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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2022年第1期23卷 84-88页

作者： Feifei LI kehai liu Kewu liu College of Food Science and Technology Shanghai Ocean UniversityShanghai201306China Shanghai Engineering Research Center of Aquatic-Product Processing&Preservation Shanghai201306China Heilongjiang Forest By-product and Speciality Institute Mudanjiang157011China

This study aimed to characterize the chemical composition of a new sulfated polysaccharide from the red alga Gracilaria chouae and evaluate its activation effects on RAW264.7 macrophages.It showed that the obtained G.... 详细信息

关键词： activation RAW264.7 characterization

The Rise of Two‑Dimensional‑Material‑Based Filters for Airborne Particulate Matter Removal

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Advanced Fiber Materials 2023年第2期5卷 461-483页

作者： Jun liu Enze Tian Shaolin Zhang Deyu Kong kehai liu Xuedong Bai Kaihui liu School of Physics and Materials Science Guangzhou UniversityGuangzhou 510006China Research Center for Advanced Information Materials(CAIM) Huangpu Research and Graduate School of Guangzhou UniversityGuangzhou 510555China Songshan Lake Materials Laboratory Dongguan 523808China Institute of Physics Chinese Academy of SciencesBeijing 100190China State Key Laboratory for Mesoscopic Physics School of PhysicsPeking UniversityBeijing 100871China

Airborne particulate matter(PM)has been the leading contributor to air pollution,posing a substantial risk to human health,and effective filtration technologies are required.Two-dimensional(2D)materials,such as graphene,graphitic carbon nitride(g-C3N4),molybdenum disulfide(MoS2),and MXenes have emerged in recent years for PM filtration due to their exception-ally large specific surface area and unique electrical properties.Here,the most extensively used 2D materials for PM filtration followed by a summary of their fabrication methods and corresponding morphologies were introduced.Among them,the coating is the most cost-effective technique for achieving large-scale and quick fabrication.Electrospinning can effectively enhance filtration efficiency and reduce pressure drop by upgrading electrostatic force and lowering the fiber diameter.The initial and long-term performance of 2D-material-based filters was summarized.Among all 2D materials,GO is the most studied and shows the best performance by upgrading the dipole–dipole and electrostatic interaction between filters and PM.Future study is expected to explore various 2D materials beyond GO,to evaluate filtration efficiency for submicron PM at m/s level air velocity,and to extend the service life for potential applications.

关键词： Two-dimensional material Graphene PM filtration Fabrication Electrospinning Coating

Abnormal anti-oxidation behavior of hexagonal boron nitride grown on copper

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Nano Research 2022年第8期15卷 7577-7583页

作者： Li Wang Jiajie Qi Shuai Zhang Mingchao Ding Wei Wei Jinhuan Wang Zhihong Zhang Ruixi Qiao Zhibin Zhang Zehui Li kehai liu Ying Fu Hao Hong Can liu Muhong Wu Wenlong Wang Jun He Yi Cui Qunyang Li Xuedong Bai Kaihui liu State Key Laboratory for Mesoscopic Physics Frontiers Science Center for Nano-optoelectronicsSchool of PhysicsPeking UniversityBeijing 100871China Beijing National Laboratory for Condensed Matter Physics Institute of PhysicsChinese Academy of SciencesBeijing 100190China Department of Engineering Mechanics State Key Laboratory of TribologyTsinghua UniversityBeijing 100084China Vacuum Interconnected Nanotech Workstation Suzhou Institute of Nano-Tech and Nano-BionicsChinese Academy of SciencesSuzhou 215123China School of Chemistry and Chemical Engineering Beijing Institute of TechnologyBeijing 100081China International Centre for Quantum Materials Collaborative Innovation Centre of Quantum MatterPeking UniversityBeijing 100871China Songshan Lake Materials Laboratory Institute of PhysicsChinese Academy of SciencesDongguan 523808China CAS Center for Excellence in Nanoscience CAS Key Laboratory of Nanosystem and Hierarchical FabricationNational Center for Nanoscience and TechnologyBeijing 100190China School of Physical Sciences University of Chinese Academy of SciencesBeijing 100049China

Atomic-layered hexagonal boron nitride(hBN)is expected to be the best two-dimensional(2D)anti-oxidation layer on metals for its incomparable impermeability,insulativity,and stability,as well as the progressive bottom-up growth techniques to ensure fast coating on metal surface in large area.However,its real anti-oxidation ability in practice is found to be unsatisfactory and nonuniform,and the main obstacle to achieving ideal anti-oxidation performance lies in unclear anti-oxidation behavior at special interface between 2D hBN and three-dimensional(3D)metals.Herein,system of monolayer hBN grown on copper(Cu)foils with various lattice orientations was grown to investigate the anti-oxidation behavior of different interlayer configurations.By using structural characterizations together with analysis of topography,we surprisingly found that stronger interlayer coupling led to worse anti-oxidation performance owing to fast diffusion of O2 through higher hBN corrugations generated at the commensurate hBN/Cu(111)configuration.In view of this,we developed the approach of cyclic reannealing that can effectively flatten corrugations and steps,and therefore improve the anti-oxidation performance to a great extent.This work provides a more indepth understanding of anti-oxidation behavior of 2D materials grown on 3D metals,and a practical method to pave the way for its large-scale applications in future.

关键词： hexagonal boron nitride anti-oxidation of metals nanoscale corrugation cyclic reannealing method

Multi-Mode Guided Waves Based Reference-Free Damage Diagnostic Imaging in Plates

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Structural Durability & Health Monitoring 2019年第1期13卷 41-59页

作者： Jiaqi Zhang kehai liu Chang Gao Zhanjun Wu Yuebin Zheng Dongyue Gao State Key Laboratory of Structural Analysis for Industrial Equipment Dalian University of TechnologyDalian116024China School of Civil Engineering Dalian University of TechnologyDalian116024China School of Aeronautics and Astronautics Sun Yat-sen UniversityGuangzhou510275China

Probability-based diagnostic imaging(PDI)is one of the most well-known damage identification methods using guided waves.It is usually applied to diagnose damage in plates.The previous studies were dependent on the certain damage index(DI)which is always calculated from the guided wave signals.In conventional methods,DI is simply defined by comparing the real-time data with the baseline data as reference.However,the baseline signal is easily affected by varying environmental conditions of structures.In this paper,a reference-free diagnostic imaging method is developed to avoid the influence of environmental factors,such as temperature and load conditions.The DI is defined based on the mode conversion of multi-mode guided waves with realtime signals without baseline signals.To improve the accuracy of diagnosis,two terms are included in the reference-free DI.One is called energy DI,which is defined based on the feature of signal energy.The other is called correlation DI and is defined based on the correlation coefficient.Then the PDI algorithm can be carried out instantaneously according to the reference-free DI.The real-time signals which are used to calculate DI are collected by the piezoelectric lead zirconate titanate(PZT)transducers placed on both sides of a plate.The numerical simulations by the finite element(FE)method on aluminum plates with PZT arrays are performed to validate the effectiveness of the reference-free damage diagnostic imaging.The approach is validated by two different arrays:a circle network and a square network.The results of diagnostic imaging are demonstrated and discussed in this paper.Furthermore,the advantage of reference-free DI is investigated by comparing the accuracy of defined reference-free DI and energy DI.

关键词： Multi-mode guided waves probability-based diagnostic imaging referencefree mode conversion

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海马多糖提取工艺优化与抗氧化活性研究

上海海洋大学学报 2022年第5期31卷 1295-1303页

作者：王玉刘克海上海海洋大学食品学院上海201306 上海水产品加工及贮藏工程技术研究中心上海201306

以日本海马(Hippocampus mohnikei)为原料,采用响应面试验优化超声辅助提取粗多糖工艺条件,通过柱层析进行纯化,测定海马多糖的单糖组成,并评价其抗氧化活性。结果表明:超声时间47 min、浸提温度87℃、液料比23 mL/g为最佳提取条件,粗多... 详细信息

以日本海马(Hippocampus mohnikei)为原料,采用响应面试验优化超声辅助提取粗多糖工艺条件,通过柱层析进行纯化,测定海马多糖的单糖组成,并评价其抗氧化活性。结果表明:超声时间47 min、浸提温度87℃、液料比23 mL/g为最佳提取条件,粗多糖HP得率为9.91%;通过柱色谱分级纯化得到2种多糖HP-1和HP-2,HP-1主要由葡萄糖、半乳糖、甘露糖以摩尔比11.32∶6.39∶5.64组成,并含有少量阿拉伯糖、木糖、岩藻糖、鼠李糖,HP-2主要由氨基葡萄糖、葡萄糖醛酸、半乳糖醛酸、半乳糖、葡萄糖、甘露糖以摩尔比29.03∶18.18∶8.48∶6.30∶4.88∶2.70组成,并含有少量岩藻糖和木糖;HP-1、HP-2具有良好DPPH自由基清除能力,且HP-2最强,同时HP、HP-1及HP-2均具有还原力,表明海马多糖具有良好抗氧化活性。

关键词：日本海马多糖超声提取响应面优化单糖组成抗氧化

甘草次酸和三苯基膦共介导多柔比星线粒体靶向的体外评价

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中国医药工业杂志 2023年第4期54卷 562-568页

作者：陈慧婷刘克海上海海洋大学食品学院上海201306

该研究以甘草次酸(GA)-多柔比星(1)-三苯基膦(TPP)为模型药,采用薄膜分散法制备透明质酸(HA)修饰的阳离子脂质体,构建了线粒体靶向递送系统(LP-G1T@HA),并评价了其制剂学相关性质及体外抗肿瘤活性。理化性质分析结果显示,LP-G1T@HA粒径... 详细信息

该研究以甘草次酸(GA)-多柔比星(1)-三苯基膦(TPP)为模型药,采用薄膜分散法制备透明质酸(HA)修饰的阳离子脂质体,构建了线粒体靶向递送系统(LP-G1T@HA),并评价了其制剂学相关性质及体外抗肿瘤活性。理化性质分析结果显示,LP-G1T@HA粒径为(340.3±2.3)nm,包封率为(85.4±3.1)%,体外血清稳定性良好,溶血毒性低。体外细胞试验结果显示,LP-G1T@HA对人乳腺癌细胞(MCF-7细胞)增殖有明显的抑制作用,同时可诱导线粒体膜通透性转换孔开放,加速细胞凋亡,说明LP-G1T@HA可作为有效的线粒体靶向递送系统,实现高效抗肿瘤作用。

关键词：多柔比星甘草次酸线粒体靶向透明质酸阳离子脂质体抗肿瘤活性

水芹多糖的提取及其对巨噬细胞RAW264.7免疫活性的初步研究

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食品与发酵工业 2023年第6期49卷 79-85页

作者：程雷崔明晓刘可玉刘克海上海海洋大学食品学院上海201306 上海水产品加工及贮藏工程技术研究中心上海201306

多糖是水芹中重要功能性成分,其分离和活性的相关研究尚未报道。该研究基于超声波辅助法从水芹中成功分离获得功能性多糖成分——水芹多糖(Oenanthe javanica polysaccharide,OJP),利用响应面法优化了OJP的分离条件,并考察了OJP对巨噬细... 详细信息

多糖是水芹中重要功能性成分,其分离和活性的相关研究尚未报道。该研究基于超声波辅助法从水芹中成功分离获得功能性多糖成分——水芹多糖(Oenanthe javanica polysaccharide,OJP),利用响应面法优化了OJP的分离条件,并考察了OJP对巨噬细胞RAW264.7的激活作用。响应面优化结果显示,OJP最佳分离条件为超声温度83.6℃、超声时间51 min、料液比1∶29.7(g∶mL),提取率为13.81%。单糖组成结果显示OJP由阿拉伯糖(29.94%)、半乳糖(52.48%)、葡萄糖(11.32%)以及微量木糖(2.22%)、半乳糖醛酸(3.5%)和葡萄糖醛酸(0.53%)组成。细胞实验结果表明,OJP可促进RAW264.7巨噬细胞增殖及NO产生并增强RAW264.7细胞的吞噬能力。该研究为OJP的开发与应用提供了实验基础。

关键词：水芹多糖响应面法单糖组成巨噬细胞RAW264.7

枸骨果多糖的分离纯化和单糖分析及抗氧化特性

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甘肃农业大学学报 2022年第2期57卷 192-200页

作者：田俊雅刘克海上海海洋大学食品学院上海201306 上海水产品加工及贮藏工程技术研究中心上海201306

【目的】优化枸骨果多糖超声辅助提取工艺条件,分离、纯化粗多糖,得到均一多糖后对其单糖组成和抗氧化能力进行测定。【方法】根据单因素试验结果,利用Design Expert软件设计正交试验优化了枸骨果多糖的提取工艺。粗多糖经三氯乙酸(tric... 详细信息

【目的】优化枸骨果多糖超声辅助提取工艺条件,分离、纯化粗多糖,得到均一多糖后对其单糖组成和抗氧化能力进行测定。【方法】根据单因素试验结果,利用Design Expert软件设计正交试验优化了枸骨果多糖的提取工艺。粗多糖经三氯乙酸(trichloroacetic acid,TCA)法除蛋白后,使用柱层析分级纯化,得到均一多糖LCFP-3。通过高效离子色谱法测定LCFP-3的单糖组成,通过DPPH自由基清除能力及总还原力的测定,评估LCFP-3的抗氧化能力。【结果】最佳工艺条件:超声时间、超声温度、液料比分别以53 min、63℃、26 mL∶g,此条件下多糖得率为2.36%。LCFP-3的总糖含量为90.31%,蛋白含量为0.246%,分子量为41199 U,主要由半乳糖(31.92%)、阿拉伯糖(25.87%)、半乳糖醛酸(23.35%)组成,并含有少量鼠李糖、葡萄糖、甘露糖和木糖。LCFP-3质量浓度为50 mg/mL时对DPPH自由基的清除率为79.0%,质量浓度在3~25 mg/mL范围内与还原力有良好的线性关系。【结论】从枸骨果中提取纯化了一种多糖LCFP-3,对其化学性质进行分析表明其具有抗氧化活性。

关键词：枸骨果超声辅助提取多糖纯化抗氧化

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无需先验信息的两步项目功能差异检验方法

心理科学 2024年第3期47卷 734-743页

作者：韩雨婷袁克海刘红云北京语言大学心理学院北京100083 南京邮电大学理学院南京210023 美国圣母大学心理系印第安纳州46556 北京师范大学心理学部北京100875 应用实验心理北京市重点实验室心理学国家级实验教学示范中心(北京师范大学)北京100875

传统的项目功能差异检验方法依赖先验信息设定锚题,误设锚题可能产生误导性结果。研究提出以数据驱动的难度差异QQ图(D-QQ图)选择锚题,再结合传统方法检验DIF的两步DIF检验法。两个实证研究说明了新方法在实际测验公平性检验中的适用性... 详细信息

传统的项目功能差异检验方法依赖先验信息设定锚题,误设锚题可能产生误导性结果。研究提出以数据驱动的难度差异QQ图(D-QQ图)选择锚题,再结合传统方法检验DIF的两步DIF检验法。两个实证研究说明了新方法在实际测验公平性检验中的适用性及可视化优势。模拟研究进一步表明当测验中有一半试题存在DIF时,若DIF试题仅偏向一组,则两步法兼具高统计检验力和低I类错误的优势;若DIF试题分别有利于两组,则其在I类错误控制上优于RCD方法。

关键词：项目功能差异 D-QQ图图形检验两步DIF检验法

MMP-2响应的多肽修饰的PAMAM纳米颗粒负载DOX在乳腺癌细胞中的效应

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广州医药 2023年第10期54卷 12-19页

作者：顼嘉琪刘克海上海海洋大学食品学院上海201306

目的构建尺寸可变纳米递送系统PAMAM/DOX-pep并进行表征,检测其理化性质并评价其体外抗肿瘤效果与靶向性。方法将阿霉素(DOX)物理包埋在阳离子聚合物PAMAM的疏水空腔内,以4-(N-马来酰亚胺基甲基)环己烷羧酸N-羟基琥珀酰亚胺酯(SMCC)作... 详细信息

目的构建尺寸可变纳米递送系统PAMAM/DOX-pep并进行表征,检测其理化性质并评价其体外抗肿瘤效果与靶向性。方法将阿霉素(DOX)物理包埋在阳离子聚合物PAMAM的疏水空腔内,以4-(N-马来酰亚胺基甲基)环己烷羧酸N-羟基琥珀酰亚胺酯(SMCC)作为交联剂,采用金属基质蛋白酶(MMP-2)敏感的多肽pep(CPLGVRGC)串联小粒径纳米颗粒形成大尺寸纳米递送系统(PAMAM/DOX-pep),对各纳米颗粒的粒径、电位、理化性质以及对小鼠乳腺癌细胞(4T1)的抑制作用、细胞摄取效果和核靶向作用进行检测。结果PAMAM/DOX粒径约为10 nm,载药率为23%,多肽pep交联后形成的PAMAM/DOX-pep粒径约为200 nm,可在低pH下缓释DOX,7天内体外保持稳定且溶血率低、安全无毒,其与MMP-2共孵育后细胞摄取量与核靶向性显著增加。结论尺寸可变纳米颗粒有助于克服尺寸所引发的递送障碍,将药物靶向递送至乳腺癌细胞核内并发挥作用,为纳米递送系统的设计提供了新策略。

关键词：尺寸可变纳米递送系统多肽肿瘤微环境响应