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Chromosomal Location of a Recessive Red-Eye Mutant Gene in the Brown Planthopper <i>Nilaparvata lugens</i>(Stål) (Insecta: Hemiptera)

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Advances in Entomology 2017年第1期5卷 33-39页

作者： Jirapong Jairin Phikul Leelagud Adisak Pongmee kanuengnij srivilai Ubon Ratchathani Rice Research Center Ubon Ratchathani Thailand Roi Et Agricultural Research and Development Center Roi Et Thailand Sakon Nakhon Rice Research Center Sakon Nakhon Thailand

The color of compound eyes is an important biological characteristic of insects. A red eye color mutation is commonly found in the brown planthopper (BPH), Nilaparvata lugens (Stål) (Hemiptera: Delphacidae), a serious insect pest of rice in tropical and temperate Asia. The genetic inheritance and physiological effect of the eye color mutation in the BPH have been studied, but the location of a red gene controlling the red eye mutant phenotype on a chromosome has not been elucidated. In this study, simple sequence repeats (SSRs), together with bulked segregant analysis (BSA), was performed to identify and map the location of the red gene. A total of 387 SSR markers distributed throughout the BPH autosome were used to survey two bulked DNA samples. Samples were generated from 29 brown-eyed and 29 red-eyed individuals derived from an F2 generation of a cross between brown-eyed wild type and red-eyed mutant colonies. The SSR marker BM20 was shown to be associated with the red eye mutant phenotype. Ninety-five offspring of the F2 generation were then used to map the gene. The present study constitutes the discovery of the location of the red gene, which may lead to the acquisition of the genetic determinant of the compound eye color mutation in BPH.

关键词： Brown Planthopper Bulked Segregant Analysis Linkage Map Red-Eyed Mutant Simple Sequence Repeat

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Identification of QTLs for Blast, Bacterial Blight, and Planthopper Resistance Using SNP-Based Linkage Maps from Two Recombinant Inbred Rice Lines

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American Journal of Plant Sciences 2019年第5期10卷 760-779页

作者： Jirapong Jairin Phanchita Vejchasarn Thanapa Somjai kanuengnij srivilai Kulchana Darwell Phikul Leelagud Rungnapa Kawichai Jate Kotcharerk Arissara Suthanthangjai Nattaya Popa Suphalaksana Lachanthuek Varapong Chamarerk Ubon Ratchathani Rice Research Center Mueang Ubon Ratchathani Thailand Sakon Nakhon Rice Research Center Mueang Sakon Nakhon Thailand Phrae Rice Research Center Mueang Phrae Thailand Phitsanulok Rice Research Center Wang Thong Phitsanulok Thailand GenePlus Co. Ltd. Huai Khwang Bangkok Thailand Rice Department Chatuchak Bangkok Thailand

Rice is the most significant global food security. Several biotic factors limit rice production, breeding biotic-resistant rice has, therefore, become an increasingly important goal. Two elite rice lines, IR71033-121-15 (IR71033) and IR57514-PMI-5-B-1-2 (IR57514), provide potential genes for biotic stress resistance traits. In this study, genotyping by sequencing (GBS) for single nucleotide polymorphism (SNP)-based linkage map construction was used to detect quantitative trait loci (QTLs) for blast (BL), bacterial blight (BB), whitebacked planthopper (WBPH), and brown planthopper (BPH) resistance. IR71033 was derived from Oryza minuta and carried BL, BB, WBPH, and BPH resistance QTLs. IR57514 is a well-adapted rainfed lowland line that carries BL and BB resistance QTLs. Two sets of recombinant inbred line (RIL) populations derived from crosses of KDML105 × IR71033 and KDML105 × IR57514 were used to dissect the genetic basis of disease and insect pest resistance. The RIL populations were evaluated for BL, BB, WBPH, and BPH resistance from 2016 to 2018 at four rice research centers in Thailand. From these, we identified a large number of SNPs through GBS and constructed high-resolution linkage maps. By combining phenotypic evaluation with the GBS data, a total of 24 QTLs on four chromosomes were detected that confered pest resistance and explained 7.3% - 61.4% of the phenotypic variance. These findings should facilitate identifying novel resistance genes and applying marker-assisted selection for resistance to the four major rice pests investigated here. These strategies will improve the resilience and reliability of rice varieties adapted to the low-yielding environment of rainfed lowland areas worldwide.

关键词： Recombinant Inbred Line Genotyping by Sequencing Resistance Gene Rainfed Lowland Rice Single Nucleotide Polymorphism Quantitative Trait Locus

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Skin Absorption Enhancing Properties of Germacrone and Curcuma aeruginosa Extract on Minoxidil

Skin Absorption Enhancing Properties of Germacrone and Curcu...

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第十四届全国中药和天然药物学术研讨会

作者： Jukkarin srivilai Neti Waranuch Nantaka Khorana Wudtichai Wisutthiprot Anothai Tangsamranjit Kornkanok Ingkaninan Naresuan University PhitsanulokThailand

＜正＞Androgenic alopecia is the most common form of hair *** has tremendous negative impact on a person’s self-respect,and overall quality of *** solution of minoxidil is used for treatment this diseases but it has limitation with poor skin absorption *** of the attempts to improve its therapeutic efficacy is to combine minoxidil with skin penetration

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