定扰动的鲁棒性。

关键词：直线电机离散滑模控制趋近率自适应

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