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Determination of the archaeal and bacterial communities in two-phase and single-stage anaerobic systems by 454 pyrosequencing

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Journal of Environmental Sciences 2015年第10期27卷 121-129页

作者： Yogananda Maspolim Yan Zhou Chenghong Guo keke xiao Wun Jern Ng Advanced Environmental Biotechnology Centre (AEBC) Nanyang Environment and Water Research Institute (NEWRI)Nanyang Technological University (NTU) School of Civil and Environmental Engineering Nanyang Technological University (NTU)

2-Phase anaerobic digestion（AD）, where the acidogenic phase was operated at 2 day hydraulic retention time（HRT） and the methanogenic phase at 10 days HRT, had been evaluated to determine if it could provide higher organic reduction and methane production than the conventional single-stage AD（also operated at 12 days HRT）. 454 pyrosequencing was performed to determine and compare the microbial communities. The acidogenic reactor of the 2-phase system yielded a unique bacterial community of the lowest richness and diversity, while bacterial profiles of the methanogenic reactor closely followed the single-stage reactor. All reactors were predominated by hydrogenotrophic methanogens, mainly Methanolinea. Unusually, the acidogenic reactor contributed up to 24%of total methane production in the 2-phase system. This could be explained by the presence of Methanosarcina and Methanobrevibacter, and their activities could also help regulate reactor alkalinity during high loading conditions through carbon dioxide production. The enrichment of hydrolytic and acidogenic Porphyromonadaceae, Prevotellaceae, Ruminococcaceae and unclassified Bacteroidetes in the acidogenic reactor would have contributed to the improved sludge volatile solids degradation, and ultimately the overall 2-phase system＇s performance. Syntrophic acetogenic microorganisms were absent in the acidogenic reactor but present in the downstream methanogenic reactor, indicating the retention of various metabolic pathways also found in a single-stage system. The determination of key microorganisms further expands our understanding of the complex biological functions in AD process.

关键词： Sewage sludge 2-Phase anaerobic digestion Microbial community 454 pyrosequencing Methanogen

Insights into the changes of amino acids,microbial community,and enzymatic activities related with the nutrient quality of product during the composting of food waste

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Frontiers of Environmental Science & Engineering 2023年第3期17卷 93-105页

作者： Ying Xue keke xiao Xiang Wu Mei Sun Yifei Liu Bei Ou Jiakuan Yang School of Environmental Science and Engineering Huazhong University of Science and TechnologyWuhan 430074China

This study systematically investigated the changes of amino acids as the composting process of food waste *** is found that the addition of 0.3 g/g total solids of food waste achieved the highest seed germination index of the product(268%).The microbial community results indicated that the abundance of amino acid metabolism sequences remained at high levels during the whole composting *** was identified as the key amino acid related with the nutrient quality of product during the composting of food *** plant germination and hydroponic experiments found,that compared with those without the addition of proline,the addition of 50 mg/L proline increased seed germination rate by 20%,increased shoot length by 3%,increased root biomass of seedlings by 82%,and increased leaf biomass of seedlings by 76%,***,γ-Pseudomonadota,Chloroflexi and Planctomycetes were the key identified bacteria related with the increase of proline during the composting of food ***,the enzymatic tests of the activities of superoxide dismutase,peroxidase and malondialdehyde indicated that proline did not cause oxidative damage on the growth of *** study provided novel insights into the changes of amino acids,microbial community,and enzymatic activities related with the nutrient quality of product during the composting of food waste.

关键词： Composting Food waste Amino acids Microbial community Enzymatic activity Nutrient quality

Recent Advances on the Development of Functional Materials in Microbial Fuel Cells:From Fundamentals to Challenges and Outlooks

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Energy & Environmental Materials 2022年第2期5卷 401-426页

作者： Qian Zhu Jingping Hu Bingchuan Liu Shaogang Hu Sha Liang keke xiao Jiakuan Yang Huijie Hou School of Environmental Science and Engineering Huazhong University of Science and TechnologyWuhan Hubei 430074China Hubei Provincial Engineering Laboratory of Solid Waste Treatment Disposal and RecyclingWuhan Hubei 430074China State Key Laboratory of Coal Combustion Huazhong University of Science and TechnologyWuhan Hubei 430074China

Microbial fuel cells(MFCs),as a sustainable and promising technology to solve both environmental pollution and energy shortage,have captured tremendous *** conversion efficiency of chemical energy contained in organic waste or wastewater to electricity via microbial metabolism strongly depends on the performance of each functional unit,including the anode,cathode and separator/membrane used in ***,significant attention has been paid toward developing advanced functional materials to enhance the performance of each unit or provide new featured *** review paper provides a comprehensive review on recent achievements and advances in the modification and development of functional materials for MFC systems,including 1)the development of functional anode materials for enhanced microbial compatibilities as well as electron transfer capabilities,2)the development of cost-effective separators/membranes such as ion exchange membrane,porous membrane,polymer electrolyte membrane and composite membrane,and 3)the development of functional cathode catalysts to decrease the over-potential and enhance the electrocatalytic efficiency for oxygen reduction reaction in order to substitute the common costly Pt *** challenges and outlooks of functional materials for MFC applications are also discussed.

关键词： anode cathode functional material membrane microbial fuel cell

Comparison of different valent iron on anaerobic sludge digestion:Focusing on oxidation reduction potential,dissolved organic nitrogen and microbial community

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Frontiers of Environmental Science & Engineering 2022年第6期16卷 159-170页

作者： Zecong Yu keke xiao Yuwei Zhu Mei Sun Sha Liang Jingping Hu Huijie Hou Bingchuan Liu Jiakuan Yang School of Environmental Science and Engineering Huazhong University of Science and TechnologyWuhan 430074China Hubei Provincial Engineering Laboratory of Solid Waste Treatment Disposal and RecyclingWuhan 430074China State Key Laboratory of Coal Combustion Huazhong University of Science and TechnologyWuhan 430074China

This study compared effects of three different valent iron(Fe^(0),Fe(II)and Fe(III))on enhanced anaerobic sludge digestion,focusing on the changes of oxidation reduction potential(ORP),dissolved organic nitrogen(DON),and microbial *** the same iron dose in range of 0−160 mg/L after an incubation period of 30 days(d),the maximum methane production rate of sludge samples dosed with respective Fe^(0),Fe(II)and Fe(III)at the same concentration showed indiscernible differences at each iron dose,regardless of the different iron ***,their behavior in changes of ORP,DON and microbial community was different:(1)the addition of Fe^(0) made the ORP of sludge more negative,and the addition of Fe(II)and Fe(III)made the ORP of sludge less ***,whether being more or less negative,the changes of ORP may show unobservable effects on methane yield when it ranged from−278.71 to−379.80 mV;(2)the degradation of dissolved organic nitrogen,particularly proteins,was less efficient in sludge samples dosed with Fe^(0) compared with those dosed with Fe(II)and Fe(III)after an incubation period of 30 *** the same dose of 160 mg/L iron,more cysteine was noted in sludge samples dosed with Fe(II)(30.74 mg/L)and Fe(III)(27.92 mg/L)compared with that dosed with Fe^(0)(21.75 mg/L);(3)Fe^(0) particularly promoted the enrichment of Geobacter,and it was 6 times higher than those in sludge samples dosed with Fe(II)and Fe(III)at the same dose of 160 mg/L iron.

关键词： Enhanced anaerobic sludge digestion Different iron valence Oxidation reduction potential Dissolved organic nitrogen Microbial community

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勘测项目生产管理信息系统设计与实现

城市勘测 2024年第4期 20-22,26页

作者：肖可可何涛涛周翔陈旭帅温玉维郭义中国能源建设集团湖南省电力设计院有限公司湖南长沙410007

针对勘测项目管理过程中存在的问题,结合日常管理业务工作流程,分析系统建设需求,详细阐述了系统的总体架构、数据库设计、功能设计和安全设计,并在此基础上开发了适用于企业勘测项目生产管理的信息系统,提升勘测项目管理的信息化水平... 详细信息

针对勘测项目管理过程中存在的问题,结合日常管理业务工作流程,分析系统建设需求,详细阐述了系统的总体架构、数据库设计、功能设计和安全设计,并在此基础上开发了适用于企业勘测项目生产管理的信息系统,提升勘测项目管理的信息化水平和能力,深化精细管理,切实提高生产效率和管理水平,促进企业可持续性发展。

关键词：勘测项目生产管理 GIS 开源框架

Females adopt sexual catalepsy to facilitate mating

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Current Zoology 2024年第2期70卷 174-181页

作者： Jihe Liu keke Liu Yu Tang Weihua Wang Xiang Xu Jianhui Liang Yonghong xiao Mark A.Elgar College of Life Sciences Jinggangshan UniversityJi'anJiangxi 343009China Pharmaceutical Center Tsinghua UniversityBeijing 100084China College of Life Sciences Hunan Normal UniversityChangshaHunan 410081China School of Pharmaceutical Sciences Peking UniversityBeijing 100083China School of BioSciences University of MelbourneMelbourneVictoria 3010Australia School of Life Sciences and Technology Tongii UniversityShanghai 200092China

Theory predicts that males and females of dioecious species typically engage in an evolutionary sexual conflict over the frequency and choice of mating *** sexual cannibalism,a particularly dramatic illustration of this conflict,is widespread in certain animal taxa including ***,females of some funnel weaving spiders that are generally aggressive to conspecifics enter a cataleptic state after male courtship,ensuring the males can mate without risk of *** this study,we demonstrated that the physical posture and duration,metabo-lites,and central neurotransmitters of females of Aterigena aculeata in sexual catalepsy closely resemble females in thanatosis but are distinct from those in anesthesia,indicating that the courted females feign death to eliminate the risk of potentially aggressive responses and thereby allow preferred males to *** the taxonomically widespread thanatosis,which generally represents a deceptive visual signal that acts against the interest of the receivers,sexual catalepsy of females in the funnel weaving spiders may deliver a sexual-receptive signal to the courting males and thereby benefit both the signal senders and ***,sexual catalepsy in *** may not reflect a conflict butrather a confluence of interest betweenthe sexes.

关键词： female catalepsy funnel weaving spiders mate choice mating strategy sexual behavior.

Anaerobic digestion of sludge by different pretreatments:Changes of amino acids and microbial community

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Frontiers of Environmental Science & Engineering 2022年第2期16卷 109-123页

作者： keke xiao Zecong Yu Kangyue Pei Mei Sun Yuwei Zhu Sha Liang Huijie Hou Bingchuan Liu Jingping Hu Jiakuan Yang School of Environmental Science and Engineering Huazhong University of Science and TechnologyWuhan 430074China Hubei Provincial Engineering Laboratory of Solid Waste Treatment Disposal and Recycle TechnologyWuhan 430074China State Key Laboratory of Coal Combustion Huazhong University of Science and TechnologyWuhan 430074China

Many studies have investigated the effects of different pretreatments on the performance of anaerobic digestion of ***,the detailed changes of dissolved organic nitrogen,particularly the release behavior of proteins and the byproducts of protein hydrolysis-amino acids,are rarely known during anaerobic digestion of sludge by different *** we quantified the changes of three types of proteins and 17 types of amino acids in sludge samples solubilized by ultrasonic,thermal,and acid/alkaline pretreatments and their transformation during anaerobic digestion of *** protein,aromatic protein I,aromatic protein II,and cysteine were identified as the key dissolved organic nitrogen responsible for methane production during anaerobic digestion of sludge,regardless of the different pretreatment *** from the depletion of other amino acids,cysteine was resistant to degradation after an incubation period of 30 days in all sludge ***,the“cysteine and methionine metabolism(K00270)”was absent in all sludge samples by identifying 6755 Kyoto Encyclopedia of Genes and Genomes assignments of genes *** contributed to the generation of methane and the degradation of acetic,propionic,and n-butyric acids through decreasing oxidation-reduction potential and enhancing biomass *** study provided an alternative strategy to enhance anaerobic digestion of sludge through in situ production of cysteine.

关键词： Sludge pretreatments Dissolved organic nitrogen Proteins Amino acids Structural equation model Metagenomic sequencing analysis

添加秸秆堆肥处理厨余垃圾过程中蛋白类物质转化及微生物群落研究

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华中科技大学学报（自然科学版） 2022年第10期50卷 83-96页

作者：欧蓓薛映肖可可杨家宽陈海滨关文义程谦勋华中科技大学环境科学与工程学院湖北武汉430074 安徽国祯环卫科技有限公司安徽合肥230000

将玉米、水稻、油菜和小麦四种农业秸秆作为填充剂与厨余垃圾进行堆肥,分析堆体理化性质,利用不同表征方式分析堆体成熟度、稳定度变化情况,并采用高通量测序技术研究堆肥不同阶段的微生物菌群特性.结果表明:依照堆肥成熟要求,经35 d堆... 详细信息

将玉米、水稻、油菜和小麦四种农业秸秆作为填充剂与厨余垃圾进行堆肥,分析堆体理化性质,利用不同表征方式分析堆体成熟度、稳定度变化情况,并采用高通量测序技术研究堆肥不同阶段的微生物菌群特性.结果表明:依照堆肥成熟要求,经35 d堆肥后,仅未添加填充剂的堆肥处理组不满足堆肥成熟要求,而四组不同填充剂的堆肥产品均满足堆肥成熟要求;添加填充剂可以促进降解纤维素的变形菌门和拟杆菌门的富集;当堆肥过程结束时,4个秸秆组存在纤维弧菌和糖单孢菌等具有分解纤维素等难溶性有机质能力的菌属;与堆肥初期相比,添加玉米秸秆后,腐熟期氨基酸代谢序列的相对丰度增加了8.9%,且脯氨酸含量增加量最高,腐熟期时增加至129.69 mmol/kg.相较而言,玉米秸秆是较优的填充剂.

关键词：厨余垃圾好氧堆肥秸秆填充剂蛋白质氨基酸微生物群落

Structure-based design of pan-coronavirus inhibitors targeting host cathepsin L and calpain-1

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Signal Transduction and Targeted Therapy 2024年第4期9卷 1651-1664页

作者： Xiong Xie Qiaoshuai Lan Jinyi Zhao Sulin Zhang Lu Liu Yumin Zhang Wei Xu Maolin Shao Jingjing Peng Shuai Xia Yan Zhu keke Zhang Xianglei Zhang Ruxue Zhang jian Li Wenhao Dai Zhen Ge Shulei Hu Changyue Yu Jiang Wang Dakota Ma Mingyue Zheng Haitao Yang Gengfu xiao Zihe Rao Lu Lu Leike Zhang Fang Bai Yao Zhao Shibo Jiang Hong Liu Drug Discovery and Design Center State Key Laboratory of Drug ResearchCAS Key Laboratory of Receptor ResearchShanghai Institute of Materia MedicaChinese Academy of SciencesShanghai 201203China University of Chinese Academy of Sciences Beijing 100049China Key Laboratory of Medical Molecular Virology(MOE/NHC/CAMS) School of Basic Medical SciencesShanghai Institute of Infectious Disease and BiosecurityFudan UniversityShanghai 200032China Shanghai Institute for Advanced Immunochemical Studies and School of Life Science and Technology ShanghaiTech UniversityShanghai 201210China State Key Laboratory of Virology Wuhan Institute of VirologyCenter for Biosafety Mega-ScienceChinese Academy of SciencesWuhan 430071China School of Chinese Materia Medica Nanjing University of Chinese Medicine138 Xian Lin RoadJiangsu 210023 NanjingChina School of Pharmaceutical Science and Technology Hangzhou Institute for Advanced StudyUCASHangzhou 310024China National Clinical Research Center for Infectious Disease Shenzhen Third People's HospitalShenzhen 518112China

Respiratory disease caused by coronavirus infection remains a global health *** several SARS-CoV-2-specific vaccines and direct-acting antivirals are available,their effcacy on emerging coronaviruses in the future,including SARS-CoV-2 variants,might be ***-targeting antivirals provide preventive and therapeutic strategies to overcome resistance and manage future outbreak of emerging *** L(CTSL)and calpain-1(CAPN1)are host cysteine proteases which play crucial roles in coronaviral entrance into cells and infection-related immune ***,two peptidomimetic a-ketoamide compounds,14a and 14b,were identified as potent dual target inhibitors against CTSL and *** X-ray crystal structures of human CTSL and CAPN1 in complex with 14a and 14b revealed the covalent binding of a-ketoamide groups of 14a and 14b to C25 of CTSL and C115 of *** showed potent and broad-spectrum anticoronaviral activities in vitro,and it is worth noting that they exhibited low nanomolar potency against SARS-CoV-2 and its variants of concern(VOCs)with ECso values ranging from 0.80 to 161.7 nM in various *** mechanistic exploration indicated that they exhibited anticoronaviral activity through blocking viral ***,14a and 14b exhibited good oral pharmacokinetic properties in mice,rats and dogs,and favorable safety in *** addition,both 14a and 14b treatments demonstrated potent antiviral potency against SARS-CoV-2 XBB 1.16 variant infection in a K18-hACE2 transgenic mouse *** 14b also showed effective antiviral activity against HCoV-OC43 infection in a mouse model with a final survival rate of 60%.Further evaluation showed that 14a and 14b exhibited excellent antiinflammatory effects in Raw 264.7 mouse macrophages and in mice with acute *** together,these results suggested that 14a and 14b are promising drug candidates,providing novel insight into developing pan-coronavirus inhibitors with anti

关键词： calpain overcome entrance