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P(VPBA-DMAEA) as a pH-sensitive nanovalve for mesoporous silica nanoparticles based controlled release

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Chinese Chemical Letters 2015年第10期26卷 1203-1208页

作者： Yu-Jie Chang Xi-Zhen Liu Qing Zhao Xiao-Hai Yang ke-min wang Qing wang min Lin Meng Yang State key Laboratory of Chemo/Biosensing and Chemometrics College of Chemistry and Chemical Engineering Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province Hunan University

A pH-sensitive controlled release system was proposed in this work, which consists of mesoporous silica nanoparticles（MSNs） functionalized on the pore outlets with poly（4-vinylphenybronic acid-co-2-（dimethylamino）ethyl acrylate） [P（VPBA-DMAEA）]. Four kinds of P（VPBA-DMAEA）-gated MSNs were synthesized and applied for the p H-sensitive controlled release. The results showed that P（VPBADMAEA） can work as a p H-sensitive nanovalve. The release behavior of the hybrid nanoparticles could be adjusted by changing the mole ratio of VPBA and DMAEA. With the increasing of the mole ratio of VPBA,the leakage of the entrapped molecules in the pores of MSNs could be decreased at neutral and alkaline conditions. By altering the p H of buffer from 4.0 to 8.0, the valve could be switched ‘‘on＇＇ and ‘‘off＇＇reversibly. In addition, cells viability results indicated that these P（VPBA-DMAEA）-gated MSNs had good biocompatibility. We believe that these MSNs based p H-sensitive controlled release system will provide a promising nanodevice for sited release of drug delivery.

关键词： pH-sensitive Mesoporous silica nanoparticles Nanovalve Polymer

A facile route for shape-selective synthesis of silica nanostructures using poly-L-lysine as template

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Chinese Chemical Letters 2013年第2期24卷 99-102页

作者： Ding-Geng He Xiao-Xiao He ke-min wang Ying-Xiang Zhao State key Laboratory of Chemo/Biosensing and Chemometrics College of Chemistry and Chemical EngineeringHunan University key Laboratory for Bio-Nanotechnotogy and Molecule Engineering of Hunan Province College of BiologyHunan University key Laboratory for Bio-Nanotechnology and Molecule Engineering of Hunan Province College of Biology.Hunan University key Laboratory for Bio-Nanotechnology and Molecule Engineering of Hunan Province College of BiologyHunan University

Received 12 December 2012 Received in revised form 7 January 2013 Accepted 9 January 2013 Available online 8 February 2013

关键词： Poly-l-lysineReverse microemulsionSilica nanostructureTEOS

Real-time monitoring of DNAzyme cleavage process using fluorescent assay

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Chinese Chemical Letters 2009年第8期20卷 990-994页

作者： Xiang Xian Meng Xiao Hai Yang ke min wang Wei Hong Tan Qiu Ping Guo State key Laboratory of Chemo/Biosensing and Chemometrics Engineering Center for BiomedicineKey Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan ProvinceCollege of Chemistry and Chemical Engineering Hunan University Changsha 410082 China

Detection of deoxyribozyme （DNAzyme） cleavage process usually needs complex and time-consuming radial labeling, gel electrophoresis and autoradiography. This paper reported an approach to detect DNAzyme cleavage process in real time using a fluorescence probe. The probe was employed as DNAzyme substrate to convert directly the cleavage information into fluorescence signal in real time. Compared with traditional approach, this non-isotope method not only brought a convenient means to monitor the DNAzyme cleavage reaction, but also offered abundant dynamic data for choosing potential gene therapeutic agents. It provides a new tool for DNAzyme research, as well as a new insight into research on human disease diagnosis. Based on this method, 8- 17deoxyribozyme （8-17DNAzyme） against hepatitis C virus RNA （HCV-RNA） was designed and the cleavage process was studied in real time. ？2009 ke min wang. Published by Elsevier B.V. on behalf of Chinese Chemical Society. All fights reserved.

关键词： DNAzyme Cleavage Real time HCV-RNA

A novel fluorescent detection for PDGF-BB based on dsDNA-templated copper nanoparticles

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Chinese Chemical Letters 2014年第1期25卷 9-14页

作者： Xiao-Hai Yang Shan Sun Pei liu ke-min wang Qing wang Jian-Bo Liu Jin Huang Lei-Liang He State key Laboratory of Chemo/Biosensing and Chemometrics College of Chemistry and Chemical EngineeringHunan UniversityKey Laboratory for Bio-nanotechnology and Molecular Engineering of Hunan Province

A novel method for the detection of PDGF-BB has been developed using double-strand DNA-copper nanoparticles （dsDNA-CuNPs） as fluorescent markers. This assay relies on the premise that the aptamer- based probe undergoes a conformational change upon binding with target protein, and subsequently triggers polymerization reaction to generate dsDNA. Then, the resultant dsDNA can be used as a template for the formation of CuNPs with high fluorescence. Under the optimized conditions, the proposed assay allowed sensitive and selective detection of PDGF-BB with a detection limit of 4 nmol/L. This possibly makes it an attractive platform for the detection of a variety of biomolecules whose aptamers undergo similar conformational change.

关键词： Copper nanoparticles PDGF-BB Polymerization

Sensitive detection of DNA methyltransferase activity based on rolling circle amplification technology

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Chinese Chemical Letters 2014年第7期25卷 1047-1051页

作者： Pei Liu Xiao-Hai Yang Qing wang Jing Huang Jian-Bo Liu Ying Zhu Lei-Liang He ke-min wang State key Laboratory of Chemo/Biosensing and Chemometrics College of Chemistry and Chemical EngineeringKey Laboratory for Bio-nanotechnology and Molecular Engineering of Hunan ProvinceHunan University

This work develops a fluorescence approach for sensitive detection of DNA methyltransferase activity based on endonuclease and rolling circle amplification （RCA） technique. In the presence of DNA adenine methylation （Dam） MTase, the methylation-responsive sequence of hairpin probe is methylated and cleaved by the methylation-sensitive restriction endonuclease Dpn 1. The products cleaved by restriction endonuclease Dpn I then function as a signal primer to initiate RCA reaction by hybridizing with the circular DNA template. Each RCA product containing thousands of repeated sequences might hybridize with a large number of molecular beacons （detection probes）, resulting in an enhanced fluorescence signal. In the absence of Dam MTase, neither methylation/cleavage nor RCA reaction can be initiated and no fluorescence signal is observed. The proposed method exhibits a dynamic range from 0.5 U/mL to 30 U/mL and a detection limit of 0.18 U/mL. This method can be used for the screening of antimicrobial drugs and has a great potential to be further applied in early clinical diagnosis.

关键词： Methyltransferase RCAD am MTase Dpn 1 Fluorescence

A facile approach toward multicolor polymers:Supramolecular self-assembly via host-guest interaction

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Chinese Chemical Letters 2014年第10期25卷 1318-1322页

作者： Xiao-Hai Yang Fang Zhao Lei-Liang He ke-min wang Jin Huang Qing wang Jian-Bo Liu Meng Yang State key Laboratory of Chemo/Biosensing and Chemometrics College of Chemistry and Chemical EngineeringKey Laboratory for Bio-nanotechnology and Molecular Engineering of Hunan ProvinceHunan University

A one-step and facile strategy toward the construction of multicolor polymers via supramolecular selfassembly was *** polymers were simply prepared by the self-assembly of adamantane-labeled fluorescein,adamantane-labeled rhodamine B and β-cyclodextrin polymers via host-guest interaction between β-cyclodextrin and *** polymers showed several interesting properties：multiple emission signatures by a single wavelength excitation,easy tunability,intense fluorescence,high *** addition,the self-assembly approach implied a facile and flexible strategy for constructing functionalized materials,such as multicolor materials for biological labeling and imaging,and sensing materials for the detection of physiological parameters.

关键词： Multicolor β-Cyclodextrin polymer Self-assembly Host-guest interaction

One pot synthesis of Ru(bpy)_3^(2+) doped graphene oxide-silica composite film for constructing high performance solid-state electrochemiluminescent sensor

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Chinese Chemical Letters 2014年第12期25卷 1520-1524页

作者： Gen-Ping Yan Xiao-Xiao He ke-min wang Yong-Hong wang Jin-Quan Liu Li-Xin Jian Yin-Fei Mao State key Laboratory of Chemo/Biosensing and Chemometrics College of Chemistry and Chemical EngineeringCollege of BiologyKey Laboratory for Bio-nanotechnology and Molecular Engineering of Hunan ProvinceHunan University

The Ru（bpy）3^2＋ doped graphene oxide-silica composite film（Ru/GO-SiCF） was synthesized by one pot hydrolysis and condensation of tetraethylorthosilicate（TEOS） in the water-alcohol solution of graphene oxide and Ru（bpy）3^2＋ at room *** prepared Ru/GO-SiCF modified glassy carbon electrode（GCE） showed excellent electrochemiluminescence（ECL） behavior for the determination of tripropylamine（TPA） with high sensitivity and good *** expected this simple and novel material will find further application in construction of other targets sensors.

关键词： Graphene oxide(GO)-silica composite Electrochemiluminescence(ECL) Ru(bpy)3^2+ Sensor

DNA aptamer selected for specific recognition of prostate cancer cells and clinical tissues

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Chinese Chemical Letters 2017年第6期28卷 1252-1257页

作者： Zhi-Xiang Huang Qin Xie Qiu-Ping Guo ke-min wang Xiang-Xian Meng Bao-Yin Yuan Jun Wan Yuan-Yuan Chen State key Laboratory of Chemo/Biosensing and Chemometrics College of Biology College of Chemistry and Chemical Engineering Key Laboratory for Bio-Nanotechnology and Molecular Engineering of Hunan Province Hunan University Changsha 410082 China

Prostate cancer is the most common malignancy in men lack of efficient early diagnosis and therapeutics,calling for effective molecular ***,we performed cell-based systematic evolution of ligands by exponential enrichment（cell-SELEX） to obtain specific recognition of human prostate cancer cells *** aptamers were successfully obtained that can bind to target cells with high affinity and specificity.A 51-nt truncated sequence named Xq-2-C1 was identified after further elaborative analysis on the secondary *** importantly,the achieved aptamer Xq-2-C1 not only demonstrated excellent specific to target cells,but also revealed specific recognition to clinical prostate cancer *** tissue imaging results showed that Xq-2-C1 had better recognition ratio for clinical prostate cancer tissue samples（85%） compared to the random sequence（9%）.These results demonstrate that these newly generated aptamers would furnish potential applications in the early diagnosis and clinical treatment of prostate cancer.

关键词： Aptamer Cell-SELEX Prostate cancer PC-3M Clinical tissues

STATICALLY THERMOMAGNETIC WRITING AND MICRON-DOMAIN OBSERVATION IN TbFeCo MAGNETO-OPTICAL DISC

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Chinese Science Bulletin 1989年第15期34卷 1305-1309页

作者：杨克敏王焕元王荫君 Institute of Physics Academia SinicaBeijing 100080

Amorphous rare earth-transition metal (RE-TM) alloy films with perpendicular anisotropy have been considered as more suitable materials for high recording density in the erasable magneto-optical disc. In recent years, there are considerable reports describing the magneto-optical read/write characteristics on such amorphous films, e.g. TbFeCo and GdTbFe.

关键词： disc micron-domain thermomagnetic-writing light-modulation magneticfield-modulation