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Modern Research in Inflammation 2014年第2期3卷 37-47页

作者： Y. Kamiya Y. ishihara H. Kamei Y. Ozawa H. Mizutani K. Kubo H. Maeda T. Noguchi Department of Pathology School of Dentistry Aichi-Gakuin University Nagoya Japan Department of Periodontology School of Dentistry Aichi-Gakuin University Nagoya Japan

Background and Objective: Interleukin-1 (IL-1) binds to 2 distinct and separate receptors, types I and II (IL-1RI and IL-1RII, respectively). The binding of IL-1 to IL-1RI induces cellular signaling and biological effects, whereas binding to IL-1RII does not induce cellular signaling and indirectly inhibits IL-1 biological activities such as that of the decoy receptor. Recently, Suzukiet *** that soluble IL-1RII (sIL-1RII) was detected in gingival crevicular fluid from a periodontitis patient. However, it remains unclear which cells produce sIL-1RII detected in periodontal tissues. We examined the localization of IL-1RII producing cells in gingival tissues as well as related production control mechanisms. Material and Methods: IL-1RII mRNA expression in gingival epithelial cells (GE1) was performed by real-time PCR analysis, while the amount of sIL-1RII production in supernatant from GE1 cells was examined by dot-blot analysis. Involvement of the phosphorylation of STAT6 in the signaling pathway was determined by western blot analysis. Statistical analysis was performed with Student’st-test. Results: Culturing with IL-4 and IL-13 significantly increased IL-1RII mRNA to levels 10.5-and 8.89-fold, respectively, above that of the control (p< 0.01), while addition of interferon-γ (IFN-γ) significantly suppressed IL-1RII mRNA by 0.22-fold as compared to the control (p< 0.05). Soluble IL-1RII in the supernatant of cultured GE1 cells was increased by IL-4 and IL-13, and decreased by IFN-γ, while western blotting determines the suppression of IL-1RII production by IFN-γ. Without the addition of IL-4 or IL-13 with or without IFN-γ, P-Tyr-STAT6 was not detected. Conclusion: IL-1RII mRNA expression and sIL-1RII production were increased by IL-4 and IL-13, and decreased by IFN-γ. Finally, IL-4 signaling was regulated by IFN-γ through phosphorylation of STAT6 and IL-13 signaling blockage by IFN-γ downstream of STAT6 translocation.

关键词： IL-1RII IL-4 IL-13 IFN-γ STAT-6

The Ability of Edible Mushrooms to Act as Biocatalysts: Preparation of Chiral Alcohols Using Basidiomycete Strains

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Advances in Microbiology 2012年第2期2卷 66-71页

作者： Kohji ishihara Yukiko Nishikawa Mari Kaneko Anna Kinoshita Nozomi Kumazawa Daichi Kobashigawa Kohei Kuroda Masashi Osawa Tatsunori Yamamoto Nobuyoshi Nakajima Hiroki Hamada Noriyoshi Masuoka Department of Life Science Okayama University of Science Okayama Japan Graduate School of Health and Welfare Science Okayama Prefectiral University Soja Japan Research Institute of Biotechnology Fujiwara Techno-Art Co. Ltd. Okayama Japan

To examine the potential ability of edible mushrooms to act as biocatalysts, 19 basidiomycete strains were screened. Modified media (PG, O, and PGO medium) for liquid cultivation of these basidiomycete strains were designed and tested. Wet cells (>10 g) of 4 basidiomycete strains (Pleurotus salmoneostramineus H7, P. salmoneostramineus H13, Ganoderma lucidum NBRC31863, Flammulina velutipes NBRC31862) were harvested from PGO medium for 7 days. The stereoselective reduction of α-keto esters using the 4 strains was tested. It was found that each of these strains had a reducing activity toward 6 aliphatic α-keto esters. In the presence of L-alanine as an additive, the reduction of ethyl 2-oxobutanoate and ethyl 2-oxopentanoete by P. salmoneostramineus H7 produced the corresponding alcohol with a high conversion ratio and with excellent enantiomeric excess (>99% e.e. (R)). Furthermore, ethyl pyruvate, ethyl 2-oxobutanoate, and ethyl 2-oxopentanoate were predominantly reduced to the corresponding (R)-hydroxy ester (>99% e.e.) by G. lucidum. Thus, we found that these edible mushrooms have great potential to be used as biocatalysts for the stereoselective reduction of carbonyl compounds.

关键词： Basidiomycete Edible Mushroom Biocatalyst Chiral Alcohol

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junB启动子X射线辐射敏感区域的检测

中华放射医学与防护杂志 2005年第3期25卷 238-240页

作者：万虹石原弘田中泉北京市神经外科研究所 100050 日本科学技术厅放射线医学综合研究所

目的探讨junB启动子X射线特异性辐射敏感区。方法构建含250、590、900和1560bpjunB启动子基因片段及CAT报道基因的4种质粒,转染辐射诱导的白血病细胞系L8704细胞,2GyX射线照射,RNA提取,Northern印迹及RNA定量分析。结果含900和1560bpjun... 详细信息

目的探讨junB启动子X射线特异性辐射敏感区。方法构建含250、590、900和1560bpjunB启动子基因片段及CAT报道基因的4种质粒,转染辐射诱导的白血病细胞系L8704细胞,2GyX射线照射,RNA提取,Northern印迹及RNA定量分析。结果含900和1560bpjunB启动子基因片段的质粒组CATRNA表达分别在照射后30和60min达高峰。结论junB启动子590～900bp基因片段为辐射敏感区。

关键词： junB 启动子 X射线辐射敏感区域 Northern印迹检测基因片段白血病细胞系辐射敏感 X射线照射 RNA提取 RNA表达报道基因辐射诱导定量分析 CAT 特异性 4细胞质粒

Formation of the giant Chalukou porphyry Mo deposit in northern Great Xing'an Range,NE China:Partial melting of the juvenile lower crust in intra-plate extensional environment

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Formation of the giant Chalukou porphyry Mo deposit in north...

Predictors of LGBT Recognition by Health Sciences University Students in Japan

中国科学院地质与地球物理研究所2014年度（第14届）学术年会

作者： Zhen-Zhen Li Ke-Zhang Qin Guang-Ming Li Shunso ishihara Lu-Ying Jin Guo-Xue Song Zhao-Jun Meng Key Laboratory of Mineral Resources Institute of Geology and GeophysicsChinese Academy of Sciences University of Chinese Academy of Sciences Geological Survey of Japan AIST 706 Geological Party of Heilongjiang Geoexploration Bureau for Nonferrous Metals

The Chalukou porphyry Mo deposit(2.46 Mt @ 0.087%Mo),located in the northern Great Xing’an Range,NE China,is the largest Mo deposit discovered in China so *** host rocks consist of aplite porphyry,granite porphyry and quartz porphyry,and are intruded into Lower Ordovician intermediate-felsic volcanic-sedimentary rocks and preore monzogranite and are cut by post-ore feldspar porphyry,diorite porphyry and quartz monzonite ***,we present the zircon U-Pb ages,whole-rock geochemistry,Sr-Nd isotopic and zircon Hf isotopic data for the pre-ore,syn-ore and post-ore intrusive *** Chalukou ore-forming porphyries intruded during 147-148 Ma and have high-silica,alkali-rich,metaluminous to slightly peraluminous compositions and are *** are enriched in large ion lithophile elements(e.g.K,Rb,U and Th),light REE and depleted in high-field strength elements(***,P and Ti).Depletions in Eu,Ba,Sr,Nb,Ta,P and Ti suggest that they have experienced strong fractional crystallization of plagioclase,biotite,hornblende and accessory *** pre-ore monzogranite(-172 Ma) also belongs to the high-K calc-alkaline *** fractionated REE patterns((La/Yb)N = 19.6-21.7),high values of Sr/Y(54-69) and La/Yb(29-32),are adakite-like geochemical *** post-ore rocks(-141-128 Ma) have similar geochemical characteristics with ore-forming porphyries except that quartz monzonite porphyry shows no Ba-Sr negative *** intrusive rocks have relative low initial Sr/Sr(0.705413-0.707889) and εNd(t) values(-1.28 to +0.92),positiveεHf(t) values(+ 2.4 to +10.1) and young two-stage Nd and Hf model ages(T(Nd) = 863-977 Ma,T(Hf) = 552-976 Ma).These geochemical and isotopic data are interpreted to demonstrate that the ore-forming porphyries formed by partial melting of the juvenile lower crust caused by underplating of mafic magmas in an intra-plate extensional *** pre-ore monzogranite formed by partial melting of thickened lower crust in a collisional

关键词： Chalukou porphyry Molybdenum deposit Zircon U-Pb age dating Juvenile lower crust Extensional setting Sr-Nd isotope Zircon Hf isotope

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Open Journal of Nursing 2019年第5期9卷 481-493页

作者： Kyoko Asazawa Ai Inamoto Misaki Suzuki Yukari Ashino Yurina ishihara Yumu Oba Maki Endo Ayumi Iwata Miki Kishioka Arisa Tokunaga Miharu Sumino Natsuko Kojima Eriko Shinohara Division of Nursing Faculty of Nursing Tokyo Healthcare University Tokyo Japan

Background: This study aimed to identify the predictors of LGBT recognition by health sciences university students in Japan. Methods: This is a cross-sectional study that used quantitative data collected from 481 returned self-report questionnaires distributed to 866 health sciences undergraduate and graduate students. The following survey item and scales were used for measuring the main outcomes: Thoughts about sexual identity, Empathy scale, Objectivity scale, and LGBT recognition scale. Data were analyzed using descriptive statistics, two-sample t-test, one-way analysis of variance, and multiple regression analyses. SPSS ver. 23.0 (SPSS, Chicago, IL, USA) was used for data analysis at a 5% significance level. Results: The number of returned questionnaires was 481 (55.5%). There was no significant difference in the LGBT recognition and the participant’s characteristics (e.g., age and medical history). The 5 significant predictors of LGBT recognition were: 1) Empathy (β = 0.19, p LGBT learning experience (β = 0.18, p Objectivity (β = 0.15, p Sexual problem with a close person (β = 0.13, p Suffering from gender identity (β = 0.09, p Conclusions: The predictive factors of LGBT recognition were Empathy, LGBT learning experience, Objectivity, Sexual problem with a close person, and Suffering from gender identity. Careful development and implementation of LGBT educational programs are needed to better understand the situations and ideas of LGBT parties to enhance their recognition.

关键词： Sexual and Gender Minorities Recognition Students Health Occupations Cross-Sectional Studies

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苏门答腊地区地壳的地磁影象

世界地震译丛 2005年第1期36卷 13-15页

作者： M．Purucker T．ishihara 左玉玲郑需要不详中国地震局地球物理研究所

2004年12月26日苏门答腊特大地震和海啸地区周边的地磁影象揭示了该地区现时及历史的俯冲概况。准静态远场影象(图1a)清晰地显示出欧亚和印度澳大利亚板块之间的挤压边界。沿断层破裂带，磁化地壳厚度呈正东向和北东向显著增大。该岛弧... 详细信息

2004年12月26日苏门答腊特大地震和海啸地区周边的地磁影象揭示了该地区现时及历史的俯冲概况。准静态远场影象(图1a)清晰地显示出欧亚和印度澳大利亚板块之间的挤压边界。沿断层破裂带，磁化地壳厚度呈正东向和北东向显著增大。该岛弧与俯冲板块都是磁性的，

关键词：苏门答腊地区地壳地磁影象俯冲带地震影象

endoscopic ultrasonography-guided biliary drainage:Who,when,which,and how?

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World Journal of Gastroenterology 2016年第3期22卷 1297-1303页

作者： Kazuo Hara Kenji Yamao Nobumasa Mizuno Susumu Hijioka Hiroshi Imaoka Masahiro Tajika Tutomu Tanaka Makoto ishihara Nozomi Okuno Nobuhiro Hieda Tukasa Yoshida Yasumasa Niwa Department of Gastroenterology Aichi Cancer Center HospitalNagoya 464-8681Japan Department of Endoscopy Aichi Cancer Center HospitalNagoya 464-8681Japan

Both endoscopic ultrasonography(EUS)-guided choledochoduodenostomy( EUS- CDS) and EUS-guided hepaticogastrostomy(EUS-HGS) are relatively well established as alternatives to percutaneous transhepatic biliary drainage(PTBD). Both EUSCDS and EUS-HGS have high technical and clinical success rates(more than 90%) in high-volume centers. Complications for both procedures remain high at 10%-30%. Procedures performed by endoscopists who have done fewer than 20 cases sometimes result in severe or fatal complications. When learning EUSguided biliary drainage(EUS-BD), we recommend a mentor's supervision during at least the first 20 cases. For inoperable malignant lower biliary obstruction, a skillful endoscopist should perform EUS-BD before EUS-guided rendezvous technique(EUS-RV) and PTBD. We should be select EUS-BD for patients having altered anatomy from malignant tumors before balloon-enteroscope-assisted endoscopic retrograde cholangiopancreatography, EUS-RV, and PTBD. If both EUS-CDS and EUS-HGS are available, we should select EUS-CDS, according to published data. EUSBD will potentially become a first-line biliary drainage procedure in the near future.

关键词： endoscopic ultrasonography endoscopicultrasonography-guided biliary drainage Interventionalendoscopic ultrasonography endoscopic ultrasonography-guided choledochoduodenostomy endoscopicultrasonography-guided rendezvous technique

早期肢端原位黑素瘤:皮肤镜下平行皮纹模式和显微镜下特征的关系

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世界核心医学期刊文摘（皮肤病学分册） 2006年第12期2卷 41-42页

作者： ishihara Y. Saida T. Miyazaki A. 朱国兴 Department of Dermatology Shinshu University School of Medicine 3-1-1Asahi Matsumoto 390-8621 Japan Dr.

In non-white populations, acral skin is the most prevalent site of malignant melanoma. Early melanomas of this anatomic site are often misdiagnosed as melanocytic nevi, which are not uncommon on acral skin. In fact, clinical and/or histopathological features of melanocytic nevi occasionally mimic those of early acral melanoma and vice versa, and thus differentiation of early acral melanoma from melanocytic nevus is sometimes very difficult for clinicians as well as for histopathologists. Our dermoscopic investigation has revealed that the parallel ridge pattern, a band-like pigmentation on the ridges of the skin markings, is highly specific to malignant melanoma in situ on acral volar skin. In the present study, we reviewed 22 acral melanocytic lesions that showed the parallel ridge pattern on dermoscopy but had very subtle clinical and/or histopathological presentations. We diagnosed 20 of them as early melanoma in situ by careful histopathological examination,which revealed histopathological features very similar to those seen in macular portions of overt acral melanoma, but fundamentally different from features found in melanocytic nevi on acral skin. In correspondence with their dermoscopic pattern, in these early lesions of acral melanomas, proliferation of solitary arranged melanocytes was mainly detected in the crista profunda intermedia, the epidermal rete ridge underlying the ridge of the skin marking. The two remaining lesions were diagnosed as possible cases of acquired melanocytic nevus because of the formation of well-demarcated nests of melanocytes in the epidermal rete ridges. We propose that a finding of preferential proliferation of solitary arranged melanocytes in the crista profunda intermedia is an important clue for the histopathological diagnosis of early phases of acral melanoma.

关键词：皮肤镜肢端黑素瘤黑素细胞痣皮纹

肝-豆状核变性综合征患儿肠神经丛无神经节段长度的变异

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世界核心医学期刊文摘（儿科学分册） 2006年第2期2卷 44-45页

作者： ishihara N. Shimada A. Kato J. 王经纬 Department of Pathology Institute for Developmental Research Aichi 480-0392 Japan

Background/Purpose: Patients with zinc finger homeo box 1B (ZFHX1B) mutations or deletions develop multiple congenital anomalies including Hirschsprung disease, known as Mowat-Wilson syndrome (MWS). In this study, we investigated variations in the enteric neural plexus abnormalities in MWS using morphometry-based histopathologic analysis. Methods: Seven patients with MWS (3 with mutations in exon 8 of ZFHX1B and 4 with deletions) who had undergone modified Duhamel’ s operations for Hirschsprung disease were examined. Surgically resected rectosigmoid specimens were analyzed morphometrically. Results: The length of the aganglionic segment was longer than 3 cm in all the patients with deletions. In 3 patients with mutations, the aganglionic region was not detected in the surgically resected specimens; however, the parameters of the ganglions and plexus were significantly smaller than those of controls (cloaca and aproctia), indicative of a transitional zone. Variation in the severity of pathological changes among the 3 patients with mutations was also noted. Conclusions: The variations in myenteric plexus pathologies in MWS appear to be caused by both variations in ZFHX1B abnormalities and epigenetic factors.

关键词：神经节段肠神经丛综合征豆状核患儿变异长度变性先天性巨结肠症手术切除标本