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Drosophila melanogaster as a model system of aluminum toxicity and aging

Insect Science 2014年第2期21卷 189-202页

作者： Ewelina Kijak Ezio Rosato katarzyna knapczyk Elzbieta Pyza Department of Cell Biology and Imaging Institute of Zoology Jagiellonian University Krakow Poland Department of Genetics University of Leicester Leicester LE1 7RH United Kingdom

The aim of this study was to investigate the toxic effects of aluminum （A1） on the model organism-Drosophila melanogaster. The study is especially concerned with the effects of aluminum on the fruit fly＇s development, life span, and circadian rhythm in rest and activity. Flies were exposed to aluminum in concentrations from 40 to 280 mg/kg in rearing media or the flies were raised on control medium. Moreover, the life span of insects exposed to aluminum containing 40, 120, or 240 mg/kg of A1 in the medium, only during their larval development, during the whole life cycle and only in their adult life was tested. To check if aluminum and aging cause changes in D. melanogaster behavior, the locomotor activity of flies at different ages was recorded. Results showed that aluminum is toxic in concentrations above 160 mg/kg in the rearing medium. Depending on A1 concentration and time of exposure, the life span of the flies was shortened. At intermediate concentrations （120 mg/kg）, however, A1 had a stimulating effect on males increasing their life span and level of locomotor activity. At higher concentration the aluminum exposure increased or decreased the level of locomotor activity ofD. melanogaster depending on age of flies. In addition, in the oldest insects reared on aluminum supplemented media and in mid-aged flies reared on the highest concentration of A1 the daily rhythm of activity was disrupted.

关键词： circadian rhythms development life-span locomotor activity rest/activity cycle survival

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Flutamide-induced alterations in transcriptional profiling of neonatal porcine ovaries

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Journal of Animal Science and Biotechnology 2019年第3期10卷 618-632页

作者： katarzyna knapczyk-Stwora Anna Nynca Renata E.Ciereszko Lukasz Paukszto Jan P.Jastrzebski Elzbieta Czaja Patrycja Witek Marek Koziorowski Maria Slomczynska Department of Endocrinology Institute of Zoology and Biomedical ResearchJagiellonian University in KrakowGronostajowa 9 Street30-387 KrakowPoland Laboratory of Molecular Diagnostics Faculty of Biology and BiotechnologyUniversity of Warmia and Mazury in OlsztynOlsztynPoland Department of Animal Anatomy and Physiology Faculty of Biology and BiotechnologyUniversity of Warmia and Mazury in OlsztynOlsztynPoland Department of Plant Physiology Genetics and BiotechnologyFaculty of Biology and BiotechnologyUniversity of Warmia and Mazury in OlsztynOlsztynPoland Department of Physiology and Reproduction of Animals University of RzeszowRzeszowPoland

Background: Androgens are involved in the regulation of ovarian development during fetal/neonatal *** chemicals displaying anti-androgenic activities may affect multiple signal transduction pathways by blocking endogenous androgen *** aim of the current study was to examine effects of the anti-androgen flutamide on the expression of coding transcripts and long non-coding RNAs(lncRNAs) in neonatal porcine *** employing RNA-Seq technology we aimed to extend our understanding of the role of androgens in neonatal folliculogenesis and examine the impact of the anti-androgen flutamide on ovarian ***: Piglets were subcutaneously injected with flutamide(50 mg/kg BW) or corn oil(controls) between postnatal days 1 and 10(n = 3/group).Ovaries were excised from the 11-day-old piglets and total cellular RNAs were isolated and ***: Flutamide-treated piglet ovaries showed 280 differentially expressed genes(DEGs;P-adjusted < 0.05 and log2 fold change ≥1.0) and 98 differentially expressed lncRNAs(DELs;P-adjusted < 0.05 and log2 FC ≥ 1.0).The DEGs were assigned to GO term,covering biological processes,molecular functions and cellular components,which linked the DEGs to functions associated with cellular transport,cell divisions and *** addition,STRING software demonstrated strongest interactions between genes related to cell *** between DEGs and DELs were also found,revealing that a majority of the genes targeted by the flutamide-affected lncRNAs were associated with intracellular transport and cell ***: Our results suggest that neonatal exposure of pigs to flutamide alters the expression of genes involved in ovarian cell proliferation,ovarian steroidogenesis and oocyte fertilization,which in turn may affect female reproduction in adult life.

关键词： Flutamide LncRNA Ovary Pig RNA-Seq Transcriptome

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雌激素受体α、β在妊娠猪子宫中的表达

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中国畜牧兽医 2009年第10期36卷 192-192页

作者： K knapczyk-Stwora 晋大鹏

众所周知,子宫是一个内分泌、旁分泌和自分泌的分子作用靶器官,类固醇激素对其具有特殊的重要性。本试验通过RT-PCR、Western bloting和免疫组化技术对怀孕猪整个妊娠期(交配后10、18、32、50、71、90 d)的雌激素受体(ERα和ERβ)mRNA... 详细信息

众所周知,子宫是一个内分泌、旁分泌和自分泌的分子作用靶器官,类固醇激素对其具有特殊的重要性。本试验通过RT-PCR、Western bloting和免疫组化技术对怀孕猪整个妊娠期(交配后10、18、32、50、71、90 d)的雌激素受体(ERα和ERβ)mRNA和蛋白质进行了定位。研究结果首次发现,猪的整个妊娠周期中都有雌激素受体蛋白存在。ERα存在于怀孕猪子宫的腔上皮(LE)、腺上皮(GE)细胞和子宫肌层的肌细胞中,而且在妊娠的不同阶段染色的强度并不相同;在怀孕猪妊娠周期内LE和GE细胞中均可检测到ERβ,但子宫肌层上仅只有少许细胞中存在ERβ,并且染色结果显示弱免疫反应。Western bloting分析结果表明,试验期内都能检测到ERα和ERβ蛋白。RT-PCR结果显示,在妊娠期的每一阶段都有ERα和ERβ的mRNA表达。以上结果表明,相对ERβ而言,妊娠猪子宫中ERα含量更丰富,且占主导地位;怀孕猪整个妊娠周期内都能发现ERα和ERβ,并且可能对雌激素细胞的增值和分化起直接作用。

关键词：雌激素受体α 妊娠猪子宫雌激素受体β

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