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Hybrid Rubisco with Complete Replacement of Rice Rubisco Small Subunits by Sorghum Counterparts Confers C4 Plant-like High Catalytic Activity

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Molecular Plant 2020年第11期13卷 1570-1581页

作者： Hiroyoshi Matsumura keita shiomi Akito Yamamoto Yuri Taketani Noriyuki Kobayashi Takuya Yoshizawa Shun-ichi Tanaka Hiroki Yoshikawa Masaki Endo Hiroshi Fukayama Department of Biotechnology College of Life SciencesRitsumeikan University1-1-1 Noji-HigashiKusatsu 525-8577Japan Graduate School of Agricultural Science Kobe University1-1 Rokkodai-tyouNada-kuKobe 657-8501Japan Faculty of Agriculture Kobe University1-1 Rokkodai-tyouNada-kuKobe 657-8501Japan Division of Applied Genetics Institute of Agrobiological SciencesNational Agriculture and Food Research Organization1-2 OwashiTsukuba 305-8634Japan Department of Biomolecular Chemistry Kyoto Prefectural UniversityHangi-choShimogamoSakyo-kuKyoto 606-8522Japan

Photosynthetic rate at the present atmospheric condition is limited by the CO2-fixing enzyme ribulose-1,5-bisphosphate carboxylase/oxygenase(Rubisco)because of its extremely low catalytic rate(kcat)and poor affinity for C02(Kc)and specificity for CO2(Sc/O).Rubisco in C4 plants generally shows higher k cat than that in C3 *** consists of eight large subunits and eight small subunits(RbcS).Previously,the chimeric incorporation of sorghum C4-type RbcS significantly increased the k cat of Rubisco in a C3 plant,*** this study,we knocked out rice RbcS multigene family using the CRISPR-Cas9 technology and completely replaced rice RbcS with sorghum RbcS in rice *** hybrid Rubisco showed almost C4 plant-like catalytic properties,i.e.,higher kcai,higher Kc,and lower Sc/*** lines expressing the hybrid Rubisco accumulated reduced levels of Rubisco,whereas they showed slightly but significantly higher photosynthetic capacity and similar biomass production under high C 02 condition compared with wild-type ***-resolution crystal structural analysis of the wild-type Rubisco and hybrid Rubisco revealed the structural differences around the central pore of Rubisco and the pC-pD hairpin in *** propose that such differences,particularly in the pC-pD hairpin,may impact the flexibility of Rubisco catalytic site and change its catalytic properties.

关键词： CRISPR-Cas9 C4 plant photosynthesis rice Rubisco

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用于旋翼飞机开发研究的多分野解析框架

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Transactions of Nanjing University of Aeronautics and Astronautics 2024年第1期41卷 43-52页

作者： TANABE Yasutada SUGAWARA Hideaki KIMURA keita 日本宇宙航空研究开发机构航空技术部日本东京181-0015

介绍了日本宇宙航空研究开发机构研发的用于旋翼飞机的多分野解析框架。Heli Design是在各种旋翼飞机初期概念设计时使用的软件;rFlight可以提供在各种飞行条件下的平衡解析和线性化飞行模型;rBET/RMT是基于叶片要素理论的低保真度空力... 详细信息

介绍了日本宇宙航空研究开发机构研发的用于旋翼飞机的多分野解析框架。Heli Design是在各种旋翼飞机初期概念设计时使用的软件;rFlight可以提供在各种飞行条件下的平衡解析和线性化飞行模型;rBET/RMT是基于叶片要素理论的低保真度空力解析软件,和rMode提供的结构模型结合,可以推测旋转翼的弹性变形和各种飞行条件下的空力负载;rFlow3D是基于CFD计算的高保真度数值模拟软件;rGrid系列自动网格构造工具为其提供简单的网格生成;基于rFlow3D的计算结果,r Noise可以预测在任何观测点的噪声。这些软件已经广泛地被应用在各种新型旋翼飞机的开发研究中,其计算精度也已被各种实验结果所应证。

关键词：旋翼飞机多分野解析高保真模拟设计工具

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Two-phase flow thermo-hydro-mechanical modeling for a water flooding field case

Rock Mechanics Bulletin

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Rock Mechanics Bulletin 2024年第3期3卷 81-90页

作者： Yuhao Liu Fengshou Zhang Dingwei Weng Hongbo Liang Chunming He keita Yoshioka Key Laboratory of Geotechnical&Underground Engineering of Ministry of Education Tongji UniversityShanghai200092China Department of Geotechnical Engineering College of Civil EngineeringTongji UniversityShanghai200092China PetroChina Research Institute of Petroleum Exploration&Development Beijing100083China CNPC Key Laboratory of Oil&Gas Reservoir Stimulation Langfang065007China Department of Petroleum Engineering Montanuniversitat LeobenLeobenLeoben8700Austria

Simulation of subsurface energy system involves multi-physical processes such as thermal,hydraulical,and mechanical(THM)processes,and requires a so-called THM coupled modeling *** coupled modeling is commonly performed in geothermal energy ***,for hydrocarbon extraction,we need to consider multiphase flow *** this paper,we describe a three-dimensional numerical model of non-isothermal two-phase flow in the deformable porous medium by integrating governing equations of two-phase mixture in the porous media flow in the *** account for inter-woven impacts in subsurface condi-tions,we introduced a temperature-dependent fluid viscosity and a fluid density along with a strain-dependent reservoir ***,we performed numerical experiments of a ten-year water flooding pro-cess employing the open-source parallelized code,*** considered different well patterns with colder water injection in realistic *** results demonstrate that our model can simulate complex interactions of temperature,pore pressure,subsurface stress and water saturation simultaneously to evaluate the recovery *** temperature can promote fluid flow while cold water injection under non-isothermal conditions causes the normal stress reduction by significant thermal *** different well patterns the displacement efficiency will be changed by the relative location between injection and production *** finding has provided the important reference for fluid flow and induced stress evolution during hydrocarbon exploitation under the environment of large reservoir depth and high temperature.

关键词： THM coupling Two phase flow OpenGeoSys Water flooding Field-scale model

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A Biogas Production Model from the Combination of Pig Manure and Cow Dung in N’Zérékoré City, Republic of Guine

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Energy and Power Engineering 2024年第9期16卷 293-312页

作者： Madeleine Kamano Oumar keita Ansoumane Sakouvogui Aboubacar Sangare Department of Physics Universit de NZrkor NZrkor Guinea Department of Hydrology Universit de NZrkor NZrkor Guinea Department of Energy Institut Suprieur de Technologie de Mamou Mamou Guinea Department of Environmental Engineering Université de NZérékoré NZérékoré Guinea

This present research work focuses on the valorization of pig droppings for production of biogas in mono digestion and co-digestion with proportions of cow dung from the urban commune of N’Zérékoré. It was carried out in December 2020 in the Physics laboratory of the University of N’Zérékoré. The anaerobic digestion process took 25 days in an almost constant ambient temperature of 25˚C. Five digesters were loaded on 12/06/2020, two of which with 1 kg of pig dung and 1 kg of cow dung both in mono-digestion. The 3 other digesters in co-digestion with different proportions of pig manure and cow dung. The substrate in each digester is diluted in 2 liters of water, with a proportion of (1/2). The main results obtained are: 1) the evolution of the temperature and pH during digestion process, 2) the average biogas productions 0.61 liters for (D1);1.20 liter for (D2);1.65 liter for (D3);1.51 liter for (D4) and 1.31 liter for (D5). The cumulative amounts of biogas are respectively: D1 (7.95 liters), D2 (15.60 liters), D3 (21.50 liters), D4 (19.65 liters) and D5 (17.05 liters). The total cumulative production is 81.75 liters at the end of the process. The originality of this research work is that the proposed model examines the relation between the daily biogas production and the variation of temperature, pH and pressure. The combustibility test showed the biogas produced during the first week was no combustible (contains less than 50% methane). Combustion started from the biogas produced from the 15th day and it is from the 20th day that a significant amount of stable yellow/blue flame was observed. The results of this study show the combination of pig manure and cow dung presents advantages for optimal biogas production.

关键词： Production Experimental Model Pig Manure Cow Dung Biogas N’Zérékoré Republic of Guinea

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Epidemiology and Histopathology of Cancers of the Upper Aerodigestive Tract in the ENT and Head and Neck Surgery Department of the Ignace Deen National Hospital

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International Journal of Otolaryngology and Head & Neck Surgery 2024年第4期13卷 259-274页

作者： Mamadou Mouctar Ramata Diallo Oumou Amadou Diallo Raphan Madi Kaba keita Ousmane Barry Abdoulaye keita Alpha Oumar Diallo Department of Otorhinolaryngology and Head and Neck Surgery Mamou Regional Hospital Mamou Guinea Faculty of Health Sciences and Techniques Gamal Abdel Nasser University of Conakry Conakry Guinea Department of Otorhinolaryngology and Head and Neck Surgery Ignace Deen National Hospital Conakry Guinea Department of Otorhinolaryngology and Head and Neck Surgery NZrkor Regional Hospital NZrkor Guinea Department of Otorhinolaryngology and Head and Neck Surgery Donka National Hospital Conakry Guinea

Introduction: Head and neck cancers represent a significant burden of cancer, collectively ranking fourth for cancer incidence and second for cancer mortality worldwide. Objective: To study the epidemiological and histopathological aspects of head and neck cancers in the ENT-CCF department of Ignace Deen National Hospital. Materials and Methods: This was a descriptive retrospective study covering a period of 5 years from September 1, 2016, to September 30, 2021. Results: We collected 43 cases of head and neck cancers, representing a hospital frequency of 1.85%. The mean age was 45.21 ± 13.23 years. Males predominated, accounting for 65.12%, compared to 34.88% for females, with a male-to-female ratio of 1.87. Housewives accounted for 27.90%, followed by farmers at 23.26% and traders/merchants at 18.60%, with 41.86% being uneducated. The main risk factors were tobacco (37.21%) and alcohol (34.88%). Pain (53.49%), dysphonia (51.16%), and dyspnea (39.53%) were the main presenting symptoms. Squamous cell carcinoma (74.42%) and large cell lymphoma (18.60%) were the most commonly cited histological types. Conclusion: Head and neck cancers remain relatively common in our country. Improving prognosis depends on early diagnosis of the condition, resuscitation resources, and prompt management.

关键词： Cancer Head and Neck Histopathology Ignace Deen National Hospital

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Literature Review on Support for Children and Families Experiencing Parental Bereavement

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Open Journal of Nursing 2024年第4期14卷 139-163页

作者： Rurie Namiki keita Sasaki Iku Taniguchi Rie Wakimizu Department of Nursing Science Graduate School of Comprehensive Human Sciences University of Tsukuba Ibaraki Japan Department of Child Health Care Nursing Division of Health Innovation and Nursing Faculty of Medicine University of Tsukuba Ibaraki Japan

Purpose: This study aimed to understand the actual nursing support in a wide perspective by reviewing overseas literature on support for children who have experienced parental bereavement and their families. The goal was to identify future challenges in nursing support in clinical practice in Japan. Method: Literature searchable as of May 2023 was retrieved using PubMed, resulting in 11 relevant articles. Result: The results revealed the following: 1) For support provided to children, 13 codes were condensed into 5 subcategories and 4 categories. 2) For support provided to families, 36 codes were condensed into 11 subcategories and 4 categories. Conclusion: Open communication was found to be essential for supporting children and their families who have experienced parental bereavement. Moreover, involvement of multiple professions facilitated the provision of specialized support to address diverse needs of children and families, playing a crucial role in overcoming grief. Additionally, the effectiveness of support systems for bereaved families highlighted the need for nursing professionals in Japan to gain knowledge through learning opportunities and to establish a multi-disciplinary approach to support, thus indicating future challenges in nursing support.

关键词： Terminal Care Family Support Child Parental Death Palliative Care

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The Management of Neonatal Peritonitis in the Paediatric Surgery Department of the Donka National Hospital

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Open Journal of Pediatrics 2024年第5期14卷 823-833页

作者： Mohamed Lamine Sadou Sacko Thierno Saidou Barry Balla keita Mory Sangare Jacques Faya Millimono Moussa Conde Seydou keita Daniel Agbo-Panzo Pediatric Surgery Department at Conakry University Hospital Conakry Guinea Universit Gamal Abdel Nasser de Conakry Conakry Guinea General Surgery Department Ignace Deen University Hospital Conakry Guinea

Introduction: Neonatal peritonitis is a rare but serious condition requiring early diagnosis and management. The lack of antenatal diagnosis, the precariousness of neonatal intensive care, and the delay in diagnosis and treatment are the factors of poor prognosis. The objective of this study was to study the epidemiological, diagnostic and therapeutic aspects of neonatal peritonitis in order to improve its management in the pediatric surgery department of the CHU-Donka. Materials and Methods: This is a retrospective descriptive study of the records of patients treated for neonatal peritonitis from January 1, 2017 to December 31, 2022. Results: Results: We compiled 17 records of patients operated on for neonatal peritonitis, representing a frequency of 3.85% and an incidence of 2.83 cases/year. The mean age of our patients was 8.29 days, with extremes of D0 and D25. Males were more dominant, with a frequency of 71% and a sex ratio of 2.4. The average consultation time was 72 h, with extremes of 12 h and 144 h. Abdominal bloating was the main reason for consultation (100%). Fever and vomiting were frequent, at 70.59% and 82.35% respectively. PSA revealed pneumoperitoneum in 12 patients (70.59%). The colon was the most frequent site of perforation at 29.41%. ECUN was the main etiology with 35.29%. Ostomy was the main surgical procedure in 58.82% of cases, followed by suture excision in 29.41%. Postoperative follow-up was simple in 3 patients (17.65%) and complicated in 14 (82.35%). Sepsis was the main cause of death (47.59%). The mortality rate was 76.47%. Conclusion: Mortality from neonatal peritonitis remains high in developing countries. Early diagnosis, early surgical management, and the presence of well-equipped neonatal intensive care units with qualified staff are essential for an improvement in the prognosis of neonatal peritonitis.

关键词： Etiology Newborns Gastrointestinal Perforation

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Management of Acute Idiopthic Intestinal Invaginal in Infants at Conakry University Hospital

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Open Journal of Pediatrics 2024年第5期14卷 937-950页

作者： Thierno Saidou Barry Mohamed Lamine Sadou Sacko Balla keita Mory Sangare Fanta Kourouma Moussa Conde Baldé Aissatou Bailo Seydou keita Daniel Agbo-Panzo Pediatric Surgery Department of the Conakry University Hospital Conakry Guinea Gamal Abdel Nasser University of Conakry Conakry Guinea Pediatrics Department Albert Royer Childrens Hospital Cheikh Anta Diop University of Dakar (UCAD) Dakar Senegal General Surgery Department of the Ignace Deen University Hospital Conakry Guinea

Introduction: Acute intestinal intussusception (AII) has been known for a long time but its diagnosis sometimes remains difficult and its etiologies, particularly the idiopathic forms, poorly elucidated. The objective of this study was to describe the clinical and therapeutic aspects of acute intestinal intussusception in order to contribute to the improvement of the management of this condition in the pediatric surgery department of the Donka National Hospital. Materials and Methods: This is a retrospective descriptive study of patients taken in charge of acute idiopathic intestinal intussusception over a period of 7 years (January 2017 to December 31, 2023). Results: We collected 72 patients operated on for IIA, i.e. a hospital frequency of 1.1% and an incidence of 10.3 cases per year. The mean age was 16.58 months with extremes of 3 months and 28 months. The sex ratio was 12.5 in favor of the boy. The triad of ombredanne dominated the clinical picture and paroxysmal abdominal pain with period of calm represented the main reason for consultation (72 cases) i.e. 100%, followed by vomiting (62 cases) and rectal bleeding (50 cases). Ultrasound was performed in 56 patients visualizing the intussusception sausage in 54 patients or 75%. The most common anatomoclinical variety of intussusception was the ileo-caeco-colic form with 35 cases (or 38%) followed by the ileocolic form 24 cases (33%). The surgical procedure performed was a manual reduction in 54 patients or 75% and an anastomosis resection in 18 patients;the appendectomy of principle was done in 62.25%. The postoperative course was simple in 64 patients or 88.88%. The mortality rate was 6.94 %. Conclusion: AII is a common abdominal surgical emergency in infants. Recognition of the Ombredanne triad especially abdominal pain with a period of calm in children and infants in particular is an important element for vital prognosis. Early diagnosis and close interdisciplinary cooperation between pediatric, radiology an

关键词： Acute Idiopathic Intussusception Infant Intussusception Sausage Pediatric Surgery

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Infectious ENT Emergencies: A One-Year Experience at the ENT Department of Mamou Regional Hospital

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International Journal of Otolaryngology and Head & Neck Surgery 2024年第5期13卷 359-372页

作者： Mamadou Mouctar Ramata Diallo Ibrahima Diallo Oumou Amadou Diallo Alsény Cisse Mamadou Bailo Sow Raphan Madi Kaba keita Abdoulaye keita Alpha Oumar Diallo ENT Department Mamou Regional Hospital Mamou Guinea Faculty of Health Sciences and Techniques Gamal Abdel Nasser University of Conakry Conakry Guinea ENT Department Donka National Hospital Conakry Guinea ENT Department Ignace Deen National Hospital Conakry Guinea ENT Department Lab Regional Hospital Lab Guinea ENT Department NZrkor Regional Hospital NZrkor Guinea

Introduction: ENT infectious emergencies encompass all infectious pathologies affecting the ear, nose, sinuses, throat, and neck. A good understanding of these emergencies is essential for organizing appropriate care. In Guinea, few studies have described ENT infectious pathologies in rural areas. Objective: To study ENT infectious emergencies in the Otorhinolaryngology department of the Mamou Regional Hospital. Materials and Methods: This is a retrospective and prospective descriptive study, conducted from August 1, 2021, to July 31, 2022. All patients admitted to the ENT department of the Mamou Regional Hospital for an infectious emergency were included. Results: Among the 1854 recorded consultations, 261 cases involved ENT infectious emergencies, representing a prevalence of 14.07%. The most represented age group was 0 to 10 years (31.03%), with a majority of female patients (59%) and a predominantly urban origin (81.61%). Students accounted for 32.18% of the cases. The main reasons for consultation were odynophagia/dysphagia (32.42%) and otalgia (22.94%). The primary etiologies were tonsillitis (28.73%) and otitis (27.20%). Treatments administered included probabilistic antibiotic therapy (38.80%), analgesics (32.03%), and corticosteroid therapy (10.47%). The outcome was favorable in 98.85% of cases, with only 0.38% lost to follow-up. Conclusion: The results of our study confirm the frequency of ENT infectious pathologies. Their management requires joint actions for population awareness, staff training, and services equipment, particularly in rural areas.

关键词： ENT Infection Emergency Mamou Regional Hospital

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Contribution of Imagery in the Diagnosis of Multisystemic Sarcoidosis in the Service Radiology Department of the Mother and Child Hospital “Le Luxembourg” in Bamako: A Case Report

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Open Journal of Medical Imaging 2024年第3期14卷 79-85页

作者： Mariko Mahamane Camara Mamoudou keita Aboubacar Sidiki N’Diaye Mahamadou Camara Mody Abdoulaye Fofana Youssouf Traoré Mohamed Maba Sidibé Siaka Sanogo Souleymane keita Adama Diaman Department of Radiology Mother and Child Hospital Luxembourg Bamako Mali Medical Imaging Exploration and Diagnostic Center Conakry Guinea Assistant at the Faculty of Health Sciences and Techniques at the Gamal Abdel Nasser University of Conakry Department of Radiology Military Hospital of Bamako Bamako Mali Department of Radiology Mali Hospital Bamako Mali Department of Radiology Point G Hospital Bamako Mali

The aim of this study was to report a case of multi-visceral sarcoidosis at the Mother-Child Hospital Center (CHME) “Le Luxembourg” in Bamako, Mali. Observation: This is a patient aged 62 at the time of consultation, a housewife, residing in the Banconi district, who was referred to us for thoracic-abdominopelvic imaging for chronic liver disease. After several diagnostic errors, the thoracic-abdominopelvic CT scan and liver MRI performed in our center showed, at the thoracoabdominal level, bilateral diffuse pulmonary micronodules and bilateral mediastinal-hilar lymphadenopathy;on the abdominal level, a dysmorphic liver with plaques of steatosis and a granular appearance of the liver parenchyma without periportal fibrosis. These imaging data combined with those from the liver nodule biopsy and biology confirmed the diagnosis of sarcoidosis type II. Treatment with corticosteroids gave satisfactory results and the patient recovered after 18 months. Clinical and CT monitoring 2 years from the start of the disease and 2 months from the end of treatment showed complete resolution of the lesions. Conclusion: The multi-visceral location of sarcoidosis is an entity whose diagnosis remains difficult;diagnostic and interventional imaging has an important place in its management.

关键词： Sarcoidosis Multi Visceral Imaging CHME Luxembourg

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