检索结果-南通市图书馆

Strong association of SLCIA1 and DPF3 gene variants with idiopathic male infertility in Han Chinese

Asian Journal of Andrology 2017年第4期19卷 486-492页

作者： Shu-Yuan Liu Chang-Jun Zhang Hai-Ying Peng Hao Sun ke-qin lin Xiao-qin Huang Kai Huang Jia-You Chu Zhao-qing Yang Department of Medical Genetics Institute of Medical Biology Chinese Academy of Medical Sciences and Peking Union Medical College Kunming 650118 China Reproductive Medical Research Centre People's Hospital of Shiyan Shiyan 442000 China

Male infertility is a multifactorial syndrome encompassing a wide variety of disorders. In recent years, several genome-wide single-nucleotide polymorphism （SNP） association studies （GWAS） have been performed on azoospermia and/or oligozoospermia in different populations including two GWAS on nonobstructive azoospermia in China; however, the association of SNPs with idiopathic male infertility, especially asthenozoospermia and oligozoospermia, and their correlation with semen parameters are still not clear. To investigate genetic variants associated with idiopathic male infertility （asthenozoospermia, oligozoospermia, and oligoasthenozoospermia） in Chinese Han people, 20 candidate SNPs were selected from GWAS results and genotyped using the Sequenom MassARRAY assay. A total of 136 subfertile men and 456 healthy fertile men were recruited, rs6476866 in SLCIA1 （P = 1.919E-4, OR = 0.5905, 95% Ch 0.447-0.78） and rs10129954 in DPF3 （P = 0.0023, OR = 2.199, 95% Ch 1.311-3.689） were strongly associated with idiopathic male infertility. In addition, positive associations were observed between asthenozoospermia and rs215702 in LSM5 （P= 0.0016, OR = 1.479, 95% CI： 1.075-2.033） and between oligoasthenozoospermia and rs2477686 in PEXIO （P= 0.0011, OR = 2.935, 95% Ch 1.492-5.775）. In addition, six SNPs （rs215702 in LSMS, rs6476866 in SLCIA1, rs10129954 in DPF3, rs1801133 in MTHFR, rs2477686 in PEXIO, and rs10841496 in PED3A） were significantly correlated with semen quality alterations. Our results suggest that idiopathic male infertility in different ethnic groups may share the same mechanism or pathway. Cohort expansion and further mechanistic studies on the role of genetic factors that influence spermatogenesis and sperm progressive motility are suggested.

关键词： candidate SNPs Han Chinese idiopathic male infertility semen quality

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

HLA polymorphism of the Zhuang population reflects the common HLA characteristics among Zhuang-Dong language-speaking populations

引用

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2011年第6期12卷 428-435页

作者： Li SHI Xiao-qin HUANG Lei SHI Yu-fen TAO Yu-feng YAO Liang YU ke-qin lin Wen YI Hao SUN Katsushi TOKUNAGA Jia-you CHU Institute of Medical Biology Chinese Academy of Medical Sciences & Peking Union Medical College Kunming 650118 China Department of Human Genetics Graduate School of Medicine University of Tokyo Tokyo 113-0033 Japan Department of Human Genetics Graduate School of Medicine University of Tokyo Tokyo 113-0033 Japan Department of Human Genetics Graduate School of Medicine University of Tokyo Tokyo 113-0033 Japan

A study of the human leukocyte antigen （HLA） genetic characteristics in the Zhuang, the largest ethnic population in China, would provide insight into Zhuang history and give a useful tool for disease associations, transplantation, and anthropology. In the present study, we report the comprehensive HLA-A, HLA-B, HLA-C, and HLA-DRB1 alleles and haplotypes in the Zhuang population of southern China for the first time. A total of 13 HLA-A, 24 HLA-B, 22 HLA-C, and 18 HLA-DRB1 were identified in 104 Zhuang individuals. The frequencies of HLA-A＊11：01, A^＊02：07, A^＊24：02, A^＊02：03, and A^＊33：03 on A loci, B^＊15：02, B^＊58：01, B^＊46：01, and B^＊13：01 on B loci, C^＊03：04, C^＊08：01, C^＊01：02, C^＊03：02, and C^＊07：02 on C loci, and DRBl＊15：01, DRBl^＊16：02, DRB1^＊14：01, DRBl^＊15：02, and DRBI^＊03：01 on the DRB1 loci were 〉10%. The A^＊33：03-C^＊03：02-B^＊58：01-DRBl^＊03：01 and A^＊02：07-C^＊01：02- B^＊46：01-DRB1^＊14：01 haplotypes were predominant in the Zhuang. The phylogenetic tree, as well as the analysis of haplotypes, suggested that the Zhuang are genetically similar to southern Chinese populations, especially the Zhuang-Dong language-speaking populations, such as the Bouyei, Dai, and Maonan. Even though the Zhuang and southern Chinese populations shared common alleles and haplotypes, the Zhuang has maintained its unique genetic characteristics.

关键词： Human leukocyte antigen (HLA) alleles Haplotypes Zhuang population

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Positive Selection of CAG Repeats of the ATXN2 Gene in Chinese Ethnic Groups

引用

Journal of Genetics and Genomics 2013年第10期40卷 543-548页

作者： Xiao-Chen Chen Hao Sun Chang-Jun Zhang Ying Zhang ke-qin lin Liang Yu Lei Shi Yu-Fen Tao Xiao-qin Huang Jia-You Chu Zhao-qing Yang Department of Medical Genetics Institute of Medical BiologyChinese Academy of Medical Sciences and Peking Union Medical College935 Jiaoling RoadKunming 650118YunnanChina Reproductive Medicine Center Hubei University of MedicineShiyan 442000China

The ataxin-2 （ATXN2） gene is located on human chromo-some 12q24.1. In normal individuals, the coding region in exon 1 of this gene has fewer than 31 CAG repeats （Yu et al., 2005： Laffita-Mesa et al., 2012）. However, an abnormal expansion of CAG trinucleotide repeats results in the aggre-gation of polyglutamine （polyQ）, which causes spinocer-ebellar ataxia type 2 （SCA2）（Pulst et al., 1996）. The expanded alleles have more than 32 repeats in the affected individuals, and generally there is an inverse correlation between CAG repeat length and age of onset （Pulst et al., 1996）. SCA2 is an autosomal dominant inheritance neurodegenerative disease, whose major clinical feature is progressive cerebellar ataxia. Atrophies of the brainstem and frontal lobe have been frequently detected by magnetic resonance imaging （MRI）（Yamamoto-Watanabe et al., 2010）. This disease has the strong effect on sensory and motor control.

关键词： CAG Gene Positive Selection of CAG Repeats of the ATXN2 Gene in Chinese Ethnic Groups

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

DNA Vaccine of SARS-Cov S Gene Induces Antibody Response in Mice

引用

Acta Biochimica et Biophysica Sinica 2004年第1期36卷 37-41页

作者： Ping ZHAO, Jin-Shan ke, Zhao-lin qin, Hao REN, Lan-Juan ZHAO, Jian-Guo YU, Jun GAO, Shi-Ying ZHU, and Zhong-Tian QI (Department of Microbiology, Second Military Medical University, Shanghai 200433, China Department of Infectious Diseases, PLA Hospital No.88, Tai'an 271000, China ) Department of Microbiology Second Military Medical University Shanghai 200433 China.

The spike (S) protein, a main surface antigen of SARS-coronavirus (SARS-CoV), is one of themost important antigen candidates for vaccine design. In the present study, three fragments of the truncated S protein were expressed in E. coli, and analyzed with pooled sera of convalescence phase of SARS patients. The full length S gene DNA vaccine was constructed and used to immunize BALB/c mice. The mouse serum IgG antibody against SARS-CoV was measured by ELISA with E. coli expressed truncated S protein or SARS-CoV lysate as diagnostic antigen. The results showed that all the three fragments of S protein expressed by E. coli was able to react with sera of SARS patients and the S gene DNA candidate vaccine could induce the production of specific IgG antibody against SARS-CoV efficiently in mice with seroconversion ratio of 75% after 3 times of immunization. These findings lay some foundations for further understanding the immunology of SARS-CoV and developing SARS vaccines.

关键词： severe acute respiratory syndrome-coronavirus (SARS-CoV) spike protein DNA vaccine antibody

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

试论写作活动中的意象思维

引用

广西师范大学学报（哲学社会科学版） 2009年第3期45卷 79-83页

作者：覃可霖广西师范学院文学院广西南宁530001

意象是用来寄托主观情思的客观物象。意象一般由客观之物和主观之意两部分组成,是两者的完美融合。意象思维是为"尽意"而"立象"的思维活动过程。意象思维在写作活动中的作用可分为三种:一是言不尽意,立象以尽意;二是形成情感的对应类象... 详细信息

意象是用来寄托主观情思的客观物象。意象一般由客观之物和主观之意两部分组成,是两者的完美融合。意象思维是为"尽意"而"立象"的思维活动过程。意象思维在写作活动中的作用可分为三种:一是言不尽意,立象以尽意;二是形成情感的对应类象;三是促进作品的自为性发展,彰显作者个性。意象思维具有多相意蕴,在写作活动中发挥着不可忽视的作用。

关键词：写作活动意象意象思维立象尽意多相意蕴

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

试论写作活动中的具象思维

引用

广西师范大学学报（哲学社会科学版） 2012年第1期48卷 113-116页

作者：覃可霖广西师范学院文学院广西南宁530007

写作是人类进入文明历史的一项主要标志。写作活动其实就是人们运用语言符号制作文章的一种精神劳动,即写作思维活动。具象思维是写作活动的一种基本思维方式,是写作活动的基础,是生动、具体的可感的审美形象思维。具象性、创造性、客... 详细信息

写作是人类进入文明历史的一项主要标志。写作活动其实就是人们运用语言符号制作文章的一种精神劳动,即写作思维活动。具象思维是写作活动的一种基本思维方式,是写作活动的基础,是生动、具体的可感的审美形象思维。具象性、创造性、客观性、整体性是其特征。通过激发兴趣积累意象、发展想象力可以培养和提高具象思维的能力进而提高写作水平。

关键词：写作活动具象思维具象思维特征

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

CAG-repeat variant in the polymerase γ gene and male infertility in the Chinese population： a meta-analysis

引用

Asian Journal of Andrology 2011年第2期13卷 298-304页

作者： Shu-Yuan Liu Chang-Jun Zhang Hai-Ying Peng Yu-Feng Yao Lei Shi Jin-Bao Chen ke-qin lin Liang Yu Li Shi Xiao-qin Huang Hao Sun Jia-You Chu Department of Medical Genetics Institute of Medical Biology Chinese Academy of Medical Sciences and Peking Union Medical College Kunming 650118 China Reproductive of Medical Research Centre of People＇s Hospital of Shiyan 442000 Shiyan China

Several studies have reported a relationship between the length of the CAG-repeat in the polymerase y （POLG） gene and male infertility. However, other studies have not reproduced this result. In our study, the POLG-CAG-repeat length was analyzed in 535 healthy individuals from six Chinese Han populations living in different provinces. The frequencies of IO-CAG alleles and genotypes were high （97.38 and 94.13%, respectively）, with no significant difference among the six Chinese Han populations. Furthermore, we determined the distribution of the POLG-CAG-repeat in 150 infertile men and 126 fertile men. Our study suggested that the distributions of POLG-CAG-repeat alleles and genotypes were not significantly different between infertile （95.67 and 92.67%, respectively） and fertile men （97.22 and 94.44%, respectively）. In a subsequent meta-analysis, combining our data with data from previous studies, a comparison of the CAG-repeat alleles in fertile versus infertile men showed no obvious risk for male infertility associated with any particular allele （pooled odds ratio （0R）=0.94; 95% confidence interval （CI）： 0.60-1.48）. The significance level was not attained with any of the following genetic models： homozygote comparison （not lO/not 10 versus 10110： OR= 1.34; 95% Ch 0.66-2.72）, heterozygote comparison （lO/not 10 versus 10/10： OR= 1.04; 95% Ch 0.78-1.38）, dominant model comparison （not lO/not 10＋ 101 not 10 versus 10110. OR= 1.08; 95% Ch 0.79-1.47） and recessive genetic comparison （not lO/not 10 versus lO/not 10＋ 10/10- OR= 1.31; 95% Ch 0.68-2.55）. In conclusion, there is no significant difference of the frequencies of POLG-CAG-repeat variants among six Chinese Han populations, and this polymorphism may not be associated with Chinese male infertility. On the basis of a meta-analysis, there is no obvious association between CAG-repeat variants of the POLG gene and male infertility.

关键词： case-control study male infertility meta-analysis POLG-CAG-repeat

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Electrocatalytic cleavage of a lignin β-O-4 model compound and coupling with nitrogen-containing aromatics using Prussian blue analogue-derived nickel–cobalt spinel

引用

Journal of Energy Chemistry 2025年第1期100卷 628-636页

作者： Yi Qi Xueying Chen Bowen Liu Xuliang lin Xueqing Qiu Yanlin qin Guangdong Provincial key Laboratory of Plant Resources Biorefinery School of Chemical Engineering and Light Industry Guangdong University of Technology Guangdong Provincial Laboratory of Chemistry and Fine Chemical Engineering Jieyang Center Guangdong Basic Research Center of Excellence for Ecological Security and Green Development Guangdong University of Technology

Electrochemical conversion of lignin for the production of high-value heterocyclic aromatic compounds has great *** demonstrate the targeted synthesis and cation modulation of NiCo2O4spinel nanoboxes,synthesized via cation exchange and calcination *** catalysts exhibit excellent efficacy in the electrocatalytic conversion of lignin model compounds,specifically 2-phenoxy-1-phenylethanol,into nitrogen-containing aromatics,achieving high conversion rates and *** catalysts were synthesized via a cation exchange and calcination oxidation process,using Prussian blue nanocubes as *** porous architecture and polymetallic composition of the NiCo2O4spinel demonstrated superior performance in electrocatalytic oxidative coupling,achieving a 99.2 wt%conversion rate of the 2-phenoxy-1-phenylethanol with selectivities of 37.5 wt%for quinoline derivatives and 31.5 wt%for *** innovations include the development of a sustainable one-pot synthesis method for quinoline derivatives,the elucidation of a multistage reaction pathway involving CAO bond cleavage,hydroxyaldol condensation,and CAN bond formation,and a deeper mechanistic understanding derived from DFT *** work establishes a new strategy for lignin valorization,offering a sustainable route to produce high-value nitrogen-containing aromatics from renewable biomass under mild conditions,without the need for additional reagents.

关键词： Spinel Biomass valorization Electrocatalysis Lignin oxidation Coupling

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

论写作活动中的相似思维——写作思维理论探索之二

引用

广西师范学院学报（哲学社会科学版） 2007年第2期28卷 139-144页

作者：覃可霖广西师范学院中文学院广西南宁530001

在写作活动中,相似现象大量存在于客观事物和认识主体思维活动中,写作过程其实也就是人脑对复杂信息的处理过程,输入的复杂信息,可以激活大脑中原已储存的相似块即相似信息。所以我们既要研究静态相似情况,更要注重动态过程的研究。运... 详细信息

在写作活动中,相似现象大量存在于客观事物和认识主体思维活动中,写作过程其实也就是人脑对复杂信息的处理过程,输入的复杂信息,可以激活大脑中原已储存的相似块即相似信息。所以我们既要研究静态相似情况,更要注重动态过程的研究。运用相似思维进行构思、模仿,并在语言运动方面,把事物的相似与模仿结合起来,在写作中加以运用,以切实、有效提高写作水平。

关键词：相似思维相似块相似模仿构思

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

再论写作思维与写作技巧的关系

引用

广西师范学院学报（哲学社会科学版） 2009年第2期30卷 120-124,152页

作者：覃可霖广西师范学院文学院广西南宁530001

写作是一门思维的艺术,同时也是一门离不开写作技巧的活动。写作思维对写作技巧影响的例子俯拾皆是,如通感思维、模糊思维、反常思维等。在写作活动中既要对写作主体进行写作思维的训练,也要进行写作技巧的培养,才能切实提高写作主体的... 详细信息

写作是一门思维的艺术,同时也是一门离不开写作技巧的活动。写作思维对写作技巧影响的例子俯拾皆是,如通感思维、模糊思维、反常思维等。在写作活动中既要对写作主体进行写作思维的训练,也要进行写作技巧的培养,才能切实提高写作主体的写作能力。

关键词：写作思维通感思维模糊思维反常思维思维能力

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：