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Isolation of Pathogenic Gram.Negative Bacteria from.Urinary Tract Infected Patients

Open Journal of m.dical m.crobiology 2016年第2期6卷 59-65页

作者： m.hm.od K. Salih Nizar I. Alrabadi karkaz m. thalij Ali S. Hussien Departm.nt of Biology College of Education for Pure Sciences Tikrit University Tikrit Iraq Departm.nt of Food Science and Nutrition Faculty of Agriculture Jerash University Jerash Jordan Departm.nt of Biology Iraqi University Baghdad Iraq

This study investigated the susceptibility pattern of different bacteria isolated from.urinary tract infection to different antibiotics. 83 uropathogen bacteria were isolated from.300 urine sam.les taken from.patients attended to Tikrit Teaching Hospital from.m.rch, 2011 through February, 2012. The patients were m.les and fem.les aged between 4 days to 95 years. Bacteria obtained from.urine sam.les were cultured and tested for antim.crobial susceptibility to 16 kinds of antibiotics. Urine sam.les were cultured on different m.dia and incubated, thereafter bacteria were isolated and purified by streaking four tim.s on the sam. m.dia;isolates were identified depending on m.rphological, m.croscopic, and biochem.cal characteristics. The isolated strains of bacteria were tested for their susceptibility to som. antibiotics using disk diffusion m.thod. The antagonistic activity was evaluated by observing a clear zone of inhibition growth. The results showed that the bacterial species of Eschericia coli, Proteus m.rabilis, Klebsiella pneum.nia, Citrobacter diversus, Citrobacter freundii, Enterobacter aerogenes, Yersinia pestis, Pseudom.nas aeruginosa, Klebsiella oxytoca and Hafnia alvei were identified in 44 (53%), 18 (21.7%), 4 (4.8%), 4 (4.8%), 3 (3.6%), 3 (3.6%), 3 (3.6%), 2 (2.4%), 1 (1.2%) and 1 (1.2%), respectively, of the isolates. The results of antim.crobial susceptibility test showed that 83 (100%) isolates were resistant to Am.icillin, Rifam.icin and Erythrom.cin. 75 (90.3%) isolates were resistant to Cefotaxim., 67 (80.7%) isolates were resistant to Tobram.ci. 66 (79.5%), 65 (78.3%), 56 (67.4%) and 48 (57.8%) isolates showed susceptibility to Nalidixic acid, Tetracycline, Nitrofurantoin, Chloram.henicol, respectively. 45 (54.2%) isolates were resistant to Azithrom.cin, Norfloxacin and Ciprofloxacin. m.ropenem. Gentam.cin, Am.kacin, and Im.penem.show significant effect on 35 (42.1%), 32 (38.5%), 27 (32.5%) and 1 (1.2%) isolates, respectively. In conclusion, signif

关键词： Urinary Tract Infections Antibiotics Bacteria Antim.crobial Susceptibility Test

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