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A CIN-like TCP transcription factor(LsTCP4)having retrotransposon insertion associates with a shift from Salinas type to Empire type in crisphead lettuce(Lactuca sativa L.)

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Horticulture Research 2020年第1期7卷 2597-2610页

作者： Kousuke Seki Kenji Komatsu keisuke tanaka Masahiro Hiraga Hiromi Kajiya-Kanegae Hideo Matsumura Yuichi Uno Nagano Vegetable and Ornamental Crops Experiment Station Tokoo 1066-1SougaShiojiriNagano 399-6461Japan Department of Bioresource Development Tokyo University of AgricultureFunako1737AtsugiKanagawa 243-0034Japan NODAI Genome Research Center Tokyo University of AgricultureSakuragaoka 1-1-1SetagayaTokyo 156-8502Japan Nagano Fruit Tree Experiment Station Ogawara 492SuzakaNagano 382-0072Japan Research Center for Agricultural Information Technology NAROKannondai 3-1-1TsukubaIbaraki 305-8517Japan Gene Research Center Shinshu Seki et al.Horticulture Research(2020)7:15 Page 13 of 14 UniversityTsuneta 3-15-1UedaNagano 386-8567Japan Plant Science Division Department of Bioresource ScienceGraduate School of Agricultural ScienceKobe UniversityRokkodai 1-1NadaKobeHyogo 657-8501Japan

To improve several agronomic traits in crisphead lettuce(Lactuca sativa L.)under high-temperature growth conditions,we investigated the correlation among those traits in multiple cultivars and performed genetic mapping of their causal *** a field cultivation test of Empire type(serrated leaf)and Salinas type(wavy leaf)cultivars,Empire type cultivars showed increased tipburn susceptibility and late bolting compared with Salinas type *** attempted genetic mapping of leaf shape and bolting time by ddRAD-seq using the F2 population derived from a cross between‘VI185’(Empire type)and‘ShinanoGreen’(Salinas type).These analyses suggested that both traits are controlled by a single locus in *** of 51 commercial lettuce cultivars with a tightly linked marker(LG5_v8_252.743Mbp)at this locus showed an association between its genotype and the serrated leaf *** further fine mapping and transcriptome analysis,a gene encoding putative CIN-like TCP transcription factor was determined to be a candidate gene at this locus and was designated as *** insertion of retrotransposable element was found in the allele of‘VI185’,and its transcript level in the leaves was lower than that in‘ShinanoGreen’.Because shapes of leaf epidermal cells in‘VI185’were similar to those in the TCP family mutant of Arabidopsis thaliana,the leaf shape phenotype was likely caused by reduced expression of ***,because it is known that the TCP family protein also controls flowering time via interaction with FT in ***,it was highly possible that LsTCP4 gave pleiotropic effects on both leaf shape and bolting time in lettuce.

关键词： cultivar insertion Lactuca

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Chlorine Atom Transfer of Unactivated Alkyl Chlorides Enabled by Zirconocene and Photoredox Catalysis

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Precision Chemistry 2023年第2期1卷 112-118页

作者： Toshimasa Okita Kazuhiro Aida keisuke tanaka Eisuke Ota Junichiro Yamaguchi Department of Applied Chemistry Waseda UniversityShinjukuTokyo 162-0041Japan

Alkyl chlorides are robust precursors to carbon radicals;however,their relative inertness has hampered their practical *** modern photochemical strategies have greatly enhanced the utility of alkyl chlorides as radical precursors,these methods often depend on strongly reducing conditions leading to unproductive side ***,we report a catalytic radical generation from 1°,2°,and 3°unactivated alkyl chlorides with zirconocene and photoredox catalysis,which enables both hydrogenation and borylation on a range of structurally complex *** mild zirconocene-catalyzed protocol shows that zirconium can render the C−Cl bond cleavage more exergonic and can lower the activation energy of the transition state,amplifying the ability of metallocenes toward halogen atom transfer.

关键词： Zirconocene Alkyl chloride Halogen atom transfer Borylation Photoredox catalysis

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An Ancestral Gibberellin in a Moss Physcomitreila patens

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Molecular Plant 2018年第8期11卷 1097-1100页

作者： Sho Miyazaki Mariho Hara Shinsaku Ito keisuke tanaka Tadao Asami Ken-ichiro Hayashi Hiroshi Kawaide Masatoshi Nakajima Department of Applied Biological Chemistry The University of Tokyo Tokyo 113-8657 Japan Department of Bioscience Faculty of Applied Bioscience Tokyo University of Agriculture Tokyo 156-8502 Japan NODAI Genome Research Center Tokyo University of Agriculture Tokyo 156-8502 Japan Department of Biochemistry Okayama University of Science Okayama 700-0005 Japan Institute of Agriculture Tokyo University of Agriculture and Technology Tokyo 183-8509 Japan

Dear Editor,Most of the plant hormones found in flowering plants contribute to growth regulation in the moss Physcomitrella patens. This moss does not produce gibberellin （GA）（Figure 1A）. An ent-kaurene synthase （PpCPS/KS） and an ent-kaurene oxidase （PpKO） have been identified in P. patens, but in contrast to flowering plants, P. patens has no ent-kaurenoic acid oxidase （KAO） homologs （Hayashi et al., 2010; Miyazaki et al., 2011, Fiqure 1A）.

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Combined administration of captopril with an antibypertensive Val-Tyr di-peptide to spontaneously hypertensive rats attenuates the blood pressure lowering effect

Combined administration of captopril with an antibypertensiv...

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中国食品科学技术学会第五届年会暨第四届东西方食品业高层论坛

作者： Toshiro Matsui keisuke Watanabe keisuke tanaka Yoko Kusano Kiyoshi Matsumoto Division of Bioscience and Bioenvironmental Sciences Faculty of Agriculture Graduate School of Kyushu UniversityFukuoka 812-8581Japan Division of Bioscience and Bioenvironmental Sciences Faculty of Agriculture.Graduate School of Kyushu University Fukuoka 812-8581Japan

Some di-peptides have been proven to exert an antihypertensive effect in mild-hypertensive *** aim of this study was to clarify whether combined administration of an ACE inhibitor,captopril,with an antihypertensive di-peptide Val-Tyr(VY)would alter their potent antihypertensive effects in spontaneously hypertensive rats(SHRs).Single oral administration of captopril(2.5 mg/kg),VY(25 mg/kg),or captopril(2.5 mg/kg)+VY(25 mg/kg) to 18-week-old male SHRs was *** blood pressure(SBP)was measured up to 9 h, and plasma captopril concentrations were determined.A transport study of captopril and/or VY across living rat jejunum frorn SHRs was also performed to evaluate the kinetics of absorption. Combined administration of captopril with VY failed to lower the BP during the 9-h experiment.A transport study of captopril or VY revealed that VY inhibited captopril transport,and vice versa,in a competitive manner and exhibited an approximately,1/3-fold lower Ki value for captopril compared with that for VY;indicating that both compounds compete for the same membrane transport pathway.A 50% decrease in plasma captopril levels by combined administration with VY supported that the attenuation of the BP lowering effect was due to inhibition of captopril uptake by ***,our findings suggest that subjects treated with ACE inhibitors for hypertension should avoid combined-intake with antihypertensive foods that are rich in small peptides due to the competitive inhibition of drug uptake by these peptides.

关键词： Hypertension Absorption Captopril Peptide Angiotensin I-converting enzyme inhibitor Combined administration

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A single atom photocatalyst co-doped with potassium and gallium for enhancing photocatalytic hydrogen peroxide synthesis

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Nano Research 2024年第8期17卷 7027-7038页

作者： Wenan Cai Yuichiro tanaka Xing Zhu Teruhisa Ohno Department of Applied Chemistry Faculty of EngineeringKyushu Institute of TechnologyKitakyushu 804-8550Japan College of Bioresources Chemical and Materials Engineering Shaanxi University of Science&TechnologyXi’an 710021China

Polymer carbon nitride(PCN)is widely used in ***,pristine PCN has disadvantages such as insufficient visible light absorption and low photogenerated carrier separation efficiency that greatly limited the photocatalytic *** a non-toxic metal,gallium has the potential to solve the defects of *** ions coordinated with nitrogen in carbon nitride to form Ga-N active sites and improved the photocatalytic *** doped potassium ions form a transmission channel for charge redistribution and transfer between adjacent layers,which is beneficial for better separation of photoexcited *** this study,a series of PCN co-doped with gallium and potassium(Ga-K-PCN)were *** experimental results indicated photocatalytic generation of hydrogen peroxide proceeds through the 2e−oxygen reduction reaction ***,Nyquist plots and photocurrent results further proved that the presence of Ga-N sites and potassium ion doping could significantly improve the separation/transfer of intra-planar and interlayer charge carriers and thus enhance photocatalytic *** Ga10-K3-PCN photocatalysts promoted yield of H_(2)O_(2),with reactivity at 28.2μmol/(g·h)and solar-to-chemical conversion efficiency at 0.64%,surpassed that of a typical photo-catalyst based on PCN(0.18%).

关键词： photocatalysis gallium oxygen reduction reaction H_(2)O_(2) production

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不锈钢在循环扭转载荷下近门槛值的疲劳裂纹扩展行为

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金属学报 2005年第7期41卷 721-726页

作者：于慧臣张岩基孙燕国谢世殊田中启介北京航空材料研究院北京100095 本溪钢铁集团有限责任公司本溪117100 沈阳重型机械集团有限责任公司宝钢技术中心上海201900 名古屋大学工学部

研究了带环状预裂纹不锈钢圆棒试样在循环扭转载荷下、门槛值附近的疲劳裂纹扩展行为,用应力强度因子表征了裂纹扩展开始的门槛值.随着裂纹的扩展,裂纹扩展速率由于裂纹面的滑移接触而减小.通过外插裂纹扩展速率与裂纹长度之间的关系,... 详细信息

研究了带环状预裂纹不锈钢圆棒试样在循环扭转载荷下、门槛值附近的疲劳裂纹扩展行为,用应力强度因子表征了裂纹扩展开始的门槛值.随着裂纹的扩展,裂纹扩展速率由于裂纹面的滑移接触而减小.通过外插裂纹扩展速率与裂纹长度之间的关系,可近似得到裂纹长度为零时无裂纹面滑移接触影响的裂纹扩展速率.施加的应力强度因子范围可分解为推动裂纹扩展的有效值和由于裂纹面的滑移接触而屏蔽掉的两部分.预测了疲劳裂纹的萌生和断裂极限,预测值和实验值相当一致.

关键词：疲劳裂纹扩展应力强度因子门槛值扭转

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不锈钢在扭转／拉伸复合载荷下近门槛值的疲劳裂纹扩展行为

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金属学报 2006年第2期42卷 186-190页

作者：于慧臣孙燕国张岩基谢世殊田中启介北京航空材料研究院北京100095 沈阳重型机械集团有限责任公司沈阳110025 本溪钢铁集团有限责任公司本溪117100 宝山钢铁集团公司技术中心上海201900 名古屋大学工学部

研究了带环状预裂纹的奥氏体不锈钢圆棒试样在循环扭转／拉伸复合载荷下,门槛值附近的疲劳裂纹扩展行为,分析了复合加载时不同载荷比对裂纹扩展的影响,对裂纹扩展的初始阶段进行了详细研究,用应力强度因子表征了裂纹扩展开始的门槛值,... 详细信息

研究了带环状预裂纹的奥氏体不锈钢圆棒试样在循环扭转／拉伸复合载荷下,门槛值附近的疲劳裂纹扩展行为,分析了复合加载时不同载荷比对裂纹扩展的影响,对裂纹扩展的初始阶段进行了详细研究,用应力强度因子表征了裂纹扩展开始的门槛值,提出了椭圆方程表达的疲劳裂纹扩展开始的门槛值模型,该方程可在裂纹尖端位移准则的基础上导出,疲劳裂纹扩展停止的门槛值条件也可用椭圆方程表达。

关键词：扭转/拉伸复合加载疲劳裂纹扩展滑移接触应力强度因子门槛值

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Fabrication of Mie-resonant silicon nanoparticles using laser annealing for surface-enhanced fluorescence spectroscopy

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Microsystems & Nanoengineering 2024年第2期10卷 133-141页

作者： Tatsuya Fukuta Ryo Kato Takuo tanaka Taka-aki Yano Institute of Post-LED Photonics Tokushima University2-1 Minami-JosanjimaTokushima770-8506Japan Innovative Photon Manipulation Research Team RIKEN Center for Advanced PhotonicsWakoSaitama351-0198Japan Metamaterials Laboratory RIKEN Cluster for Pioneering ResearchWakoSaitama351-0109Japan

Silicon nanostructures with unique Mie resonances have garnered considerable attention in the field of ***,we present a simple and efficient method for the fabrication of silicon(Si)nanoparticle substrates using continuous-wave(CW)laser *** resulting silicon nanoparticles exhibit Mie resonances in the visible region,and their resonant wavelengths can be precisely ***,laser-annealed silicon nanoparticle substrates show a 60-fold enhancement in *** tunable and fluorescence-enhancing silicon nanoparticle platform has tremendous potential for highly sensitive fluorescence sensing and biomedical imaging applications.

关键词： nanoparticles annealing resonant

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Metabolomics in liver diseases: A novel alternative for liver biopsy?

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World Journal of Hepatology 2024年第1期16卷 12-16页

作者： Yasuo tanaka Department of Gastroenterology National Center for Global Health and MedicineTokyo 162-8655Japan

Hepatitis C virus(HCV)remains a significant public health problem as it can cause acute and chronic *** HCV infection is a major cause of liver fibrosis,and evaluation of liver fibrosis is essential because the prognosis of patients with chronic HCV infection is closely related to the stage of *** fibrosis is traditionally evaluated based on pathological analysis of biopsy specimens,which is considered the gold ***,liver biopsy is invasive and susceptible to sampling error and inter-and intraobserver variation in pathological interpretation;it is also ***,noninvasive diagnostic investigations have been developed,including the use of fibrotic markers,scoring systems based on routine blood tests,and transient elastography with magnetic resonance imaging or ***,metabolomics,an emerging technology,has been used to detect the fibrosis *** this editorial,I comment on the article titled“Metabolomics in chronic hepatitis C:Decoding fibrosis grading and underlying pathways”by Ferrasi et al published in the recent issue of the World Journal of Hepatology.I discuss previous studies on the use of metabolome analysis for the diagnosis of HCV-related liver fibrosis and the potential development of biopsy-free diagnostic techniques.

关键词： Metabolomics Hepatitis C virus Liver fibrosis Liver cirrhosis Serum biomarker

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Exploiting fly models to investigate rare human neurological disorders

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Neural Regeneration Research 2025年第1期20卷 21-28页

作者： Tomomi tanaka Hyung-Lok Chung Department of Neurology Houston Methodist Research InstituteHoustonTXUSA Department of Neurology Weill Cornell Medical CollegeNew YorkNYUSA

Rare neurological diseases,while individually are rare,collectively impact millions globally,leading to diverse and often severe neurological *** attributed to genetic mutations that disrupt protein function or structure,understanding their genetic basis is crucial for accurate diagnosis and targeted *** investigate the underlying pathogenesis of these conditions,researchers often use non-mammalian model organisms,such as Drosophila(fruit flies),which is valued for their genetic manipulability,cost-efficiency,and preservation of genes and biological functions across evolutionary *** tools available in Drosophila,including CRISPR-Cas9,offer a means to manipulate gene expression,allowing for a deep exploration of the genetic underpinnings of rare neurological *** boasts a versatile genetic toolkit,rapid generation turnover,and ease of large-scale experimentation,making it an invaluable resource for identifying potential drug *** can expose flies carrying disease-associated mutations to various compounds,rapidly pinpointing promising therapeutic agents for further investigation in mammalian models and,ultimately,clinical *** this comprehensive review,we explore rare neurological diseases where fly research has significantly contributed to our understanding of their genetic basis,pathophysiology,and potential therapeutic *** discuss rare diseases associated with both neuron-expressed and glial-expressed *** cases include mutations in CDK19 resulting in epilepsy and developmental delay,mutations in TIAM1 leading to a neurodevelopmental disorder with seizures and language delay,and mutations in IRF2BPL causing seizures,a neurodevelopmental disorder with regression,loss of speech,and abnormal *** we explore mutations in EMC1 related to cerebellar atrophy,visual impairment,psychomotor retardation,and gain-of-function mutations in ACOX1 causing Mitchell ***-of-fun

关键词： ACOX1 Drosophila melanogaster glia lipid metabolism model organisms neuroinflammation neurologic disorders neuron rare disease VLCFA

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