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Expression of adhesion molecules on mature cholangiocytes in canal of Hering and bile ductules in wedge biopsy samples of primary biliary cirrhosis

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World Journal of Gastroenterology 2005年第28期11卷 4382-4389页

作者： Hiroaki Yokomori Masaya Oda Mariko Ogi Go Wakabayashi Shigeyuki Kawachi kazunori Yoshimura Toshihiro Nagai Masaki Kitajima Masahiko Nomura Toshifumi Hibi Department of Internal Medicine Kitasato Institute Medical Center Hospital Saitama 364-8501Japan Organized Center of Clinical Medicine International University of Health and Welfare Tokyo 107-0052Japan Laboratory of Pathology Kitasato Institute Medical Center Hospital Saitama 364-8501Japan Department of Surgery School of Medicine Keio UniversityTokyo 160-0016Japan Physiology Saitama Medical School Saitama 350-0495Japan Electron Microscopy Laboratory School of Medicine Keio UniversityTokyo 160-0016Japan Department of Internal Medicine School of Medicine Keio UniversityTokyo 160-0016Japan

AIM：To examine the expression of intercellular adhesion molecule-1 （ICAM-1） and lymphocyte function-associated antigen-1（LFA-1）expression on canals of Hering （COH）and bile ductules associated with the autoimmune process of bile duct destruction in primary biliary cirrhosis（PBC）．METHODS：Ten wedged liver biopsies of PBC（five cases each of stages 2 and 3）were studied. The liver specimens were processed for transmission electron microscopy. Immunohistochemistry was performed using anti-ICAM-1 and anti-LFA-1 mouse *** situ hybridization was done to examine the messenger RNA expression of ICAM-1 in ***-embedded sections using peptide nucleic acid probes and the catalyzed signal amplification （CSA）***-silver staining for electron microscopy was Derrormed using anti-ICAM and anti-LFA-1 mouse *** immunogold particles on epithelial cells of bileductules and cholangiocytes of CoH cells were counted and analyzed *** blotting was performed to confirm ICAM-1 protein expression．RESULTS：In liver tissues of PBC ***-stochemistry showed aberrant ICAM-1 expression on the plasma membrane of epithelial cells lining bile ductules，and also on mature cholangiocytes but not on hepatocytes in ***-1-positive lymphocytes were closely associated with epithelial cells in bile ***-1 expression at protein level was confirmed by Western *** situ hybridization demonstrated ICAM-1 mRNA expression in bile ductules and LFA-1 mRNA in lymphocytes infiltrating the *** immunoelectron microscopy，ICAM-1 was demonstrated on the basal suface of epithelial cells in bile ductules and on the luminal surfaces of cholangiocytes in damaged *** with intermediate morphology resembling progenitor cells in Coil were not labeled with ICAM-1 and LFA-1．CONCLUSION：De novo expression of ICAM-1 both on mature cholangiocytes in COH and epithelial cells in bile ductules in PBC implies that lymphocyte-indu

关键词： Primary biliary cirrhosis Canal of Hedng Small bile ductile ICAM-1 LFA-1 Immunohistochemistry Western blot Immunogold electron microscopyPrimary biliary cirrhosis Canal of Hedng Small bile ductile ICAM-1 LFA-1 Immunohistochemistry Western blot Immunogold electron microscopy

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Easy MPE:Extraction of Quality Microplot Images for UAV-Based High-Throughput Field Phenotyping

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Plant Phenomics 2019年第1期1卷 30-38页

作者： Léa Tresch Yue Mu Atsushi Itoh Akito Kaga kazunori Taguchi Masayuki Hirafuji Seishi ninomiy. Wei Guo International Field Phenomics Research Laboratory Institute for Sustainable Agro-Ecosystem ServicesGraduate School of Agricultural and Life SciencesThe University of TokyoTokyoJapan 2Montpellier SupAgroMontpellierFrance 3Plant Phenomics Research CenterNanjing Agricultural UniversityNanjingChina 4Memuro Upland Farming Research StationHokkaido Agricultural Research CenterNational Agriculture and Food Research OrganizationHokkaidoJapan 5Institute of Crop ScienceNational Agriculture and Food Research OrganizationTsukuba CityIbarakiJapan

Microplot extraction(PE)is a necessary image processing step in unmanned aerial vehicle-(UAV-)based research on breeding *** present,it is manually using ArcGIS,QGIS,or other GIS-based software,but achieving the desired accuracy is *** therefore developed an intuitive,easy-to-use semiautomatic program for MPE called Easy MPE to enable researchers and others to access reliable plot data UAV images of whole fields under variable field *** program uses four major steps:(1)binary segmentation,(2)microplot extraction,(3)production of∗.shp files to enable further file manipulation,and(4)projection of individual microplots generated from the orthomosaic back onto the raw aerial UAV images to preserve the image *** rows were successfully identified in all trial *** performance of the proposed method was evaluated by calculating the intersection-over-union(IOU)ratio between microplots determined manually and by Easy MPE:the average IOU(±SD)of all trials was 91%(±3).

关键词： enable image union

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Insulin Release from the Beta Cells in Acatalasemic Mice Is Highly Susceptible to Alloxan-Induced Oxidative Stress

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Journal of Diabetes Mellitus 2015年第2期5卷 81-89页

作者： kazunori Takemoto Wakana Doi Ken Kataoka Kohji Ishihara Da-Hong Wang Hitoshi Sugiyama Noriyoshi Masuoka Medical Science Education Center Okayama University of Science Okayama Japan Department of Life Science Faculty of Science Okayama University of Science Okayama Japan Department of Biochemistry Faculty of Science Okayama University of Science Okayama Japan Department of Medicine and Clinical Science Graduate School of Medicine Dentistry and Pharmaceutical Sciences Okayama University Okayama Japan

Background: Catalase deficiency (acatalasemia) is sensitive to alloxan, and the administration to acatalasemic mice develops hyperglycemia under mild conditions. However, the mechanism is still poorly understood. Methods: Alloxan was used to induce the oxidative stress and intraperitoneally administered to acatalasemic and normal mice. The blood samples of these mice after 1, 3, 5 and 7 days were examined. The pancreatic islets 7 days after alloxan administration were isolated, and the insulin released under 3 mM and 20 mM glucose was examined. Results: After alloxan administration, increase of oxidative markers in blood and pancreatic apoptosis in acatalasemic mice were observed immediately. Insulin in blood was lowered after 3 days, and the insulin in acatalasemic mice was lower than that in normal mice. Hyperglycemia in the acatalasemic mice was observed after 3 days. The pancreatic islets after 7 days were isolated. A reduction of the insulin released from the islets under glucose stimulation was observed. The stimulation indexes of the normal and acatalasemic mice were 1.4 ± 0.6 and 0.7 ± 0.3, respectively. Conclusions: Alloxan induced a deterioration of glucose-dependent insulin secretion ability from the islets, and the deterioration mostly contributed to hyperglycemia, rather than apoptosis.

关键词： Alloxan Hyperglycemia Acatalasemia Insulin Release Diabetes Beta Cells

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家具·空间艺术

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现代装饰（家居） 2012年第11期 132-136页

作者： kazunori Matsumura Danya

对Kazurori而言,"Firm. by kazunori Matsumura"不仅仅是一个设计机构,它更像是一个具有创造性的设计项目。kazunori透过家具设计、空间设计与艺术创造三者的融合,用三维立体的方式将"Flrm"的设计精髓加以展现,并注重人与自然的和谐关... 详细信息

对Kazurori而言,"Firm. by kazunori Matsumura"不仅仅是一个设计机构,它更像是一个具有创造性的设计项目。kazunori透过家具设计、空间设计与艺术创造三者的融合,用三维立体的方式将"Flrm"的设计精髓加以展现,并注重人与自然的和谐关系。无论是家具作品WAFFT Stool所呈现的三维扭曲支架结构,

关键词：空间艺术艺术创造设计机构三维立体支架结构人与自然设计过程环境设计陶瓷材质弯曲工艺

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Clinical Observation and Proposed Classification of Vitiliginous Patches by a Wood’s Lamp

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Journal of Cosmetics, Dermatological Sciences and Applications 2020年第4期10卷 204-211页

作者： Yuka Kimura Atsushi Tanemura Yukiho Kurosaki Madoka Takafuji kazunori Yokoi Eiji Kiyohara Noriko Arase Manabu Fujimoto Department of Dermatology Osaka University Graduate School of Medicine Suita Japan

Although vitiligo lesion especially in static state is characterized as sharply demarcated and complete depigmented macule, we encounter patients who have various manners of hypopigmented lesions. We examined the 81 lesions using the newly released Wood’s lamp (Woody®) and investigated whether or not vitiliginous lesions could be uniformly classified under Wood’s lamp illumination and also this classification helped to estimate the tendency of repigmentation after treatment. As result, the vitiliginous lesions were categorized into 4 types on intra- and peri-lesions prior to treatment by using the Wood’s lamp. The inside and border of the lesions were classified as follows: clear white, faint, multi-dot, and perifollicular for the inside, and sharp, blunt, confetti, and trichrome for the border. Suggestive residual pigmentation was detected in 73.6% of patients at the first visit and repigmentation was observed in 67.9% of patients at least 3 months after treatment. Lesions with the “clear white” inside pattern showed significantly lower repigmentation frequency in 38.5% of patients compared to others. The borders with 4 enlarged lesions were composed of 3 of confetti-type and one of sharp-type. This preliminary study demonstrated that detailed observation with a Wood’s lamp could be the basis to classify vitiliginous lesions and might be useful for predicting not only disease progression but also repigmentation prior to treatment.

关键词： Vitiligo Wood’s Lamp Classification Repigmentation Clinical Course

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Analysis of Degenerative Discs in Lumbar Spondylolisthesis Using MRI T2 Mapping

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Open Journal of Radiology 2012年第3期2卷 77-80页

作者： Tsuneo Takebayashi Hiroyuki Takashima Mitsunori Yoshimoto Yoshinori Terashima Hajime Tsuda kazunori Ida Toshihiko Yamashita Department of Orthopedic Surgery School of Medicine Sapporo Medical University Sapporo Japan

Background: Spinal instability, including lumbar degenerative spondylolisthesis (DS), mainly results from degeneration of intervertebral discs (IVD) and the facet joints (FJ). Characterization of the relationship between IVD degeneration in cases of lumbar degenerative spondylolisthesis (DS), and T2 values may be useful for accurate noninvasive evaluation and subsequent treatment. Thus, the goal of this study was to measure T2 values of IVDs in cases with (DS) and without (NS) spondylolisthesis, and to characterize changes of IVDs in DS. Methods: A total of 40 subjects who presented with L4 spondylolisthesis comprised the DS group. Another 40 subjects who did not have lumbar spondylolisthesis, constituted the NS group. T2 values of IVDs were measured and compared in these groups. Results: T2 values for IVDs tended to be lower in the DS group than in the NS group, and these values were significantly different (p < 0.01) within the anterior annulus fibrosus (AF). No significant differences in T2 values between Meyerding grades I and II were observed in any areas of IVDs. Conclusions: It is speculated that in the early stages, the degeneration of the anterior AF develops and is related to the onset of lumbar spondylolisthesis, while in later stages, degeneration of facet joints influences the progression of spondylolisthesis.

关键词： Degenerative Spondylolisthesis Intervertebral Disc T2 Mapping

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Not Follicular but Dermal Melanocyte Precursors Are Histopathologically Retained in Vitiligo

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Journal of Cosmetics, Dermatological Sciences and Applications 2023年第1期13卷 38-44页

作者： Atsushi Tanemura Ryoko Kawabe Yuka Sudo kazunori Yokoi Takanobu Kabasawa Mitsuru Futakuchi Tamio Suzuki Manabu Fujimoto Department of Dermatology School of Medicine Osaka University Graduate Suita Osaka Japan Department of Pathology Faculty of Medicine Yamagata University Yamagata Japan Department of Dermatology Faculty of Medicine Yamagata University Yamagata Japan

Although perifollicular repigmentation in the vitiligo lesions is owing to activation of follicular melanocyte stem cells and/or precursor cells followed by supplying matured melanocytes, the underlying mechanism of diffuse repigmentation on the whole vitiligo surface remains still unknown. In addition to the presence of remaining melanocytes, it is conceivable that dermal melanocyte precursor cells contribute to induce diffuse repigmentation after treatment. Therefore, we investigated here whether dermal and follicular melanocyte precursor cells were reduced or not in vitiligo lesions. We performed an immunostaining for Nestin and p75NGFR as dermal melanocyte precursor cells and MITF/Fzd4 as follicular melanocyte precursor cells and compared the positive cells number between lesions and non-lesions (n = 11). Although MITF+/Fzd4+ cells in the hair follicle were significantly decreased in number in the lesions, Nestin+ and p75NGFR+ cells were not. This result indicates that dermal precursor cells could be retained in the vitiligo lesions but be disturbed to differentiate into matured melanocytes.

关键词： Melanocyte Precursor Cells p75NGFR Nestin Frizzled4 Vitiligo

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Lead Ion Uptake By Sodium Alginate And Calcium Alginate Film:A Comparison Study

Lead Ion Uptake By Sodium Alginate And Calcium Alginate Film...

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2012 International Congress on Informatics,Environment,Energy and Applications(IEEA 2012)

作者： Armando Gomez Kazimierz Wrobel Katarzyna Wrobel Sato kazunori Teoh Wah Tzu Department of Chemistry University of GuanajuatoL.de Retana No.5Guanajuato 36000Mexico Department of Environmental Engineering Nagaoka University of TechnologyNagaokaNiigata 9402188Japan

Lead ion (Pb(II)) uptake from aqueous solution by two different alginate compounds was investigated. Sodium alginate (Na-Alg) and calcium alginate (Ca-Alg) films were successfully fabricated from the commercially available sodium alginate powder. The Pb(II) uptake performance by Na-Alg and CaAlg films was evaluated against time in a 10 mM Pb(II) solution’s batch sorption system. Ca-Alg film was consistently showing a better Pb(II) uptake performance as compared to Na-Alg film within the investigated duration. Further evaluation on Ca-Alg film was carried out at lower Pb(II) concentrations, i.e. 1 mM and 0.1 mM, the Ca-Alg film showed the best performance in 0.1 mM Pb(II) solution at a uptake rate higher than 98%. The Pb(II) uptake performance of Ca-Alg film was also compared with that of the conventionally fabricated Ca-Alg beads. This result indicates that with the same amount of Ca-Alg used, the film shows approximately three time better Pb(II) uptake performance compared to the beads. The results reported herein indicates that Ca-Alg compound is an effective and efficient biosorbent, furthermore the Pb(II) uptake performance is influenced by the sample fabrication method as well, i.e. film versus bead, which contributed to the overall surface area.

关键词： alginate films lead uptake sorption

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The Reproducibility of Patient Setup for Head and Neck Cancers Treated with Image-Guided and Intensity-Modulated Radiation Therapies Using Thermoplastic Immobilization Device

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International Journal of Medical Physics, Clinical Engineering and Radiation Oncology 2013年第4期2卷 117-124页

作者： Akihiro Nakata Kunihiko Tateoka kazunori Fujimoto Yuichi Saito Takuya Nakazawa Tadanori Abe Masaki Yano Koichi Sakata 1Department of Medical Physics Graduate School of Medicine Sapporo Medical University Sapporo Japan 4Radiation Therapy Research Institute Social Medical Corporation Teishinkai Sapporo Japan Department of Medical Physics Graduate School of Medicine Sapporo Medical University Sapporo Japan Department of Medical Physics Graduate School of Medicine Sapporo Medical University Sapporo Japan Department of Radiology Sapporo Medical University Sapporo Japan Department of Medical Physics Graduate School of Medicine Sapporo Medical University Sapporo Japan Nikko Memorial Hospital Muroran Japan

The reproducibility of patient setup is an important issue for head and neck cancers treated with intensity-modulated radiation therapy (IMRT). In this study, an image-guided radiation therapy (IGRT) system has been used to minimize the uncertainty of patient setup while standard thermoplastic masks were used to provide adequate immobilization for the head and neck. However, they do not provide sufficient immobilization of the shoulders, which is an important requirement in comprehensive nodal irradiation. Therefore, we investigated the setup and rotational shifts in head and neck cancer patients undergoing IMRT for which this immobilization device had been used together with an IGRT system. The setup and rotational shifts of patients were analyzed using the ExacTrac X-ray 6D IGRT system. The patients were classified as having head and neck tumors in the upper or lower regions. The upper neck nodes included lymph nodal level II while the lower neck nodes included lymph nodal levels III and IV. Clinical data from 227 treatment sessions of 12 head and neck cancer patients were analyzed. The random translational error in inter-and intra-fraction errors of the anterio-posterior (AP) direction might influence the rotational errors of pitch and roll in the upper region. At the same time, the random translational error in the inter-and intra-fraction errors of the AP direction might influence the rotational error of roll in the lower region. We believe that these random translational errors should be considered during treatment. We found variability in random translational errors for different regions in the anatomy of head and neck cancer patients due to rotational shifts. Depending on the location of the primary lesion or the selected nodal treatment targets, these relative positional variations should be considered when setup and rotational shifts are corrected with IGRT systems before treatment.

关键词： IMRT IGRT Radiation Therapy Immobilization Head and Neck Cancer

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Clinical Utility of Molecular Diagnosis of Blood Stream Infections in Allogeneic Hematopoietic Stem Cell Transplantation Recipients with Hematologic Malignancies

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Advances in Microbiology 2019年第12期9卷 971-982页

作者： Atsushi Fujieda kazunori Nakase Akiko Nakamura Kohshi Ohishi Yuka Sugimoto Fumihiko Monma Masahiro Masuya Naoyuki Katayama Department of Hematology and Oncology Mie University Hospital Tsu Japan Cancer Center Mie University Hospital Tsu Japan Central Clinical Laboratories Mie University Hospital Tsu Japan Transfusion Service Mie University Hospital Tsu Japan

Blood stream infections (BSIs) are a serious problem in patients with hematologic malignancies receiving allogeneic hematopoietic stem cell transplantation (ASCT). We evaluated the clinical utility of molecular diagnosis for the management of BSIs in such patients. We prospectively performed a polymerase chain reaction (PCR) analysis of microbial DNA in blood samples from 10 consecutive patients with hematological malignancies at least once a week for one month after ASCT. In total, 51 and 54 samples were analyzed by bacterial and fungal PCR assays, respectively. Bacteria were detected in 24 samples from 8 patients by PCR, but in only 2 samples from one patient by blood culture. Notably, the bacteria detected in at least half of the 24 samples were considered to have originated from the oral cavity. Fungi were detected in 5 samples from 3 patients by PCR, but not by blood culture. Most cases with positive PCR results were manageable with empirical antimicrobial therapy without disclosure of DNA data. Our DNA analyses did not directly contribute to management of BSIs, but did provide valuable microbiological evidence for the patients. Additionally, oral management appears to require a critical re-evaluation to reduce the occurrence of BSIs in ASCT recipients.

关键词： Allogeneic Hematopoietic Stem Cell Transplantation Blood Stream Infection PCR Analysis

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