检索结果-南通市图书馆

Impaired suppressive activities of human MUTYH variant proteins against oxidative mutagenesis

World Journal of Gastroenterology 2012年第47期18卷 6935-6942页

作者： kazuya shinmura Masanori Goto Hong Tao Shun Matsuura Tomonari Matsuda Haruhiko Sugimura Department of Tumor Pathology Hamamatsu University School of MedicineShizuoka 431-3192Japan Research Center for Environmental Quality Management Kyoto UniversityShiga 520-0811Japan

AIM:To investigate the suppressive activity of MUTYH variant proteins against mutations caused by oxidative lesion,8-hydroxyguanine(8OHG),in human ***:p.R154H,p.M255V,p.L360P,and p.P377L MUTYH variants,which were previously found in patients with colorectal polyposis and cancer,were selected for use in this *** H1299 cancer cell lines inducibly expressing wild-type(WT) MUTYH(type 2) or one of the 4 above-mentioned MUTYH variants were established using the piggyBac transposon vector system,enabling the genomic integration of the transposon sequence for MUTYH *** expression was examined after cumate induction using Western blotting analysis and immunofluorescence *** intracellular localization of MUTYH variants tagged with FLAG was also immunofluorescently ***,the mutation frequency in the supF of the shuttle plasmid pMY189 containing a single 8OHG residue at position 159 of the supF was compared between empty vector cells and cells expressing WT MUTYH or one of the 4 MUTYH variants using a supF forward mutation ***:The successful establishment of human cell lines inducibly expressing WT MUTYH or one of the 4 MUTYH variants was concluded based on the detection of MUTYH expression in these cell lines after treatment with *** of the MUTYH variants and WT MUTYH were localized in the nucleus,and nuclear localization was also observed for FLAG-tagged *** mutation frequency of supF was 2.2 × 10-2 in the 8OHG-containing pMY189 plasmid and 2.5 × 10-4 in WT pMY189 in empty vector cells,which was an 86-fold increase with the introduction of *** mutation frequency(4.7 × 10-3) of supF in the 8OHG-containing pMY189 plasmid in cells overexpressing WT MUTYH was significantly lower than in the empty vector cells(P < 0.01).However,the mutation frequencies of the supF in the 8OHG-containing pMY189 plasmid in cells overexpressing the p.R154H,p.M255V,p.L360P,or p.P377L MUTYH variant were 1.84 × 10-2,1.55 × 10-2

关键词： 8-hydroxyguanine Mutation MUTYH MUTYH-associated polyposis Oxidative mutagenesis supF forward mutation assay piggyBac transposon Colorectal polyposis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Tuberous sclerosis patient with neuroendocrine carcinoma of the esophagogastric junction:A case report

引用

World Journal of Gastroenterology 2020年第45期26卷 7263-7271页

作者： Natsuki Ishida Takahiro Miyazu Satoshi Tamura Satoshi Suzuki Shinya Tani Mihoko Yamade Moriya Iwaizumi Satoshi Osawa Yasushi Hamaya kazuya shinmura Haruhiko Sugimura Katsutoshi Miura Takahisa Furuta Ken Sugimoto First Department of Medicine Hamamatsu University School of MedicineHamamatsu 431-3192Japan Department of Endoscopic and Photodynamic Medicine Hamamatsu University School of MedicineHamamatsu 431-3192Japan Department of Laboratory Medicine Hamamatsu University School of MedicineHamamatsu 431-3192Japan Haruhiko Sugimura Department of Tumor PathologyHamamatsu University School of MedicineHamamatsu 431-3192Japan Department of Health Science Hamamatsu University School of MedicineHamamatsu 431-3192Japan Center for Clinical Research Hamamatsu University School of MedicineHamamatsu 431-3192Japan

BACKGROUND Tuberous sclerosis complex(TSC)is a rare inherited disease with non-cancerous tumor growths in the skin,brain,kidneys,heart,and *** co-occurrence of neuroendocrine neoplasm(NEN)with TSC is even *** have been few reports on the relationship between TSC and neuroendocrine tumors(NETs),and fewer on the relationship between TSC and neuroendocrine carcinoma(NEC),a subtype of *** is the first reported case of NEC occurring at the esophagogastric junction in a patient with *** SUMMARY A 46-year-old woman visiting our hospital for the treatment of TSC was admitted to the emergency department with tarry stools and *** tomography scans revealed thickness of the gastric cardia,multiple metastatic lesions of the liver,and enlarged lymph nodes near the lesser curvature of the *** revealed a type 3 tumor located from the esophagogastric junction to the fundus,and the pathological diagnosis by biopsy was *** patient was treated with seven courses of cisplatin+irinotecan,followed by eight courses of ramucirumab+nab-paclitaxel,one course of nivolumab,and two courses of S-1+***-three months after the first treatment,the patient died because of disease progression and deterioration of the general *** This case of NEC occurring in a patient with TSC indicates a difference in the occurrence of NETs and NECs.

关键词： Tuberous sclerosis complex Neuroendocrine carcinoma Neuroendocrine tumor mTOR inhibitor Esophagogastric junction Chemotherapy Case report

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Three novel NEIL1 promoter polymorphisms in gastric cancer patients

引用

World Journal of Gastrointestinal Oncology 2010年第2期2卷 117-120页

作者： Masanori Goto kazuya shinmura Shoichiro Tsugane Haruhiko Sugimura First Department of Pathology Hamamatsu University School of Medicine1-20-1 HandayamaHigashi WardHamamatsuShizuoka 431-3192Japan Epidemiology and Prevention Division Research Center for Cancer Prevention and ScreeningNational Cancer CenterTokyo 104-0045Japan

AIM:To identify genetic polymorphisms in the promoter region of the human base excision repair gene NEIL1 in gastric cancer ***:The NEIL1 promoter region in DNA from 80 Japanese patients with gastric cancer was searched for genetic polymorphisms by polymerase chain reaction-single-strand conformation polymorphism and subsequent sequencing ***:Three novel genetic polymorphisms,i.e.c.-3769C>T,c.-3170T>G,and c.-2681TA[8],were identified in the NEIL1 promoter region at an allele frequency of 0.6%,9.4%,and 4.4%,respectively,in Japanese gastric cancer ***:Three NEIL1 promoter polymorphisms detected in this study may be of importance in gastric carcinogenesis.

关键词： Gastric cancer NEIL1 Base excision repair Genetic polymorphism

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

EPH-EPHRIN in human gastrointestinal cancers

引用

World Journal of Gastrointestinal Oncology 2010年第12期2卷 421-428页

作者： Haruhiko Sugimura Hiroki Mori Masaru Tsuboi Kiyoko Nagura Hisaki Igarashi Ritsuko Nakamura Hiroko Natsume Tomoaki Kahyo kazuya shinmura Hiroyuki Konno Yasushi Hamaya Shigeru Kanaoka Hideki Kataoka Department of Pathology I Hamamatsu University School of MedicineHamamatsu 431-3192Japan Department of Surgery II Hamamatsu Uni- versity School of MedicineHamamatsu431-3192Japan Department of Molecular Diagnostics Hamamatsu University School of MedicineHamamatsu 431-3192Japan Department of Medicine I Hamamatsu University School of MedicineHamamatsu431-3192Japan

Ever since its discovery two decades ago,the erythro- poietin-producing hepatoma (EPH)-EPHRIN system has been shown to play multifaceted roles in human gastroenterological cancer as well as ***,amplif ication and point mutations have been found in many human cancers and many investigators have shown correlations between these up-regulationsand tumor ***,the genes in this family are considered to be potential targets of cancer *** the other hand,the down-regulation of some members as a result of epigenetic changes has also been reported in some ***,the correlation between altered expressions and clinical prognosis seems to be inconclusive.A huge amount of protein-protein interaction studies on the EPH-EPHRIN system have provided a basic scheme for signal transductions,especially bi-directional signaling involving EPH-ERPHRIN molecules at the cell *** information also provides a manipulative strategy for harnessing the actions of these *** this review,we summarize the known alterations of EPH-EPHRIN genes in human tumors of the esophagus,stomach,colorectum,liver and pancreas and present the perspective that the EPH-EPHRIN system could be a potential target of cancer therapy.

关键词： Erythropoietin-producing hepatoma EPH-EPHRIN Gastric cancer Colorectal cancer Methylation Secreted form

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Discriminant Analysis of the Linear Separable Data - Japanese 44 Cars

引用

Journal of Statistical Science and Application 2016年第4期4卷 165-178页

作者： Shuichi shinmura Faculty of Economics Seikei University Japan

There are four serious problems in the discriminant analysis. We developed an optimal linear discriminant function （optimal LDF） based on the minimum number of misclassification （minimum NM） using integer programming （IP）. We call this LDF as Revised IP-OLDF. Only this LDF can discriminate the cases on the discriminant hyperplane （Probleml）. This LDF and a hard-margin SVM （H-SVM） can discriminate the lineary separable data （LSD） exactly. Another LDFs may not discriminate the LSD theoretically （Problem2）. When Revised IP-OLDF discriminate the Swiss banknote data with six variables, we find MNM of two-variables model such as （X4, X6） is zero. Because MNMk decreases monotounusly （MNMk 〉= MNM（k＋1））, sixteen MNMs including （X4, X6） are zero. Until now, because there is no research of the LSD, we surveyed another three linear separable data sets such as： 18 exam scores data sets, the Japanese 44 cars data and six microarray datasets. When we discriminate the exam scores with MNM=0, we find the generalized inverse matrix technique causes the serious Problem3 and confirmed this fact by the cars data. At last, we claim the discriminant analysis is not the inferential statistics because there is no standard errors （SEs） of error rates and discriminant coefficients （Problem4）. Therefore, we poroposed the ＂100-fold cross validation for the small sample＂ method （the method）. By this break-through, we can choose the best model having minimum mean of error rate （M2） in the validation sample and obtaine two 95% confidence intervals （CIs） of error rate and discriminant coefficients. When we discriminate the exam scores by this new method, we obtaine the surprising results seven LDFs except for Fisher＇s LDF are almost the same as the trivial LDFs. In this research, we discriminate the Japanese 44 cars data because we can discuss four problems. There are six independent variables to discriminate 29 regular cars and 15 small cars. This data is linear separable by the emission rate （X1）

关键词： Model Selection Procedure Means of Error Rates Fisher's LDF Logistic Regression Support VectorMachine (SVM) Minimum Number of Misclassifications (minimum NM MNM) Revised IP-OLDF based onMNM criterion Revised IPLP-OLDF Revised LP-OLDF Linear Separable Data and Model K-fold Crossvalidation.

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Hot snare polypectomy vs endoscopic mucosal resection using bipolar snare for intermediate size colorectal lesions:Propensity score matching

引用

World Journal of Gastroenterology 2023年第23期29卷 3668-3677页

作者： Nobuhisa Minakata Tatsuro Murano Masashi Wakabayashi Maasa Sasabe Takashi Watanabe Tomohiro Mitsui Hiroki Yamashita Atsushi Inaba Hironori Sunakawa Keiichiro Nakajo Tomohiro Kadota Kensuke shinmura Hiroaki Ikematsu Tomonori Yano Department of Gastroenterology and Endoscopy National Cancer Center Hospital EastKashiwa 2778577ChibaJapan Department of Biostatistics Division Center for Research Administration and SupportNational Cancer CenterKashiwa 2778577ChibaJapan

BACKGROUND Endoscopic resection(ER)with bipolar snare,in which the electric current only passes through the tissue between the device’s two electrodes,is a prominent method used to prevent perforation due to electricity *** using bipolar snare with or without submucosal injection enabled safe resection of colorectal lesions measuring 10-15 mm in an ex vivo porcine *** with bipolar snare is expected to have good treatment outcomes in 10-15 mm colorectal lesions,with high safety even without submucosal ***,no clinical reports have compared treatment outcomes with and without submucosal *** To compare the treatment outcomes of bipolar polypectomy with hot snare polypectomy(HSP)to those with endoscopic mucosal resection(EMR).METHODS In this single-centre retrospective study,we enrolled 10-15 mm nonpedunculated colorectal lesions(565 Lesions in 463 patients)diagnosed as type 2A based on the Japan Narrow-band Imaging Expert Team classification,resected by either HSP or EMR between January 2018 and June 2021 at the National Cancer Center Hospital *** were divided into HSP and EMR groups,and propensity score matching was *** the matched cohort,en bloc and R0 resection rates and adverse events were compared between the two *** Of the 565 lesions in 463 patients,117 lesions each in the HSP and EMR groups were selected after propensity score *** the original cohort,there was a significant difference in antithrombotic drug use(P<0.05),lesion size(P<0.01),location(P<0.01),and macroscopic type(P<0.05)between the HSP and EMR *** the matched cohort,the en bloc resection rates were comparable between both groups[93.2%(109/117)vs 92.3%(108/117),P=0.81],and there was no significant difference in the R0 resection rate[77.8%(91/117)vs 80.3%(94/117),P=0.64].The incidence of delayed bleeding was similar in both groups[1.7%(2/117)].Perforation occurred in the EMR group[0.9%(1/117)]but not in the HSP ***

关键词： Adenoma Cohort studies Colonoscopy Colorectal cancer Endoscopic mucosal resection Treatment outcome

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Metformin Modulates GLP-1- and GIP-Mediated Intracellular Signaling under Normoglycemic Conditions

引用

Open Journal of Endocrine and Metabolic Diseases 2013年第7期3卷 263-270页

作者： Kyoko shinmura Takaharu Negoro Shunichi Shimizu Giovanna Roncador Tsutomu Hirano Yasuko Nakano Department of Pathophysiology Showa University School of Pharmacy Tokyo Japan Department of Pharmacogenomics Showa University School of Pharmacy Tokyo Japan Division of Diabetes Metabolism and Endocrinology Department of Internal Medicine Showa University School of Medicine Tokyo Japan Monoclonal Antibodies Unit Biotechnology Program Centro Nacional de Investigaciones Oncologicas (CNIO) Madrid Spain

GLP-1 and GIP promote insulin secretion from pancreatic β-cells by inducing intracellular signals such as Ca2+ and cAMP. Metformin primarily acts by inhibiting glucogenesis in the liver and promoting glucose metabolism in the muscle. It is used as a concomitant drug with the incretin in the treatment of T2D. We focused on intracellular signals under various glucose concentrations and assessed the effects of metformin on incretin signaling in MIN6 β-cells. Metformin inhibited incretin-induced [Ca2+]i in the presence of 5.5 mM glucose but not 16.7 mM glucose. In accordance with low [Ca2+]i, insulin secretion from MIN6 cells declined, despite enhanced incretin-induced cAMP production. Abundant expressions of Adcy 6 and 9, which are negatively controlled by Ca2+ signals, were detected in MIN6 cells. Thus, increasing cAMP production was associated with the inhibition of Ca2+ mobilization by metformin. However, we show that metformin controls insulin secretion by inhibiting incretin-mediated [Ca2+]i under normoglycemic conditions.

关键词： Metformin GLP-1 GIP Calcium cAMP Insulin Secretion

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

椎板成形术联合椎弓根钉固定治疗脊髓型颈椎病伴痉挛性脑瘫:10年以上随访研究

引用

中国骨科临床与基础研究杂志 2021年第1期13卷 61-61页

作者： Demura S Kato S shinmura K 白朝晖(摘译) 不详

脊髓型颈椎病伴痉挛性脑瘫患者存在颈椎畸形及颈部重复性不自主运动,易导致围手术期内固定失败,其手术治疗对于术者而言是一个挑战。本研究对采用椎板成形术联合椎弓根钉后路关节融合术患者进行至少10年的长期随访,旨在评估该术式的远... 详细信息

脊髓型颈椎病伴痉挛性脑瘫患者存在颈椎畸形及颈部重复性不自主运动,易导致围手术期内固定失败,其手术治疗对于术者而言是一个挑战。本研究对采用椎板成形术联合椎弓根钉后路关节融合术患者进行至少10年的长期随访,旨在评估该术式的远期疗效。

关键词：脊髓型颈椎病不自主运动关节融合术痉挛性脑瘫随访研究椎板成形术椎弓根钉长期随访

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

摄取转运蛋白OATP8在肝癌发生不同过程中表达减少:与钆塞酸增强MRI的相关性

引用

国际医学放射学杂志 2011年第6期 606-606页

作者： A. Kitao O. Matsui N. Yoneda K. Kozaka R. shinmura W. Koda 闫晴晴 Department of Radiology Kanazawa University Graduate School of Medical Science13-1 Takaramachi Kanazawa 920-8640 Japan

目的研究肝结节有机阴离子转运多肽8(OATP8)的表达和钆塞酸增强MRI的增强率在肝癌发生的不同过程中的变化。材料与方法在图像分析方面,对71例手术切除的肝细胞癌(高、中及低分化肝癌)和1个发育不良结节(DN),计算肝胆期钆塞酸增强MRI增强... 详细信息

目的研究肝结节有机阴离子转运多肽8(OATP8)的表达和钆塞酸增强MRI的增强率在肝癌发生的不同过程中的变化。材料与方法在图像分析方面,对71例手术切除的肝细胞癌(高、中及低分化肝癌)和1个发育不良结节(DN),计算肝胆期钆塞酸增强MRI增强率[(1/增强后T1值-

关键词：肝细胞癌肝癌发生 MRI 钆塞酸有机阴离子转运多肽8

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

肝细胞癌交界性病变的恶性富血供病灶的检测:动态MDCT、动态MRI和SPIO增强MRI的对照研究

引用

国际医学放射学杂志 2008年第A6期31卷 514-514页

作者： R.shinmura O.Matsui M.Kadoya S.Kobayashi N.Terayama J.Sana-da 林笑丰 Department of Radiology Toyama Prefectural Central Hospital 2-2-78 Nishinagae Toyama 930-8550 Japan.

本研究目的是回顾性分析肝动脉成像CT(CT dur-ing hepatic arteriography,CTHA)所发现硬化肝内交界性结节中的富血供病灶,比较动态MDCT。

关键词：肝恶性病灶肝细胞癌 CT MRI

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：