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Impedance Characterization of Adsorption Process of Calmodulin on Au Substrate and its Combination with Ca^(2+)

Chinese Chemical Letters 2002年第4期13卷 351-354页

作者： keqiangding HuiBoSHAO 等 InstituteofCoalChemistry ChineseAcademyofScienceTaiyuan030001 DepartmentofChemistry CapitalNormalUniversityBeijing100037

In this paper, the adsorption process of calmodulin (CaM) on Au substrate was first investigated with electrochemical impedance spectroscopy (EIS) method. The result reveals that the adsorption of the protein-calmodulin contains two steps, i.e., one short quick step followed by a slow one. The complexation of calmodulin with Ca2+ was also first probed using EIS technique, in which the complexation of CaM with Ca2+ could be reflected by the change of apparent membrane capacitance(Capp) clearly. In all above measurements, a redox couple Fe(CN)63-/ Fe(CN)64- was used as probing-pin to reflect all the changes occurring in the above process. Our work suggests that some biological processes of CaM could be studied using EIS method conveniently.

关键词： Calmodulin (CaM) adsorption behavior Au substrate complexation electrochemical impedance spectroscopy.

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Capacitance Characterization of the Effect of pH Value on the Self-assembled Monolayers of Octadecanethiol

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Chinese Chemical Letters 2003年第4期14卷 411-412页

作者： keqiangding QingFeiWANG RuTingTONG HuiBoSHAO ChemistryCollege HebeiTeachersUniversityShijiazhuang050016 DepartmentofChemistry CapitalNormalUniversityBeijing100037

In this paper, the membrane capacitance (Cm), which was obtained from the electro- chemical impedance spectroscopy (EIS) method, was used to characterize the effect of pH value on the self-assembled monolayers (SAMs) of octadecanethiol(18SH) for the first time. The results not only strongly proved that inorganic ions could penetrate the SAMs of 18SH, but also ascertained that SAMs of 18SH were not an absolute of free of ion-penetration. Verifying the existence of pin-holes in the octadecanethiol SAMs was the main contribution of this paper, which coincided with the former conjecture very well.

关键词： Octadecanethiol SAMs effect pH value capacitance characterization.

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