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国际生物制品学杂志 1984年第2期07卷 67-67页

作者： keese cr 董之昌

1967年van Wezel介绍了贴壁依赖细胞在小圆珠上生长成单层的微载体培养方法。这种系统能在小体积中提供相当大的表面积、可以有效地利用培养基,从而促进细胞大量生长。首先使用的是DEAE-Sephadex

关键词：聚赖氨酸 FC 氟化碳血清蛋白质细胞层微载体

Abdominal cocoon in children: A case report and review of literature

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World Journal of Gastroenterology 2021年第37期27卷 6332-6344页

作者： Daniel keese Andrea Schmedding Kerstin Saalabian Georgy Lakshin Henning Fiegel Udo Rolle Goethe-University Frankfurt Department of Paediatric Surgery and Paediatric UrologyUniversity HospitalFrankfurt 60590Germany

BACKGROUND Abdominal cocoon or“encapsulating peritoneal sclerosis”(EPS)is an uncommon and rare cause of intestinal *** a few cases have been reported in paediatric ***,EPS is described as the primary form in young adolescent girls from tropical and subtropical countries because of viral peritonitis due to retrograde menstruation or a history of peritoneal *** patients are asymptomatic or present with abdominal pain,which is likely to occur secondary to subacute bowel *** at imaging,such as ultrasound,computed tomography,and magnetic resonance imaging,are often *** diagnosed,EPS is characterized by total or partial encasement of the bowel within a thick fibrocollagenous membrane that envelopes the small intestine in the form of a cocoon because of chronic intraabdominal fibroinflammatory *** membrane forms a fibrous tissue sheet that covers,fixes,and finely constricts the gut,compromising its *** SUMMARY We present a case of EPS in a 12-year-old boy 8 wk after primary surgery for resection of symptomatic jejunal *** was no history of peritoneal dialysis or drug *** In this report,we sought to highlight the diagnostic,surgical,and histopathological characteristics and review the current literature on EPS in paediatric patients.

关键词： Abdominal cocoon Peritoneal encapsulation Encapsulating peritoneal sclerosis Intestinal obstruction Children Case report

Chronic mesenteric ischemia: Time to remember open revascularization

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World Journal of Gastroenterology 2013年第9期19卷 1333-1337页

作者： Michael keese Thomas Schmitz-Rixen Thomas Schmandra Clinic for Vascular and Endovascular Surgery Johann Wolfgang Goethe University Hospital 60590 Frankfurt Germany

Chronic mesenteric ischemia is caused by stenosis or occlusion of one or more visceral arteries. It represents a therapeutic challenge and diagnosis and treatment require close interdisciplinary cooperation between gastroenterologist, vascular surgeon and radiologist. Although endovascular treatment modalities have been developed, the number of restenoses ultimately resulting in treatment failure is high. In patients fit for open surgery, the visceral arteries should be revascularized conventionally. These patients will then experience long term relief from the symptoms, a better quality of life and a better overall survival.

关键词： Chronic mesenteric ischemia Stent Vascular surgery Restenosis Prognosis

血管内皮生长因子936T/C基因多态性与结直肠癌及术后吻合口瘘的关系

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中华外科杂志 2006年第21期44卷 1505-1507页

作者：吴国洋王效民 Michael keese Till Hasenberg Jrg W. Sturm 厦门大学附属中山医院普外科 361004 德国海德堡大学医学院曼海姆医院普通外科

目的通过研究血管内皮生长因子(VEGF)936T/C基因多态性了解该因子对结直肠癌生成及术后结肠吻合口瘘的影响。方法采用聚合酶链反应-限制性片段长度多态性(Pcr-RFLP)方法检测结直肠癌及正常对照的VEGF936T/C基因型。结果结直肠癌患者VEGF... 详细信息

目的通过研究血管内皮生长因子(VEGF)936T/C基因多态性了解该因子对结直肠癌生成及术后结肠吻合口瘘的影响。方法采用聚合酶链反应-限制性片段长度多态性(Pcr-RFLP)方法检测结直肠癌及正常对照的VEGF936T/C基因型。结果结直肠癌患者VEGF936T/C基因型或等位基因与对照组相比无差异。伴有术后结肠吻合口瘘的结直肠癌患者其VEGF936C/C基因型及C等位基因比例低于无术后结肠吻合口瘘的患者,两者差异有统计学意义(P<0·05)。结论VEGF936C/C基因型或936C等位基因与结直肠癌的发生无关,但提示术后结肠吻合口瘘的发生率降低。

关键词：内皮生长因子结肠直肠肿瘤基因多态性吻合口瘘

Extravascular use of drug-eluting beads: A promising approach in compartment-based tumor therapy

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World Journal of Gastroenterology 2013年第43期19卷 7586-7593页

作者： Simon Binder Andrew L Lewis J-Matthias Lhr Michael keese Faculty of Medicine Mannheim University of Heidelberg68167 MannheimGermany Biocompatibles UK Ltd. Department for Research and DevelopmentFarnham GU98QL SurreyUnited Kingdom Karolinska Institute Department of Clinical ScienceIntervention and Technology17177 StockholmSweden Clinic for Vascular and Endovascular Surgery University Hospital Frankfurt60590 Frankfurt am MainGermany

Intraperitoneal carcinomatosis(PC)may occur with several tumor *** prognosis of patients suffering from PC is usually *** treatment depends on the cancer entity and includes systemic chemotherapy,radiation therapy,hormonal therapy and surgical *** few patients may also benefit from hyperthermic intraperitoneal chemotherapy with a complete tumor *** therapies are often accompanied by severe systemic *** approach to reduce side effects is to target chemotherapeutic agents to the tumor with carrier *** experimental results have been achieved using drug-eluting beads(DEBs).A series of in vitro and in vitro experiments has been conducted to determine the suitability of their extravascular *** encapsulation devices were able to harbor CYP2B1producing cells and to shield them from the hosts immune system when injected *** this way ifosfamide-which is transformed into its active metabolites by CYP2B1-could be successfully targeted into pancreatic tumor *** DEBs can be used to target chemotherapeutics into the abdominal cavity for treatment of *** CYP2B1 producing cells are proven to be save for usage in man and if local toxic effects of chemotherapeutics can be controlled,DEBs will become promising tools in compartmentbased anticancer treatment.

关键词： Compartment based therapy Intraperitoneal Drug-eluting beads Carcinomatosis Hyperthermic intraperitoneal chemotherapy Glioblastoma Pancreatic cancer CYP2B1 Ifosfamide

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观赏植物育苗钵除草剂流失

环保科技情报 1996年第5期 20-26页

作者： keese,RJ

Nucleotide sequence of coat protein gene for GPV isolate of barley yellow dwarf virus and construction of expression plasmid for plant

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Science China(Life Sciences) 1996年第5期39卷 534-543页

作者：成卓敏何小源吴茂森周广和 Paul keese P.M.Waterhouse State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant Protection Chinese Academy of Agricultural Sciences Beijing 100094China Division of Plant Industry SCIRO Canberra Australia

GPV is a Chinese serotype isolate of barley yellow dwarf virus (BYDV) that has no reactionwith antiserum of MAV, PAV, SGV, RPV and RMV. The sequence of the coat protein (CP) of GPV isolate of BYDV was identified and its amino acid sequence was deduced. The coding region for the putative GPV CP is 603 bases nucleotides and encodes a Mr 22218 (22 ku) protein. The same as MAV, PAV and RPV, GPV contained a second ORF within the coat protein coding region. This protein of 17024 Mr (17 ku) is thought to correspond to the Virion protein genome linked (Vpg). Sequence comparisons of the CP coding region between the GPV isolate of BYDV and other isolates of BYDV have been done. The nucleotide and ammo acid sequence homology of GPV has a greater identity to the sequence of RPV than those of PAV and MAV. The GPV CP sequence shared 83.7% of nucleotide similarity and 77.5% of deduced amino add similarity, whereas that of the PAV and MAV shared 56.9%. 53.2% and 44.1%. 43.8% respectively. According to BYDV-GPV CP sequence, two primers were designed. The cDNA of CP was produced by RT-Pcr. Full-length cDNA of CP was inserted into plasmid to construct expression plasmids named pPPI1. pPPI2 and pPPI5 based on different promoters. The recombinant plasmids were identified by using α-32P-dATP labelled CP probe. α-32P-ATP labelled GPV RNA probe and sequencing to confirm real GPV CP gene cDNA in plasmids.

关键词： BYDV GPV CP expression plasmid.

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EGF基因多态性与结直肠癌的关系

中华普通外科杂志 2005年第11期20卷 738-739页

作者：吴国洋 Michael keese Till Hasenberg Jrg W.Sturm 厦门大学附属中山医院普外科 361004 德国海德堡大学医学院曼海姆医院普通外科

2002年Shahbazi[1]提出表皮生长因子(epidermal growth factor, EGF) 61*G/A基因多态性与皮肤黑色素瘤相关,但目前EGF 61*G/A基因多态性与其他肿瘤的研究尚未见报道.我们对EGF 61*G/A基因多态性与结直肠癌的关系进行研究,并将结果报告... 详细信息

2002年Shahbazi[1]提出表皮生长因子(epidermal growth factor, EGF) 61*G/A基因多态性与皮肤黑色素瘤相关,但目前EGF 61*G/A基因多态性与其他肿瘤的研究尚未见报道.我们对EGF 61*G/A基因多态性与结直肠癌的关系进行研究,并将结果报告如下。

关键词：基因多态性结直肠癌 EGF 表皮生长因子 factor 皮肤黑色素瘤结果报告

结直肠癌术后吻合口瘘与表皮生长因子、转化生长因子-β1、血管内皮生长因子基因多态性的关系

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中华实验外科杂志 2008年第3期25卷 296-298页

作者：吴国洋王效民 Michael keese Till Hasenberg Jǒrg W. Sturm 厦门大学附属中山医院普外科 361004 德国海德堡大学医学院曼海姆医院普通外科

目的观察表皮生长因子（EGF）61＊G／A、转化生长因子-β1（TGF-β1）-509＊TIC、血管内皮生长因子（VEGF）936＊T／C基因功能多态性对术后结肠吻合口瘘的影响。方法采用聚合酶链反应-限制性片段长度多态性（Pcr—RFLP）方法检测伴有和... 详细信息

目的观察表皮生长因子（EGF）61＊G／A、转化生长因子-β1（TGF-β1）-509＊TIC、血管内皮生长因子（VEGF）936＊T／C基因功能多态性对术后结肠吻合口瘘的影响。方法采用聚合酶链反应-限制性片段长度多态性（Pcr—RFLP）方法检测伴有和不伴有术后结肠吻合口瘘结直肠癌患者的EGF 61＊G／A，TGF-β1—509＊T／C，VEGF 936＊T／C基因型。结果伴有术后结肠吻合口瘘的结直肠癌患者TGF-β1-509＊T／T基因型比例（0．0％）低于无术后吻合口瘘的患者（15．2％），两者差异有统计学意义（P〈0．05）。伴有术后结肠吻合口瘘的VEGF 936＊C／C基因型和C等位基因比例（65．9％和80．6％）低于无术后吻合口瘘的患者（82．6％和90．5％），两者差异有统计学意义（基因型x^2=5．755，P〈0．05；等位基因x^2=5．275，P〈0．05）。伴有术后结肠吻合口瘘的结直肠癌患者EGF 61＊G／G基因型比例（25．0％）低于无术后吻合口瘘的患者（55．5％），但两者差异无统计学意义（x^2=1．591，P〉0．05）。结论TGF-β1-509＊T／T基因型，VEGF 936＊C／C基因型或936＊C等位基因有助于减少术后结肠吻合口瘘的发生。

关键词：结直肠肿瘤基因多态性吻合口瘘

TGF-β1-509 T／T基因型与结直肠癌患者术后结肠吻合口瘘的关系

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中华普通外科杂志 2007年第5期22卷 359-361页

作者：吴国洋王效民 Michael keese Till Hasenberg Jorg W. Sturm 厦门大学附属中山医院普通外科 361004 德国海德堡大学医学院曼海姆医院普通外科

目的通过研究转化生长因子-β1（TGF-β1）基因功能的多态性,了解TGF-β1因子对结直肠癌术后结肠吻合口瘘的影响。方法采用Pcr-RFLP方法检测结直肠癌组内157例伴有或不伴有术后结肠吻合口瘘患者及对照组117例的TGF-β1-509 T／C基因型... 详细信息

目的通过研究转化生长因子-β1（TGF-β1）基因功能的多态性,了解TGF-β1因子对结直肠癌术后结肠吻合口瘘的影响。方法采用Pcr-RFLP方法检测结直肠癌组内157例伴有或不伴有术后结肠吻合口瘘患者及对照组117例的TGF-β1-509 T／C基因型。结果伴有术后结肠吻合口瘘的患者TGF-β1-509 T／T。基因型比例（0）低于无吻合口瘘的患者（13．2％）,两者差异有统计学意义（P=0．012）。结直肠癌组TGF-β1-509基因型或等位基因与对照组差异无统计学意义（基因型χ^2=0．981,P〉0．05;等位基因χ^2=0．000,P〉0．05）。结论具TGF-β1-509 T／T基因型者术后结肠吻合口瘘的发生率较低。而TGF-β1-509 T/C基因多态性与结直肠癌的发生及发展无关。

关键词：结直肠肿瘤基因转化生长因子β 吻合口瘘