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m.lecular characterization of DnaJ 5 hom.logs in silkworm.Bom.yx m.ri and its expression during egg diapause

Insect Science 2014年第6期21卷 677-686页

作者： Sasibhushan Sirigineedi Esvaran Vijayagowri Geetha N. m.rthy Guruprasada Rao kangayam m. ponnuvel Genom.cs Division Seribiotech Research Laboratory Carmelaram Post Kodathi Bangalore 560035 Karnataka India

A com.arison of the cDNA sequences （1 056 bp） of Bom.yx m.ri DnaJ 5 hom.log with B. m.ri genom. revealed that unlike in other Hsps, it has an intron of 234 bp. The DnaJ 5 hom.log contains 351 am.no acids, of which 70 contain the conserved DnaJ dom.in at the N-term.nal end. This hom.log orB. m.ri has all desirable functional dom.ins sim.lar to other insects, and the 13 different DnaJ hom.logs identified in B. m.ri genom. were distributed on different chrom.som.s. The expressed sequence tag database analysis of Hsp40 gene expression revealed higher expression in wing disc followed by diapause-induced eggs. m.croarray analysis revealed higher expression of DnaJ 5 hom.log at 18th h after oviposition in diapause-induced eggs. Further validation of DnaJ 5 expression through qPCR in diapause-induced and nondiapause eggs at different tim. intervals revealed higher expression in diapause eggs at 18 and 24 h after oviposition, which coincided with the expression of Hsp70 as the Hsp 40 is its co-chaperone. This study thus provides an outline of the genom. organization of lisp40 gene, and its role in egg diapause induction in B. m.ri.

关键词： Bom.yx m.ri diapause DnaJ exon Hsp40 intron

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Analysis of genetic relationship in m.tant silkworm.strains of Bom.byx m.ri using inter sim.le sequence repeat (ISSR) m.rkers

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Journal of Genetics and Genom.cs 2008年第5期35卷 291-297页

作者： Dhanikachalam.Velu kangayam m. ponnuvel m.rugiah m.thulakshm. Randhir K. Sinha Syed m.H. Qadri Biotechnology Laboratory Central Sericultural Germplasm Resources Centre Hosur-635109 Tamil Nadu India

Am.lified inter sim.le sequence repeats （ISSR） m.rkers were used to determ.ne genetic relationships am.ng m.tant silkworm.strains of Bom.yx m.ri. Fifteen ISSR prim.rs containing sim.le sequence repeat （SSR） m.tifs were used in this study. A total of 113 m.rkers were produced am.ng 20 m.tant strains, of which 73.45% were found to be polym.rphic. In selected m.tant genetic stocks, the average num.er of observed allele was （1.7080 ± 0.4567）, effective alleles （1.5194 ± 0.3950） and genetic diversity （Ht）（0.2901 ± 0.0415）. The dendrogram.produced using the unweighted pair group m.thod with arithm.tic m.ans （UPGm.） and cluster analysis m.de using Nei＇s genetic distance resulted in the form.tion of one m.jor group containing 6 groups separated 20 m.tant silkworm.strains. Therefore, ISSR am.lification is a valuable m.thod for determ.ning the genetic variability am.ng m.tant silkworm.strains. This efficient m.lecular m.rker would be useful for characterizing a considerable num.er of silkworm.strains m.intained at the germ.lasm.center.

关键词： genetic diversity Bom.yx m.ri m.tant strains ISSR

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Analysis of bulked segregants to identify m.lecular m.rkers linked with cocoon weight and cocoon shell weight in the silkworm.Bom.yx m.ri L

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Journal of Zhejiang University Science 2002年第3期3卷 348-354页

作者： SateeshKum.r 徐孟奎陈玉银 ponnuvel,K.m.Datta,R.K Departm.ntofSericulture&Apiculture ZhejiangUniversityHangzhou310029.China m.lecularBiologyLaboratory CentralSerlculturalResearchandTrainingInstituteMysore570008India

Two silkworm.strains viz, B20 A (high cocoon shell ratio) and *** (low cocoon shell ratio) were sib m.ted for 10 generations to determ.ne the hom.zygosis. Both bulked segregant analysis(BSA) and near isogenic lines (NIL) studies were done to identify the RFLP m.rkers closely linked to cocoon shell param.ters. Three hundred and fifty two random.clones were identified as the low copy num.er sequence and used for identification of Restriction Fragm.nt Length Polym.rphic (RFLP) m.rker linked to cocoon weight and cocoon shell character. In the bulk segregant analysis, DNA from.the parents (B20 A, ***), F 1 and F 2 progeny of high shell ratio (HSR) and low shell ratio (LSR) were screened for hybridization with the random.clones. Polym.rphic banding pattern achieved through southern hybridization with different probes indicated the probable correlation of polym.rphism.with high and low cocoon shell character which are possible landm.rks in identifying the putative m.rker(s) for the cocoon shell character. Out of the 100 probes tried with parents, F 1, F 2 and their bulks, 10 probes were found to be closely linked to cocoon shell characters.

关键词： Restriction fragm.nt length polym.rphic (RFLP) m.lecular m.rker Bom.yx m.ri L Shell ratio Bulked segregant analysis(BSA) Near isogenic lines

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