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Isolation of prostrate turfgrass mutants via screening of dwarf phenotype and characterization of a perennial ryegrass prostrate mutant

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Horticulture Research 2016年第1期3卷 296-301页

作者： Junmei Chen Chandra Thammina Wei Li Hao Yu Huseyin Yer Rania El-Tanbouly Manon Marron Lorenzo Katin-Grazzini Yongqin Chen John Inguagiato Richard J.McAvoy karl guillard Xian Zhang Yi Li College of Landscape Architecture and Arts Northwest A&F UniversityYangling 712100China Department of Plant Science and Landscape Architecture University of ConnecticutStorrsCT 06269USA College of Life Sciences Hubei UniversityWuhan 430062China College of Horticulture Northwest A&F universityYangling 712100China

Prostrate turf varieties are desirable because of their increased low mowing tolerance,heat resistance,traffic resistance and ground coverage compared with upright *** breeding may provide a powerful tool to create prostrate varieties,but there are no simple,straightforward methods to screen for such *** of the molecular basis of the major‘green revolution’traits,dwarfism and semi-dwarfism,guided us to design a simple strategy for isolating dwarf mutants of perennial ryegrass(Lolium perenne L.).We have shown that gamma-ray-mediated dominant dwarf mutants can be easily screened for at the three-leaf *** 10%of dwarf mutant lines also displayed a prostrate phenotype at mature stages(>10 tillers).One prostrate line,Lowboy I,has been characterized in *** I had significantly shorter canopy,leaf blade and internode lengths compared with wild *** I also exhibited greater tolerance to low mowing stress than wild *** gibberellic acid(GA)restored Lowboy I to a wild-type phenotype,indicating that the dwarf and prostrate phenotypes were both due to GA *** further showed that phenotypes of Lowboy I were dominant and stably inherited through sexual *** turfgrass mutants are difficult to screen for because the phenotype is not observed at young seedling stages,therefore our method represents a simple strategy for easily isolating prostrate ***,Lowboy I may provide an outstanding germplasm for breeding novel prostrate perennial ryegrass cultivars.

关键词： dwarf breeding seedling

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