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Semen analysis standardization： is there any problem in Polish laboratories？

Asian Journal of Andrology 2013年第5期15卷 616-621页

作者： Renata Walczak-Jedrzejowska katarzyna marc hlewsks Elzbieta Oszukowska Eliza Filipiak Leszek Bergier Jolanta Slowikowska-Hilczer Division of Andrology Department of Andrology and Reproductive Endocrinology Medical University of Lodz 91-425 Lodz Poland Division of Reproductive Endocrinology Department of Andrology and Reproductive Endocrinology Medical University of Lodz 91-425 Lodz Poland Diagnostyka Company Ltd Medical Laboratories 31-513 Cracow Poland

The aim of the study was to determine the degree of compliance of Polish laboratories with World Health Organization （WHO） recommendations, with regard to semen analysis methodology. A survey requesting information about methods of semen analysis was distributed to employees of 55 laboratories. Respondents who had participated in external seminological workshops （31%） were termed certified respondents （CR）, the remaining （69%）--non-certified respondents （NCR）. Only one laboratory （6%） in the CR group and none in the NCR were compliant with WHO guidelines for methods and equipment used to evaluate seminal volume, sperm motility, concentration, vitality and morphology. Most problems were of volume measurement （weighing method was reported by 17% of CR and 10% of NCR） and staining method for sperm morphology （Papanicolau or Diff-Quik were found in 33% of CR and 23% of NCR）. A three- or four-point grading of sperm motility was used by the majority of respondents; however, 17% of CR and 37% of NCR did not use a laboratory counter to tally spermatozoa. Although a haemocytometer method was used by 80% of laboratories in each group, the improved Neubauer chamber was used only by 42% of CR and 19% of NCR. In each group, 24% of laboratories did not perform a vitality test. Procedural errors and the interchangeable utilization of two or even three methods to analyse a given parameter was observed in both groups. The results indicate a need for standardisation of the methods and continuous, unified training in semen analysis in Polish laboratories.

关键词： clinical laboratory compliance semen analysis World Health Organization

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