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Effect of epidermal growth factor on buffalo frozen spermatozoa biometry and metabolic activity

Asian pacific Journal of Reproduction 2017年第1期6卷 46-51页

作者： Mohamed MM Kandiel Ahmed RM El-Khawagah karima gh mahmoud Department of Theriogenology Faculty of Veterinary MedicineBenha UniversityEgypt Department of Animal Reproduction and Artificial Insemination National Research CentreDokkiGizaEgypt

Objective:To assess the effects of epidermal growth factor (EGF) on the Egyptian buffalo bull frozen semen, EGF was incorporated at 0 (control), 50, 100, 200 and 400 ng/mL of extender (Bioxcell?).Methods:Semen features, spermatozoa biometry, total liberated amounts of enzymes (aspartate transaminase, alanine aminotransferase, lactate dehydrogenase, acid phosphatase and alkaline phosphatase) and lipid peroxidation markers (thiobarbituric acid reactive substances, malondialdehyde, glutathione peroxidase, nitric oxide, catalase (CAT) and superoxide dismutase (SOD)) were determined in the spermatozoa-free extracellular extender. Results:Spermatozoa membrane integrity significantly (P<0.05) increased, but DNA integrity decreased with EGF 200 ng/mL. Spermatozoa head (dimensions, area and perimeter), but not shape, as well as acrosome and midpiece measures substantially differed with regard to EGF. Principle piece length and volume markedly decreased (at 100 and 200 ng/mL), while total tail/flagellum length increased (at 50 ng/mL) after EGF supplementation. EGF 50 ng/mL was associated with the decline of nitric oxide levels and catalase enzyme activity, but EGF 100 ng/mL significantly decreased the total liberated amounts of enzymes (aspartate transaminase, lactate dehydrogenase, acid phosphatase and alkaline phosphatase) as well as lipid peroxidation markers (thiobarbituric acid reactive substances and malondialdehyde).Conclusion:EGFin vitro supplementation would affect the semen characteristics of buffalo bull with 100 ng/mL counteracted the freezing mediated oxidative stress indicated with the lowest enzymes leakage and lipid peroxidation.

关键词： Buffalo Epidermal growth factor Lipid peroxidation Seminal enzymes Speriogram

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Genetic characterization of FSH beta-subunit gene and its association with buffalo fertility

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Asian pacific Journal of Reproduction 2017年第5期6卷 193-196页

作者： Ahmed S.A.Sosa karima gh M.mahmoud Mohamed M.M.Kandiel Hazem A.A.Eldebaky mahmoud F.Nawito mahmoud E.A.Abou El-Roos Department of Animal Reproduction&A.I National Research CentreDokkiTahrir Street12622 GizaEgypt Department of Theriogenology Faculty of Veterinary MedicineBenha UniversityKaliobiaEgypt

Objective:To study genetic variation in buffalo follicle stimulating hormone beta-subunit (FSHB) gene and its association with ***: In this experimental study, blood samples were collected by standard methods using EDTA anticoagulant and transrectal ultrasound examination was conducted on fertile (n=74) and infertile buffaloes with a history of anestrum (n=30) or repeat breeding (n=12). The genomic DNA was extracted for PCR followed by single strand conformation polymorphism analysis. DNA sequencing was performed for the determination of single nucleotide polymorphism ofFSHB ***:The study results showed that there was genetic polymorphism with two different single strand conformation polymorphism patterns, AA and AB. The former pattern was associated with fertility in Egyptian buffaloes. Pair wise alignment of the two patterns sequences revealed that FSHB pattern II (AB) has C nucleotide insertion as SNP at the site of 208 bp of sequenced ***:FSHB is polymorphic in the infertile Egyptian buffaloes, suggesting its practicability as a candidate marker for female fertility.

关键词： Buffalo FSH beta-subunit gene Nucleotide sequences SSCP analysis

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Effect of using ascorbic acid and cysteamine supplementation onin-vitrodevelopment of buffalo embryos

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Asian pacific Journal of Reproduction 2017年第2期6卷 85-88页

作者： Al-shimaa Al-H.H.El-Naby karima gh.M.mahmoud Gamal A.M.Sosa mahmoud E.A.Abouel-Roos Youssef F.Ahmed Department of Theriogenology Faculty of Veterinary MedicineBenha UniversityEgypt Department of Animal Reproduction and A.I. National Research CenterDokkiGizaEgypt

Objective:To improvein vitro embryo production in buffalo by supplementation of L-ascorbic acid during maturation and development (experiment 1) and combination with another antioxidant as cysteamine (experiment 2).Methods:Two experiments were performed, the first one aimed to evaluate the different concentrations (0, 25, 50, 100 μM) of L-ascorbic acid on embryo developmental rate of buffalo oocytes. The L-ascorbic acid was added to the maturation and culture media. In the second experiment, oocytes were cultured in media with two type of antioxidant (ascorbic acid + cysteamine) or ascorbic acid ***:There was a significant increase in cleavage rate at 25, 50 μM than 100 μM and control group. But, the blastocyst rate was higher at 50 μM ascorbic acid than other concentrations (0, 25, 100 μM). Supplementation of ascorbic acid and cysteamine to maturation and cultured media improved embryo development than ascorbic acid ***: Using of 50 μM L-ascorbic acid duringin vitro maturation and development improve the developmental competence of buffalo oocytes, this effect was increase with the presence of cysteamine.

关键词： Buffalo Oocytes L-ascorbic acid Cysteamine Embryo development

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Effect of buffalo bull breeds on developmental competence and vitrification ofin-vitro produced embryos

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Asian pacific Journal of Reproduction 2018年第6期7卷 270-273页

作者： Walid T.M.Soliman Al-Shimaa Al-H.H.El-Naby karima gh.M.mahmoud Ahmed R.M.El-Khawagah Mohamed M.M.Kandiel mahmoud E.A.Abouel-Roos Alaa E.Abdel-ghaffar AbdEl Salam I.El Azab Veterinary Department Egyptian Armed ForcesEgypt Department of Theriogenology Faculty of Veterinary MedicineBenha UniversityEgypt Department of Animal Reproduction and Artificial Insemination National Research CenterDokkiGizaEgypt

Objective:To assess effect of buffalo bull breed on the development and cryotolerence of the in vitro produced ***: Three types of frozen semen were adopted;Egyptian, Italian and cross-bred (Egyptian-Italian) breeds were used for in-vitro fertilization and vitrification of their embryos. Oocytes were collected from buffalo ovaries and maturedin vitrofor 24 h, then they were fertilized using the three semen breeds. The produced embryos of morula and blastocysts were vitrified using ethylene glycol and dimethyl sulfoxide then evaluated for their viability after ***: The cleavage and blastocysts rates significantly declined in oocytes fertilized by Egyptian (P<0.01) than in Italian (P<0.05) and crossbred (P<0.05) frozen semen. After embryo vitrification, there were no significant differences among the three breeds in the percentages of morphologically viable embryos evaluated directly after warming and at 24 h post-culture. Conclusions:Thein vitro fertilization response to frozen-thawed semen varies between breeds;however, the resistance of produced embryos to the damage effect of vitrification does not vary.

关键词： In vitro fertilization Buffalo breed Embryo production Vitrification

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