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Biological Evaluation of Tissue-Engineered Cartilage Using Thermoresponsive Poly(N-isopropylacrylamide)-Grafted Hyaluronan

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Journal of Biomaterials and Nanobiotechnology 2012年第1期3卷 1-9页

作者： kazuaki muramatsu Mika Ide Fujio Miyawaki Division of Life Science and Engineering School of Science and Engineering Tokyo Denki University Saitama Japan

In order to contribute to the development of minimally invasive surgery techniques for autologous chondrocyte implantation, a novel self-assembling biomaterial consisting of thermoresponsive poly(N-isopropylacrylamide)-grafted hyaluronan (PNIPAAm-g-HA) has been synthesized as an injectable scaffold for cartilage tissue engineering. The aim of this study was to investigate the efficacy and cytocompatibility of PNIPAAm-g-HA to normal chondrocytes by using reverse transcription-polymerase chain reaction (RT-PCR) analysis and histochemical staining in preliminary in vitro and in vitro experiments. Hematoxylin and eosin staining showed homogeneous distribution of cells in the PNIPAAm-g-HA hydrogel in 3-dimensional in vitro cultivation. Alcian blue staining also indicated that abundant extracellular matrix formation, including acidic glycosaminoglycans, occurred in tissue-engineered cartilage over time in vitro. Cartilage-related gene expression patterns, which were tested in rabbit normal chondrocytes embedded in the hydrogel, were almost maintained for 4 weeks. Transforming growth factor-β1 (TGF-β1) stimulation enhanced the expression of SRY-related HMG box-containing gene 9 (Sox9) and type X collagen genes suggesting promotion of chondrogenic differentiation. Histochemical evaluation showed neocartilage formation following subcutaneous implantation of the chondrocyte-gel mixture in nude mice. Furthermore, TGF-β1 stimulation promoted production and maturation of the extracellular matrix of the in situ tissue engineered hyaline cartilage. These data suggested that PNIPAAm-g-HA could be a promising biomaterial, i.e., a self-assembling and injectable scaffold for cartilage tissue engineering.

关键词： Cartilage Tissue Engineering Hyaluronan Self-Assembly Thermally Responsive Material

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Poly(N-isopropylacrylamide-co-N-tert-butylacrylamide)- grafted hyaluronan as an injectable and self-assembling scaffold for cartilage tissue engineering

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Journal of Biomedical Science and Engineering 2012年第11期5卷 639-646页

作者： kazuaki muramatsu Yuji Saito Takuya Wada Hiroyuki Hirai Fujio Miyawaki Division of Life Science and Engineering School of Science and Engineering Tokyo Denki University Saitama Japan

Novel poly(N-isopropylacrylamide-co-N-tert-butylacrylamide)-grafted hyaluronan [P(NIPAAm-co-NtBAAm)-g-HA] has been developed as a modified derivative to improve phase-transition characteristics of PNIPAAm-g-HA, which has a lower critical solution temperature (LCST) of approximately 32°C. This promising self-assembling biomaterial has potential as an injectable scaffold for in situ cartilage tissue engineering. LCST of the P(NIPAAm-co-NtBAAm)-g-HA decreased to approximately 3.6°C compared to that of the original PNIPAAm-g-HA. This modification enabled self-assembly at body temperatures lower than the temperature of the parental PNIPAAm-g-HA molecule. Cytotoxicity and acute systemic toxicity assays revealed that P(NIPAAm-co-NtBAAm)-g-HA was not hazardous. The DNA content of chondrogenic differentiated mesenchymal stem/stromal cells (MSCs) embedded in the gels was higher than that of biomaterial-free aggregates during the culture periods. Cartilage-related genes were also expressed in chondrogenic differentiated MSCs embedded in the P (NIPAAm-co-NtBAAm)-g-HA hydrogel. Specifically, an increased expression of SRY-related HMG box-containing gene 9 (Sox9) observed in the hydrogel group compared to controls. These data suggest that P(NIPAAm-co-NtBAAm)-g-HA is a promising injectable scaffold with thermoresponsive properties suitable for in situ cartilage tissue engineering.

关键词： Thermally Responsive Material Cartilage Tissue Engineering Biocompatibility Mesenchymal Cells

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Effect of the Suppression of Cariesitic Bacterial Growth and Biofilm Formation Using Hydrogen/Free Chlorine Mixed Water Produced by an Electrolysis-Type Hydrogen Generator

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Health 2019年第2期11卷 142-151页

作者： Ryusuke Suzuki Rin Kaneko Masaru Nakano Masato Kanda Yuji Sebata Shota Motegi Tomoko Abe kazuaki muramatsu Division of Life Science and Engineering School of Science and Engineering Tokyo Denki University Saitama Japan SHEM Corporation Tokyo Japan

OBJECTS: Hydrogen has been shown to possess antibacterial effects at high concentrations. In addition, chlorine has a strong bactericidal effect even at low concentrations. Electrolysis is a way to simultaneously generate these two components. However, the concentration of hydrogen/free chlorine mixed water generated through electrolysis decreases quickly. It is predicted that the concentration of hydrogen/free chlorine mixed water will vary depending on the quality of water used. After investigating the optimum generation conditions, the effects of the most stable concentration of hydrogen/free chlorine mixed water on carious tooth fungus were evaluated in vitro. Thus, in this experiment, our goal was to evaluate the effects of hydrogen/free chlorine mixed water on oral bacteria. MATERIALS AND METHODS: Using a device that generates hydrogen/free chlorine through electrolysis, the differences in the concentrations of hydrogen and free chlorine based on electrolysis time were evaluated using tap water. Additionally, various concentration changes due to electrolysis time on the hydrogen/free chlorine mixed water were evaluated. Distilled tap water as a control group, hydrogen/free chlorine mixed water, and commercially available mouthwash were added for 1 minute to cultured Streptococcus mutans and then rinsed out with the culture medium. Bacterial growth (600 nm) and biofilm formation (590 nm) were measured at 3 and 6 hours after the addition of the medium. RESULTS: The concentration of hydrogen/chlorine mixed water produced by electrolysis varied depending on electrolysis time and the water used. The inhibitory effect of bacterial growth was enhanced depending on the chlorine concentration. Regarding the inhibitory effect on biofilm formation, only the mixed water of hydrogen/free chlorine concentration (500 ppb - 1.0 mg/L) had a suppressing effect after 6 hours. CONCLUSION: It was suggested that hydrogen/chlorine mixed water can be easily produced by electrolysis a

关键词： Hydrogen Chlorine Bactericidal Capacity Bacteria Effect

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Mission of the National Cancer Center Hospital in Japan to promote clinical trials for precision medicine

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Cancer Biology & Medicine 2024年第1期21卷 1-3页

作者： kazuaki Shimada Kenichi Nakamura Noboru Yamamoto Department of Hepatobiliary and Pancreatic Surgery National Cancer Center HospitalTokyo 104-0045Japan Clinical Research Support Offce National Cancer Center HospitalTokyo 104-0045Japan Department of Experimental Therapeutics National Cancer Center HospitalTokyo 104-0045Japan

Precision medicine is a growing field *** its potential benefit to many patients,several major obstacles must be overcome before precision medicine can be more widely used in clinical *** main obstacles are associated... 详细信息

关键词： clinical Cancer overcome

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A dynamic database of solid-state electrolyte(DDSE)picturing all-solid-state batteries

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Nano Materials Science 2024年第2期6卷 256-262页

作者： Fangling Yang Egon Campos dos Santos Xue Jia Ryuhei Sato kazuaki Kisu Yusuke Hashimoto Shin-ichi Orimo Hao Li Advanced Institute for Materials Research(WPI-AIMR) Tohoku UniversitySendai980-8577Japan Key Laboratory of Radiation Physics and Technology Ministry of EducationCollege of PhysicsSichuan UniversityChengdu610065People's Republic of China Institute for Materials Research(IMR) Tohoku UniversitySendai980-8577Japan Tohoku Forum for Creativity Tohoku UniversitySendai980-8577Japan

All-solid-state batteries(ASSBs)are a class of safer and higher-energy-density materials compared to conventional devices,from which solid-state electrolytes(SSEs)are their essential *** date,investigations to search for high ion-conducting solid-state electrolytes have attracted broad ***,obtaining SSEs with high ionic conductivity is challenging due to the complex structural information and the less-explored structure-performance *** provide a solution to these challenges,developing a database containing typical SSEs from available experimental reports would be a new avenue to understand the structureperformance relationships and find out new design guidelines for reasonable ***,a dynamic experimental database containing>600 materials was developed in a wide range of temperatures(132.40–1261.60 K),including mono-and divalent cations(e.g.,Li^(+),Na^(+),K^(+),Ag^(+),Ca^(2+),Mg^(2+),and Zn^(2+))and various types of anions(e.g.,halide,hydride,sulfide,and oxide).Data-mining was conducted to explore the relationships among different variates(e.g.,transport ion,composition,activation energy,and conductivity).Overall,we expect that this database can provide essential guidelines for the design and development of high-performance SSEs in ASSB *** database is dynamically updated,which can be accessed via our open-source online system.

关键词： Solid-state electrolyte(SSE) All-solid-state battery(ASSB) Ionic conductivity Dynamic database Machine learning

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Co-infection with Neisseria mucosa in a patient with tuberculous otitis media

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Journal of Otology 2024年第1期19卷 1-4页

作者： Tatsuya Hioki kazuaki Soejima Yuki Goto Makoto Sugiura Takumi Umemura Yoshimi Ishihara Yoshikazu Mutoh Daisuke Sakanashi Hiroshige Mikamo Department of Clinical Laboratory Tosei General Hospital160 Nishioiwake-choSetoAichi489-8642Japan Department of Respiratory Medicine and Allergy Tosei General Hospital160 Nishioiwake-choSetoAichi489-8642Japan Department of Otolaryngology Tosei General Hospital160 Nishioiwake-choSetoAichi489-8642Japan Department of Infection Control Team Tosei General Hospital160 Nishioiwake-choSetoAichi489-8642Japan Department of Infectious Diseases Tosei General Hospital160 Nishioiwake-choSetoAichi489-8642Japan Department of Infection Prevention and Control Aichi Medical University Hospital1-1 YazakokarimataNagakuteAichi480-1195Japan Department of Clinical Infectious Diseases Aichi Medical University1-1 YazakokarimataNagakuteAichi480-1195Japan

Tuberculous otitis media(TOM) is a rare manifestation caused by Mycobacterium tuberculosis with low incidence rates among extrapulmonary tuberculosis cases. Diagnosis is often delayed because of the presence of several clinical manifestations and the high prevalence of secondary bacterial infections. Few reports have attributed secondary bacterial infections in patients with TOM to commensal Neisseria. Thus, understanding the pathogenic mechanisms and clinical features of commensal Neisseria is important, considering its recent presentation as an infection-causing pathogen. Neisseria mucosa is a commensal inhabitant in humans and is generally considered non-pathogenic but can cause infection in rare cases. Here, we report an atypical secondary infection caused by Neisseria mucosa in an 81-year-old woman with TOM being treated for pulmonary tuberculosis. Direct purulent otorrhea smear microscopy revealed no acid-fast bacilli using Ziehl-Neelsen staining, whereas the phagocytosis of gram-negative cocci by white blood cells was confirmed using Gram staining. Otorrhea culture revealed the growth of N. mucosa. Subsequently, M. tuberculosis infection in the otorrhea was identified using a culture-based method. Vigilance is critical for the early detection of TOM to prevent further complications. This report raises awareness regarding TOM and provides insight into the pathogenicity of N. mucosa in otitis media.

关键词： Commensal neisseria Miliary tuberculosis Mycobacterium tuberculosis Neisseria mucosa Tuberculous otitis media

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Data-Driven Viewpoint for Developing Next-Generation Mg-Ion Solid-State Electrolytes

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电化学（中英文） 2024年第7期30卷 38-49页

作者： Fang-Ling Yang Ryuhei Sato Eric Jianfeng Cheng kazuaki Kisu Qian Wang Xue Jia Shin-ichi Orimo Hao Li Advanced Institute for Materials Research(WPI-AIMR) Tohoku UniversitySendai980-8577Japan College of Physics Sichuan UniversityChengdu610065China Institute for Materials Research(IMR) Tohoku UniversitySendai980-8577Japan College of Materials Science and Engineering Sichuan UniversityChengdu610064China

Magnesium(Mg)is a promising alternative to lithium(Li)as an anode material in solid-state batteries due to its abundance and high theoretical volumetric ***,the sluggish Mg-ion conduction in the lattice of solidstate electrolytes(SSEs)is one of the key challenges that hamper the development of Mg-ion solid-state *** various Mg-ion SSEs have been reported in recent years,key insights are hard to be derived from a single literature ***,the structure-performance relationships of Mg-ion SSEs need to be further unraveled to provide a more precise design guideline for *** this viewpoint article,we analyze the structural characteristics of the Mg-based SSEs with high ionic conductivity reported in the last four decades based upon data mining-we provide big-data-derived insights into the challenges and opportunities in developing next-generation Mg-ion SSEs.

关键词： Data mining Magnesium-ion solid-state electrolytes All-solid-state batteries Magnesium-ion conductivity

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On the Existence of Feller Semigroups with Discontinuous Coefficients

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Acta Mathematica Sinica,English Series 2006年第2期22卷 595-606页

作者： kazuaki TAIRA Institute of Mathematics University of Tsukuba

This paper is devoted to the functional analytic approach to the problem of the existence of Markov processes in probability theory. More precisely, we construct Feller semigroups with Dirichlet conditions for second-order, uniformly elliptic integro-differential operators with discontinuous coefficients. In other words, we prove that there exists a Feller semigroup corresponding to such a diffusion phenomenon that a Markovian particle moves both by jumps and continuously in the state space until it dies at the time when it reaches the boundary.

关键词： Feller semigroup Dirichlet condition VMO function

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On the Existence of Feller Semigroups with Discontinuous Coefficients Ⅱ

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Acta Mathematica Sinica,English Series 2009年第5期25卷 715-740页

作者： kazuaki TAIRA Institute of Mathematics University of Tsukuba

This paper is devoted to the functional analytic approach to the problem of existence of Markov processes with Dirichlet boundary condition, oblique derivative boundary condition and first-order Wentzell boundary condition for second-order, uniformly elliptic differential operators with discontinuous coefficients. More precisely, we construct Feller semigroups associated with absorption, reflection, drift and sticking phenomena at the boundary. The approach here is distinguished by the extensive use of the ideas and techniques characteristic of the recent developments in the Calderon- Zygmund theory of singular integral operators with non-smooth kernels.

关键词： singular integral Feller semigroup elliptic operator with VMO coefficients Wentzellboundary condition

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城市代表文明吗?

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建筑师 2024年第1期 4-10页

作者： [日]村松伸赵齐不详哈尔滨工业大学(深圳) 东京大学生产技术研究所

城市史学在20世纪发展迅速,其背后是将城市与文明相连的巨大推力。经历近代与世界大战,在全球格局的变动之下,我们对城市的定位,从一种特定的社会状态,提升到了城市代表人类文明的高度。然而,城市是否真能代表文明?本文将回顾欧、美、... 详细信息

城市史学在20世纪发展迅速,其背后是将城市与文明相连的巨大推力。经历近代与世界大战,在全球格局的变动之下,我们对城市的定位,从一种特定的社会状态,提升到了城市代表人类文明的高度。然而,城市是否真能代表文明?本文将回顾欧、美、中、日近现代城市史发展中观念演变的脉络,在今日重新思考这一问题。

关键词：城市城市史文明爱国主义

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