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Persistence of gene expression changes in noninflamed and inflamed colonic mucosa in ulcerative colitis and their presence in colonic carcinoma

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World Journal of Gastroenterology 2005年第33期11卷 5151-5155页

作者： Yuji Toiyama Akira Mizoguchi kazushi kimura Toshimitsu Araki Shigeyuki Yoshiyama Kouhei Sakaguchi Chikao Miki Masato Kusunoki The Second Department of Surgery Mie University School of Medicine 2-174 EdobashiTsuMie 514-8507Japan the Department of Anatomy Faculty of Medicine Mie UniversityEdobashiTsu Mie 514-8507 Japan The Second Department of Surgery Mie University School of Medicine 2-174 Edobashi Tsu Mie 514-8507Japan the Department of Anatomy Faculty of Medicine Mie University Edobashi Tsu Mie 514-8507 Japan The Second Department of Surgery Mie University School of Medicine 2-174 Edobashi Tsu Mie 514-8507 Japan

AIM： A few studies have applied genomic-wide gene expression analysis in inflamed colon tissue sample in ulcerative colitis （UC）. We reported the first study of non-inflamed mucosal gene expression in UC and explored its clinical implication. METHODS： Non-inflamed mucosa was obtained from 6 UC patients who received total colectomy. The gene expression of UC in noninflamed mucosa was monitored with a microarray. For a selected gene, RT-PCR was performed to verify array results and to further examine expression pattern in inflamed mucosa of UC, colorectal cancer tissue and normal mucosa using additional matched pairs. RESULTS： Two genes showing 2.5-fold decreased expression with significance set at in UC samples were borneo box a4 （HOXa4） and mads box transcription enhancer factor 2, polypeptide B （MEF2b）. RT-PCR showed that MEF2b expression in non-inflamed mucosa was significantly downregulated, whereas the expression of MEF2b increased in accordance with the severity of mucosal inflammation. HOXa4 expression both in inflamed and non-inflamed mucosa in UC was consistently downregulated, and the downregulation of HOXa4 was also found in colorectal carcinoma. CONCLUSION： It is suggested that the MEF2b expression in UC which increase in accordance with inflammation may be induced by the inflammatory mediator. In contrast the downregulation of HOXa4 may be partly involved in the pathogenesis of disease including UC and UC-related carcinogenesis.

关键词： Ulcerative colitis OligonucleoUde array Geneexpression

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TTYH2,a human homologue of the Drosophila melanogaster gene tweety,is up-regulated in colon carcinoma and involved in cell proliferation and cell aggregation

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World Journal of Gastroenterology 2007年第19期13卷 2717-2721页

作者： Yuji Toiyama Akira Mizoguchi kazushi kimura Junichirou Hiro Yasuhiro Inoue Tomonari Tutumi Chikao Miki Masato Kusunoki Department of Gastrointestinal and Pediatric Surgery Division of Reparative Medicine Institute of Life Sciences Mie University Graduate School of Medicine Japan Department of Anatomy Faculty of Medicine Mie University 2-174 EdobashiTsu Mie 514-8507 Japan

AIM: To investigate the expression patterns of TTYH2 in the human colon cancer and colon cancer cell lines and to evaluate the inhibitory effect of small interfering RNA (siRNA) on the expression of TTYH2 in colon cancer cell ***: We investigated the expression patterns of TTYH2 in colon cancer, adjacent non-tumorous colon mucosa, and cancer cell lines (DLD-1, caco-2, and Lovo) by RT-PCR. Furthermore, a siRNA plasmid expression vector against TTYH2 was constructed and transfected into DLD-1 and Caco-2 with LipofectamineTM 2000. The down regulation of TTYH2 expression was detected by RT-PCR and the role of siRNA in inducing cell proliferation and cell aggregation was evaluated by MTT and aggregation ***: TTYH2 gene expression in colon cancer tissue was significantly up-regulated compared with normal colonic mucosa (1.23 ± 0.404 vs 0.655 ± 0.373, P = 0.0103). Colon cancer derived cell lines including DLD-1, Caco-2, and Lovo also expressed high levels of TTYH2. In contrast, transfection with siRNA-TTYH2 signifi cantly inhibited both proliferation and scattering of these cancer cell ***: The present work demonstrates, for the fi rst time, that the TTYH2 gene expression is signifi cantly up-regulated in colon cancer. The TTYH2 gene may play an important role in regulating both proliferating andmetastatic potentials of colorectal cancer.

关键词： TTYH2 Colorectal carcinoma Cell proliferation Cell aggregation

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Medial Longitudinal Arch Pad Influences Landing Control of the Lower Limbs during Single-Leg Jump-Landing

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Health 2020年第12期12卷 1610-1619页

作者： Yuichi Takata Mitiru Sugimoto Koji Iwamoto Isamu Kitsunai Kyoji Sugiyama kazushi kimura Department of Physical Therapy Faculty of Human Science Hokkaido Bunkyo University Eniwa Japan Department of Physical Therapy Ibaraki Prefectural University of Health Sciences Ibaraki Japan School of Comprehensive Rehabilitation Osaka Prefecture University Osaka Japan

Background: Arch support has the effect of maintaining arch and correcting alignment, and it is broadly used for the prevention of sports impediment and treatment of athletes with lowered MLA and foot problems. The fact that the morphological change of MLA damages balance sense and postural control, it was reported that the insole supporting the arch of MLA improved postural balance. There are several studies regarding the effects of arch support;however, its effects on landing control have not been clarified. Therefore, in our research, we discussed the effect of MLA support for landing control, using lower limb dynamic alignment and the moment during landing as indexes. Methods: This study measured the landing motion to be evaluated was to jump from a platform with a height of 30 cm by taking-off with a single foot, and landing on a single foot on a floor reaction force gauge placed ahead and stay still for three seconds for the subjects were 13 healthy females. A soft 6 mm Boron sheet cut in the size of 9 × 3.5 cm, applied with double-sided tape (MLA pad) was used for arch support (hereafter referred to as “pad”). For the lower limb evaluation, an 8-camera with a three-dimensional behavioral analyzer (CORTEX, NAC product, sampling frequency: 120 Hz) and a floor reaction force gauge (AMTI product, sampling frequency: 1000 Hz) were used. Ten successful jump-landing tests for each limb were used for further analyses using Visual 3D software (Cmotion Inc., Kingston, Canada). Analysis objects were knee joint bending angle and valgus angle during landing;knee joint maximum bending angle;bending knee joint valgus angle, hip joint bending angle, adduction angle, ankle joint plantar flexion angle, varus angle at the time of knee joint maximum bending angle;and each joint moment. For statistical processing, the average value of three trials out of five trials was regarded as a representative value. Results: Regarding joint

关键词： Medial Longitudinal Arch Pad Single-Leg Jump-Landing Landing Control

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