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7th World Congress on Recovery, Recycling and Re-integration

作者： Md.Asad-ud Doullah keiichi okazaki M.Bahadur Meah Faculty of Agriculture Niigata University 2-8050 Ikarashi Niigata 950-2181 Japan Faculty of Agriculture Niigata University 2-8050 Ikarashi Niigata 950-2181 Japan Department of Plant Pathology Bangladesh Agricultural University Mymensingh-2202 Bangladesh

Alternaria leaf spot is a severe disease of Brassica world wide No resistant cultivars have been found in cultivated 8. rapa till now. In this study we tried to find out resistance in B. rapa cultivars. Two hundred and seventy two B. rapa (***) of vegetable including two oil seed cultivars (B. napus and B. juncea) were screened for resistance to dark leaf spot using two isolates of A brassicicola with two different screening methods in two different seasons. Different level of resistance and susceptibility were found among the cultivars used of which 22 were found to be resistant in winter and 3 were in spring season Seasonal variation was significantly different among the cultivars tested. There were no differences between the two isolates according to their morphological characteristics and host reaction Two different screening methods used in this study were also significantly correlated. This investigation represents the first vast screening of B. rapa against A brassicicola. The source of resistance would be of interest in breeding programmes for their resistance.

关键词： Alternaria brassicicola, dark leaf spot, resistant, Brassica rapa

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Comparison of Positions of QTLs Conferring Resistance to Xanthomonas campestrispv. campestrisin Brassica oleracea

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American Journal of Plant Sciences 2013年第8期4卷 11-20页

作者： Nazmoon Naher Tonu Md. Asad-ud Doullah Motoki Shimizu Md. Masud Karim Takahiro Kawanabe Ryo Fujimoto keiichi okazaki Niigata University Niigata Japan

Black rot, caused by Xanthomonas campestris pv. campestris (Xcc) is possibly the most important disease of Brassica worldwide. To compare chromosomal positions of Xcc resistance loci in Brassica oleracea between the present and published studies and to develop marker assisted selection (MAS) to resistance against Xcc race 1, we constructed a B. oleracea map, including pW, pX and BoCL markers that were closely linked to previously reported Xcc resistance QTLs. We also analyzed Xcc resistance QTLs by improving our previously reported map derived from the cross of a susceptible double-haploid line (GC P09) with a resistant double-haploid line (Reiho P01). In the nine linkage groups obtained (C1-C9), the major QTL, XccBo(Reiho)2, was derived from Reiho with a maximum LOD score (7.7) in C8. The QTL (LOD 4.4) located in C9, XccBo(GC)1 was derived from the susceptible GC. The other QTL (LOD 4.4), XccBo(Reiho)1, was found in C5. Based on common markers, it was possible to compare our finding Xcc resistance QTLs with the B. oleraceaXcc loci reported by previous authors;XccBo(Reiho)1 and XccBo(GC)1 may be identical to the Xcc resistance QTLs reported previously or a different member contained in the same resistance gene cluster. Our map includes public SSR markers linked to Xcc resistance genes that will promote pyramiding Xcc resistance genes in B. oleracea. The present study will also contribute to a better understanding of genetic control of Xcc resistance.

关键词： Black Rot Disease Resistance QTL Xanthomonas campestris pv. campestris

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