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Anti-chikungunya activity of luteolin and apigenin rich fraction from Cynodon dactylon

Asian Pacific Journal of Tropical Medicine 2015年第5期8卷 352-358页

作者： Krishnan Saravana Murali Srinivasan Sivasubramanian Savariar Vincent Shanmugaraj Bala Murugan Bupesh Giridaran Sundaram Dinesh Palani Gunasekaran kaveri krishnasamy Ramalingam Sathishkumar Plant Genetic Engineering Lab Department of Biotechnology.Bharathiar University Department of Virology King Institute of Preventive Medicine and Research Centre for Environmental Research and Development and Loyola Institute of Frontier Energy Loyola College Vellore Institute of Technology Plant Genetic Engineering Lab Department of BiotechnologyBharathiar University

Objective:To obtain Iuteolin and apigenin rich fraction from the ethanolic extract of Cynodon dactylon(L.)(***) Pers and evaluate the fraction's cytotoxicity and anti-Chikungunya potential using Vero ***:The ethanolic extract of *** was subjected to silica gel column chromatography to obtain anti-chikungunya virus(CHIKV) *** phase-HPLC and GC-MS studies were carried out to identily the major phytochemicals in the fraction using phylochemical *** and the potential of the fraction against CHIKV were evaluated in vitro using Vero *** in viral replication was assessed by reverse transcriptase-polymerase chain reaction(RT-PCR) after treating the viral infected Vero cells with the ***:Reverse Phase-HPLC and GC-MS studies confirmed the presence of flavonoids,luteolin and apigenin as major phytochemicals in the anti-CHIKV ethanolic fraction of ***- The fraction was found to exhibit potent viral inhibitory activity(about 98%) at the concentration of 50 μg/mL as observed by reduction in cytopathic effect,and the cytotoxic concentration of the fraction was found to be 250 μg/***-PCR analyses indicated that the reduction in viral mRNA synthesis in fraction treated infected cells was much higher than the viral infected control ***:Luteolin and apigenin rich ethanolic fraction from *** can be utilized as a potential therapeutic agent against CHIKV infection as the fraction does not show cytotoxicity while inhibiting the virus.

关键词： Antiviral activity Chikungunya virus Cynodon dactylon Flavonoids Luteolin Apigenin

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