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Identification,Characterization,and Probiotic Potentials of Lactobacillus pentosus SF-1

Journal of Ocean University of China 2024年第2期23卷 509-517页

作者： JIAO Lin LIANG Shufei CHU Ruixia WANG Wentao mai kangsen LIU Ming WAN Min Key Laboratory of Aquaculture Nutrition and Feed Ministry of Agriculture&Key Laboratory of MaricultureMinistry of EducationCollege of FisheriesOcean University of ChinaQingdao 266003China Engineering Research Center of Mariculture Ministry of EducationOcean University of ChinaQingdao 266003China

In recent years probiotics have been considered as a potential substitution of antibiotics to control pathogens and treat infectious diseases in aquaculture.In the present study a strain of Lactobacillus pentosus,named as L.pentosus SF-1,was isolated from waters in aquaculture.The species identification of this strain was conducted by 16S rRNA sequence,and the physiological and biochemical characteristics of this strain were assessed.Furthermore,the virulence,antibiotic sensitivity,cell surface characteristics and acid/base-resistance of L.pentosus SF-1 were determined to evaluate the probiotic potentials of this strain.Specifically,L.pentosus SF-1 is sensitive to most common antibiotics,and no hemolysin was generated from it,indicating the safety of this strain to hosts.In addition,L.pentosus SF-1 was able to tolerate the artificial gastric juice at pH 3 for 4 h and the artificial intestinal fluid at pH 6.8 or 8.0 for 6 h.Moreover,the analysis of self-aggregation and the adhesion of L.pentosus SF-1 to organic solvents suggested a high potential of L.pentosus SF-1 to inhabit the hosts,which was confirmed by testing the colonization of L.pentosus SF-1 in germ-free zebrafish.Interestingly,L.pentosus SF-1 displayed a high bactericidal activity against several bacterial pathogens.Consistently,the incubation of L.pentosus SF-1 significantly promoted the expression of antimicrobial components in zebrafish,contributing to the protection of the fish from E.tarda infection in vivo.Taken together,the probiotic strain L.pentosus SF-1 could be applied as anti-infection reagent in aquaculture.

关键词： Lactobacillus pentosus probiotics antibiotics hemolysis cell surface characteristics bactericidal activity infection

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Dietary xanthophyll improved growth,antioxidant,pigmentation and meat quality in the southern catfish(Silurus soldatovi meridionalis Chen)

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Animal Nutrition 2023年第2期13卷 101-115页

作者： Chaoqun He Haoyu Geng Yawen Qin Peng Yang Wenqiang Wang kangsen mai Fei Song Institute of Modern Aquaculture Science and Engineering(IMASE) Guangzhou Key Laboratory of Subtropical Biodiversity and BiomonitoringGuangdong Provincial Key Laboratory for Healthy and Safe AquacultureCollege of Life ScienceSouth China Normal UniversityGuangzhou510631China Southern Marine Science and Engineering Guangdong Laboratory(Zhuhai) Zhuhai519000China

Xanthophyll has multiple physiological functions to improve the quality of farmed animals.The present study aimed to investigate the effects of xanthophyll on the growth performance,antioxidation,immunity,pigmentation and meat quality of southern catfish(Silurus soldatovi meridionalis Chen).Juvenile southern catfish(18.35±0.04 g)were randomly allocated into 24 cages(30 juveniles per cage),and fed diets with different dietary xanthophyll levels(at 14,42,80,108,126 and 152 mg/kg,dry matter of diet)twice daily for 8 weeks.Results indicated that the diet with 80 mg/kg xanthophyll induced a higher specific growth rate(SGR),feed efficiency ratio and protein productive value.Moreover,the 80 mg/kg xanthophyll diet also increased complement 3,immunoglobulin M(IgM)and lysozyme content more than the other groups.The mRNA expression level of inflammation-related genes and antioxidant enzyme activities further confirmed the effects of 80 mg/kg dietary xanthophyll on improving immune response.The present study also found that the 126 mg/kg xanthophyll diet significantly enhanced the content of total carotenoids and xanthophyll,hydroxyproline,collagen and amino acid in muscle.The diet with 126 mg/kg xanthophylls also induced lower drip loss,thawing loss,centrifugal loss,cooking loss and higher muscle adhesiveness,cohesiveness,springiness,gumminess and chewiness than the other treatments.In conclusion,quadratic regression model analysis based on SGR and IgM revealed that the optimum xanthophyll level in the diet was 86.78 and 84.63 mg/kg,respectively.Moreover,broken line regression analysis based on xanthophyll content in dorsal skin and chewiness in muscle demonstrated that the optimal xanthophyll level was between 89.73 and 108.66 mg/kg in the diet of southern catfish(S.soldatovi meridionalis Chen).

关键词： Xanthophyll Growth performance Antioxidant capacity Fish quality Southern catfish(Silurus soldatovi meridionalis Chen)

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Recent progress in the understanding of the gut microbiota of marine fishes

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Marine Life Science & Technology 2021年第4期3卷 434-448页

作者： Weihao Ou Guijuan Yu Yanjiao Zhang kangsen mai The Key Laboratory of Aquaculture Nutrition and Feed(Ministry of Agriculture)and the Key Laboratory of Mariculture(Ministry of Education) Ocean University of ChinaQingdao 266003China Laboratory for Marine Fisheries Science and Food Production Processes Pilot National Laboratory for Marine Science and TechnologyQingdao 266237China

As the significance of the gut microbiota has become increasingly realized,a large number of related studies have emerged.With respect to the gut microbial composition of fish,the predominant gut microbes and core gut microbiota have been reported by many researchers.Our understanding of fish gut microbiota,especially its functional roles,has fallen far behind that of terrestrial vertebrates,although previous studies using gnotobiotic zebrafish models have revealed that the gut microbiota performs a significant role in gut development,nutrient metabolism and immune responses.Given that environmental factors of marine habitats are very different from those of freshwater habitats,a distinct difference may exist in the gut microbiota between freshwater and marine fish.Therefore,this review aims to address the advances in marine fish gut microbiota in terms of methodologies,the gut microbial composition,and gnotobiotic models of marine fish,the important factors(host genotype and three environmental factors:temperature,salinity and diet)that drive marine fish gut microbiota,and significant roles of the gut microbiota in marine fish.

关键词： Marine fish Gut microbiota Gnotobiotic Influence factors Gut microbial functions

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The Fish Microbiota:Research Progress and Potential Applications

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Engineering 2023年第10期29卷 137-146页

作者： Yinyin Luan Ming Li Wei Zhou Yuanyuan Yao Yalin Yang Zhen Zhang Einar Ringø Rolf Erik Olsen Jihong Liu Clarke Shouqi Xie kangsen mai Chao Ran Zhigang Zhou China–Norway Joint Lab on Fish Gastrointestinal Microbiota Institute of Feed ResearchChinese Academy of Agricultural SciencesBeijing 100081China Biotechnology of the Ministry of Agriculture and Rural Affairs Institute of Feed ResearchChinese Academy of Agricultural SciencesBeijing 100081China Norway–China Joint Lab on Fish Gastrointestinal Microbiota Institute of BiologyNorwegian University of Science and TechnologyTrondheim 7491Norway NIBIO Norwegian Institute of Bioeconomy ResearchÅs 1431Norway State Key Laboratory of Freshwater Ecology and Biotechnology Institute of HydrobiologyChinese Academy of SciencesWuhan 430072China University of Chinese Academy of Sciences Beijing 100049China The Innovative Academy of Seed Design Chinese Academy of SciencesBeijing 100101China Hubei Engineering Research Center for Aquatic Animal Nutrition and Feed Wuhan 430072China The Key Laboratory of Mariculture(Ministry of Education) Ocean University of ChinaQingdao 266003China

The gut microbiota plays an important role in host health and disease.Our understanding of the fish microbiota lags far behind our knowledge of that of humans and other mammals.Nevertheless,research has highlighted the importance of the microbiota in the health,performance,and various physiological functions of fish.The microbiota has been studied in various fish species,including model animals,economic fish,and wild fish species.The composition of the fish microbiota depends on host selection,diet,and environmental factors.The intestinal microbiota affects the nutritional metabolism,immunity,and disease resistance of the fish host,while the host regulates the intestinal microbiota in a reciprocal way through both immune and non-immune factors.Improved and novel gnotobiotic fish models have been developed,which are important for the mechanistic study of host–microbiota interactions in fish.In this review,we discuss recent progress in fish microbiota research.We describe various aspects of this research,including both studies on fish microbiota variations and fundamental research extending our knowledge of host–microbiota interaction in fish.Perspectives on how fish microbiota research may benefit fish health and industrial sustainability are also discussed.

关键词： Gut microbiota Fish Host-microbiota interaction Aquaculture

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Recent advances in amino acid sensing and new challenges for protein nutrition in aquaculture

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Marine Life Science & Technology 2019年第1期1卷 50-59页

作者： Chengdong Liu Xuan Wang Huihui Zhou kangsen mai Gen He Key Laboratory of Mariculture Ministry of EducationOcean University of China5 Yushan RoadQingdao 266003China Laboratory for Marine Fisheries Science and Food Production Processes Qingdao National Laboratory for Marine Science and TechnologyQingdao 266237China

From the conventional knowledge of protein nutrition to the molecular nutrition of amino acids, our understanding of protein/amino acid nutrition is rapidly increasing. Amino acids control cell growth and metabolism through two amino acid-sensingpathways, i.e. target of rapamycin complex 1 (TORC1) and the general control nonderepressible 2 (GCN2) signaling pathway.In the amino acid-abundant status, TORC1 dominates intracellular signaling and increases protein synthesis and cell growth.In contrast, amino acid deprivation actives GCN2 resulting in repression of general protein synthesis but facilitates the aminoacid transport and synthesis process. By integrating and coordinating nutrition and hormone signaling, TORC1 and GCN2control the switch of the catabolism and anabolism phase in most eukaryotes. Now, we appreciate that the availability ofindividual amino acids is sensed by intracellular sensors. These cutting-edge findings expand our knowledge of amino acidnutrition. Although the TORC1 and GCN2 were discovered decades ago, the study of molecular amino acid nutrition inaquaculture animals is still at its infancy. The aquaculture industry is highly dependent on the supply of fishmeal, which isthe major protein source in aquacultural animal diets. Some concerted efforts were conducted to substitute for fishmeal dueto limited supply of it. However, the concomitant issues including the unbalanced amino acid profile of alternative proteinsources limited the utilization of those proteins. Continued study of the molecular nutrition of amino acid in aquacultureanimals may be expected in the immediate future to expand our knowledge on the utilization of alternative protein sources.

关键词： Molecular nutrition Amino acid sensor GCN2 TORC1 Aquaculture Fishmeal replacement

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Roles of dietary taurine in fish nutrition

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Marine Life Science & Technology 2020年第4期2卷 360-375页

作者： W.W.H.A.Sampath R.M.D.S.Rathnayake Mengxi Yang Wenbing Zhang kangsen mai The Key Laboratory of Aquaculture Nutrition and Feeds Ministry of Agriculture and Rural AffairsQingdao 266003China The Key Laboratory of Mariculture Ministry of EducationQingdao 266003China Ocean University of China Qingdao 266003.China Developmental Molecular Biology Laboratory Ocean University of ChinaQingdao 266003China Laboratory for Marine Fisheries Science and Food Production Process Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266237China

Taurine is a conditionally essential amino acid in fish nutrition.The present study addressed the practical application of examining published data on fish nutrition over the past 20 years,emphasizing the topic of taurine by using computational tools and their applications.According to the published articles,an increased linear growth of research occurred,with Japanese flounder being the most examined fish species.Dietary taurine supplementation has several beneficial effects in fish nutrition,such as survival,growth,feed utilization,protein and energy retention,intermediate metabolism,anti-oxidation,anti-stress,disease resistance,muscle texture and reproductive performance.Also,there are negative effects in some species.Dietary taurine exerted effects on several gene expressions and enzyme activities;these are important in taurine metabolism in fish.These genes and enzymes included taurine transporter(TauT),cysteine dioxygenase(CDO),cysteamine dioxygenase(ADO),cysteine sulfonate decarboxylase(CSD)and pretrypsinogen(Ptry).Plant protein-based diets with taurine supplementation are recommended because of the absence of taurine in plani protein.

关键词： Taurine Fish Amino acid Nutrition Feed

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脂肪酸受体GPR119在动物脂代谢调控中的作用研究进展

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中国海洋大学学报（自然科学版） 2024年第7期54卷 123-129页

作者：艾庆辉刘国滨卜宪勇王震麦康森中国海洋大学农业农村部水产动物营养与饲料重点实验室山东青岛266003 海水养殖教育部重点实验室(中国海洋大学) 山东青岛266003

中国是水产养殖大国,而高脂饲料的广泛使用导致了鱼类脂肪代谢异常,这严重制约了水产养殖业的发展,亟待对此问题寻求解决方案。近年来在哺乳动物中的研究表明,脂代谢紊乱诱导的非酒精性脂肪肝病(Non-alcoholic fatty liver disease, NAF... 详细信息

中国是水产养殖大国,而高脂饲料的广泛使用导致了鱼类脂肪代谢异常,这严重制约了水产养殖业的发展,亟待对此问题寻求解决方案。近年来在哺乳动物中的研究表明,脂代谢紊乱诱导的非酒精性脂肪肝病(Non-alcoholic fatty liver disease, NAFLD)是危害动物健康的主要问题之一,而GPR119被认为是治疗NAFLD的新靶点。GPR119在鱼类中的研究相对较少,大部分鱼类GPR119功能相关研究主要聚焦于其在调控鱼类食欲中的作用,而其在调控鱼类脂肪代谢中的作用尚不清楚。本文总结了动物中GPR119基因的结构功能及其对脂肪代谢调控机制的研究现状,旨在为研究GPR119在鱼类脂肪代谢中的作用提供新思路,为探索缓解鱼类脂代谢紊乱的新型策略提供理论参考。

关键词： GPR119 脂肪异常沉积脂代谢鱼类

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低蛋白饲料添加亮氨酸、精氨酸对大菱鲆幼鱼生长、消化、免疫及mTOR信号通路的影响

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中国海洋大学学报（自然科学版） 2024年第5期54卷 33-43页

作者：田原刘成栋王旋周慧慧麦康森何艮海水养殖教育部重点实验室(中国海洋大学) 山东青岛266003

为探究亮氨酸和精氨酸作为信号分子参与调控mTOR信号通路对大菱鲆(Scophthalmus maximus)生长的作用,本研究以大菱鲆幼鱼(初始体质量(13.50±0.19)g)为实验对象,设置50%蛋白水平饲料作为阳性对照组,45%蛋白水平饲料作为阴性对照组,设置... 详细信息

为探究亮氨酸和精氨酸作为信号分子参与调控mTOR信号通路对大菱鲆(Scophthalmus maximus)生长的作用,本研究以大菱鲆幼鱼(初始体质量(13.50±0.19)g)为实验对象,设置50%蛋白水平饲料作为阳性对照组,45%蛋白水平饲料作为阴性对照组,设置两个实验组,分别为在阴性对照组中添加1%亮氨酸的实验组和添加1%精氨酸的实验组,饲养周期为56 d,测量大菱鲆的生长性能和饲料利用水平。研究表明,添加亮氨酸、精氨酸均能一定程度提高因饲料蛋白水平不足导致的大菱鲆的特定生长率、蛋白质效率和增重率下降。添加1%亮氨酸和1%精氨酸均能在摄食后有效增强大菱鲆肌肉与肝脏mTOR信号通路中的雷帕霉素靶蛋白(mTOR)、核糖体蛋白S6和真核起始因子4E结合蛋白1(4E-BP1)的磷酸化。添加1%精氨酸还能够提高大菱鲆肠道淀粉酶和脂肪酶活性,显著提高血清过氧化氢酶和溶菌酶水平并增加血清总抗氧化能力。研究结果表明,在低蛋白饲料中添加1%亮氨酸、1%精氨酸能够激活大菱鲆mTOR信号通路,有效提高其生长性能和蛋白质效率;此外,添加1%精氨酸还能够提高大菱鲆的消化酶活性、增强非特异性免疫反应。

关键词：大菱鲆亮氨酸精氨酸 mTOR信号通路低蛋白饲料

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脱酚棉籽蛋白替代豆粕对凡纳滨对虾生长、饲料利用和免疫应答的影响

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中国海洋大学学报（自然科学版） 2024年第6期54卷 45-54页

作者：李洁王旋周慧慧刘成栋麦康森何艮海水养殖教育部重点实验室(中国海洋大学) 山东青岛266003

为研究脱酚棉籽蛋白替代凡纳滨对虾(Litopenaeus vannamei)饲料中豆粕的效果,以初始体质量为(0.81±0.01) g的凡纳滨对虾为研究对象,进行6周的养殖实验。对照组饲料中豆粕含量为25%(编号为S0),分别用4.93%、10.06%、14.80%、19.73%的脱... 详细信息

为研究脱酚棉籽蛋白替代凡纳滨对虾(Litopenaeus vannamei)饲料中豆粕的效果,以初始体质量为(0.81±0.01) g的凡纳滨对虾为研究对象,进行6周的养殖实验。对照组饲料中豆粕含量为25%(编号为S0),分别用4.93%、10.06%、14.80%、19.73%的脱酚棉籽蛋白等蛋白替代25%、50%、75%、100%的豆粕(分别编号为S25, S50, S75, S100)。每个处理3个重复,每个重复70尾对虾。实验表明,与对照组相比,脱酚棉籽蛋白替代100%豆粕对对虾的生长和饲料利用无显著影响,但显著降低了胰蛋白酶和淀粉酶活性,显著降低了干物质和粗蛋白表观消化率。与对照组相比,脱酚棉籽蛋白替代75%豆粕时,对虾肠道中免疫相关基因的表达显著升高。肠道组织形态学观察实验表明,脱酚棉籽蛋白替代100%豆粕会影响对虾的肠道健康,肠道绒毛高度显著降低。结果表明,脱酚棉籽蛋白替代饲料中100%的豆粕不会对凡纳滨对虾的生长和饲料利用产生显著影响,但会影响对虾的消化、免疫应答以及肠道健康。

关键词：凡纳滨对虾脱酚棉籽蛋白豆粕饲料利用生长免疫应答

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Optimal amounts of coconut oil in diets improve the growth,antioxidant capacity and lipid metabolism of large yellow croaker(Larimichthys crocea)

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Marine Life Science & Technology 2020年第4期2卷 376-385页

作者： Tao Ding Ning Xu Yongtao Liu Xueshan Li Xiaojun Xiang Dan Xu Chuanwei Yao Qiangde Liu Zhaoyang Yin kangsen mai Qinghui Ai Key Laboratory of Aquaculture Nutrition and Feed Ministry of Agriculture and Rural Affairsand Key Laboratory of MaricultureMinistry of EducationOcean University of China.Qingdao 266003China Laboratory for Marine Fisheries Science and Food Production Processes Qingdao National Laboratory for Marine Science and TechnologyQingdao 266237China

The purpose of this study was to investigate the effect of different dietary coconut oil(CO)levels on growth,antioxidant capacity and lipid metabolism of juvenile large yellow croaker(Larimichthys crocea).Five iso-nitrogen(45%crude protein)and iso-lipid(13%crude lipid)experimental diets were prepared by replacing 0%(the control),25%,50%,75%and 100%fish oil with coconut oil.The results showed that dietary CO had no significant effect on survival rate(SR.P>0.05).However,the specific growth rate was increased significantly when compared with the control group when fish were fed the diet with 50%CO(P<0.05).The saturated fatty acids were increased significantly with increasing dietary CO in the liver and muscle,whereas the content of n-3 PUFA was decreased significantly(P<0.05).The highest activities of glutathione peroxidase and superoxide dismutase in the liver were recorded in fish-fed diet with 50%CO;conversely,the content of malondialdehyde was significantly decreased(P<0.05).The mRNA expression of peroxisome proliferator-activated receptor a,carnitine palmitoyl transferase 1 and acyl-CoA oxidase reached the highest levels in fish-fed diet with 50%CO.To some extent,this indicated that the rapid oxidation reaction of fatty acids to provide energy may be the reason for the rapid growth of large yellow croaker.In conclusion,fish-fed diet with 50%CO increased the growth rate and antioxidant capacity.Therefore,the optimal replacement level of CO to FO in the diet should be 50%.

关键词： Coconut oil Growth performance Antioxidant capacity Lipid metabolism Larimichthys crocea

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