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Primary Hyperparathyroidism in a Twin Pregnancy: A Case Report

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Open Journal of Endocrine and Metabolic Diseases 2016年第6期6卷 167-171页

作者： Luis Eduardo Pérez Sánchez Julio César Jordan Balanza Alicia Goya Pacheco P.E.G. de Chaves Rodríguez Manuel barrera Gómez Endocrine Surgery Unit Department of General and Digestive Surgery Hospital Universitario Nuestra Se?ora de Candelaria Tenerife Spain

The primary hyperparathyroidism during pregnancy is an infrequent disorder but it is rarer in a twin gestation. Often it is not suspected because the symptomatology is overlapped with the normal course of pregnancy and it is usually discovered after a routine blood test. Ultrasound is the best option to identify the adenoma, while the gold-standard is technetium-99 m sestamibi but is contraindicated in this situation. The treatment is controversial and depends of the time of the gestation: the medical therapy is accepted in the first and third trimester, and the surgery is reserved for the second trimester, where the side effects are low. We present a case of primary hyperparathyroidism in a twin pregnancy treated by a minimally invasive parathyroidectomy in the second trimester without consequences for both, mother and newborns.

关键词： Hyperparathyroidism Pregnancy Surgery

Histological Analysis and Morphometric Changes after Autologous Fat Grafting in Large Volumes on Pigs

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Journal of Cosmetics, Dermatological Sciences and Applications 2021年第4期11卷 371-380页

作者： Hector Giancarlo Torres-Nuño Walter Angel Trujillo-Rangel Jorge barrera-Alcocer Miriam Méndez del Villar Diana Mercedes Hernández-Corona Sylvia Elena Totsuka-Sutto Leonel García Benavides Institute of Therapeutics and Clinical Experimentation Department of Physiology Health Sciences University Center (CUCS) University of Guadalajara (UDG) Guadalajara Mexico Obesity and Diabetes Research Laboratory Faculty of Chemistry and Biological Sciences Autonomous University of Guerrero (UAGro) Chilpancingo Mexico Multidisciplinary Health Research Center Biomedical Sciences Department Tonala University Center (CUT) University of Guadalajara (UDG) Guadalajara Mexico

Nowadays, fat transfer is an accepted technique that has been popularized in clinical practice, during which large volumes of fat are strategically relocated to increase and improve the contour of the body. In the present study, we investigated the histological and morphometric changes after large volumes of fat were grafted within the subcutaneous cellular tissue. An experimental study was designed using four 3-month-old male pigs. One hundred cc of decanted fat tissue was transferred into the right leg. Volume augmentation achieved by auto lipo-injecting within the subcutaneous cellular tissue persisted up to two months. Although there is a reduction in cell number and size, an increased amount of extracellular matrix and the presence of oily cysts and microcalcifications were observed. Conclusion: The volume augmentation, which is achieved by autologous fat transfer in large quantities in subcutaneous cellular tissue, can remain for months.

关键词： Autologous Fat Transfer Adipose Tissue Transplant Autologous Fat Injection Gluteal Augmentation

Effect of Citric Acid, Phytase and Calcium Levels on the Calcium and Phosphorus Content in Egg: Yolk-Albumen and Shell, Yolk Color and Egg Quality in Diets of Laying Hens

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Food and Nutrition Sciences 2016年第14期7卷 1364-1374页

作者： Lily M. Vargas-Rodríguez Jesús E. Morales-barrera José G. Herrera-Haro Juan Antonio-Bautista Roberto López-Pozos David Hernández-Sánchez Instituto de Recursos Genéticos y Productividad (IREGEP) Colegio de Postgraduados Texcoco México Departamento de Medicina Veterinaria y Zootecnia Universidad Autónoma Metropolitana Xochimilco D.F. México México División de Ingeniería en Procesos Bioalimentarios Universidad Tecnológica de los Valles Centrales de Oaxaca Oaxaca México Licenciatura en Zootecnia Universidad del Mar. Puerto Escondido Oaxaca México

To evaluate the effect of different levels of citric acid (CA) combined with phytase and calcium levels in the diets of laying hens on the calcium and phosphorus (P) contents of eggs: yolk-albumen and shell, yolk color and egg quality. A study was conducted with 24-week-old laying hens fed with a diet based on sorghum and soybean meal with 2.7 Mcal of EM/kg, 15% crude protein, 3.25% calcium and 0.25% available P. The levels of CA, phytase and calcium were 0%, 0.6% and 1.2%;0 and 300 units of phytase (PHYU)/kg;and 3.0% and 3.25%, respectively. Phytase was added as an ingredient into to the diets, contributing 0.1% P and 0.3% calcium. A completely randomized factorial experiment was performed with a 3 × 2 × 2 design and 4 replications. The CA increased (P < 0.05) the P in the shell, and the combination of 1.2% CA, 300 PHYU phytase and 3.25% calcium increased (P < 0.05) the calcium in the eggshell. There was a CA × phytase × calcium interaction (P < 0.05) affecting egg yolk pigmentation;there were no differences in egg quality. It was concluded that adding CA to the diets of laying hens increases eggshell calcium content, and improves the phytase response. 1.2% CA × Phytase × 3.25% calcium significantly increases shell calcium by 0.56 g. Under the conditions of the present investigation, it would imply greater resistance of the eggs to be broken in the handling from the farm to the sales centers and consumer. Phytase interacts negatively with 0.6% CA at low calcium levels to degrade the yellow pigmentation of the yolk.

关键词： Citric Acid Phytase Calcium Laying Hens Eggshell

Antioxidant activity of Bios-p peptide analogue in HEK293T cells and three-dimensional structure prediction

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Advances in Bioscience and Biotechnology 2013年第1期4卷 55-61页

作者： Pamela Fernández Manzano Ignacio Jofré Fernández Fabiola Zambrano Patricia Navarrete Gómez Magdalena Cuevas Troncoso Ximena Romo Marty Andrés ávila barrera Fernando Romero Mejía Center of Neurosciences and Peptides Biology Universidad de La Frontera Temuco Chile Centro de Modelación y Computación Científica Universidad de La Frontera Temuco Chile Departamento de Ciencias Biologicas Facultad de Ciencias Biologicas Universidad Andres Bello Talcahuano Chile

Studies had indicate that excessive production of reactive oxygen species (ROS) affect cellular signaling pathways, which is associated with pathological and physiological conditions such as cancer, diabetes and neurodegenerative diseases In this context, our laboratory has obtained the Bios-p, a ROS modulator, peptide analogue by sequencing from the seed of Bauhinia bauhinoides, which represents the active 12-amino acid, obtained from the inhibitor BbKI protease and we predicted the three-dimensional structure of Bios-p analogue peptide using homology modeling, being patented by the working group of Dr. Maria Luiza Vilela Oliva of UNIFESP, Brazil (a member of our cluster). The protective effect on the viability and antioxidant capacity of Bios-p was studied in HEK 293T cells under oxidative stress induced by hydrogen peroxide (H2O2) using SYTOXGREEN/DHE and luminescence assay. The three-dimensional structure of Bios-p peptide analogue was predicted by homology-based modeling using Modeller9v8. The pretreatment with different concentrations of Bios-p (1 μM - 10 μM) showed an increase of 53.83% ± 3.86% the cellular viability in under oxidative stress compared to control. Furthermore, the results to indicate that HEK293T cells by incubating for 24 h with Bios-p shown a significant decreased of basal extracellular ROS on total cell population in 89.67% ± 0.76%, compared to control in the absence of the analogue. Similarly it is observed that Bios-p has a significant antioxidant effect on extracellular ROS production when cells are subjected to oxidative stress induced by 200 μM H2O2 in 64.37% ± 4.63%, compared to control in absence of H2O2 and Bios-p. These results suggest that Bios-p has potential as antioxidant agent in cells HEK293T under H2O2-induced oxidative stress and that can protect the cells viability as concentration-dependent, and we propose a new biotechnological tool for modulate the ROS production.

关键词： Antioxidant Bios-p Bauhinia bauhinoides

Synthesis and Characterization of Chitosan Nanoparticles Loaded Botanical Extracts with Antifungal Activity on Colletotrichum gloeosporioides and Alternaria species

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Advances in Microbiology 2018年第4期8卷 286-296页

作者： Laura Leticia barrera-Necha Zormy Nacary Correa-Pacheco Silvia Bautista-Banos Mónica Hernández-López Jorge Eduardo Martínez Jiménez Aime Frida Morán Mejía Instituto Politécnico Nacional Centro de Desarrollo de Productos Bióticos MorelosMéxico

In this study, chitosan nanoparticles (CSNPs) and chitosan nanoparticles- botanical extracts: EEA-CSNPs (ethanolic blueberry extract added chitosan nano-particles) and EMN-CSNPs (extract methanol of nanche added chitosan nano-particles) were characterized and evaluated in vitro ongrowth of Alternaria alternata isolated from Fig and Rosemary and Colletotrichum gloeosporioides isolated from Papaya and Soursop. From particle size distribution characteriza-tion, the size of nanoparticles increased after EEA incorporation. On the other hand, the smallest value of Z-average particle size was for the EMN-CSNPs. Zeta potential value decreased for CSNPs and EEA-CSNPs. However, when EMN is incorporated to CSNPs, the value is increased. From the results, it can be seen that the most stable suspension was EMN-CSNPs. After incorporation of Byrsonima crassifolia to CSNPs, no changes were observed in characteristic absorption bands for the FTIR spectra. However, after Vaccinium corymbosum incorporation to the CSNPs, changes were seen. For in vitro evaluation, CSNPs without EEA caused the total germination and sporulation inhibition of A. alternata from Rosemary. Incorporation of EMN to CSNPs improved the control of C. gloeosporioides with amycelial growth inhibition of 79% isolated from papaya and 82% isolated from soursop. In both isolated there were total germination inhibition. Overall, a synergistic effect between the chitosan and EMN was observed.

关键词： Zeta Potential In Vitro Fungi Blueberry Nanche

Limphocutaneous Sporotrichosis Due to Sporothrix schenckii var schenckii: A Relapsing Case after Treatment with Potassium Iodide in Elderly

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Advances in Microbiology 2022年第4期12卷 192-197页

作者： Eder R. Juárez-Durán Sabrina Escandón-Pérez Fritz Cajuste-Sequeira Luis M. Sánchez-Navarro Carlos A. barrera-Ochoa Rigoberto Hernández-Castro Roberto Arenas General Hospital Mexico City Mexico ángeles Lomas Hospital Mexico City Mexico La Habana University Havana Cuba Dermatologist General Hospital Oaxaca Mexico General Hospital Mexico City Mexico Pathogen Ecology Department General Hospital Mexico City Mexico

Sporotrichosis is a subcutaneous mycosis caused by the Sporothrix schenckii complex. It has three classic clinical variants: fixed, lymphangitic, and systemic. Treatment in most cases has been itraconazole or potassium iodide. The aim of this paper is to communicate an unusual relapsing case treated with IK. We report a 73-year-old woman with lymphangitic sporotrichosis, adequate response to treatment with potassium iodide, and recurrence 15 months after. A molecular test was performed through the amplification of a 331 bp fragment of the calmodulin gene. In both infections, the same specie was isolated. The effects of potassium iodide are briefly discussed, and we conclude that the same treatment can be prescribed, if no side effects are observed.

关键词： Sporotrichosis Lymphangitic Sporotrichosis Sporothrix schenckii Recurrence Potassium Iodide

Role of the Zinc Nutritional Status on Main Physiological Bioindicators of the Pecan Tree

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Agricultural Sciences 2017年第12期8卷 1327-1336页

作者： Angelica Anahi Acevedo-barrera Esteban Sanchez Rosa Maria Yanez-Munoz Juan Manuel Soto-Parra Angel Lagarda-Murrieta Vicente de Paul Alvarez-Reyna Eduardo Madero-Tamargo Alejandro Moreno-Resendez Universidad Autonoma Agraria Antonio Narro TorreonMéxico Centro de Investigacion en Alimentacion y Desarrollo A.C.Unidad Delicias DeliciasMéxico Facultad de Ciencias Agrotecnologicas Universidad Autonoma de ChihuahuaChihuahuaMéxico

The purpose of this study was to analyze the effect of Zn nutritional status on the content of pigments, nonstructural carbohydrates, foliage proteins and amino acids of the pecan tree, as possible bioindicators of Zn deficiency. The experimental design was completely random with three Zn nutritional statuses: Normal, Intermediate and Deficient, being selected considering visual symptomatology. On all three nutritional statuses, the concentration of photosynthetic pigments was determined as well as of total soluble proteins and amino acids. Obtained results indicate that Zn deficient nutritional status was characterized by minimum photosynthetic pigment, protein concentrations, maximum amino acid and nonstructural carbohydrate concentrations. While the normal Zn nutritional status presented maximum photosynthetic pigments, proteins concentrations, minimum amino acids and carbohydrate concentrations. On the other hand, intermediate Zn nutritional status was characterized by a medium level between the other two Zn nutritional statuses. Finally, we stress the fact that pigments, proteins, amino acids and nonstructural carbohydrates may be good physiological indicators related to Zn nutritional status on pecan trees.

关键词： Carya illinoinensis Bioindicators Deficiency Nutritional Stress

Fatty Acid Deposition on Broiler Meat in Chickens Supplemented with Tuna Oil

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Food and Nutrition Sciences 2013年第9期4卷 16-20页

作者： Jesus Eduardo Morales-barrera Mariano Jesus Gonzalez-Alcorta Rosa Maria Castillo-Dominguez Omar Francisco Prado-Rebolledo Xochitl Hernandez-Velasco Anita Menconi Guillermo Tellez Billy Marshal Hargis Silvia Carrillo-Dominguez Departamento de Producción Agrícola y Animal Universidad Autónoma Metropolitana México City México Department of Poultry Science University of Arkansas Fayetteville USA. Depto. Nutrición Animal Instituto Nacional de Ciencias Médicas y Nutrición Salvador Zubirán México City México Facultad de Medicina Veterinaria y Zootecnia Universidad de Colima Colima México Facultad de Medicina Veterinaria y Zootecnia Universidad Nacional Autónoma de México México City México Universidad Autónoma Chapingo Estado de México México

The aim of the present study was to evaluate the effect of feeding four levels of tuna oil on performance and fatty acid (FA) profiles of broiler chicken meat. 240 Ross broiler chickens were randomly assigned to 20 pens and divided into four treatments: 0%, 0.75%, 1%, and 1.25% of tuna oil. At 49 days, breast and legs-thighs muscles were processed for FA analysis. Concentrations of FA in legs and thighs meat were significantly higher when compared with breast meat. In both types of meats (breast and legs-thighs), the inclusion of 1.0% or 1.25% of tuna oil in the diet significantly increased (P < 0.05) the concentration of n-3 FA (especially docosahexaenoic and eicosapentaenoic acids). Fat in broiler breast contained a proportion of 29% saturated FA (SFA): 36% monosaturated FA (MFA): 35% polyunsaturated FA (PUFA);while legs and thighs meat had a proportion of 28% SFA: 38% MFA: 33% PUFA. The addition of tuna oil in the broiler diet significantly reduced the deposition of SFA, MFA, and PUFA in breast meat, while in legs and thighs these reductions were less noticeable. The significant reduction in the concentration of n-6 PUFA and the increment of n-3 PUFA were more evident in breast than in legs and thighs, and with the addition of 1% and 1.25% of tuna oil. The results of the present study suggest a difference in FA deposition attributable to supplementation with tuna oil. The addition of tuna oil could be recommendable to increase n-3 PUFA in both broiler chicken breast and legs-thighs meats, providing a healthier and functional chicken meat to consumer.

关键词： n-3 Fatty Acids Broilers Tuna Oil Breast Meat Legs and Thighs Meat

Unilateral Impact of Altered Loading by Changing Teeth Height on the TMJ Fibrocartilage: Disc and Condyle of Wistar Rats

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Microscopy Research 2016年第2期4卷 20-31页

作者： Roxanne M. Olvera-Farias Jose Raymundo Cruz-Perez Rogelio Salinas-Gutierrez Jose Antonio Guerrero-Diaz de Leon Juan B. Kouri-Flores Raul Rosales-Ibañez David Masuoka Ito Alma Lilian Guerrero barrera Departamento de Morfología Universidad Autónoma de Aguascalientes Aguascalientes México Departamento de Infectómica y Patogénesis Molecular CINVESTAV-IPN Mexico City Mexico Departamento de Estadística Universidad Autónoma de Aguascalientes Aguascalientes México Departamento de Estomatología Universidad Autónoma de San Luis Potosí San Luis Potosi México Departamento de Estomatología Universidad Autónoma de Aguascalientes Aguascalientes México Laboratorio de Biología Celular y Tisular Departamento de Morfología Universidad Autónoma de Aguascalientes Aguascalientes México

Temporomandibular joint (TMJ) is sensitive to loading and mechanical stress that provokes morphological changes produced by the impact in the occlusal plane. Here, this impact is evaluated in TMJ articular disc and articular cartilage using an in vivo model of unilateral occlusal plane impact and by analysis of serial tissue sections stained with Hematoxylin-Eosin (H-E) or with Masson trichrome technique. Thus, six groups of 5 Wistar rats (200 - 250 g) are subjected to biomechanical dental stimulation by placing unilateral resin occlusal interference, or unilateral tooth wear made by upper left molars artificial mechanical devastation (1 control and 2 experimental groups for each treatment). Each treatment is evaluated two times at 1 and 15 days post-treatment. By H-E staining, control groups show chondrocytes arrangement as several cord cell groups in comparison with the experimental groups, which show an arrangement in one cord cell along of articular disc. However, this yields no significant difference (p < 0.05) in cell number between control and experimental groups. In contrast, in articular cartilage chondrocytes are random distributed along the superficial zone in control groups, while in experimental groups cell-free regions are observed in superficial zone. An image Blue hue analysis for trichrome stain is performed to quantify collagen;this shows a significant collagen decrease (p < 0.05) in almost all experimental groups compared with the controls. A degenerative process biomechanically induced by unilateral occlusal plane modification, causes cell and tissue changes on the TMJ structures that remain the degenerative changes observed in early osteoarthritis.

关键词： Articular Disc Articular Cartilage Dental Occlusion Collagen Temporomandibular Joint