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e.aluation of Re.l-Time.16S rDNA PCR and Pyrose.ue.cing for Routine.Ide.tification of Bacte.ia in Joint Fluid and Tissue.Spe.ime.s

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Ope. Journal of Me.ical Microbiology 2011年第1期1卷 1-6页

作者： Naomi J. Gadsby Ale. One. Sally-Anne.Phillips Luke.Tysall Ste.fe. J. Bre.sch Hamish Simpson Jayshre. Dave.e.zbie.a Czarniak kate e. templeton Microbiology Department of Laboratory Medicine Royal Infirmary of Edinburgh De.artme.t Orthopae.ic Surge.y Royal Infirmary of Edinburgh

16S rDNA PCR and se.ue.cing are.powe.ful tools for bacte.ial de.e.tion and ide.tification, although the.r routine.use.is not curre.tly wide.pre.d in the.fie.d of clinical microbiology. The.availability of pyrose.ue.cing now make. 16S rDNA assays more.acce.sible.to routine.diagnostic laboratorie., but this approach has had limite. e.aluation in ge.e.al diagnostic practice. In this study we.e.aluate. a re.l-time.16S rDNA PCR and pyrose.ue.cing assay for use.in a routine.microbiology laboratory, by re.rospe.tive.y te.ting joint fluid and joint tissue.spe.ime.s re.e.ve. for conve.tional culture. We.found that use.of the.re.l-time.16S rDNA assay was clinically valuable.in this spe.ime. type.be.ause.it e.able. us to ide.tify a small numbe. of culture.ne.ative.infe.tions. Although faste. and le.s labour-inte.sive. we.found that the.utility of pyrose.ue.cing for pathoge. ide.tification is still hampe.e. by shorte. re.d le.gths compare. to conve.tional (Sange.) se.ue.cing. Combining re.ults from both mole.ular and conve.tional culture.me.hods, bacte.ia we.e.only de.e.te. in 11.8% spe.ime.s in this study. Howe.e., the.de.e.tion rate.was incre.se. to 18.6% if spe.ime.s we.e.only include. from patie.ts with a docume.te. clinical suspicion of infe.tion. In conclusion, while.pyrose.ue.cing had significant advantage. in spe.d and e.se.of-use.ove. conve.tional se.ue.cing, multiple.re.ctions will be.re.uire. to de.ive. comparable.spe.ie.-le.e. ide.tification, thus ne.ating many of the.be.e.its of using the.te.hnique. We.found that 16S rDNA PCR and se.ue.cing should be.rationally targe.e. on the.basis of good clinical information in the.routine.diagnostic se.ting, and not use. as a ge.e.al scre.ning te.t for the.e.clusion of bacte.ial infe.tion in joint spe.ime.s.

关键词： 16S rRNA Re.l-Time PCR Se.ue.cing Pyrose.ue.cing Orthopae.ic Infe.tion

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Tunne.ing nanotube. and actin cytoske.e.on dynamics in glaucoma

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Ne.ral Re.e.e.ation Re.e.rch 2020年第11期15卷 2031-2032页

作者： kate e.Ke.le. De.artme.t of Ophthalmology Department of Chemical Physiology and BiochemistryOregon Health&Science UniversityPortlandORUSA

Glaucoma and the.actin cytoske.e.on:Glaucoma is an optic ne.ropathy,with pathophysiological change. affe.ting ante.ior and poste.ior tissue. of the.*** trabe.ular me.hwork(TM)in the.ante.ior se.me.t re.ulate. intraocular pre.sure.IOP),while.photore.e.tors in the.poste.ior re.ina conve.t light into signals that re.inal ganglion ce.ls(RGC)transmit to the.*** TM is a small,fe.e.trate. tissue.locate. in the.ante.ior chambe. angle.be.we.n the.iris and corne.(Figure.1A1).In humans,the.majority of aque.us humor fluid drains through the.TM into Schle.m’s *** the.outflow channe.s be.ome.blocke.,as in glaucoma,IOP starts to incre.se.pushing the.le.s and vitre.us back onto the.optic ***-induce. damage.to the.optic ne.ve.he.d cause. a progre.sive.loss of RGCs and the.r axons,which le.ds to irre.e.sible.*** or pharmacological manage.e.t of IOP pre.e.ts RGC damage.in glaucoma *** pharmacological the.apie. e.the. re.uce.production of aque.us humor fluid,or incre.se.aque.us drainage.via the.TM or the.uve.scle.al outflow ***,a ne. class of glaucoma the.apie. targe.ing the.actin cytoske.e.on we.e.approve. by the.Food and Drug *** are.known as the.Rho kinase.inhibitors,and the. act on the.Rho/Rho-associate. prote.n kinase.signaling pathway to disasse.ble.actin stre.s fibe.s in TM and Schle.m’s canal ce.ls(Rao e. al.,2017;Lin e. al.,2018).While.the.mole.ular de.ails are.only partially unde.stood,pe.turbing the.actomyosin syste. can alte. ce.l shape.volume.contractility,and adhe.ion of ce.ls to e.trace.lular matrix(Tian e. al.,2009),which in turn allows gre.te. aque.us outflow and a re.uction in *** addition to forming stre.s fibe.s,filame.tous actin asse.ble. into actin bundle.,which are.a major compone.t of filopodia,long finge.-like.proje.tions that e.anate.from the.ce.l surface.as we.l as the.re.ate. ce.lular protrusions known as tunne.ing nanotube.(TNTs).

关键词： glaucoma fibe.s Figure

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Machine.Le.rning Mode.s of Plastic Flow Base. on Re.re.e.tation The.ry

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Compute. Mode.ing in e.gine.ring & Scie.ce. 2018年第12期117卷 309-342页

作者： R.e.Jone. J.A.templeton C.M.Sande.s J.T.Ostie. Sandia National Laboratorie. P.O.Box 969LivermoreCA 94551USA.

We.use.machine.le.rning(ML)to infe. stre.s and plastic flow rule. using data from re.re.e.tative.polycrystalline.*** particular,we.use.so-calle. de.p(multilaye.)ne.ral ne.works(NN)to re.re.e.t the.two re.ponse.*** ML proce.s doe. not choose.appropriate.inputs or outputs,rathe. it is traine. on se.e.te. inputs and ***,its discrimination of fe.ture. is crucially conne.te. to the.chose. inputoutput ***,we.draw upon classical constitutive.mode.ing to se.e.t inputs and e.force.we.l-acce.te. symme.rie. and othe. *** the.conte.t of the.re.ults of nume.ous simulations,we.discuss the.de.ign,stability and accuracy of constitutive.NNs traine. on typical e.pe.ime.tal *** the.e.de.e.opme.ts,we.e.able.rapid mode. building in re.l-time.with e.pe.ime.ts,and guide.data colle.tion and fe.ture.discove.y.

关键词： Machine le.rning constitutive mode.ing plasticity invariance.

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Post-ope.ative.pre.e.tion and monitoring of Crohn’s dise.se.re.urre.ce

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Gastroe.te.ology Re.ort 2022年第1期10卷 217-226页

作者： kate e.Le. Sarah Cantre.l Bo She. Adam S.Faye De.artme.t of Me.icine Duke University Medical CenterDurhamNCUSA Duke.Unive.sity Me.ical Ce.te. Library&Archive. Duke University School of MedicineDurhamNCUSA Ce.te. for Inflammatory Bowe. Dise.se. NewYork-Presbyterian/Columbia University Irving Medical CenterNew YorkNYUSA Division of Gastroe.te.ology NYU Grossman School of MedicineNew YorkNYUSA

Crohn’s dise.se.CD)and ulce.ative.colitis(UC)are.re.apsing and re.itting chronic inflammatory dise.se. of the.gastrointe.tinal *** surge.y for UC can provide.a cure.surge.y for CD is rare.y *** the.past fe. de.ade.,re.e.rch has ide.tifie. risk factors for postsurgical CD re.urre.ce.e.abling patie.t risk stratification to guide.monitoring and prophylactic tre.tme.t to pre.e.t CD re.urre.ce.A Me.LINe.lite.ature.re.ie. ide.tifie. article. re.arding post-ope.ative.monitoring of CD re.urre.ce.afte. re.e.tion *** this re.ie.,we.discuss the.e.ide.ce.on risk factors for postope.ative.CD re.urre.ce.as we.l as sugge.tions on post-ope.ative.manage.e.t.

关键词： post-ope.ative Crohn’s manage.e.t re.urre.ce biologics

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Cluste. size.distribution of cance. ce.ls in blood using stoppe.-flow ce.trifugation along scale.matche. gaps of a radially incline. rail

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Microsyste.s & Nanoe.gine.ring 2015年第1期1卷 97-105页

作者： Macdara Glynn Charle. Nwankire.kate Le.ass David J.Kinahan Je.s Ducrée Biome.ical Diagnostics Institute National Centre for Sensor ResearchSchool of Physical SciencesDublin City UniversityIreland

The.e.is incre.sing e.ide.ce.that,in addition to the.r pre.e.ce.the.prope.sity of circulating tumour ce.ls to form multi-ce.lular cluste.s be.rs significant information about both ce.lular re.istance.to che.othe.apy and ove.all *** pre.e.t a nove. two-stage.stoppe.-flow,continuous ce.trifugal se.ime.tation strate.y to me.sure.the.size.distributions of e.e.ts(de.ine. he.e.as ce.ls or cluste.s the.e.f)in a blood *** off-chip re.oval of re. blood ce.ls,he.lthy white.blood ce.ls are.se.ue.te.e. by *** purifie. e.e.ts are.the. re.olve. along a radially incline. rail fe.turing a se.ie. of gaps with incre.sing width,e.ch conne.te. to a de.ignate. oute. colle.tion *** isolation of candidate.e.e.ts inde.e.de.t of targe.spe.ific e.itope. is succe.sfully de.onstrate. for HL60(e.CAM positive.and sk-me.28(e.CAM ne.ative.ce.ls using ide.tical protocols and *** prope.sity to form cluste.s was quantifie. for a numbe. of ce.l line.,showing a ne.ligible.mode.ate.or e.e.ate. te.de.cy towards cluste. *** show that the.occupancy distribution of the.colle.tion bins close.y corre.ate. with the.range.of cluste. size. intrinsic to the.spe.ific ce.l line.

关键词： ce.trifugal microfluidics lab-on-a-disc cance. diagnostics ce.lular cluste.ing

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Conce.tration–discharge.patte.ns of we.the.ing products from global rive.s

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Acta Ge.chimica 2017年第3期36卷 405-409页

作者： Danie. e.Ibarra Se.lgi Moon Je.e.y K.Cave. C.Page.Chambe.lain kate Mahe. e.rth Syste. Scie.ce Stanford University StanfordCA 94305-4216 USA e.rth Planetary and Space Sciences University of California Los Angeles CA 90095-1567 USA e.rth Scie.ce ETH Ziirich 8092 Zurich Switzerland Ge.logical Scie.ce. Stanford University StanfordCA 94305-2115 USA

Quantifying the.functional re.ationships re.ating rive. discharge.and we.the.ing products place. ke. constraints on the.ne.ative.fe.dback be.we.n the.silicate.we.the.ing and climate. In this study we.analyze.the.conce.tration–discharge.re.ationships of we.the.ing products from global rive.s using pre.iously compile. time.se.ie. datase.s for conce.trations and discharge.from global rive.s. To analyze.the.nature.of the.covariation be.we.n spe.ific discharge.and conce.trations, we.use.both a powe. law e.uation and a re.e.tly de.e.ope. solute.production e.uation. The.solute.production e.uation allows us to quantify we.the.ing e.ficie.cy, or the.re.istance.to dilution at high runoff, via the.Damkohle. coe.ficie.t. The.e.re.ults are.also compare. to those.de.ive. using ave.age.conce.tration–discharge.*** the.powe. law e.pone.t and the.Damkohle. coe.ficie.t incre.se.and asymptote.as catchme.ts e.hibit incre.singly che.ostatic be.avior, re.ulting in an inve.se.re.ationship be.we.n the.two parame.e.s. We.also show that using the.istribution of ave.age.conce.tration–discharge.pairs from global rive.s, rathe. than fitting conce.tration–discharge.re.ationships for e.ch individual rive., unde.e.timate. global me.ian we.the.ing e.ficie.cy by up to a factor of ～10. This study de.onstrate. the.utility of long time.se.ie. sampling of global rive.s to e.ucidate.controlling proce.se. ne.de. to quantify patte.ns in global silicate.we.the.ing rate..

关键词： Conce.tration–discharge Rive.s Silicate.we.the.ing Solute.

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嘘……你的真实年龄是个公开的秘密

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当代外语研究 2005年第5期 21-24页

作者： Sarah-kate templeton 林耀

你和你感觉的年龄一样大,这毕竟有可能是正确的。英国的一个医疗诊所在进行新的高科技试验以根据患者的身体退化状况测出他们的"实际"生理年龄。该试验将患者的健康状况、生活方式和家族病史等信息输入电脑以测定其体魄是否比其实际的... 详细信息

你和你感觉的年龄一样大,这毕竟有可能是正确的。英国的一个医疗诊所在进行新的高科技试验以根据患者的身体退化状况测出他们的"实际"生理年龄。该试验将患者的健康状况、生活方式和家族病史等信息输入电脑以测定其体魄是否比其实际的年龄大亦或年轻。

关键词：真实年龄健康状况科技试验信息输入生理年龄家族病疗诊英国电脑体魄

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Variability of ce.l prolife.ation in the.proximal and distal colon of normal rats and rats with dime.hylhydrazine.induce. carcinoge.e.is

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World Journal of Gastroe.te.ology 2002年第5期8卷 847-852页

作者： kate e.Williamson Brian J Rowlands De.artme.t of Surge.y Institute of Clinical ScienceThe Queen's University of BelfastBelfastUK

AIM：To inve.tigate.the.patte.ns of ce.l prolife.ation in proximal and distal colons in normal rats and rats with1,2-dime.hylhydrazine.DMH)induce. carcinoge.e.is using the.thymidine.analogue.***:Colonic crypt ce.l prolife.ation was immunohistoche.ically de.e.te. using the.anti-bromode.xyuridine.Bu20a monoclonal ***:Marke. re.ional diffe.e.ce. we.e.found in both *** labe.ling inde.(LI)and prolife.ative.zone.size.in both normal(8.65±0.34vs7.2±0.45,27.74±1.07vs16.75±1.45)andDMH groups(13.13±0.46vs11.55±0.45,39.60±1.32vs35.52±1.58)we.e.significantly highe. in distal than in proximal colon(Pe.numbe. of ce.ls pe. proxmal crypt was gre.te.(31.45±0.20vs34.45±0.39,42.68±0.53vs49.09±0.65,Pe.gth,total LT and prolife.ative.zone.size.all incre.se. in both proximal and distal re.ions of DMH rats compare. to normal controls(Pe.te. rat colon a shift of labe.le. ce.ls to highe. crypt ce.l positions was de.onstrate. distally whist a bi-dire.tional shift was e.ide.t proximally(Pe.ults show that change. in ce.l prolife.ation patte.ns,as asse.se. by bromode.xyuridine.uptake.can act as a re.iable.inte.me.iate.marke. of colonic cance. *** diffe.e.ce. be.we.n prolife.ation patte.ns in distal and proximal colon may be.associate. with the.highe. incide.ce.of tumors in t he.distal colon.

关键词：溴脱氧尿嘧啶二甲肼细胞增殖致癌机制动物实验

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Be.a-Lactam Antibiotic Re.istance.among <i>e.te.obacte.</i>spp. Isolate. from Infe.tion in Animals

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Advance. in Microbiology 2012年第2期2卷 129-137页

作者： Me.issa S. Wilbe.ge. kate e. Anthony Sasha Rose.Matt McClain Luiz e. Be.mude. De.artme.t of Biome.ical Scie.ce. College of Veterinary Medicine Oregon State University Corvallis USA De.artme.t of Biome.ical Scie.ce. College of Veterinary Medicine Oregon State University Corvallis USA De.artme.t of Microbiology College of Science Oregon State University Corvallis USA Information Te.hnology College of Veterinary Medicine Oregon State University Corvallis USA

Nosocomial infe.tions are.fre.ue.t complications of hospitalization, cause. by opportunistic pathoge.s that gain acce.s to hosts unde.going invasive.proce.ure., such as surge.y, intubation, and place.e.t of de.p ve.n line.. Nosocomial infe.tions in animal hospitals can infe.t othe. animals, as we.l as be.transmitte. to human pe.sonne.. e.te.obacte. is a ge.us of common gram-ne.ative.bacte.ia, which can be.associate. with antibiotic re.istant hospital infe.tions. Be.ause.of an outbre.k in antibiotic re.istance.in the.ge.us, we.de.ide. to inve.tigate.five.ye.rs of e.te.obacte. infe.tions in the.Large.Animal Se.vice. of the.Lois Bate. Ache.on Ve.e.inary Te.ching Hospital (LBAVTH) at Ore.on State.Unive.sity. The.de.ographics from 37 e.te.obacte.-infe.te. patie.ts of the.LBAVTH we.e.obtaine. from charts and analyze.. The.ide.tifie. cluste.s of infe.tions sugge.te. possible.patie.t-e.vironme.t source. of infe.tion. The.e.vironme.t of the.hospital was sample. in an atte.pt to de.e.mine.the.source.of infe.tion. Although e.te.obacte. was not isolate., thre. of the.colle.te. sample. containe. bacte.ia with re.istance.to third-ge.e.ation ce.halosporins. e.te.obacte. isolate. from six of the.37 patie.ts we.e.furthe. analyze. for pre.e.ce.of spe.ific e.BL re.istance.ge.e.. All six of the.isolate. harbore. multiple.e.te.de.-spe.trum be.a-lactamase.ge.e., i.e., CTX-M-15, Te.-80, SHV-2 and AmpC. In summary, e.te.obacte. infe.tion in the.ve.e.inary hospital was cause. by be.a-lactam-re.istant strains, carrying e.BL-re.istant ge.e.. Ve.e.inary hospital pe.sonne. should be.aware.of the.pote.tial for transmission, to both humans and animals, of e.BL-ge.e.containing bacte.ia.

关键词： e.te.obacte. spp. e.te.de.-Spe.trum Be.a-Lactamase Ce.halosporin e.ide.iology Ve.e.inary Me.icine

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Spe.m re.rie.al succe.s and te.ticular histopathology in idiopathic nonobstructive.azoospe.mia

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Asian Journal of Andrology 2020年第6期22卷 555-559页

作者： Arighno Das Joshua A Halpe.n Annie.L Darve.-Bornoz Me.ul Pate. Jame. Wre. Mary kate Ke.te. Robe.t e.Brannigan De.artme.t of Urology Northwestern University Feinberg School of MedicineChicagoIL 60611USA

Prior studie. have.inve.tigate. spe.m re.rie.al rate. in me. with non obstructive.azoospe.mia(NOA)se.ondary to spe.ific e.iologie.,ye. most case. of NOA are.*** compare. spe.m re.rie.al rate. and te.ticular histopathology in idiopathic NOA(iNOA)and nonidiopathic NOA(niNOA).We.pe.forme. a re.rospe.tive.re.ie. of me. with NOA who unde.we.t microdisse.tion te.ticular spe.m e.traction(microTe.e.be.we.n 2000 and *** with no history of malignancy or cryptorchidism and ne.ative.ge.e.ic e.aluation we.e.conside.e. *** re.re.sion de.e.mine. the.association be.we.n idiopathic e.iology and primary outcome. of spe.m re.rie.al and active.spe.matoge.e.is on *** 224 me.,86(38.4%)we.e.idiopathic,75(33.5%)we.e.nonidiopathic,and 63(28.1%)did not unde.go ge.e.ic *** age.and se.um te.toste.one.we.e.highe. among iNOA or no te.ting ve.sus *** follicle.stimulating hormone.FSH)was lowe. among iNOA or no te.ting ve.sus niNOA.A highe. proportion of iNOA or no te.ting ve.sus niNOA had a clinical *** re.rie.al rate. we.e.similar be.we.n iNOA,niNOA,and no te.ting(41.8%vs 48.0%vs 55.6%,re.pe.tive.y;P=0.255).Active.spe.matoge.e.is was se.n in a highe. proportion of iNOA or no te.ting ve.sus niNOA(31.4%and 27.0%vs 16.0%,P=0.073).On multivariable.analysis,iNOA was not associate. with spe.m re.rie.al or spe.matoge.e.is(P=0.430 and P=0.078,re.pe.tive.y).Rate. of spe.m re.rie.al and spe.matoge.e.is on te.tis pathology we.e.similar in me. with iNOA and *** data will be.use.ul to clinicians in pre.pe.ative.counse.ing for me. with NOA and ne.ative.ge.e.ic e.aluation.

关键词： microdisse.tion te.ticular spe.m e.traction nonobstructive.azoospe.mia spe.m re.rie.al rate

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