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Expression profiling of transgenes(Cry1Ac and Cry2A) in cotton genotypes under different genetic backgrounds

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Journal of Integrative Agriculture 2022年第10期21卷 2818-2832页

作者： kashif NOOR Hafiza Masooma naseer CHEEMA Asif Ali KHAN Rao Sohail Ahmad KHAN Department of Plant Breeding and Genetics University of AgricultureFaisalabad 38040Pakistan Institute of Plant Breeding and Biotechnology Muhammad Nawaz Shareef University of AgricultureMultan 60000Pakistan Center of Agricultural Biochemistry and Biotechnology University of AgricultureFaisalabad 38040Pakistan

Transgenic cotton carrying the CrylAc gene has revolutionized insect pest control since its adoption,although the development of resistance in insect pests has reduced its *** 10 years of cultivating Bacillus thuringiensis(Bt)cotton with a single Cry1 Ac gene,growers are on the verge of adopting Bt cotton that carries the double gene(Cry1 Ac+Cry2 A)due to its better effectiveness against insect ***,the current study was designed to evaluate the role of each gene in the effectiveness of Bt cotton carrying the double *** expression levels of the Cry1 Ac and Cry2 A genes were evaluated in the leaves of 10 genotypes(2 parents and 8 Fhybrids)at 30 days after sowing(DAS),while samples of leaves,bolls and flowers were taken from the upper and lower canopies at 70 and 110 *** Fhybrids were developed through reciprocal crosses between two Bt(CKC-1,CKC-2)and two non-Bt(MNH-786,FH-942)*** differential expression of transgenes was evaluated through Enzyme Linked Immuno-Sorbent Assay(ELISA).The results showed that the MNH786 xCKC-1 hybrid had the highest concentrations of Cry1 Ac gene at30 DAS(3.08μg g^(-1))and 110 DAS(1.01μg g^(-1))in *** contrast,the CKC-2 xMNH-786 hybrid showed the lowest concentrations of Cry1 Ac gene at 30 DAS(2.30μg g^(-1))and 110 DAS(0.86μg g^(-1)).The Fhybrid FH-942×CKC-2 showed the highest concentrations of Cry2 A gene at 30 DAS(8.39μg g^(-1))and 110 DAS(7.74μg g^(-1))in leaves,while the CKC-1 xMNH-786 hybrid expressed the lowest concentrations of Cry2 A gene at 30 DAS(7.10μg g^(-1))and 110 DAS(8.31μg g^(-1)).A comparison between the two stages of plant growth showed that leaves had the highest concentrations at 30 DAS,whereas the lowest concentrations were observed at 110 DAS for both genes in *** the expression pattern was compared between various plant parts in genotype CKC-2,it was found that leaves had higher concentrations of Cry1 Ac(3.12μg g^(-1))and Cry2 A(8.31μg g^(-1))at 70 DAS,followed by bolls

关键词： transgenic cotton breeding transgene expression double gene insect resistance

Hemodynamic Response Detection Using Integrated EEG-fNIRS-VPA for BCI

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Computers, Materials & Continua 2022年第1期70卷 535-555页

作者： Arshia Arif M.Jawad Khan kashif Javed Hasan Sajid Saddaf Rubab Noman naseer Talha Irfan Khan National University of Sciences and Technology(NUST) IslamabadPakistan Intelligent Robotics Lab National Center of Artificial IntelligenceNational University of Sciences and Technology(NUST)IslamabadPakistan Department of Mechatronics Engineering Air UniversityIslamabadPakistan Institute of Space Technology IslamabadPakistan

For BCI systems,it is important to have an accurate and less complex architecture to control a device with enhanced *** this paper,a novel methodology for more accurate detection of the hemodynamic response has been developed using a multimodal brain-computer interface(BCI).An integrated classifier has been developed for achieving better classification accuracy using two *** integrated EEG-fNIRS-based vector-phase analysis(VPA)has been *** open-source dataset collected at the TechnischeUniversit鋞Berlin,including simultaneous electroencephalography(EEG)and functional near-infrared spectroscopy(fNIRS)signals of 26 healthy participants during n-back tests,has been used for this *** and physiological noise removal has been done using preprocessing techniques followed by individually detecting activity in both *** resting state threshold circle,VPA has been used to detect a hemodynamic response in fNIRS signals,whereas phase plots for EEG signals have been constructed using Hilbert Transform to detect the activity in each *** threshold circles are drawn in the vector plane,where each circle is drawn after task completion in each trial of EEG ***,both processes are integrated into one vector-phase plot to get combined detection of hemodynamic response for *** of this study illustrate that the combined EEG-fNIRS VPA yields considerably higher average classification accuracy,that is 91.35%,as compared to other classifiers such as support vector machine(SVM),convolutional neural networks(CNN),deep neural networks(DNN)and VPA(with dual-threshold circles)with classification accuracies 82%,89%,87%and 86%*** of this research demonstrate that improved classification performance can be feasibly achieved using multimodal VPA for EEG-fNIRS hybrid data.

关键词： EEG-fNIRS hybrid BCI vector-phase analysis hemodynamic response detection

Differential elastic scattering cross sections of protons from Al in 2.4–4.8 MeV energy range

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Nuclear Science and Techniques 2016年第2期27卷 97-100页

作者： kashif Shahzad Faisal J. qureshi Javeria Taj Ali Awais Javaid Hussain Waheed Akram Shehla Honey Ishaq Ahmad Maaza Malik National Center for Physics Quaid-i-Azam UniversityIslamabad 44000 Pakistan UNESCO-UNISA Africa Chair in Nanosciences/ Nanotechnology College of Graduate Studies University of South Africa Muckleneuk Ridge PO Box 392 Pretoria South Africa Nanosciences African Network (NANOAFNET) iThemba LABS-National Research Foundation 1 Old Faure Road Somerset West 7129 PO Box 722 Somerset West Western Cape Province South Africa

Measurement of differential elastic cross section of protons from aluminum was taken at 165° degree in the2.4–4.8 Me V energy range. The results and measured energy resonances were compared with reported *** data wi... 详细信息

关键词：能量范围微分截面弹性散射质子电子铝能量共振后向散射

Effects of droplet size and spray interval on root-to-shoot ratio,photosynthesis efficiency,and nutritional quality of aeroponically grown butter head lettuce

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International Journal of Agricultural and Biological Engineering 2022年第1期15卷 79-88页

作者： Mazhar Hussain Tunio Jianmin Gao Waqar Ahmed qureshi Sher Ali Sheikh Jiedong Chen Farman Ali Chandio Imran Ali Lakhiar kashif Ali Solangi School of Agricultural Engineering Jiangsu UniversityZhenjiang 212013JiangsuChina

The proper selection of the atomizer(droplet size)and nutrient solution spray interval is one of the most important factors to be investigated in aeroponics system for sustainable *** aim of this study was to research the effects of four aeroponics atomizing nozzles(one air-assisted;A_(1),two air-less;A_(2) and A_(3),and one ultrasonic fogger;A_(4))with droplet sizes of 11.24μm,26.35μm,17.38μm,and 4.89μm,respectively,four spray intervals(15 min(I_(1)),30 min(I_(2)),45 min(I_(3))and 60 min(I_(4)))at a 5 min of constant spray time by atomizing the Hoagland’s nutrient solution on growth,root-to-shoot ratio,photosynthesis characteristics,pigments,and nutritional quality of the aeroponically grown *** experimental results demonstrated that in A_(1) atomizer and I_(2) interval,the growth,photosynthesis efficiency,chlorophyll,carotenoids,and nutritional values of the lettuce were significantly higher compared to that grown in A_(2) and A_(3) atomizers at all spray *** shoot developments were more constrained than root,prominent to the alteration of root-to-shoot ratio(fresh and dry)in the influence of different droplet sizes and spray ***,the plants did not grow well in A_(4) atomizer associated with proposed spray *** results disclosed that there was an obvious interaction between droplet sizes(atomizers)and spray intervals for growth,the ratio of root to shoot,photosynthesis efficiency,pigments,and nutritional quality of the aeroponically grown *** research study increases the awareness of the proper droplet size(atomizer)and the regulation of nutrient solution spray interval for leafy vegetables grown in an aeroponics system.

关键词： aeroponics system atomizers droplet sizes photosynthesis efficiency chlorophyll contents quality of lettuce

Application of Seismic Attributes for Delineation of Channel Geometries and Analysis of Various Aspects in Terms of Lithological and Structural Perspectives of Lower Goru Formation, Pakistan

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International Journal of Geosciences 2014年第12期5卷 1490-1502页

作者： Tayyab Muhammad naseer Shazia Asim Mirza naseer Ahmad Farrukh Hussain Shahid Nadeem qureshi Department of Earth Sciences Quaid-I-Azam University Islamabad Pakistan China University of Geosciences Wuhan China School of Sciences Nusrat Jahan College Rabwah Pakistan Deewan Petroleum Limited Islamabad Pakistan COMSATS Institute of Information Technology (CIIT) Islamabad Pakistan

Miano area is one of the distinct major hydrocarbon producing fields of the Lower Indus basin. Lower Goru is the reservoir strait in this area. The aim of the present study is to exploit the channels reservoirs and other stratigraphic features in such a terrain where there is always a challenge for the geoscientist to search and exploit the channeled reservoirs. To resolve this issue we have utilized attribute analysis on high resolution 3D-seismic data for the detailed comparative studies for the channels. There are many astonishing features that are identified in the current study, which could not otherwise be easily enhanced with the help of 2D Seismic Data. Seismic attributes such as coherency, frequency (are also appropriate for lithological discrimination), which are sensitive to the channel edges are applied for the channel delineation and their geometrical analysis. Spectral decomposition techniques are also applied for the delineation of channels and to appropriately select the best band for channels identification. Three types of channel geometries are recognized: 1) highly sinuous channel;2) narrow-broad meandering belts;3) moderate to high sinuous channel. NW-SE, N-W trending faults can be helpful to compartmentalize the reservoir. Instantaneous and dominant frequency are more beneficial for further field development based on Gamma Ray logs from nearby drilled wells and dimensional perspectives analysis of the channel reservoir.

关键词： Seismic Attributes Spectral Decomposition Lower Goru Strait Miano Lower Indus Basin Channels

Towards a Remote Sensing and GIS-Based Technique to Study Population and Urban Growth: A Case Study of Multan

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Advances in Remote Sensing 2018年第3期7卷 245-258页

作者： Atif Ali Anam Khalid Muhammad Atif Butt Rashid Mehmood Syed Amer Mahmood Javed Sami Jahanzeb qureshi kashif Shafique Asdullah Khan Ghalib Rizwan Waheed Farhan Ali Rais Mukhtar Muhammad Azhar Department of Space Science University of the Punjab Lahore Pakistan

In recent decades, the migration rates of the large cities of Punjab have been risen up to a considerable level due to the lack of employment opportunities as well as lack of facilities in the rural areas of the province. It has caused an unprecedented and unplanned urbanization across the urban areas of the province. This study has been undertaken to perform fractal analysis about the sprawl in rapidly growing city. GIS and remote sensing data have been used in this study as an emerging technology which is cost effective as well as accurate at the same time. Landsat images have been taken for the study and the sprawl has been calculated with the analysis of the data of each decade for past more than 40 years. It has been observed that the built up area is 47.8 to 141.12 Sq. Km whereas the pattern of urban settlement has been classified as clustered and linear, following the roads network. The temporal population growth also seconded these results. The population growth rate and population density increase, are based on the pixel based extraction of the data from satellite imagery for the period of 2000 to 2014, which is taken as a decision support tool. In 2000, the population of the district increased from 2,071,694 (1981 census) to 2,939,907 and then in 2013, it became 4,384,191 at a rate to 2.93% upturn per annum. Moreover, the study also reveals the extent of the growth of other land uses as well which may be taken as a reference that in an agricultural country like Pakistan, the natural resources are being wasted (by urbanization of the fertile land). There must be some master planning to avoid such things in the other cities as well.

关键词： Urban Sprawl Population Growth Spatio-Temporal Remote Sensing Land Use Pakistan