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私藏夏装大公开

国际服装技术 2006年第9期 88-93页

作者： Sayako lto Isao Yamaguchi（摄影） Hirohikoi Eguchi（摄影） katsuyuki Masuda（摄影） Koshi Uchiyama（摄影） Yoshiharu Yagyu（摄影） Yuichi Ohkura（摄影） Hideyuki Watanabe（摄影） Jacky Yu（翻译）

LUCi对于人气模特们的个人夏季时尚装扮十分关注！这次特地邀请了八位活跃于杂志中的模特，身着自己的衣装登场！鲜有机会目睹的，最真实的扮靓技巧在此呈上！

关键词：夏装模特时尚

Fabrication and Evaluation of Bragg Gratings on Optimally Designed Silicon-on-Insulator Rib Waveguides Using Electron-Beam Lithography

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Journal of Semiconductors 2006年第8期27卷 1347-1350页

作者：武志刚张伟刚王志开桂云袁树中董孝义宇高胜之和田恭雄南开大学现代光学研究所天津300071 早稻田大学理工学部

The fabrication of Bragg gratings on silicon-on-insulator （SOI） rib waveguides using electron-beam lithography is presented. The grating waveguide is optimally designed for actual photonic integration. Experimental and theoretical evaluations of the Bragg grating are demonstrated. By thinning the SOl device layer and deeply etching the Bragg grating, a large grating coupling coefficient of 30cm^-1 is obtained.

关键词： integrated optics Bragg grating silicon-on-insulator rib waveguide

大肠埃希菌中疟原虫MSP1-17编码基因蛋白重组及表达

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中国媒介生物学及控制杂志 2007年第5期18卷 390-393页

作者：张庆军陈国英黄希宝由井克之湖北省预防医学科学院武汉430079 日本长崎大学医学部感染与免疫系

目的为免疫原性及疟疾特异性早期诊断研究，以及高效抗红内期疟原虫复合多价基因疫苗的研究提供基础。方法（1）以鼠约氏疟原虫MSP1编码基因为模板，片段自4855到5271共416bp，扩增出PyMSP1 DNA片段，将该片段克隆入质粒pPGEX-2T，构建... 详细信息

目的为免疫原性及疟疾特异性早期诊断研究，以及高效抗红内期疟原虫复合多价基因疫苗的研究提供基础。方法（1）以鼠约氏疟原虫MSP1编码基因为模板，片段自4855到5271共416bp，扩增出PyMSP1 DNA片段，将该片段克隆入质粒pPGEX-2T，构建含有GST编码的质粒pGEX-2T-PyMSP1-17。（2）用上述构建的质粒植入大肠埃希菌DH-5α表达重组蛋白。结果（1）构建出约氏疟原虫裂殖子表面蛋白1融合基因片段真核重组表达质粒。（2）获得MSP1-GST融合蛋白，在大肠埃希菌DH一5a收获到重组的约氏疟原虫裂殖子表面蛋白浓度为46mg／L。（3）原核表达的蛋白产物免疫小鼠后分离血清，采用间接免疫荧光法（IFA）检测疟原虫，滴度在1：400～1：1000之间均可识别IFA血片中的疟原虫。结论设计重组MSP1-17基因在大肠埃希菌中真核系统内能够得到充分表达，并具有免疫原性和抗原性。无论是作为DNA疫苗研究的候选者抑或是制备特异性抗体进行疟疾早期诊断都具有重要的意义；由于该抗原的表达量高、免疫原性强，是目前很有希望的疟疾疫苗候选抗原之一。

关键词：约氏疟原虫裂殖子表面蛋白基因重组

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水处理用聚合物助凝剂及其制造

水处理信息报导 2002年第3期 42-42页

作者： Kataoka,katsuyuki

Repeat endoscopic submucosal dissection for recurrent gastric cancers after endoscopic submucosal dissection

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World Journal of Gastrointestinal Endoscopy 2013年第12期5卷 600-604页

作者： Yuto Shimamura Naoki Ishii Kaoru Nakano Takashi Ikeya Kenji Nakamura Koichi Takagi katsuyuki Fukuda Koyu Suzuki Yoshiyuki Fujita Department of Gastroenterology St. Luke’s International Hospital Department of Pathology St. Luke’s International Hospital

AIM:To clarify the safety and efficacy of repeat endoscopic submucosal dissection(re-ESD)for locally recurrent gastric cancers after ***:A retrospective evaluation was performed of the therapeutic efficacy,complications and followup results from ESD treatment for early gastric cancers in 521 consecutive patients with 616 lesions at ***`s International Hospital between April 2004 and November *** addition,tumor size,the size of resected specimens and the operation time were compared between re-ESD and initial ESD procedures.A flex knife was used as the primary surgical device and a hook knife was used in cases with severe fibrosis in the submucosal *** variables were analyzed using the non-parametric Mann-Whitney U test and are expressed as medians(range).Categorical variables were analyzed using a Fisher’s exact test and are reported as *** significance was defined as a P-value less than ***:The number of cases in the re-ESD group and the initial ESD group were 5 and 611,*** median time interval from the initial ESD to re-ESD was 14(range,4-44 mo).En bloc resection with free lateral and vertical margins was successfully performed in all re-ESD cases without any *** local or distant recurrence was observed during the median follow-up period of 48(range,11-56 mo).Tumor size was not significantly different between the re-ESD group and the initial ESD group(median 22 mm vs 11mm,P=0.09),although the size of resected specimens was significantly larger in the re-ESD group(median 47 mm vs 34 mm,P<0.05).There was a nonsignificant increase observed in re-ESD operation time compared to initial ESD(median 202 min vs 67 min,respectively,P=0.06).CONCLUSION:Despite the low patient number and short follow-up,the results suggest that re-ESD is a safe and effective endoscopic treatment for recurrent gastric cancer after ESD.

关键词： Endoscopic submucosal dissection Recurrent gastric cancer Gastric cancer Endoscopic mucosal resection Therapeutic endoscopy

Endoscopic band ligation for bleeding lesions in the small bowel

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World Journal of Gastrointestinal Endoscopy 2014年第10期6卷 488-492页

作者： Takashi Ikeya Naoki Ishii Yuto Shimamura Kaoru Nakano Mai Ego Kenji Nakamura Koichi Takagi katsuyuki Fukuda Yoshiyuki Fujita Department of Gastroenterology St.Luke’s International Hospital

AIM: To investigate the safety and efficacy of endo-scopic band ligation(EBL) for bleeding lesions in the small ***: This is a retrospective study evaluating EBL in six consecutive patients(three males, three fe-males, 46-86 years of age) treated between May 2009 and February 2014: duodenal vascular ectasia; 1, je-junal bleeding diverticulum; 1, ileal Dieulafoy's lesion; 1 and ileal bleeding diverticula; 3. The success of the initial hemostasis was evaluated, and patients were observed for early rebleeding(within 30 d after EBL), and complications such as perforation and abscess for-mation. Follow-up endoscopies were performed in four ***: Initial hemostasis was successfully achieved with EBL in all six patients. Eversion was not sufficient in four diverticular lesions. Early rebleeding occurred three days after EBL in one ileal diverticulum, and arepeat endoscopy revealed dislodgement of the O-band and ulcer formation at the banded site. This rebleeding was managed conservatively. Late rebleeding occurred in this case(13 and 21 mo after initial EBL), and re-EBL was performed. Follow-up endoscopies revealed scar formation and the disappearance of vascular lesions at the banded site in the case with a duodenal bleeding lesion, and unresolved ileal diverticula in three cases. Surgery or transarterial embolization was not required without any complications during the median follow-up period of 45(range, 2-83) ***: EBL is a safe and effective endoscopic treatment for hemostasis of bleeding lesions in the small bowel.

关键词： Endoscopic band ligation Small bowel Di-verticular hemorrhage Jejunal bleeding Ileal bleeding Therapeutic endoscopy Hemostasis

CD55高表达在分离乳腺癌SP细胞中的作用研究

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中国医科大学学报 2010年第5期39卷 325-327页

作者：徐静娴刘冰阳森井英一青世克之贾心善张劲松中国医科大学附属第四医院眼科中心眼科医院辽宁省晶状体学重点实验室沈阳110005 中国医科大学沈阳110001 大阪大学医学院病态病理教研室日本大阪565-0871 中国医科大学基础医学院病理学教研室沈阳110001

目的探讨应用细胞CD55高表达的特性替代Hoechst33342染色来分离乳腺癌SP细胞的可行性。方法应用Hoechst 33342及抗CD55抗体对两种乳腺癌细胞株进行荧光染色,应用FACS-Vantage和FACSVantageSE分选SP和MP细胞、CD55高表达(CD55hi)和CD55... 详细信息

目的探讨应用细胞CD55高表达的特性替代Hoechst33342染色来分离乳腺癌SP细胞的可行性。方法应用Hoechst 33342及抗CD55抗体对两种乳腺癌细胞株进行荧光染色,应用FACS-Vantage和FACSVantageSE分选SP和MP细胞、CD55高表达(CD55hi)和CD55低表达(CD55lo)细胞,对比2组细胞对血清缺失诱导的凋亡的耐受性及CD55hi和CD55lo细胞的克隆形成能力。结果对分选的CD55hi和CD55lo细胞行Hoechst 33342染色,2组细胞分别位于SP和MP区域中。在血清缺失情况下,SP细胞和CD55hi细胞分别比MP细胞和CD55lo细胞对凋亡刺激具有更高的耐受性。CD55hi细胞较CD55lo细胞具有更强的克隆形成能力。结论细胞CD55高表达的特性可以替代Hoechst33342染色用于分离乳腺癌SP细胞。

关键词： CD55 Hoechst 33342 乳腺癌 SP细胞

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乳腺癌中CD55高表达细胞的生物学特性

中国组织化学与细胞化学杂志 2010年第6期19卷 532-536页

作者：徐静娴姚志斌森井英一贾心善青世克之张劲松中国医科大学附属第四医院眼科中心眼科医院辽宁省高校晶状体重点实验室沈阳110005 大阪大学医学院病态病理教研室中国医科大学基础医学院病理教研室沈阳110001

目的探讨乳腺癌细胞株MCF7中CD55高表达细胞的生物学特性。方法应用抗CD55抗体及Hoechst33342染料对乳腺癌细胞株MCF7细胞进行荧光染色,应用FACS-Vantage和FACS Vantage SE分选CD55hi和CD55lo细胞、SP和MP细胞,对比两组细胞在两种染色... 详细信息

目的探讨乳腺癌细胞株MCF7中CD55高表达细胞的生物学特性。方法应用抗CD55抗体及Hoechst33342染料对乳腺癌细胞株MCF7细胞进行荧光染色,应用FACS-Vantage和FACS Vantage SE分选CD55hi和CD55lo细胞、SP和MP细胞,对比两组细胞在两种染色中的分群所在、对C2-ceramide诱导凋亡的耐受能力、及应用RT-PCR和免疫组化来检测Bcl-2、TMEM23在两组细胞中的表达。结果分选的CD55hi和CD55lo细胞再用Hoechst 33342染色时,分别位于SP和MP区域中。在C2-ceramide存在的情况下,CD55hi细胞比CD55lo细胞对凋亡刺激具有更高的耐受性。在CD55hi和SP细胞中Bcl-2及TMEM23的表达水平分别比在CD55lo和MP细胞中高。结论 CD55高表达细胞具有与癌干细胞相似的生物学特性,CD55可以推测为乳腺癌干细胞的一个表面标记。

关键词：乳腺癌 CD55 癌干细胞 Hoechst 33342

What types of early gastric cancer are indicated for endoscopic ultrasonography staging of invasion depth?

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World Journal of Gastrointestinal Endoscopy 2016年第16期8卷 558-567页

作者： Jiro Watari Shigemitsu Ueyama Toshihiko Tomita Hisatomo Ikehara Kazutoshi Hori Ken Hara Takahisa Yamasaki Takuya Okugawa Takashi Kondo Tomoaki Kono katsuyuki Tozawa Tadayuki Oshima Hirokazu Fukui Hiroto Miwa Division of Gastroenterology Department of Internal Medicine Hyogo College of Medicine Department of Inflammatory Bowel Disease Hyogo College of Medicine

AIM To clarify the diagnostic efficacy and limitations of endoscopic ultrasonography(EUS) and the characteristics of early gastric cancers(EGCs) that are indications for EUS-based assessment of cancer invasion *** We retrospectively investigated the cases of 153 EGC patients who underwent conventional endoscopy(CE) and EUS(20 MHz) before *** We found that 13.7% were "inconclusive" cases with low-quality EUS images, including all nine of the cases with protruded(0-I)-type EGCs. There was no significant difference in the diagnostic accuracybetween CE and EUS. Two significant independent risk factors for misdiagnosis by EUS were identified-ulcer scarring [UL(+); odds ratio(OR) = 4.49, P = 0.003] and non-indication criteria for endoscopic resection(ER)(OR = 3.02, P = 0.03). In the subgroup analysis, 23.1% of the differentiated-type cancers exhibiting SM massive invasion(SM2) invasion(submucosal invasion ≥ 500 μm) by CE were correctly diagnosed by EUS, and 23.1% of the undifferentiated-type EGCs meeting the expanded-indication criteria for ER were correctly diagnosed by *** There is no need to perform EUS for UL(+) EGCs or 0-I-type EGCs, but EUS may enhance the pretreatment staging of differentiated-type EGCs with SM2 invasion without UL or undifferentiated-type EGCs revealed by CE as meeting the expanded-indication criteria for ER.

关键词： Gastric cancer Endoscopic ultrasonography Invasion depth diagnosis Conventional endoscopy Endoscopic submucosal dissection