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Residual feed intake phenotype and gender affect the expression of key genes of the lipogenesis pathway in subcutaneous adipose tissue of beef cattle

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Journal of Animal Science and Biotechnology 2019年第1期10卷 208-217页

作者： Clare McKenna Richard K.Porter kate a.keogh Sinead M.Waters Mark McGee David A.Kenny Animal and Bioscience Research Department Teagasc Grange School of Biochemistry & Immunology Trinity College Dublin

Background: Feed accounts for up to 75% of costs in beef production systems,thus any improvement in feed efficiency(FE) will benefit the profitability of this *** feed intake(RFI) is a measure of FE that is independent of level of *** tissue(AT) is a major endocrine organ and the primary metabolic energy *** modulates a variety of processes related to FE such as lipid metabolism and glucose homeostasis and thus measures of inter-animal variation in adiposity are frequently included in the calculation of the RFI *** aim of this study was to determine the effect of phenotypic RFI status and gender on the expression of key candidate genes related to processes involved in energy metabolism within *** matter intake(DMI) and average daily gain(ADG) were measured over a period of 70 d for 52 purebred Simmental heifers(n = 24) and bulls(n = 28) with an initial BW±SD of 372±39.6 kg and 387±50.6 kg,*** feed intake was calculated and animals were ranked within gender by RFI into high(inefficient; n = 9 heifers and n = 8 bulls)and low(efficient; n = 9 heifers and n = 8 bulls) ***: Average daily gain ±SD and daily DMI ±SD for heifers and bul s were 1.2±0.4 kg and 9.1±0.5 kg,and 1.8±0.3 kg and 9.5±1 kg *** RFI heifers and bulls consumed 10% and 15% more(P < 0.05) than their low RFI counterparts,*** had a higher expression of all genes measured than bulls(P < 0.05).A gender × RFI interaction was detected for HMGCS2(P < 0.05) in which high RFI bulls tended to have lower expression of HMGCS2 than low RFI bulls(P < 0.1),whereas high RFI heifers had higher expression than low RFI heifers(P < 0.05) and high RFI bulls(P < 0.05).SLC2 A4 expression was consistently higher in subcutaneous AT of low RFI animals across ***: The findings of this study indicate that low RFI cattle exhibit upregulation of the molecular mechanisms governing glucose metabolism in adipose tiss

关键词： Adipose Cattle RFI SLC2A4

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Effect of genotype on duodenal expression of nutrient transporter genes in dairy cows

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Journal of Animal Science and Biotechnology 2014年第2期5卷 145-153页

作者： Sinéad M Waters kate keogh Frank Buckley David A Kenny Teagasc Animal and Bioscience Research Department Animal and Grassland Research and Innovation CentreGrangeDunsanyCo.MeathIreland Teagasc Animal and Bioscience Research Department Animal and Grassland Research and Innovation Centre MooreparkFermoyCo CorkIreland

Background： Studies have shown clear differences between dairy breeds in their feed intake and production efficiencies. The duodenum is critical in the coordination of digestion and absorption of nutrients. This study examined gene transcript abundance of important classes of nutrient transporters in the duodenum of non lactating dairy cows of different feed efficiency potential, namely Holstein-Friesian （HF）, Jersey （JE） and their F1 hybrid. Duodenal epithelial tissue was collected at slaughter and stored at -80℃. Total RNA was extracted from tissue and reverse transcribed to generate cDNA. Gene expression of the following transporters, namely nucleoside amino acid; sugar; mineral; and lipid transporters was measured using quantitative real-time RT-PCR. Data were statistically analysed using mixed models ANOVA in SAS. Orthogonal contrasts were used to test for potential heterotic effects and spearman correlation coefficients calculated to determine potential associations amongst gen, expression values and production efficiency variables. Results： While there were no direct effects of genotype on expression values for any of the genes examined, there was evidence for a heterotic effect （P 〈 0.05） on ABCGS, in the form of increased expression in the F1 genotype compared to either of the two parent breeds. Additionally, a tendency for increased expression of the amino acid transporters, SLC3A1 （P= 0.072）, SLC3A2 （P= 0.081） and SLC6A 14 （P= 0.072） was also evident in the F1 genotype. A negative （P 〈 0.05） association was identified between the expression of the glucose transporter gene SLCSA1 and total lactational milk solids yield, corrected for body weight. Positive correlations （P 〈 0.05） were also observed between the expression values of genes involved in common transporter roles. Conclusion： This study suggests that differences in the expression of sterol and amino acid transporters in the duodenum could contribute towards the documented differences in feed effic

关键词： Bovine Duodenum Gene expression Nutrient transporters

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Examination of the molecular control of ruminal epithelial function in response to dietary restriction and subsequent compensatory growth in cattle

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Journal of Animal Science and Biotechnology 2017年第1期8卷 107-118页

作者： Emma O＇Shea Sinead M. Waters kate keogh Alan K. Kelly David A. Kenny School of Agriculture and Food Science University College DublinBelfield Dublin 4Ireland Animal and Bioscience Research Department Animal & Grassland Research and Innovation Centre Teagasc Grange Dunsany Co Meath Ireland UCD Earth Institute University College Dublin Belfield Dublin 4 Ireland

Background： The objective of this study was to investigate the effect of dietary restriction and subsequent compensatory growth on the relative expression of genes involved in volatile fatty acid transport, metabolism and cell proliferation in ruminal epithelial tissue of beef cattle. Sixty Holstein Friesian bulls（mean liveweight 370 ± 35 kg;mean age 479 ± 15 d） were assigned to one of two groups：（i） restricted feed allowance（RES; n = 30） for 125 d（Period 1） followed by ad libitum access to feed for 55 d（Period 2） or（ii） ad libitum access to feed throughout（ADLIB; n = 30）. Target growth rate for RES was 0.6 kg/d during Period 1. At the end of each dietary period, 15 animals from each treatment group were slaughtered and ruminal epithelial tissue and liquid digesta harvested from the ventral sac of the rumen. Real-time q PCR was used to quantify m RNA transcripts of 26 genes associated with ruminal epithelial function. Volatile fatty acid analysis of rumen fluid from individual animals was conducted using gas ***： Diet × period interactions were evident for genes involved in ketogenesis（BDH2, P = 0.017）, pyruvate metabolism（LDHa, P = 0.048; PDHA1, P = 0.015） and cellular transport and structure（DSG1, P = 0.019; CACT, P = 0.027）. Ruminal concentrations of propionic acid（P = 0.018） and n-valeric acid（P = 0.029） were lower in RES animals, compared with ADLIB, throughout the experiment. There was also a strong tendency（P = 0.064）toward a diet × period interaction for n-butyric with higher concentrations in RES animals, compared with ADLIB, during Period ***： These data suggest that following nutrient restriction, the structural integrity of the rumen wall is compromised and there is upregulation of genes involved in the production of ketone bodies and breakdown of pyruvate for cellular energy. These results provide an insight into the potential molecular mechanisms regulating ruminal epithelial absorptive metabolism and growth following nutri

关键词： Beef cattle Compensatory growth Feed efficiency Nutrient restriction Rumen epithelium

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