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Confirming Stereochemical Structures of Strigolactones Produced by Rice and Tobacco

Molecular Plant 2013年第1期6卷 153-163页

作者： Xiaonan Xie kaori Yoneyama Takaya Kisugi Kenichi Uchida Seisuke Ito Kohki Akiyama Hideo Hayashi Takao Yokota Takahito Nomura Koichi Yoneyama Weed Science Center Utsunomiya University 350 Mine-machi Utsunomiya 321-8505 Japan Department of Biosciences Faculty of Science and Technology Teikyo University 1-1 Toyosatodai Utsunomiya 320-8851 Japan Graduate School of Life and Environmental Sciences Osaka Prefecture University 1-1 Gakuen-cho Nakaku Sakai Osaka 599-8531 Japan

Major strigolactones （SLs） produced by rice （Oryza sativa L. cv. Nipponbare） and tobacco （Nicotiana tabacum L. cv. Michinoku No. 1） were purified and their stereochemical structures were determined by comparing with optically pure synthetic standards for their NMR and CD data and retention times and mass fragmentations in ESI-LC/MS and GC-MS. SLs purified from root exudates of rice plants were orobanchol, orobanchyl acetate, and ent-2＂-epi-5-deoxystr- igol. In addition to these SLs, 7-oxoorobanchyl acetate and the putative three methoxy-5~deoxystrigol isomers were detected by LC-MS/MS. The production of 7-oxoorobanchyl acetate seemed to occur in the early growth stage, as it was detected only in the root exudates collected during the first week of incubation. The root exudates of tobacco contained at least 11 SLs, including solanacol, solanacyl acetate, orobanchol, ent-2＂-epi-orobanchol, orobanchyl acetate, ent-2＇- epi-orobanchyl acetate, 5-deoxystrigol, ent-2＂-epi-5-deoxystrigol, and three isomers of putative didehydro-orobanchol whose structures remain to be clarified. Furthermore, two sorgolactone isomers but not sorgolactone were detected as minor SLs by LC-MS/MS analysis. It is intriguing to note that rice plants produced only orobanchol-type SLs, derived from ent-2＂-epi-5-deoxystrigol, but both orobanchol-type and strigol-type SLs, derived from 5-deoxystrigol were detected in tobacco plants.

关键词： germination stimulant Orobanche rice root parasitic plant Striga strigolactone tobacco.

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Relationship of Clinical Nursing Competence to Nursing Occupational Experience in Hospice/Palliative Care Nurses in Japan

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Health 2015年第2期7卷 279-288页

作者： kaori Tsutsumi Keiko Sekido Department of Nursing Kobe University Graduate School of Health Sciences Kobe Japan

Aim: The purpose of this research is to examine the relationship between the clinical nursing competence and nursing occupational experience in hospice/palliative care nurses (HPN) in Japan. Methods: A mail survey using an anonymous self-administered questionnaire was conducted on clinical nursing competence regarding communication, care and prediction of worsening of symptoms with the authors’ previous research as a framework. The subjects were nurses working in hospice/palliative care units in Japan, and the period of survey was January to February, 2014. Results: In this study, the relationship between the clinical nursing competence and nursing occupational experience of HPNs in hospice/palliative care which was clarified in the authors’ previous research was examined. The results showed that the amount of clinical nursing experience as a nurse was related to clinical nursing competence in the care of end-of-life patients and worsening of symptoms. The abundance of specialized nursing experience was also related to clinical nursing competence in communication in addition to care and prediction of worsening of symptoms. Training experience was also shown to be effective for hospice/palliative care. Conclusion: From these results, clinical nursing competence of HPNs in care of end-of-life patients and prediction of worsening of symptoms are possibly learned through the accumulation of clinical nursing experience as a nurse. However, HPN’s clinical nursing competence in communication was suggested to be developed through nursing practice in hospice/palliative care.

关键词： Hospice and Palliative Care Nursing Clinical Nursing Competence Nursing Occupational Experience

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Non-medical financial burden in tuberculosis care: a cross-sectional survey in rural China

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Infectious Diseases of Poverty 2016年第1期5卷 37-43页

作者： Qiang Li Weixi Jiang Quanli Wang Yuan Shen Jingyuan Gao kaori D.Sato Qian Long Henry Lucas Department of Biostatistics and Epidemiology School of Public HealthXi’an Jiaotong UniversityXi’anShaanxiChina Global Health Research Center Duke Kunshan UniversityKunshanJiangsuChina Duke Global Health Institute Duke UniversityDurhamNCUSA Institute of Development Studies at the University of Sussex BrightonUK

Background:Treatment of tuberculosis(TB)in China is partially covered by national programs and health insurance schemes,though TB patients often face considerable medical *** some,especially those from poorer households,non-medical costs,such as transport,accommodation,and nutritional supplementation may be a substantial additional *** this article we aim to evaluate these non-medical costs induced by seeking TB care using data from a large scale cross-sectional ***:A total of 797 TB cases from three cities were randomly selected using a stratified cluster sampling *** medical costs,outpatient medical costs,and direct non-medical costs related to TB treatment were collected using in-person interviews by trained *** and median non-medical costs for different sub-groups were calculated and compared using Kruskal-Wallis and Mann–Whitney U *** analysis was conducted to assess the influence of different patient characteristics on total non-medical ***:The median non-medical cost was RMB 1429,with interquartile range RMB 424–*** median non-medical costs relating to inpatient treatment,outpatient treatment,and additional nutrition supplementation were RMB 540,91,and 900,*** the 797 cases,20%reported catastrophic expenditure on non-medical *** significant differences were detected between different cities,age groups,geographical locations,inpatient/outpatient care,education levels and family income ***:Non-medical costs relating to TB treatment are a serious financial burden for many TB *** assistance that can limit this burden is urgently needed during the treatment period,especially for the poor.

关键词： Non-medical cost Financial burden Tuberculosis China

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Endoscopic resection for residual lesion of metastatic gastric cancer:A case report

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World Journal of Clinical Cases 2019年第4期7卷 482-488页

作者： kaori Hayashi Sho Suzuki Hisatomo Ikehara Hiroaki Okuno Akira Irie Mitsuru Esaki Chika Kusano Takuji Gotoda Mitsuhiko Moriyama Division of Gastroenterology and Hepatology Department of Medicine Nihon University School of Medicine

BACKGROUND Chemotherapy is a standard strategy for stage IV gastric cancer ***, some cases cannot undergo conversion surgery because of their frailty,even if the patients had response to chemotherapy. For these patients, local tumor progression is a problem. We report here the case of a patient whose residual gastric cancer was resected through endoscopic submucosal dissection(ESD)after concomitant chemotherapy for metastatic gastric *** SUMMARY An 85-year-old male complained of difficulty swallowing, and examination revealed gastric cancer with multiple liver metastases. Although he received concomitant chemotherapy, a residual tumor was observed in the primary lesion while the metastatic lesions disappeared completely. Conversion surgery was considered optional treatment; however, he could not undergo that because of advanced age and comorbidities. Thus, we performed ESD to treat the residual tumor. As a result, we resected the residual lesion completely. The patient has been alive for 29 mo since ESD, without *** We achieved local control using ESD, and these findings may provide therapeutic improvements both in local control and patient survival outcomes.

关键词： Gastric cancer Liver metastases Conversion Endoscopic submucosal dissection Chemotherapy Case report

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Use of anti-tuberculosis drugs among newly diagnosed pulmonary tuberculosis inpatients in China: a retrospective study

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Infectious Diseases of Poverty 2016年第1期5卷 9-14页

作者： Fei Huang Hui Zhang Qing Lv kaori DSato Yan Qu Shitong Huan Jun Cheng Fei Zhao Lixia Wang National Center for TB control and prevention No 155 Changbai RoadChangping DistrictBeijing 102206China Duke Global Health Institute DurhamNCUSA Bill&Melinda Gates Foundation Beijing officeBeijingChina

Background:China’s national tuberculosis control program(NTP)provides free,first-line anti-tuberculosis(TB)drugs to pulmonary TB *** treatment regimen follows the World Health Organization’s(WHO)*** objective of this paper is to evaluate the current status of anti-TB drug use for newly diagnosed pulmonary TB inpatients treated in prefecture-and county-level designated ***:Three prefecture-level hospitals and nine county-level hospitals were selected for the *** newly diagnosed pulmonary TB inpatient medical records from 2012 were reviewed and doubly examined by two national senior *** rational use of anti-TB drugs was evaluated based on criteria in line with WHO’s ***:Of the 2,060 total treatment regimens for TB,53.1%were found to be rational(1093/2060).The percentages in prefecture-level and county-level hospitals were 50.3%(761/1513)and 60.7%(332/547),*** difference between the two levels of hospitals was statistically significant(Chi-square value=17.44,P<0.01).The percentages of rational treatment regimens for first-time hospitalizations and for two or more hospitalizations were 59.5%(983/1653)and 27.0%(110/407),respectively,with a statistically significant difference(Chi-square value=138.00,P<0.01).The overall use of second-line drugs(SLD)was 54.9%(1131/2060).The percentages for prefecture-level and county-level hospitals were 50.6%(766/1513)and 66.7%(365/547),respectively.A statistically significant difference was found(Chi-square value=42.06,P<0.01).The use of SLD for inpatients hospitalized once and inpatients hospitalized twice or more was 58.4%(966/1653)and 40.5%(165/407),respectively,with a statistically significant difference(Chi-square value=42.26,P<0.01).Conclusions:Half of inpatients might be treated with irrational regimens,and the use of SLD was more appropriately dispensed in city-level hospitals than in county-level *** and guidelines for health personnel,

关键词： Tuberculosis Inpatient Drug Regimen Rationality

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Pass Appearance Time and Pass Attempts by Teams Qualifying for the Second Stage of FIFA World Cup 2014 in Brazil

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Journal of Sports Science 2016年第3期4卷 156-162页

作者： kaori Saito Masahumi Yoshimura Department of Sports and Health Junior-High ·High School of Sakuragaoka Takinogawa Kita-ku 114-0023 Tokyo Japan Department of Health and Sport Sciences University of Juntendo Hiraga-gakuendai lnzai-shi 270-1695 Chiba Japan

This study aimed to clarify the difference between the not qualified teams and the qualified teams to 2nd stage in the Pass Appearance Time and passes attempted at all group stage games of World Cup 2014 and compared with World cup 2010. Results indicated changes in passing in modem football tactics. Actual playing time is represented as Net Playtime in this study. Pass Appearance Time is calculated by Net Playtime divided by passes Attempted. Pass Appearance Time of each team for the all Group stage games was calculated and compared with World cup 2010. No difference was revealed in the 2014 data between teams that did and did not qualify, though the 2010 data revealed differences. World cup 2010 data suggested that increasing passes attempts and lessening Pass Appearance Time were effective in qualifying for the 2rid stage. World cup 2014 data, however, suggested that diversity of tactics was important.

关键词： Pass Appearance Time passes attempts Net Playtime group stage games.

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Walking-friendly built environments and objectively measured physical function in older adults

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Journal of Sport and Health Science 2020年第6期9卷 651-656页

作者： Mohammad Javad Koohsari Gavin R.McCormack Tomoki Nakaya Ai Shibata kaori Ishii Akitomo Yasunaga Yung Liao Koichiro Oka Faculty of Sport Sciences Waseda UniversityTokorozawa 359-1192Japan Behavioural Epidemiology Laboratory Baker Heart and Diabetes InstituteMelbourneVIC 3004Australia Melbourne School of Population and Global Health The University of MelbourneMelbourneVIC 3010Australia Department of Community Health Sciences Cumming School of MedicineUniversity of CalgaryCalgaryAlberta T2N 4Z6Canada Faculty of Kinesiology University of CalgaryCalgaryAlberta T2N 4Z6Canada School of Architecture Planning and LandscapeUniversity of CalgaryCalgaryAlberta T2N 4Z6Canada Graduate School of Environmental Studies Tohoku UniversitySendai 980-0845Japan Faculty of Health and Sport Sciences University of TsukubaTsukuba 305-8574Japan Faculty of Liberal Arts and Sciences Bunka Gakuen UniversityTokyo 151-8523Japan

Background:Few studies have examined the associations between urban design attributes and older adults’physical ***,it is not well known how built-environment attributes may influence physical function in Asian *** aim of this study was to examine associations between objectively measured environmental attributes of walkability and objectively assessed physical function in a sample of Japanese older ***:Cross-sectional data collected in 2013 from 314 older residents(aged 65-84 years)living in Japan were *** function was estimated from objectively measured upper-and lower-body function,mobility,and balance by a trained research team member.A comprehensive list of built-environment attributes,including population density,availability of destinations,intersection density,and distance to the nearest public transport station,were objectively *** Score as a composite measure of neighborhood walkability was also ***:Among men,higher population density,availability of destinations,and intersection density were significantly associated with better physical function performance(1-legged stance with eyes open).Higher Walk Score was also marginally associated with better physical function performance(1-legged stance with eyes open).None of the environmental attributes were associated with physical function in elderly ***:Our findings indicate that environmental attributes of walkability are associated with the physical function of elderly men in the context of ***-friendly neighborhoods can not only promote older adults’active behaviors but can also support their physical function.

关键词： Elderly Functional test Neighborhood Urban design Walkability

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Use of the laser speckle flowgraphy in posterior fundus circulation research

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Chinese Medical Journal 2011年第24期124卷 4339-4344页

作者： ZHANG Wei Akiko Kogure kaori Yamamoto Sadao Hori Department ol Ophthalmology Tokyo Women＇s MedicalUniversity Tokyo 8-1 Kawada-cho Shinjuku-ku 162-8666Japan Department of Ophthalmology Nanjing First Hospital Affiliated to Nanjing Medical University Nanjing Jiangsu 210006 China

Objective To review articles aiming to present an overview of the principles, progress, uses and limitations of laser speckle flowgraphy （LSFG） in posterior fundus circulation research. Data sources The data used in this review was obtained mainly from the studies reported in PubMed using the key terms ＂laser speckle＂, ＂ocular blood flowmetry＂ and ＂retinal imaging＂. Study selection Relevant literatures on studies of LSFG were selected. Results LSFG is a unique, noninvasive imaging instrument to quantitatively visualize posterior fundus circulation in vivo This review delineates the LSFG principles and development, demonstrates its extensive applicability for measurement of retina, choroid and optic nerve head circulation, compares it with other retinal imaging technologies and discusses unresolved issues. Conclusions LSFG is a noninvasive, two-dimensional objective diagnostic technique that has become a powerful method for the clinical and scientific assessment of posterior fundus circulation. Further studies may help to develop a more comprehensive evidence-based measurement and facilitate the correlation with other methods for chorioretinal circulation assessment.

关键词： laser speckle blood flowmetry retina choroid optic nerve head

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Preparation of silymarin nanocrystals using a novel high pressure crystallization technique and evaluation of its dissolution and absorption properties

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Asian Journal of Pharmaceutical Sciences 2016年第1期11卷 211-212页

作者： Risako Onodera Tomohiro Hayashi Tatsuo Nakamura kaori Aibeb Kohei Tahara Hirofumi Takeuchi Gifu Pharmaceutical University 1-25-4 Daigaku-nishiGifu 501-1196Japan FANCL Corporation 12-13 Kamisinano Totsuka-kuYokohamaKanagawa 244-0806Japan

Oral bioavailability of drug is limited by the factors such as the membrane permeability, the solubility, the dissolution rate and so on (1)In case of a poorly water-soluble drug, its solubility and dissolution rate is a critical factor for its oral *** various techniques for enhancing solubility or dissolution properties, physical modification of drug products such as reducing the particle size is one of common approaches [2].

关键词： PureNano^(■) Silymarin Hydroxypropyl cyclodextrin Crystallization

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The isolation and identification of apolipoprotein C-I in hormone-refractory prostate cancer using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry

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Asian Journal of Andrology 2009年第3期11卷 299-307页

作者： kaori Yamamoto-Ishikawa Hiroyoshi Suzuki Masahiko Nezu Naoto Kamiya Takashi Imamoto Akira Komiya Kazuyuki Sogawa Takeshi Tomonaga Fumlo Nomur Tomohiko Ichikawa Department of Urology Graduate School of Medicine Chiba University Chiba 260-8670 Japan Department of Molecular Diagnosis Graduate School of Medicine Chiba University Chiba 260-8670 Japan

Androgens play a central role in prostate cancer pathogenesis, and hence most of the patients respond to androgen deprivation therapies. However, patients tend to relapse with aggressive prostate cancer, which has been termed as hormone refractory. To identify the proteins that mediate progression to the hormone-refractory state, we used protein-chip technology for mass profiling of patients＇ sera. This study included 16 patients with metastatic hormone-refractory prostate cancer who were initially treated with androgen deprivation therapy. Serum samples were collected from each patient at five time points： point A, pre-treatment; point B, at the nadir of the prostate- specific antigen （PSA） level; point C, PSA failure; point D, the early hormone-refractory phase; and point E, the late hormone-refractory phase. Using surface-enhanced laser desorption/ionization time-of-flight mass spectrometry, we performed protein mass profiling of the patients＇ sera and identified a 6 640-Da peak that increased with disease progression. Target proteins were partially purified, and by amino acid sequencing the peak was identified as a fragment of apolipoprotein C-I （ApoC-I）. Serum ApoC-I protein levels increased with disease progression. On immunohistochemical analysis, the ApoC-i protein was found localized to the cytoplasm of the hormone-refractory cancer cells. In this study, we showed an increase in serum ApoC-I protein levels in prostate cancer patients during their progression to the hormone-refractory state, which suggests that ApoC-I protein is related to progression of prostate cancer. However, as the exact role of ApoC-I in prostate cancer pathogenesis is unclear, further research is required.

关键词： apolipoprotein C-I hormonal therapy prognosis prostate cancer surface-enhanced laser desorption/ionization time-offlight mass spectrometry

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