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Enhanced Resistance of Snowdrop Lectin （Galanthus nivalis L. agglutinin） Expressing Maize to Asian Corn Borer （Ostriniafurnacalis Guenée）

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Journal of Integrative Plant Biology 2005年第7期47卷 873-880页

作者： Zhao-YuWANG Xiao-FenSUN FeiWANG ke-xuantang Ju-RenZHANG SchoolofLifeSciences ShandongUniversityJinan250100China StatekeyLaboratoryofGeneticEngineering InstituteofGeneticsSchoolofLifeSciencesFudanUniversityShanghai200433China SchoolofAgricultureandBiology ShanghaiJiaoTongUniversityShanghai200030China

In order to enhance the resistance to pests, transgenic maize (Zea mays L.) plants from elite inbred lines containing the gene encoding snowdrop lectin (Galanthus nivalis L. agglutinin; GNA) under control of a phloem-specific promoter were generated through the Agrobacterium tumefaciens-mediated also studied. Thirty-six independently derived plants were subjected to molecular analyses. The level of GNA expression at 0.13%-0.28% of total soluble protein was observed in different transgenic plants. The progeny of three GNA-expressing independent transformants that were derived separately from the elite inbred lines DH4866, DH9942, and 8902, were selected for examination of resistance to ACB. These plants synthesized GNA at levels above 0.24% total soluble protein and enhanced resistance to ACB was demonstrated by exposing the plants to insects under greenhouse conditions. Semi-artificial diet bioassays also showed the toxic effect of GNA on ACB. Field evaluation of the transgenic plants supported the results from the artificial trial. In the present study, we have obtained new insect-resistant maize material for further breeding work and have found that GNA-expressing plants not only gained significant resistance to homopterans, but also showed toxicity to ACB, which is a type of Lepidoptera.

关键词： com borer Galanthus nivalis agglutinin gene(gna) maize resistance to pests transgene.

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Metabolic Engineering of Tropane Alkaloid Biosynthesis in Plants

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Journal of Integrative Plant Biology 2005年第2期47卷 136-143页

作者： LeiZHANG Guo-YinKAI Bei-BeiLU Han-MingZHANG ke-xuantang Ji-HongJIANG Wan-ShengCHEN[ SchoolofPharmacy TheSecondMilitaryMedicalUniversityShanghai200433China／／KeyLaboratoryforBiotechnologyonMedicinalPlantofJiangsuProvinceXuzhouNormalUniversityXuzhou221116China PlantBiotechnologyResearchCenter SchoolofAgricultureandBiologyFudan-ShanghaiJiaotongUniversity-NottinghamPlantBiotechnologyResearchandDevelopmentCenterSchoolofLifeSciencesandTechnologyShanghaiJiaotongUniversity SchoolofPharmacy TheSecondMilitaryMedicalUniversityShanghai200433China keyLaboratoryforBiotechnologyonMedicinalPlantofJiangsuProvince XuzhouNormalUniversityXuzhou221116China

Abstract: Over the past decade, the evolving commercial importance of so-called plant secondary metabolites has resulted in a great interest in secondary metabolism and, particularly, in the possibilities to enhance the yield of fine metabolites by means of genetic engineering. Plant alkaloids, which constitute one of the largest groups of natural products, provide many pharmacologically active compounds. Several genes in the tropane alkaloids biosynthesis pathways have been cloned, making the metabolic engineering of these alkaloids possible. The content of the target chemical scopolamine could be significantly increased by various approaches, such as introducing genes encoding the key biosynthetic enzymes or genes encoding regulatory proteins to overcome the specific rate-limiting steps. In addition, antisense genes have been used to block competitive pathways. These investigations have opened up new, promising perspectives for increased production in plants or plant cell culture. Recent achievements have been made in the metabolic engineering of plant tropane alkaloids and some new powerful strategies are reviewed in the present paper.

关键词： biosynthesis pathway genetic transformation hyoscyamine plant secondary metabolic engineering scopolamine tropane alkaloids

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钱玄同对康有为经学思想的承继与超越

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北方论丛 2008年第2期 99-102页

作者：李可亭郑州大学历史系河南郑州450001

钱玄同一方面受康有为经学思想很大的影响,对《新学伪经考》也多有誉美之辞,而另一方面二人所走的并不是一条同样的道路。可以说,康有为是近代的今文经学家,近代今文经学的集大成者;而钱玄同则不泥家派,超越今古又超越经学,是埋葬经学... 详细信息

钱玄同一方面受康有为经学思想很大的影响,对《新学伪经考》也多有誉美之辞,而另一方面二人所走的并不是一条同样的道路。可以说,康有为是近代的今文经学家,近代今文经学的集大成者;而钱玄同则不泥家派,超越今古又超越经学,是埋葬经学、变经学为史学的一个功臣。分析认识钱玄同对康有为经学思想的承继与超越,对于认识康有为和钱玄同的经学思想,以及中国近代经学的发展走向,有着重要的意义。

关键词：钱玄同康有为经学思想承继超越

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