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A novel diagnostic gene region for distinguishing between two pest fruit flies:Bactrocera tryoni(froggatt)and Bactrocera neohumeralis(Hardy)(Diptera:Tephritidae)

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Insect Science 2024年第4期31卷 1285-1295页

作者： Melissa L.Starkie Elizabeth V.fowler Alexander M.Piper Xiaocheng Zhu Pauline Wyatt David Gopurenko Matt N.Krosch francesca Strutt karen f.armstrong Hamish Patrick Mark K.Schutze Mark J.Blacket Department of Agriculture and fisheries BiosecurityBrisbaneQueenslandAustralia Agriculture Victoria AgriBioBundooraVictoriaAustralia Department of Primary Industries Wagga Wagga Agricultural InstituteWagga WaggaNew South WalesAustralia forensic Services Group Queensland Police ServiceBrisbaneQueenslandAustralia Department of Pest-management and Conservation Lincoln UniversityLincolnNew Zealand Better Border Biosecurity(B3 B3nz.org.nz)LincolnNew Zealand

Bactrocera tryoni and Bactrocera neohumeralis are morphologically similar sibling pest fruit fly species that possess different biological attributes,geographic distributions,and host *** need to differentiate between the two species is critical for accurate pest status assessment,management,biosecurity,and maintenance of reference *** morphologically similar,adults may be separated based on subtle characters;however,some characters exhibit intraspecific variability,creating overlap between the two ***,there is currently no single molecular marker or rapid diagnostic assay that can reliably distinguish between *** and ***;therefore,ambiguous samples remain *** we report the first molecular marker that can consistently distinguish between *** and *** diagnostic region consists of two adjacent single nucleotide polymorphisms(SNPs)within the pangolin(pan)gene *** confirmed the genotypes of each species are consistent across their distributional range,then developed a tetra-primer amplification refractory mutation system(ARMS)PCR assay for rapid diagnosis of the *** assay utilizes four primers in multiplex,with two outer universal primers,and two internal primers:one designed to target two adjacent SNPs(AA)present in *** and the other targeting adjacent SNPs present in ***(GG).The assay accurately discriminates between the two species,but their SNP genotypes are shared with other nontarget tephritid fruit fly ***,this assay is most suited to adult diagnostics where species confirmation is necessary in determining ambiguous surveillance trap catches;maintaining pure colony lines;and in Sterile Insect Technique management responses.

关键词： ARMS-PCR colony molecular diagnostics pangolin tetra-primer

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Prospective cohort study on facial profile changes in infants with Robin sequence and healthy controls

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World Journal of Pediatrics 2024年第6期20卷 581-589页

作者： Cornelia Wiechers Julian Sowula karen Kreutzer Christoph E.Schwarz Christina Weismann Michael Krimmel Christian f.Poets Bernd Koos Department of Neonatology Tuebingen University HospitalCalwerstrasse 772076 TuebingenGermany Center for Cleft Lip Palate and Craniofacial MalformationsTuebingen University HospitalTuebingenGermany Department of Orthodontics Tuebingen University HospitalTuebingenGermany Department of Oral and Maxillofacial Surgery Tuebingen University HospitalTuebingenGermany

Background Various conservative and surgical approaches exist to treat Robin sequence(RS),but their effects on facial profile and mandibular catch-up growth are unclear.A functional treatment concept,used in our centre for 25 years,includes an individualized palatal plate with a velo-pharyngeal extension and intensive feeding *** We performed a prospective study to objectively describe facial profiles in infants with RS treated with the above *** with isolated RS were admitted to our tertiary perinatal and national referral centre for craniofacial malformations between May 2018 and Nov *** with RS received 3D-photographs during clinically indicated *** controls were recruited from Dec 2018 to Sep 2019 and received 3D-photographs every 3 *** digitally measured jaw index(JI),defined as alveolar overjet(O)x maxillary arch(U)/mandibular arch(L),and the soft tissue reference points A’-point,Nasion’,B’-point angle(ANB’),describing the relative position of maxilla to mandible,were *** mixed models were used to examine time trajectories in JI and ANB’.Results A total of 2073D images,obtained in 19 infants with RS and 32 controls,were *** and ANB’decreased over time in both groups[for JI−0.18(95%CI−0.25 to−0.10);for ANB’:−0.40°per month[(95%CI−0.48 to−0.32)]]but remained lower in controls[for JI−2.5(95%CI−3.2 to−1.8);for ANB’-1.7°(95%CI−2.4 to−1.0)].Also,the ANB’model showed a significant effect of the interaction term diagnosis x *** Based on longitudinal 3D images,we describe changes in objective parameters of facial profile in infants with and without RS during the first year of *** findings indicate catch-up growth in infants treated for RS.

关键词： Infant Mandibular catch-up growth Pre-epiglottic Baton Plate Robin sequence Three-dimensional photography

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Reproductive patterns in Araucaria araucana forests in the Andean range, Chile

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Ecological Processes 2024年第1期13卷 252-261页

作者： Sergio Donoso karen Pe?a-Rojas Claudia Espinoza Carolain Badaracco Rómulo Santelices-Moya Antonio Cabrera-Ariza Mediterranean forests Laboratory Faculty of Forestry and Nature Conservation Centro Secano Faculty of Agricultural and Forestry Sciences Universidad Católica del Maule Maule Advanced Studies Research Center Vice-Rector's Office for Research and Postgraduate Studies Catholic University of Maule

Background Araucaria araucana is a mast species that presents a high variability in annual cone production. Researchers have recorded synchronization events in cone production in different populations, which allows the seed production to be concentrated, reducing the percentage of seeds consumed by different animal *** We sampled three populations located in the Andes Mountains, Araucanía Region, Chile. In 2004 we began the collection of data on cone production, for which we installed permanent plots（1200 m2each） at each location. We identified and labeled each female tree in each plot to monitor its cone production. In 2012 we selected a total of 30 trees near the plots to evaluate the number of seeds per cone. In each february from 2012 to 2014 we selected two mature cones and covered them with a porous mesh for subsequent collection and storage in March. At the beginning of June, we counted and weighed the seeds, determining the average weight, the number of seeds per cone, the germination capacity（GC）, and the germination speed（GS）.Results Cone production was synchronous across the three locations. We observed significant differences among the locations and years evaluated. The cones had fewer seeds in 2013（high production） compared to those in 2012（low production）, but their weights were similar. In 2014 the cones produced smaller seeds in fewer quantities. The difference between the years 2013 and 2014 resulted from the high-energy expenditure in 2013. Regarding GC and GS, there were significant differences among the three locations（GC: f = 45.41, p f = 96.08, p f cones produced in a given year. These annual fluctuations in seed production are determining factors in the population dynamics of forest species. Our results allow a better understanding of the reproductive phenology of A. araucana and could hel

关键词： Seed weight Germination potential Masting species Sustainable use Dioecious species

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2023年美国麻醉医师学会神经肌肉阻滞监测与拮抗实践指南:美国麻醉医师学会工作组关于神经肌肉阻滞的报告

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中华麻醉学杂志 2024年第5期44卷 518-542页

作者： Stephan R.Thilen Wade A.Weigel Michael M.Todd Richard P.Dutton Cynthia A.Lien Stuart A.Grant Joseph W.Szokol Lars I.Eriksson Myron Yaster Mark D.Grant Madhulika Agarkar Anne M.Marbella Jaime f.Blanck karen B.Domino 西雅图华盛顿州明尼阿波里斯明尼苏达州达拉斯得克萨斯州密尔沃基威斯康星州教堂山北卡罗来纳州洛杉矶加利福尼亚州斯德哥尔摩瑞典丹佛科罗拉多州绍姆堡伊利诺斯州巴尔的摩马里兰州

本实践指南基于证据提供了全身麻醉期间及术后神经肌肉功能监测与阻滞拮抗的管理推荐。主要关注神经肌肉功能监测的类型与部位及阻滞拮抗的过程,以减少肌肉松弛药残留阻滞。

关键词：神经肌肉阻滞肌肉松弛药神经肌肉功能监测舒更葡糖钠新斯的明

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YAP activates pancreatic stellate cells and enhances pancreatic fibrosis

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Hepatobiliary & Pancreatic Diseases International 2022年第6期21卷 583-589页

作者： Lennard Spanehl Denis Revskij karen Bannert Luise Ehlers Robert Jaster Department of Medicine Ⅱ Division of GastroenterologyRostock University Medical CenterErnst-Heydemann-Str.618057 RostockGermany

Background:Pancreatic stellate cells(PSCs)foster the progression of pancreatic adenocarcinoma and chronic pancreatitis(CP)by producing a dense fibrotic ***,the incomplete knowledge of PSCs biology hampers the exploration of antifibrotic ***,we explored the role of the Hippo pathway in the context of PSCs activation and experimental ***:CP model was created in rats with the tail vein injection of dibutyltin dichloride(DBTC).The expression of Yes-associated protein(YAP)in CP tissue was *** and immortalized rats PSCs were treated with the YAP-inhibitor ***,YAP siRNA was ***,DNA synthesis,cell survival,levels ofα-smooth muscle actin(α-SMA)protein,presence of lipid droplets and PSCs gene expression were *** regulators of YAP signaling were studied by reporter gene ***:In DBTC-induced CP,pronounced expression of YAP in areas of tubular structures and periduc-tal fibrosis was *** diminished DNA replication in PSCs in a dose-dependent *** of YAP reduced cell *** cultures of PSCs were characterized by a de-crease of lipid droplets and increased synthesis ofα-SMA *** processes were not affected by *** the non-cytotoxic concentration of 100 nmol/L,verteporfin significantly reduced mRNA levels of transforming growth factor-β1(Tgf-β1)and Ccn family member 1(Ccn1).YAP signaling was acti-vated by TGf-β1,but repressed by interferon-γ.Conclusions:Activated YAP enhanced PSCs *** antifibrotic potential of Hippo pathway in-hibitors warrants further investigation.

关键词： Hippo pathway Pancreatic stellate cells fibrosis Pancreatic cancer Chronic pancreatitis

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A hybrid network integrating convolution and transformer for thymoma segmentation

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Intelligent Medicine 2023年第3期3卷 164-172页

作者： Jingyuan Li Wenfang Sun Xiulong feng karen Mvon Deneen Wen Wang Guangbin Cui Yi Zhang Center for Brain Imaging School of Life Science and TechnologyXidian UniversityXi'anShaanxi 710126China International Joint Research Center for Advanced Medical Imaging and Intelligent Diagnosis and Treatment&Xi'an Key Laboratory of Intelligent Sensing and Regulation of trans-Scale Life Information School of Life Science and TechnologyXidian UniversityXi'anShaanxi 710126China School of Aerospace Science and Technology Xidian UniversityXi'anShaanxi 710126China Department of Radiology Tangdu HospitalFourth Military Medical UniversityNo.4 Xinsi RoadXi'anShaanxi 710038China

Background Manual segmentation of thymoma is an onerous,labor-intensive,and subjective task for ***,the development of an automatic and efficient method for thymoma segmentation can be valuable for the early detection and diagnosis of this *** Three hundred and ten subjects were enrolled in this retrospective study and all underwent CECT *** the scans were manually labeled by four experienced *** successful application of convolution neural networks(CNNs)and Transformer in computer vision led us to propose a hybrid CNN–Transformer architecture,named transformer attention Net(TA-Net),that would allow the utilization of both local information from CNN features and the global information encoded by Transformers.U-Net was used as the basic structure and Transformers were inserted into convolution blocks in the *** addition,attention gates were embedded in skip connections to highlight salient *** of the accuracy,intersection over Union(IoU),Dice score,and Boundary f1 contour matching score(BfScore)between the predicted segmentation and the manual labels were utilized to evaluate segmentation *** for thymoma segmentation using TA-Net,the accuracy,Dice score,IoU,and BfScore were 92.49%,89.92%,83.80%,and 0.8945,respectively,and no significant differences were detected among tumor types and enhanced *** proposed method achieved the best performance when compared with state-of-the-art *** The proposed method,which combines CNNs with Transformer,achives outstanding performance in thymoma segmentation compared with previous ***-Net may provide consistent and reproducible delineation,thereby assisting radiologists in clinical applications.

关键词： Semantic segmentation Convolution neural network Transformer Attention mechanism Thymoma

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Bio/hydrochar Sorbents for Environmental Remediation

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Energy & Environmental Materials 2020年第4期3卷 453-468页

作者： Xingguang Zhang Yang Wang Junmeng Cai karen Wilson Adam f.Lee College of Chemical Engineering Nanjing Forestry UniversityNanjing 210037China Department of Chemistry School of ScienceUniversity of Shanghai for Science and TechnologyShanghai 200093China Biomass Energy Engineering Research Center School of Agriculture and BiologyShanghai Jiao Tong UniversityShanghai 200240China School of Science RMIT UniversityMelbourne VIC 3000Australia

A rising global population and aspirational higher living standards has driven a step change in resource utilization and concomitant anthropogenic pollution across the ***-cost and scalable technologies for environmental remediation are therefore urgently sought,with an emphasis on trash-to-treasure strategies that exploit abundant but underutilized waste by-products of existing *** are carbon-rich,porous solids produced by biomass pyrolysis under anaerobic or oxygen-scarce conditions at high temperature(350–700°C),while hydrochars are produced by hydrothermal biomass carbonization at lower temperature(130–250°C)and high autogenous pressures(0.3–4.0 MPa).Bio/hydrochars possess unique physicochemical properties,notably high surface areas(100–1500 m2 g-1)and porosity(0.25–2.5 cm^(3)g^(-1))and rich surface chemistry featuring carboxylic,phenolic,hydroxyl,and carbonyl functions,amenable to chemical,physical,or biochemical modification,rendering them ideal sorbents for pollutants such as heavy metals(*** and Cr),and toxic organic(e.g.,dyes and xenobiotics)and inorganic(e.g.,SO_(2))***/hydrochars are attractive for environmental remediation of pollutant mixtures by surface complexation,redox chemistry,electrostatic interactions/ion exchange,or *** review discusses recent opportunities and challenges in creating bio/hydrochar sorbents and their nanocomposites through grafting,doping,and chemical/physical activation,for the depollution of aquatic and atmospheric environments.

关键词： adsorption biochar environmental remediation hydrochar

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The effectiveness of shared clinical teaching in nursing

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International Journal of Nursing Sciences 2019年第2期6卷 211-215页

作者： karen f.Phillips Lizy Mathew Nadine Aktan Priyadharshiny Sandanapitchai Department of Nursing College of Science and HealthWilliam Paterson University300 Pompton RdWayneNJ07470USA Department of Psychology College of Humanities and ScienceWilliam Paterson University300 Pompton RdWayneNJ07470USA

Aim: Clinical learning is a critical component of a nursing *** satisfaction in clinical environment is crucial to foster a positive learning *** shortages have made clinical teaching more challenging;as such,alternate models of clinical teaching must be explored by nursing *** purpose of this study was to measure the perception of student satisfaction in regard tothe effectiveness of shared clinical teaching in ***: Utilizing Chan's Clinical Learning Environment Inventory (CLEI),this quantitative comparative study examined nursing students' preferred and actual clinical learning *** CLEI contains 42 questions in six subscales: Individualization,Innovation,Involvement,Personalization,Task Orientation,and Satisfaction in both the actual and preferred clinical learning *** sample consisted of 202 nursing students in two groups: the first group had 91 students who experienced shared clinical teaching with two faculty,whereas the second group had 111 students who experienced the traditional,single faculty *** results were analyzed using independent sample ***: The preferred learning environment was rated highest in all six *** of the Satisfaction subscale and the Innovation subscale for Actual Learning Environment,and the score of the Innovation subscale for Preferred Learning Environment of students experiencing shared clinical teaching with two faculty were higher than the scores of students experiencing traditional,single faculty model,with statistically significance (Pferred the shared clinical teaching model with two faculty over the single faculty *** programs can utilize this model and apply these results to develop and maintain quality clinical teaching.

关键词： Clinical Clerkship Clinical learning environment faculty shortage Nursing education Students Nursing Questionnaires

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Clinical education and student satisfaction: An integrative literature review

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International Journal of Nursing Sciences 2017年第2期4卷 205-213页

作者： karen f.Phillips Lizy Mathew Nadine Aktan Bryant Catano Department of Nursing College of Science and Health William Paterson University 300 Pompton Rd Wayne NJ 07470 USA Department of Biology College of Science and Health William Paterson University 300 Pompton Rd Wayne NJ 07470 USA

The clinical component of undergraduate clinical education is a critical area in nursing programs. faculty shortages have made recruitment of clinical faculty and clinical teaching more challenging. As such, alternate models of clinical faculty assignments are being explored to address faculty shortages. This article contains an extensive literature review conducted to survey models of clinical education and student satisfaction with the clinical environment. The purpose of this paper is to examine student satisfaction in the clinical learning environment using articles employing the Clinical Learning Envi-ronment Inventory (CLEI) along with examining the use of alternate clinical staffing models in differing levels of undergraduate nursing students. A literature search focusing on studies published between 2002 and 2015 was conducted from 5 electronic databases. Thirty-five articles were reviewed and 22 were selected for this literature review. The studies reviewed concluded that students favored a more positive and favorable clinical environment than they perceived as being actually present. A supportive clinical learning environment is of paramount importance in securing positive teaching learning out-comes. Nurse educators can apply the results of this review in order to develop and maintain quality clinical teaching and to promote a positive, student-centric, clinical learning environment.

关键词： Clinical Clinical learning environment Education Student satisfaction

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Novel material tailoring method for internally pressurized fG spherical and cylindrical vessels

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Acta Mechanica Sinica 2018年第5期34卷 936-948页

作者： fayyaz Nosouhi Dehnavi Ali Parvizi karen Abrinia School of Mechanical Engineering College of EngineeringUniversity of TehranTehranIran

A new material tailoring method for spherical and cylindrical vessels made of functionally graded materials (fGMs) is *** is assumed that the fG material is composed of an A1-SiC metallic-matrix composite.A uniform ratio of inplane shear stress to yield strength [φ(r)] is used as the design criterion to utilize the maximum capacity of the *** aim is to find a distribution of SiC particles in the radial direction,i.e.,f(r),that achieves a uniform index φ(r) =const,through the wall thickness of the internally pressurized spherical or cylindrical *** the Mori-Tanaka and rule-of-mixtures homogenization schemes are used to express the effective elastic module and Poisson's ***,the strength of the composite is expressed based on the rule of ***,finite element simulation is carried out to verify the accuracy of the analytical *** effects of input parameters such as the internal pressure,strength of the SiC particles,ratio of in-plane shear stress to effective yield strength,and choice of homogenization scheme on the tailored distribution of the SiC volume fraction in the radial direction are also investigated.

关键词： Material tailoring Sphere Cylinder fGM Mori-Tanaka Rule of mixtures

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