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High levels of psychosocial distress among Australian frontline healthcare workers during the COVID-19 pandemic: a cross- sectional survey

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General Psychiatry 2021年第5期34卷 31-41,I0006-I0025页

作者： Natasha Smallwood Leila Karimi Marie Bismark Mark Putland Douglas Johnson Shyamali Chandrika Dharmage elizabeth Barson Nicola Atkin Claire Long Irene Ng Anne Holland Jane e Munro Irani Thevarajan Cara Moore Anthony McGillion Debra Sandford karen Willis Department of Respiratory Medicine Alfred HospitalPrahranVictoriaAustralia Department of Allergy Immunology and Respiratory MedicineCentral Clinical SchoolAlfred HospitalMonash UniversityMelbourneVictoriaAustralia School of Psychology and Public Health La Trobe UniversityMelbourneVictoriaAustralia School of Medicine and Healthcare Management Caucasus UniversityTbilisiGeorgia Department of Psychiatry The Royal Melbourne HospitalParkvilleVictoriaAustralia Department of Public Health Law Melbourne School of Population and Global HealthThe University of MelbourneParkvilleVictoriaAustralia Department of emergency Services The Royal Melbourne HospitalParkvilleVictoriaAustralia Department of Critical Care Faculty of MedicineDentistry and Health SciencesThe University of MelbourneMelbourneVictoriaAustralia Departments of General Medicine and Infectious Diseases The Royal Melbourne HospitalParkvilleVictoriaAustralia Department of Medicine The Royal Melbourne HospitalThe University of MelbourneParkvilleVictoriaAustralia Allergy and Lung Health Unit School of Population and Global HealthThe University of MelbourneParkvilleVictoriaAustralia Department of Allied Health The Royal Melbourne HospitalParkvilleVictoriaAustralia Parkville Integrated Palliative Care Service The Royal Melbourne HospitalParkvilleVictoriaAustralia Sir Peter MacCallum Department of Oncology The University of MelbourneParkvilleVictoriaAustralia Department of Geriatric Medicine Western HealthFootscrayVictoriaAustralia Department of Anaesthesia and Pain Management The Royal Melbourne HospitalParkvilleVictoriaAustralia Centre for Integrated Critical Care Melbourne Medical SchoolThe University of MelbourneParkvilleVictoriaAustralia Department of Physiotherapy Alfred HealthMelbourneVictoriaAustralia Rheumatology Unit Royal Children's HospitalParkvilleVictoriaAustralia Arthritis and Rheumatology Murdoch Children's Research InstituteParkvilleVictoriaAustralia Department of Infectious Diseases T

Background The coronavirus disease 2019(COVID-19)pandemic has had a profound and prolonged impact on healthcare services and healthcare *** The Australian COVID-19 Frontline Healthcare Workers Study aimed to investigate the severity and prevalence of mental health issues,as well as the social,workplace and financial disruptions experienced by Australian healthcare workers during the COVID-19 *** A nationwide,voluntary,anonymous,single timepoint,online survey was conducted between 27 August and 23 October *** self-identifying as frontline healthcare workers in secondary or primary care were invited to *** were recruited through health organisations,professional associations or colleges,universities,government contacts and national ***,home and work situation,health and psychological well-being data were *** A total of 9518 survey responses were received;of the 9518 participants,7846(82.4%)participants reported complete *** regard to age,4110(52.4%)participants were younger than 40 years;6344(80.9%)participants were *** were nurses(n=3088,39.4%),doctors(n=2436,31.1%),allied health staff(n=1314,16.7%)or in other roles(n=523,6.7%).In addition,1250(15.9%)participants worked in primary *** measured mental health symptoms were common:mild to severe anxiety(n=4694,59.8%),moderate to severe burnout(n=5458,70.9%)and mild to severe depression(n=4495,57.3%).Participants were highly resilient(mean(SD)=3.2(0.66)).Predictors for worse outcomes on all scales included female gender;younger age;pre-existing psychiatric condition;experiencing relationship problems;nursing,allied health or other roles;frontline area;being worried about being blamed by colleagues and working with patients with *** The COVID-19 pandemic is associated with significant mental health symptoms in frontline healthcare *** preparedness together with policies and pra

关键词： distress nurse workers

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Two Sets of Acupoint Combination of Similar Functions engage Shared Neural Representation:A Functional Magnetic Resonance Imaging Study

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Chinese Journal of Integrative Medicine 2014年第3期20卷 184-193页

作者：赵凌秦伟刘继欣方莉董明皓张富文蒋萃孙金铂 karen.M.von Deneen 梁繁荣 College of Acupuncture and Tuina Chengdu University of Traditional Chinese Medicine Life Sciences Research Center School of Life Sciences and TechnologyXidian University The Third Affiliated Hospital of Zhejiang University of Traditional Chinese Medicine The Teaching Hospital of Chengdu University of Traditional Chinese Medicine

Objective： To investigate whether the neural representations underlying alternating two acupoint combinations （ACs） are the same or not. Methods： In this functional magnetic resonance imaging study, two sets of analgesia ACs were utilized, including Waiguan （Te5） and Qiuxu （GB40） for Group A, and Neiguan （PC6） and Taichong （LR3） for Group B, which are the most commonly adopted in clinical treatment. each group had 20 healthy subjects. An experinlental design was proposed, which consisted of a pre-needling resting phase, a needling phase and a post-needling resting phase. This paradigm optimally mimics the clinical protocol as well as focuses on both the stimulation and the resting periods. The results were subjected to general linear model analysis, conjunction analysis and the functional connectivity analysis. Results： The rostral anterior cingulated cortex was engaged in the initiative period of the acupuncture effect in both groups, and it was chosen as the seed region for the functional connectivity analysis for the following resting period. The results showed that several shared brain regions were involved in both groups, in particular the insula, amygdala and hypothalamus. Moreover, significant differences were located at the posterior cingulated cortex as revealed by a two sample t-test （P〈0.05, corrected）. Other regions showed no significant differences. This finding was further supported by the spatial correlation analysis that the two groups were significantly correlated （r=0.51, P〈0.01）. Conclusion： This preliminary research helps us understand the neurophysiological mechanisms of acupuncture when following clinical guidelines on ACs, as well as provides an important opportunity to develop better treatment strategies for reducing, or even preventing pain.

关键词： acupoint combination acupuncture analgesia functional magnetic resonance imaging

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鸡肉的健康作用和人类饮食模式

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中国家禽 2008年第13期30卷 58-60页

作者： karen e.Charlton Yasmine C.Probst Linda C.Tapsell

本文是澳大利亚昆士兰州第一产业和渔业部动物研究所和伍伦贡大学美食中心的一篇报告摘要,原题为"食品、健康和营养:仔鸡作用在哪?"报告以鸡肉和澳大利亚人的健康为例,从各个层面介绍了鸡肉对健康的帮助,并引发一个思考:什么样的鸡肉是... 详细信息

本文是澳大利亚昆士兰州第一产业和渔业部动物研究所和伍伦贡大学美食中心的一篇报告摘要,原题为"食品、健康和营养:仔鸡作用在哪?"报告以鸡肉和澳大利亚人的健康为例,从各个层面介绍了鸡肉对健康的帮助,并引发一个思考:什么样的鸡肉是最好的?

关键词：鸡肉饮食模式澳大利亚动物研究所第一产业昆士兰州

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Undergraduate nursing students' knowledge of aging, attitudes toward and perceptions of working with older adults in Kathmandu Nepal

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International Journal of Nursing Sciences 2019年第2期6卷 204-210页

作者： Saruna Ghimire Nitisha Shrestha karen e.Callahan Dhirendra Nath Binaya Kumar Baral Nirmala Lekhak Devendra Raj Singh Agrata Health and education(AHeAD)-Nepal KathmanduNepal School of Public Health University of NevadaLas VegasUSA Asian College for Advanced Studies Purbanchal UniversityLalitpurNepal Southeast Asia Development Actions Network(SADAN) LalitpurNepal Department of Biochemistry Nepal Medical College and Teaching HospitalKathmanduNepal School of Nursing University of NevadaLas VegasUSA

Objectives: This study aims to examine Nepalese undergraduate nursing students' knowledge of aging,attitudes towards older adults and perceptions of working with older adults,and to assess differences in these outcomes by socio-demographic characteristics as well as type of nursing ***: A cross-sectional study was conducted among 385 undergraduate nursing students in six nursing colleges located in the Kathmandu Valley,Knowledge of aging,attitudes toward older adults and perceptions of working with older adults were assessed using standardized tools,the Palmore Facts on Aging Quiz,Kogan's Attitudes towards Older People Scale,and Nolan's Intent to Work with Older People Questionnaire,***: The mean knowledge scores on older adults and aging were relatively low;participants scored an average of 26.9 out of *** assessing attitudes towards and perceptions of working with older adults were more *** to students pursuing a Bachelor of Science in Nursing (BSN),students pursuing a Bachelor of Nursing (BN) had a significantly higher score on the knowledge,attitudes and perception of aging *** regression analyses showed that the students' knowledge of aging (β =0.55;95% CI =0.25-0.86) and perceptions of working with older adults (β =0.22;95% Cl =0.05 -0.38) had a significant positive association with their attitudes toward older ***: Undergraduate nursing students in the Kathmandu Valley of Nepal displayed a relatively low level of knowledge,but a positive attitude towards older adults,and a positive perception of working with older *** differences in knowledge,attitude,and perception scores between students in BSN and BN programs needs further investigation;closing this gap may be important for bolstering undergraduate gerontological preparation in Nepal.

关键词： Aged Attitudes Knowledge Perceptions Students Nursing Nepal

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Lactobacilli,bifi dobacteria and *** nissle induce pro-and anti-inflammatory cytokines in peripheral blood mononuclear cells

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World Journal of Gastroenterology 2006年第37期12卷 5978-5986页

作者： Ulf Helwig karen M Lammers Fernando Rizzello Patricia Brigidi Verena Rohleder elisabetta Caramelli Paolo Giochetti Juergen Schrezenmeir Ulrich R Foelsch Stefan Schreiber Massimo Campieri University Hospital Schleswig-Holstein Campus Kiel 1~(st) Medical Department Schittenhelmstr.12 24105 Kiel Germany University of Bologna Department of Internal Medicine and Gastroenterology Policlinico S. Orsola Via Massarenti 9 40138 Bologna Italy University of Bologna Department of Internal Medicine and Gastroenterology Policlinico S. Orsola Via Massarenti 9 40138 Bologna Italy University of Bologna Institute for Microbiology Via Bellmeloro 9 40138 Bologna Italy University Hospital Schleswig-Holstein Campus Kiel 1~(st) Medical Department Schittenhelmstr.12 24105 Kiel Germany University of Bologna Histology and General Embryology and Pharmaceutical Science Via Bellmeloro 9 40138 Bologna Italy Federal Research Center for Nutrition and Food Location Kiel Institute for Physiology and Bioche- mistry of Nurtition Hermann-Weigmann-Str.1 24103 Kiel Germany

AIM： To investigate whether the stimulation of peripheral blood mononuclear cells （PBMNC） with the cell debris and cell extraction of different probiotic strains is similar or species specific. MeTHODS： Three strains of bifidobacteria, 4 strains of lactobacilli, and e. coli nissle were sonicated and centrifuged in order to divide them into cell extract and cell debris. PBMNC were separated by density gradient and incubated for 36 h with either the cell debris or the cell extract of single strains of probiotic bacteria in doses from 10^2 to 10^8 CFU/mL. Cell supernatants were taken and interleukin （IL）-10, IL-1β, and tumor necosis factor （TNF）-α were determined by eLISA. ReSULTS： Depending on the species super-family, the strains had different stimulation patterns. except for both L. casei strains, the cell extract of bitTdobacteria and/actobacilli had less stimulating capacity than cell debris, whereas the cell extract of e. coli nissle had similar stimulating properties to that of the cell debris of the strain and significantly more stimulating capacity than that of bifidobacteria and lactobacilli. The cell debris of bifidobacteria stimulated more cytokine release than the cell debris of lactobacilli. The cell debris of lactobacilli did not have a stimulating capacity when lower concentrations were used. Neither cell extraction nor cell debris had an inhibitory effect on the production of the tested cytokines by stimulated PBMNC. CONCLUSION： The incubation of probiotic strains, which have been used in clinical trials for inflammatory diseases, with immunocompetent cells leads to different species specific reactions. High IL-10 response to cell debris of bifidobacteria and e. coli nissle can be found. This corresponds to positive effects of bihdobacteria and e. coli nissle in clinical trials for inflammatory bowel disease compared to negative outcomes obtained with lactobacilli.

关键词： Lactobacilli Bifidobacteria Probiotics Interleukin-l0 Tumor necrosis factor-α Interleukin-1β Peripheral blood mononuclear cells

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使用大脑兴奋剂时十个护理要点

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国际护理学杂志 1987年第1期08卷 27-28页

作者： karen e Burgess ,郭玉贞

1.注意症状和行为症状以及中枢和交感神经过度兴奋的症状。体征包括:节律障碍、高血压、心动过速、震颤和胃肠功能紊乱。行为症状包括:焦虑、易激动、不安定、失眠、精力不集中和个性改变。2.对同时需要给于两种大脑兴奋剂的任何医嘱都... 详细信息

1.注意症状和行为症状以及中枢和交感神经过度兴奋的症状。体征包括:节律障碍、高血压、心动过速、震颤和胃肠功能紊乱。行为症状包括:焦虑、易激动、不安定、失眠、精力不集中和个性改变。2.对同时需要给于两种大脑兴奋剂的任何医嘱都要询问一下,如果医嘱确实指出需要,剂量应比平时所给的治疗量减少,以补偿药物的添加作用。3.鼓励病人减少咖啡因的摄取,可能的话从饮食中全部消除。囚为咖啡因和另一种大脑兴奋剂结合可能会过度刺激中枢和交感神经系统。4.劝告病人就寝前6小时以内不能使用大脑兴奋剂。因为,放射过敏吸附(RAS)活性增加和大脑皮质兴奋性增强会使大脑很难在夜间入睡。

关键词：行为症状剂量大脑用药量病人儿童

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Response of Hypercholesterolemic Rats to Sesamum indicum Linn Seed Oil Supplemented Diet

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Journal of Life Sciences 2012年第11期6卷 1214-1219页

作者： Olubunmi Bolanle Ajayi James Braimoh karen Olasunkanmi Department of Biochemistry Faculty of Sciences Ekiti State University P.M.B. 5363 Ado-Ekiti Nigeria

Fatty acid composition of Sesamum indicum oil was determined by gas chromatography and the response of hypercholesterolemic rats to diet supplemented with Sesamum indicum seed oil was investigated. Twenty four rats weighing between 120-130 g were randomly assigned into four groups. Group A was fed normal diet, Group B, C and D were fed hypercholesterolemic diet （i.e. 20% fat ＋ 1% cholesterol） for two weeks to establish hypercholesterolemia. Group B were maintained on hyper diet while C and D were fed 5% and 10% benniseed oil supplemented diet for four weeks. Plasma was collected and analyzed for TC （total cholesterol）, HDL-C （high density lipoprotein）, LDL-C （low density lipoprotein） and TG （triglycerides） levels. Linoleic acid （42.44%） and oleic acid （40.60%） were the major unsaturated fatty acid in the oil. Significant increase （P 〈 0.05） was observed in the TC, TG, LDL and LDL/HDL ratio of the hypercholesterolemic rats compared to the normal control. Supplementation with Sesamum indicum seed oil at 5% and 10% levels resulted in significant decrease （P 〈 0.05） in TC, TG, LDL and LDL/HDL ratio, and significant increase in the HDL-C. The high level of unsaturated fatty acid in the oil may in part be responsible for the hypocholesterolemic effect of the oil.

关键词： Sesamum indicum fatty acid composition hypercholesterolemia lipid profile.

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Structural analysis of a nanoparticle containing a lipid bilayer used for detergent-free extraction of membrane proteins

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Nano Research 2015年第3期8卷 774-789页

作者： Mohammed Jamshad Vinciane Grimard Ilaria Idini Tim J. Knowles Miriam R. Dowle Naomi Schofield Pooja Sridhart Yupin Lin Rachael Finka Mark Wheatley Owen R. T. Thomas Richard e. Palmerr Michael Overduin Cedric Govaerts Jean-Marie Ruysschaert karen J. edler Tim R. Dafforn School of Biosciences University of Birmingham Edgbaston Birmingham B 15 2TT UK Universite Libre de Bruxelles SFMB - CP206/2 Bd. du Triomphe Acces 2 1050 Bruxelles Belgium Department of Chemistry University of Bath Claverton Down Bath BA2 7AY UK School of Cancer Studies University of Birmingham Edgbaston Birmingham B 15 2 TT UK Nanoscale Physics Research Laboratory and PSIBS School of Physics and Astronomy University of Birmingham Edgbaston Birmingham B 15 2TT UK School of Chemical engineering University of Birmingham Edgbaston Birmingham B 15 2TT UK

In the past few years there has been a growth in the use of nanoparticles for stabilizing lipid membranes that contain embedded proteins. These bionanoparticles provide a solution to the challenging problem of membrane protein isolation by maintaining a lipid bilayer essential to protein integrity and activity. We have previously described the use of an amphipathic polymer （poly（styrene-co-maleic add）, SMA） to produce discoidal nanoparticles with a lipid bilayer core containing the embedded protein. However the structure of the nanoparticle itself has not yet been determined. This leaves a major gap in understanding how the SMA stabilizes the encapsulated bilayer and how the bilayer relates physically and structurally to an unencapsulated lipid bilayer. In this paper we address this issue by describing the structure of the SMA lipid particle （SMALP） using data from small angle neutron scattering （SANS）, electron microscopy （eM）, attenuated total reflection Fourier transform infrared spectroscopy （ATR-FTIR）, differential scanning calorimetry （DSC） and nuclear magnetic resonance spectroscopy （NMR）. We show that the particle is disc shaped containing a polymer ＂bracelet＂ encircling the lipid bilayer. The structure and orientation of the individual components within the bilayer and polymer are determined showing that styrene moieties within SMA intercalate between the lipid acyl chains. The dimensions of the encapsulated bilayer are also determined and match those measured for a natural membrane. Taken together, the description of the structure of the SMALP forms the foundation for future development and applications of SMALPs in membrane protein production and analysis.

关键词： nanoparticles lipid polymer membrane proteins structure detergent

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Antibiotic-loaded phosphatidylcholine inhibits staphylococcal bone infection

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World Journal of Orthopedics 2016年第8期7卷 467-474页

作者： Jessica Amber Jennings karen e Beenken Robert A Skinner Daniel G Meeker Mark S Smeltzer Warren O Haggard karen S Troxel Department of Biomedical engineering University of MemphisMemphisTN 38152United States Department of Microbiology and Immunology University of Arkansas Medical SciencesLittle RockAR 72205United States Jamesport NY 11947United States Zimmer Biomet WarsawIN 46581United States

AIM: To test antibiotic-loaded coating for efficacy in reducing bacterial biofilm and development of osteomyelitis in an orthopaedic model of implant infection. MeTHODS: Phosphatidylcholine coatings loaded with 25% vancomycin were applied to washed and sterilized titanium wires 20 mm in length. A 10 mm segment was removed from rabbit radius(total = 9; 5 coated, 4 uncoated), and the segment was injected with 1 × 106 colony forming units(CFUs) of Staphylococcus aureus(UAMS-1 strain). Titanium wires were inserted throughthe intramedullary canal of the removed segment and into the proximal radial segment and the segment was placed back into the defect. After 7 d, limbs were removed, X-rayed, swabbed for tissue contamination. Wires were removed and processed to determine attached CFUs. Tissue was swabbed and streaked on agar plates to determine bacteriological ***: Antibiotic-loaded coatings resulted in significantly reduced biofilm formation(4.7 fold reduction in CFUs; P es and reduced bacteriological score in surrounding tissue(4.0 ± 0 for uncoated, 1.25 ± 0.5 for coated; P = 0.01). Swelling and pus formation was evident in uncoated controls at the 7 d time point both visually and radiographically, but not in antibiotic-loaded ***: Active antibiotic was released from coated implants and significantly reduced signs of osteomyelitic symptoms. Implant coatings were well tolerated in bone. Further studies with additional control groups and longer time periods are warranted. Antibiotic-loaded phosphatidylcholine coatings applied at the point of care could prevent implant-associated infection in orthopaedic defects.

关键词： Biofilm Implant Drug delivery coating Antibiotic Orthopaedic infection

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Novel strategy for disease risk prediction incorporating predicted gene expression and DNA methylation data:a multi-phased study of prostate cancer

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Cancer Communications 2021年第12期41卷 1387-1397页

作者： Chong Wu Jingjing Zhu Austin King Xiaoran Tong Qing Lu Jong Y.Park Liang Wang Guimin Gao Hong-Wen Deng Yaohua Yang karen e.Knudsen Timothy R.Rebbeck Jirong Long Wei Zheng Wei Pan David V.Conti Christopher A Haiman Lang Wu Department of Statistics Florida State UniversityTallahasseeFL 32304USA Cancer epidemiology Division Population Sciences in the Pacific ProgramUniversity of Hawaii Cancer CenterUniversity of Hawaii at ManoaHonoluluHI 96813USA Department of epidemiology and Biostatistics Michigan State UniversityEast LansingMI 48824USA Department of Biostatistics University of FloridaGainesvilleFL 32603USA Department of Cancer epidemiology H.Lee Moffitt Cancer Center and Research InstituteTampaFL 33612USA Department of Tumor Biology H.Lee Moffitt Cancer Center and Research InstituteTampaFL 33612USA Department of Public Health Sciences University of ChicagoChicagoIL 60637USA Center of Bioinformatics and Genomics Department of Global Biostatistics and Data ScienceTulane UniversityNew OrleansLA 70112USA Division of epidemiology Department of MedicineVanderbilt Epidemiology CenterVanderbilt-Ingram Cancer CenterVanderbilt University Medical CenterNashvilleTN 37203USA Department of Cancer Biology Sidney Kimmel Cancer CenterThomas Jefferson UniversityPhiladelphiaPA 19107USA Department of Medical Oncology Dana-Farber Cancer InstituteBostonMA 02215USA Department of epidemiology Harvard TH Chan School of Public HealthBostonMA 02115USA Division of Biostatistics University of MinnesotaMinneapolisMN 55455USA Department of Preventive Medicine Keck School of MedicineUniversity of Southern California/Norris Comprehensive Cancer CenterLos AngelesCA 90033USA

Background:DNA methylation and gene expression are known to play important roles in the etiology of human diseases such as prostate cancer(PCa).However,it has not yet been possible to incorporate information of DNA methylation and gene expression into polygenic risk scores(PRSs).Here,we aimed to develop and validate an improved PRS for PCa risk by incorporating genetically predicted gene expression and DNA methylation,and other genomic information using an integrative ***:Using data from the PRACTICAL consortium,we derived multiple sets of genetic scores,including those based on available single-nucleotide polymorphisms through widely used methods of pruning and thresholding,LDpred,LDpred-funt,AnnoPred,and eBPRS,as well as PRS constructed using the genetically predicted gene expression and DNA methylation through a revised pruning and thresholding *** the tuning step,using the UK Biobank data(1458 prevalent cases and 1467 controls),we selected PRSs with the best *** an independent set of data from the UK Biobank,we developed an integrative PRS combining information from individual ***,in the testing step,we tested the performance of the integrative PRS in another independent set of UK Biobank data of incident cases and ***:Our constructed PRS had improved performance(C statistics:76.1%)over PRSs constructed by individual benchmark methods(from 69.6%to 74.7%).Furthermore,our new PRS had much higher risk assessment power than family *** overall net reclassification improvement was 69.0%by adding PRS to the baseline model compared with 12.5%by adding family ***:We developed and validated a new PRS which may improve the utility in predicting the risk of developing *** innovative method can also be applied to other human diseases to improve risk prediction across multiple outcomes.

关键词： risk prediction polygenic risk scores predicted gene expression predicted DNA methylation integrative models prostate cancer

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