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Direct Correlation among Telomere Length, Cellular Aging, and Rejuvenation Effects of Honey Child Powder

Journal of Biosciences and Medicines 2024年第7期12卷 55-70页

作者： Naofumi Shiomi keiko watanabe Yuki Fujiwara Takae Yamasaki Hideto Matsuyama Research Center for Membrane and Film Technology Kobe University Kobe Japan School of Human Sciences Kobe College Nishinomiya Japan Department of Chemical Science and Engineering Kobe University Kobe Japan

Purpose: Telomere length (TL) is an indicator of age;however, hormonal influences complicate individual aging. It remains unclear whether TL shortening is a direct factor in both individual and cellular aging. Therefore, we examined the direct relationship between TL and cellular senescence at the cellular level. Methods: Telomerase activity, TL, and gene expression were measured in cultured human lung-, fetal-, and skin-derived fibroblasts, human skin keratinocytes, and telomerase reverse transcriptase (TERT) gene-immortalized cells using detection kits, Cawthon’s method, and reverse transcription-quantitative polymerase chain reaction, respectively. Novel substances that elongate telomeres were screened to confirm cell rejuvenation effects. Results: Long-term cell culture of TIG-1-20 normal human fibroblasts resulted in TL shortening, decreased division rate, and senescence progression, whereas in OUMS-36T-2 cells, TL elongation via TERT gene transfer increased the division rate, reduced endoplasmic reticulum stress, and upregulated genes associated with young individuals, indicating that cellular rejuvenation occurs via TL elongation. In addition, a honey child powder (HCP) extract was found through screening, and the HCP extract strongly suppressed the menin gene, resulting in increased telomerase activity and extended cell lifespan. Upon addition of the HCP extract to skin fibroblasts, gene expression of moisturizing components, including collagen, hyaluronic acid, and elastin, increased, and exhibited a rejuvenating effect with an increase in elastin amount. Conclusions: TL elongation or shortening is involved in cell proliferation rate and cellular aging, and TL elongation rejuvenates cells. In addition, HCP extract has a rejuvenating effect on cells and is expected to be a rejuvenating compound.

关键词： Telomere Cellular Aging Telomerase Reverse Transcriptase Gene Rejuvenation Honey Child Powder

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Performance Evaluation of Electromagnetic Shield Constructed from Open-Cell Metal Foam Based on Sphere Functions

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Computer Modeling in Engineering & Sciences 2022年第7期132卷 43-53页

作者： Yuya Hanaoka Taku Itoh Kohei Tateyama Susumu Nakata keiko watanabe Graduate School of Industrial Technology Nihon UniversityNarashinoChiba 275-8575Japan College of Industrial Technology Nihon UniversityNarashinoChiba 275-8575Japan College of Science and Engineering Ritsumeican UniversityKusatsuShiga 525-8577Japan College of Information Science and Engineering Ritsumeican UniversityKusatsuShiga 525-8577Japan

This study evaluates the performance of a model of open-cell metal foams generated by sphere *** this end,an electromagnetic shield constructed from the model was inserted between two horn antennas in an electromagnetic wave propagation *** foam-hole diameter in the electromagnetic shield model was varied as d=2.5 and 5.0 mm,and the frequency of the electromagnetic waves was varied from 3 to 13 *** the numerical experiments of shield effectiveness,the shields with foam holes of both diameters attenuated the electromagnetic waves across the studied frequency *** shield effectiveness was enhanced at low frequencies and in the shield with smaller hole diameter.

关键词： Electromagnetic propagation electromagnetic shielding materials metal foams numerical simulation

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An autopsy case of granulocyte-colony-stimulating-factor- producing extrahepatic bile duct carcinoma

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World Journal of Gastroenterology 2008年第18期14卷 2924-2927页

作者： Satoru Matsuyama Tomonori Shimonishi Hirofumi Yoshimura Kensaku Higaki Kenji Nasu Mariko Toyooka Shigehisa Aoki keiko watanabe Hajime Sugihara Department of Surgery Takagi Hospital141-11Sakemi Ohkawa-CityFukuoka 831-0016Japan Department of Pathology Takagi Hospital141-11SakemiOhkawa-City Fukuoka 831-0016 Japan Department of Pathology and Biodefence Faculty of Medicine Saga University 5-1-1 Nabeshima Saga-City Saga 849-8501 Japan

A 79-year-old man was referred to this department due to the presence of extrahepatic bile duct carcinoma with a tumor at the left chest wall. The lesion was suspected to be a metastasis of bile duct carcinoma to the left wall, however, computed tomography (CT) revealed no regional lymph node or liver metastases. In addition, cytological and pathological examinations did not show malignancy. At the time of admission, the white blood cell count was 21 460 cells/μL (neutrophils, 18 240 cells/μL) and this elevated to 106 040 before death. In addition, serum granulocyte colony-stimulating factor (G-CSF) was elevated. At 28 d after admission, the patient died. An autopsy showed a poorly differentiated adenocarcinoma with sarcomatous change, which had slightly invaded into the pancreas around the bile duct, and was found in the distal bile duct with multiple metastases to the chest wall, lung, kidney, adrenal body, liver, mesentery, vertebra and mediastinal and para-aortic lymph nodes, without locoregional lymph node and liver metastasis. The cancer cells showed positive immunohistochemical staining for anti-G-CSF antibody. This is believed to be the first report of an extrahepatic bile duct carcinoma that produces G-CSF. Since G-CSF-producing carcinoma and sarcomatous change of the biliary tract leads to poor prognosis, early diagnosis and treatment are needed. When infection is ruled out, the G-CSF in serum should be examined. In addition, examinations such as bonescintigraphy and chest CT should also be considered for distant metastasis.

关键词： Bile duct carcinoma Granulocyte colony-stimulating factor Multiple metastases Autopsy

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Effects of oleic acid on murine macrophage dysfunction

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Journal of Biomedical Science and Engineering 2013年第6期6卷 654-660页

作者： Naofumi Shiomi keiko watanabe School of Human Sciences Kobe College Nishinomiya Japan

Obese individuals exhibit much higher risks not only for metabolic syndrome, but also for cancer and allergies, than normal-weight subjects. This fact suggests that signals secreted from adipocytes change the characteristics of lymphocytes, such as macrophages and T-cells. We focused on a free fatty acid, oleic acid, as a signal inducing such changes and examined its effects on murine J774.2 macrophages. When the cells were cultured in medium containing high concentrations (1, 2 and 4 mM) of oleic acid, apoptosis occurred, and the apoptotic cells were gathered into clusters of very large size by the work of enzymes for phagocytosis. When the cells were cultured in medium containing 0.5 mM of oleic acid, the fatty acid did not affect cell growth;however, it inhibited nitrogen monoxide (NO) secretion and the gene expressions of interleukins and TNF-α. NO disturbs the invasion of macrophages into blood vessels, and interleukins promote the differentiation and proliferation of T- and B-cells. Therefore, these results suggest that the high risks for cancer and allergies observed in obese subjects are associated with the dysfunction of macrophages induced by fatty acids. Moreover, we also examined the protective effects of carnitine against dysfunction. However, carnitine did not exhibit sufficient effects.

关键词： Macrophage Oleic Acid Cancer Allergy Dysfunction

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Effects of Hydrocortisone, Glycerophosphate and Retinol on the Differentiation of Mesenchymal Stem Cells and Vascular Endothelial Cells to Osteoblasts

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Journal of Biomedical Science and Engineering 2014年第13期7卷 1056-1066页

作者： Naofumi Shiomi keiko watanabe School of Human Sciences Kobe College Nishinomiya Japan

Vascular calcification, which causes occlusion and rupture of the vascular, is often observed in patients in the advanced stages of arteriosclerosis. One of the best procedures for inhibiting the accumulation of vascular calcification is to obstruct the differentiation of mesenchymal stem cells (MSCs) and/or vascular endothelial cells (VECs) in the vascular to osteoblasts. In this study, we evaluated the biochemical and genetic characteristics of the process of differentiation of MSCs and VECs to osteoblasts. C3H10T1/2 MSCs, TKD2 VECs and MC3T3-E1 preosteoblasts (POBs) were cultured in medium containing both hydrocortisone and glycerophosphate. These compounds showed strong effects promoting the differentiation of VECs as well as POBs, although the effect was weak in the MSCs. Moreover, C3H10T1/2 MSCs and TKD2 VECs were cultured in medium containing 10 mM retinol, after which the alkali phosphatase (ALP) activity of the MSCs and production of calcified nodules of TKD2 were significantly increased, whereas the marker genes for the osteoblasts were not. These results suggest that retinol does not have an effect in inducing the differentiation of VECs to osteoblasts, but rather exhibits a strong promoting effect on differentiation.

关键词： Vascular Calcification Osteoblasts Vascular Endothelial Cells Mesenchymal Stem Cells Retinol Hydrocortisone

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Characteristics of Beige Adipocytes Induced from White Adipocytes by Kikyo Extract

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Journal of Biomedical Science and Engineering 2016年第7期9卷 342-353页

作者： Naofumi Shiomi Marie Ito keiko watanabe School of Human Sciences Kobe College Nishinomiya Japan

Beige adipocytes are believed to have a high ability to consume fat. As such, compounds capable of inducing the development of beige adipocytes may be useful as drugs for anti-obesity and anti-type 2 diabetes. However, the true nature of beige adipocytes remains unclear. The purpose of this study is to confirm whether or not white adipocyte can differentiate to beige adipocytes and to clarify the characteristics of beige adipocytes. We first searched for an inducer of beige adipocytes and found that kikyo extract, a component of bofu-tsusho-san, was a strong inducer. We then attempted to prove that beige adipocytes could be induced from white adipocytes. Second, we clarified the characteristics of beige adipocytes induced from white adipocytes. The results suggested that beige adipocytes were high-performance adipocytes with a greater ability to synthesize and consume triglyceride and take up glucose than white adipocytes.

关键词： Beige Adipocyte UCP1 GLUT4 Diabetes Obesity Insulin Resistance

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DEVELOPMENT OF LIFE-CONTROLLABLE-SMART（ LCS） CONCRETE USING RECYCLED PET RESIN BLOCKS

DEVELOPMENT OF LIFE-CONTROLLABLE-SMART（ LCS） CONCRETE USIN...

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作者： Takayuki Kusaka Hironori Namiki Kei Urabe Hiromasa Takeno Yukio Tada Masaki Hojo keiko watanabe Kensuke Okubo Department of Mechanical Engineering Ritsumeikan UniversityJapan Kyobashi Corporation Japan National Institute of Advanced Industrial Science and Technology Japan Department of Electrical and Electronics Engineering Kobe UniversityJapan keikon Corporation Japan Department of Mechanical Engineering Kyoto UniversityJapan Department of Mechanical Engineering Ritsumeikan UniversityJapan Department of Mechanical Engineering Ritsumeikan UniversityJapan

＜正＞A functional concrete, named "Life-Controllable-Smart" concrete, has been developed on the basis of the composite technology. The material, which was a kind of composite materials consisting of concrete and recycled PET blocks, could be easily and safely broken on demand by irradiating 2. 45 GHz microwave. The breakability, heatability, flowability and strength of the LCS concrete were investigated to improve the total performance of the material. Numerical and experimental results showed that the LCS concrete could be broken by heating up to 50-90 K above the room temperature, when the volume fraction of PET blocks was controlled within a range of 2% -5 % , where the compressive strength was reduced by 10% -20 %. The casting flowability of the LCS concrete could be adequately controlled by mixing a cellulose ether type thickener. The microwave heatability of the PET blocks could be effectively improved by mixing carbon milled fibers. Moreover, the applicability of the LCS concrete to temporary constructions was studied.

关键词： Smart Materials Microwave Heating Composite Concrete, PET

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有机氚化物对离体培养小鼠胎儿中脑细胞增殖及分化的影响

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中华放射医学与防护杂志 1997年第5期17卷 319-323页

作者：王冰渡边惠子东京大学理学部生物科学专攻放射线生物学研究室东邦大学医学部生物研究室东邦大学医学部放射线同位素中央研究室

作者就四种有机氚化物对体外培养细胞的增殖及分化的影响进行了研究。方法细胞取自１１日胎龄小鼠中脑组织，对其进行高密度细胞培养，并加入有机氚化物与培养液。结果中脑细胞的分化比增殖对辐射更敏感。辐射引起剂量依赖性的培养细胞... 详细信息

作者就四种有机氚化物对体外培养细胞的增殖及分化的影响进行了研究。方法细胞取自１１日胎龄小鼠中脑组织，对其进行高密度细胞培养，并加入有机氚化物与培养液。结果中脑细胞的分化比增殖对辐射更敏感。辐射引起剂量依赖性的培养细胞ＤＮＡ和蛋白质含量的减少。以各指标５０％的抑制剂量，用Ｘ射线为参考辐射估算的氚ＲＢＥ值在４．６～８．７范围。混合辐射，特别是当使用３Ｈ－ＴｄＲ时，能更有效地抑制中脑细胞的分化。０．０５Ｇｙ即可产生可检测到的抑制效应。结论高密度中脑细胞培养系统对辐射极为敏感，来自有机氚化物的β射线对该系统中细胞的增殖及分化的抑制作用与参考辐射Ｘ射线相比较，具有极高的相对生物效应比值。

关键词：有机氚化物胎儿中脑细胞相对生物效能

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CT-maximum intensity projection is a clinically useful modality for the detection of gastric varices

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World Journal of Gastroenterology 2005年第47期11卷 7515-7519页

作者： Toru Ishikawa Takashi Ushiki Ken-ichi Mizuno Tadayuki Togashi Kouji watanabe Kei-ichi Seki Hironobu Ohta Toshiaki Yoshida keiko Takeda Tomoteru Kamimura Department of Gastroenterology Saiseikai Niigata Second HospitalNiigataJapan Department of Radiology Saiseikai Niigata Second HospitalNiigataJapan

AIM： To evaluate the efficacy of CT-maximum intensity projection （CT-MIP） in the detection of gastric varices and their infiowing and outflowing vessels in patients with gastric varices scheduled to undergo balloonoccluded retrograde transvenous obliteration （B-RTO）. METHODS： Sixteen patients with endoscopically confirmed gastric varices were included in this study. All patients were evaluated with CT-MIP using threedimensional reconstructions, before and after B-RTO. RESULTS： CT-MIP clearly depicted gastric varices in 16 patients （100%）, the left gastric vein in 6 （32.5%）, the posterior gastric vein in 12 （75.0%）, the short gastric veins in 13 （81.3%）, gastrorenal shunts in 16 （100%）, the hemiazygos vein （HAZV） in 4 （25.0%）, the pericardiophrenic vein （PCPV） in 9 （56.3%）, and the left inferior phrenic vein in 9 patients （56.3%）. Although flow direction itself cannot be determined from CT-MIP, this modality provided clear images of the inflowing and the outflowing vessels. Moreover, in one patient, short gastric veins were not seen on conventional angiographic portography images of the spleen, but were clearly revealed on CT-MIP. CONCLUSION： We suggest that CT-MIP should be considered as a routine method for detecting and diagnosing collateral veins in patients with gastric varices scheduled for B-RTO. Furthermore, CT-MIP is more useful than endoscopy in verifying the early therapeutic effects of B-RTO.

关键词： Gastric varices CT-MIP, Portal hypertension

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Angiotensin-Ⅱ administration is useful for the detection of liver metastasis from pancreatic cancer during pharmacoangiographic computed tomography

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World Journal of Gastroenterology 2007年第22期13卷 3080-3083页

作者： Toru Ishikawa Takashi Ushiki Hiroteru Kamimura Tadayuki Togashi Atsunori Tsuchiya Kouji watanabe Kei-ichi Seki Hironobu Ohta Toshiaki Yoshida keiko Takeda Tomoteru Kamimura Department of Gastroenterology and Hepatology Saiseikai Niigata Second Hospital Niigata Japan Department of Radiology Saiseikai Niigata Second Hospital Niigata Japan

AIM: To improve the preoperative diagnosis of liver metastasis from pancreatic cancer, we estimated computed tomography during arterial angiography (CTA) with/without administration of angiotensin-Ⅱ (AT-Ⅱ). METHODS: Thirty-fi ve patients with pancreatic cancer were examined in this study. After conventional CTA was performed, pharmacoangiographic CTA was performed with a 1-3 microgram/5 mL solution of angiotensin Ⅱ injected through a catheter into the celiac artery during spiral computed tomography. We prospectively analyzed the relative region of interest (ROI) ratio of tumor to liver with/without AT-Ⅱ. RESULTS: In all patients, the relative ratio of each computed tomography (CT) number in the ROI was larger at pharmacoangiographic CT than at conventional angiographic CT. Administration of angiotensin-Ⅱ enhanced the metastatic liver tumor as compared with normal tissue. Intratumoral blood flow increased in all patients with malignant tumors due to the pressure effect of AT-Ⅱ. Furthermore, the metastatic lesions in the liver of three patients were represented by only pharmacoangiographic CT, not by conventional CT and conventional CT angiography. In even peripheral and central areas of metastatic liver tumor, the lesions were enhanced after administration of AT-Ⅱ. CONCLUSION: These results support that high detection rate of liver metastasis revealed by pharmacoangiographic CT suggests the improvement of diagnosis on preoperative staging. Moreover, chemotherapy under AT-Ⅱ induced hypertension may have a better effect on the treatment of metastatic liver tumors.

关键词： Angiotensin Ⅱ Phamacoangiographic CT Pancreatic cancer

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