检索结果-南通市图书馆

Synthesis of PEGylated hyaluronic acid for loading dichloro(1,2-diaminocyclohexane)platinum(Ⅱ)(DACHPt) in nanoparticles for cancer treatment

引用

Chinese Chemical Letters 2015年第6期26卷 695-699页

作者： Xiu-quan quan Lin kang Xue-Zhe yin Zhe-Hu Jin Zhong-Gao Gao State Key Laboratory of Bioactive Substance and Function of Natural Medicines Institute of Materia MedicaChinese Academy of Medical Sciences and Peking Union Medical College Yanbian University Hospital

Dichloro（1,2-diaminocyclohexane）platinum（ll）（DACHPt）, a cisplatin （CDDP） analog, has shown lower toxicity than CDDP and no cross-resistance with CDDP in many CDDP-resistant cancers. PEGylated hyaluronan （mPEG-HA） is an mPEG conjugated with hyaluronan biodegradable polymer which is a naturally occurring biopolymer in the interstitium, is primarily cleared by the lymphatic system, mPEG- hyaluronan-DACHPt （PEG-HA-Pt） conjugate could circulate long-term in the bloodstream and increase DACHPt concentration in the tumor site and decrease systemic toxicity, mPEG-HA conjugates with the range of 1%-5% substitution were synthesized, and the structures were confirmed by 1H NMR and IR. The particle size of DACHPt incorporated with mPEG-HA was about 86 nm and the loading content and efficiency were about 19% （w/w） and 86%, respectively. The synthesized mPEG-HA with different PEG substitution degrees presented non toxicity, and the cell viability of DACHPt loaded in mPEG-HA nanoparticles increased with increasing doses of DACHPt. DACHPt release from nanoparticles slightly decreased with increasing PEG substitution degree from 1% to 5% at 37℃, pH 7.4 PBS solution. The DACHPt loaded in mPEG-HA nanoparticles significantly inhibited the growth ofA549 xenografts in nude mice when compared to the DACHPt loaded in HA nanoparticles and the control group after 4 weeks treatment （p 〈 0.01 compared with control）. The body weight change curve shows that the mice weight loss was less than 5% by treating with both DACHPt loaded in mPEG-HA and HA nanoparticles. In conclusion, a novel DACHPt loaded mPEG-HA delivery system was developed with sustained release and increased platinum concentration in the tumor.

关键词： DACHPt PEGylated hyaluronan A549 lung cancer cell

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Inhibition of LncRNA Vof-16 expression promotes nerve regeneration and functional recovery after spinal cord injury

引用

Neural Regeneration Research 2022年第1期17卷 217-227页

作者： Xiao-Min Zhang Li-Ni Zeng Wan-Yong Yang Lu Ding kang-Zhen Chen Wen-Jin Fu Si-quan Zeng yin-Ru Liang Gan-Hai Chen Hong-Fu Wu Key Laboratory of Stem Cell and Regenerative Tissue Engineering Guangdong Medical UniversityDongguanGuangdong ProvinceChina Biology Research Group Guangzheng Experimental SchoolHuizhouGuangdong ProvinceChina Geriatric Medicine Center Dongguan Waterfront Zone Central HospitalDongguanGuangdong ProvinceChina Scientific Research Center the Seventh Affiliated HospitalSun Yat-sen UniversityShenzhenGuangdong ProvinceChina Clinical Laboratory Affiliated Houjie HospitalGuangdong Medical UniversityDongguanGuangdong ProvinceChina Department of Intensive Care Unit Affiliated Houjie HospitalGuangdong Medical UniversityDongguanGuangdong ProvinceChina

Our previous RNA sequencing study showed that the long non-coding RNA ischemia-related factor Vof-16(lncRNA Vof-16)was upregulated after spinal cord injury,but its precise role in spinal cord injury remains unclear.Bioinformatics predictions have indicated that lncRNA Vof-16 may participate in the pathophysiological processes of inflammation and apoptosis.PC12 cells were transfected with a pHBLV-U6-MCS-CMV-ZsGreen-PGK-PURO vector to express an lncRNA Vof-16 knockdown lentivirus and a pHLV-CMVIE-ZsGree-Puro vector to express an lncRNA Vof-16 overexpression lentivirus.The overexpression of lncRNA Vof-16 inhibited PC12 cell survival,proliferation,migration,and neurite extension,whereas lncRNA Vof-16 knockdown lentiviral vector resulted in the opposite effects in PC12 cells.Western blot assay results showed that the overexpression of lncRNA Vof-16 increased the protein expression levels of interleukin 6,tumor necrosis factor-α,and Caspase-3 and decreased Bcl-2 expression levels in PC12 cells.Furthermore,we established rat models of spinal cord injury using the complete transection at T10.Spinal cord injury model rats were injected with the lncRNA Vof-16 knockdown or overexpression lentiviral vectors immediately after injury.At 7 days after spinal cord injury,rats treated with lncRNA Vof-16 knockdown displayed increased neuronal survival and enhanced axonal extension.At 8 weeks after spinal cord injury,rats treated with the lncRNA Vof-16 knockdown lentiviral vector displayed improved neurological function in the hind limb.Notably,lncRNA Vof-16 knockdown injection increased Bcl-2 expression and decreased tumor necrosis factor-αand Caspase-3 expression in treated animals.Rats treated with the lncRNA Vof-16 overexpression lentiviral vector displayed opposite trends.These findings suggested that lncRNA Vof-16 is associated with the regulation of inflammation and apoptosis.The inhibition of lncRNA Vof-16 may be useful for promoting nerve regeneration and functional recovery after spinal cord injury.The experiments were app

关键词： apoptosis functional recovery inflammation long non-coding RNA ischemia related factor Vof-16 nerve regeneration nerve repair neurite extension neuronal survival proliferation spinal cord injury

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Microvolt T-wave alternans complemented with electrophysiologic study for prediction of ventricular tachyarrhythmias in patients with arrhythmogenic right ventricular cardiomyopathy: a long-term follow-up study

引用

Chinese Medical Journal 2019年第12期132卷 1406-1413页

作者： She-Liang Xue Xiao-Feng Hou kang-Yun Sun Yao Wang Zhi-Yong Qian quan-Peng Wang Si-Peng Shen Hong-Li yin Rong Zhang Hai-Ping yin Jian-Gang Zou Department of Cardiology The First Affiliated Hospital of Nanjing Medical UniversityNanjingJiangsu 210029China Department of Cardiology The Affiliated Wujin Hospital of Jiangsu UniversityChangzhouJiangsu 213002China Department of Cardiology The Affiliated Suzhou Hospital of Nanjing Medical UniversitySuzhouJiangsu 215008China Department of Biostatistics School of Public HealthNanjing Medical UniversityNanjingJiangsu 211166China Key Laboratory of Targeted Intervention in Cardiovascular Disease Collaborative Inn ovation Center for Cardiovascular Disease Translational MedicineNanjing Medical University NanjingJiangsu 211166China

Background: The long-term predicted value of microvolt T-wave alternans (MTWA) for ventricular tachyarrhythmia in patients with arrhythmogenic right ventricular cardiomyopathy (ARVC) remains unclear. Our study explored the characteristics of MTWA and its prognostic value when combined with an electrophysiologic study (EPS) in patients with ARVC. Methods: All patients underwent non-invasive MTWA examination with modified moving average (MMA) analysis and an EPS. A positive event was defined as the first occurrence of sudden cardiac death, documented sustained ventricular tachycardia (VT), ventricular fibrillation, or the administration of appropriate implantable cardioverter defibrillator therapy including shock or antitachycardia pacing. Results: Thirty-five patients with ARVC (age 38.6 ± 11.0 years;28 males) with preserved left ventricular (LV) function were recruited. The maximal TWA value (MaxValt) was 17.0 (11.0–27.0)μV. Sustained VT was induced in 22 patients by the EPS. During a median follow-up of 99.9 ± 7.7 months, 15 patients had positive clinical events. When inducible VT was combined with the MaxValt, the area under the curve improved from 0.739 to 0.797. The receiver operating characteristic curve showed that a MaxValt of 23.5 μV was the optimal cutoff value to identify positive events. The multivariate Cox regression model for survival showed that MTWA (MaxValt, hazard ratio [HR], 1.06;95% confidence interval [CI], 1.01–1.11;P = 0.01) and inducible VT (HR, 5.98;95% CI, 1.33–26.8;P = 0.01) independently predicted positive events in patients with ARVC. Conclusions: MTWA assessment with MMA analysis complemented by an EPS might provide improved prognostic ability in patients with ARVC with preserved LV function during long-term follow-up.

关键词： Modified moving average analysis Microvolt T-wave alternans Electrophysiologic study Ventricular tachyarrhythmia Arrhythmogenic right ventricular cardiomyopathy

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

川滇高山栎QaGDU3基因的克隆及遗传转化

引用

植物科学学报 2022年第4期40卷 524-532页

作者：杨霖尹康权杜芳北京林业大学生态与自然保护学院北京100083 北京林业大学草业与草原学院北京100083

以川滇高山栎(Quercus aquifolioides Rehd. et Wils.)叶片为材料,分别采用不依赖于连接反应的克隆法、农杆菌花序侵染、实时荧光定量PCR、图像分析等方法克隆了QaGDU3基因,获得QaGDU3转基因拟南芥(Arabidopsis thaliana(L.)Heynh.)并... 详细信息

以川滇高山栎(Quercus aquifolioides Rehd. et Wils.)叶片为材料,分别采用不依赖于连接反应的克隆法、农杆菌花序侵染、实时荧光定量PCR、图像分析等方法克隆了QaGDU3基因,获得QaGDU3转基因拟南芥(Arabidopsis thaliana(L.)Heynh.)并分析转基因植物的表型变化,利用生物信息学对QaGDU3蛋白与其他物种的GDU3蛋白序列间的差异和进化关系进行了分析。结果显示,GDU3是种子植物特有的基因;QaGDU3属于阴离子渗透酶ArsB/NhaD超家族成员,可能具有氨基酸转运功能。QaGDU3基因在拟南芥中过表达能够激活水杨酸(SA)途径中AtPR1、AtACD6、AtCBP60g和AtPAD4基因的表达,且转基因拟南芥莲座大小随着QaGDU3基因表达量升高而变小。研究结果说明GDU3是种子植物所特有的氨基酸转运蛋白并参与植物生长发育的调控。

关键词：川滇高山栎序列分析基因功能

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Effects of nitrogen content on growth and hydraulic characteristics of peach(Prunus persica L.) seedlings under different soil moisture conditions

引用

Journal of Forestry Research 2014年第2期25卷 365-375页

作者： Zhi-liang Zhang Guo-dong Liu Fu-cang Zhang Cai-xia Zheng Fu-quan Ni yin-hong kang Yun Zeng College of Water Resource and Hydropower Sichuan University College of Information and Technology Sichuan Agricultural University Key Laboratory of Agricultural Soil and Water Engineering in Arid and Semiarid Areas of Ministry of Education Northwest Sci-Tech University of Agriculture and Forestry

A pot experiment was conducted to investigate the effects of nitrogen content [Nl （no fertilizer）, N2 （0.15 g.kg-l）, and N3 （0.3 g.kg 1）] on the growth and the hydraulic characteristics of peach seedlings under different soil moisture conditions （Wl, W2 and W3, in which the soil water content was 45% to 55%, 60% to 70%, and 75% to 80% of the field water capacity, respectively） by using a specialized high pressure flow meter with a root chamber and a coupling, which was connected to plant organs. Leaf area and leaf hydraulic conductivity （KL） increased significantly in the seedlings because of increased soil moisture and N content. KL increased with leaf area. A linear correlation was documented between KL and leaf area. KL was higher in the morning and began to decline sharply after 16：00, at which KL declined after an initial increase. Soil moisture and N content enhanced shoot （Ks） and root （Kr） hydraulic conductivities, thereby improving the low soil moisture condition to a large extent. Ks and Kr of the seedlings were reduced by 32% and 27% respectively in N~, and by 14.7% and 9.4%, respectively in N2, and both in Wb compared with the control treatment. N3 had no significant effect on Ks and Kr under similar conditions. Linear negative correlations were observed between Kr and the excised root diameter as well as between Ks and the shoot stem diameter. The shoot-to-root ratio increased with in- crease in N content. The shoot-to-root ratio in N3 was increased by 14.37%, compared with N1 in W1 as well as by 12% and 4.39% in Wz and W3, respectively. Knowledge of the effects of soil moisture and N fertilizer on hydraulic characteristics and growth is important. Our results provide basic guidelines for the implementation of water-saving irrigation and fertilization management of nursery stock.

关键词： nitrogen Hydraulic conductivity growth WUE Prunus persica L.

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

基于最小模糊误差熵的机动目标跟踪新方法

引用

电子学报 2023年第9期51卷 2408-2418页

作者：陈咏茵刘全仲李良群康莉深圳大学电子与信息工程学院广东深圳518060 深圳大学广东省智能信息处理重点实验室广东深圳518060 中国长城科技集团有限公司广东深圳518057

为了提高不同噪声影响下机动目标跟踪的性能,提出了一种基于最小模糊误差熵无迹滤波(Minimum Fuzzy Error Entropy Unscented Filter,MFEE-UF)机动目标跟踪新方法.在提出方法中,通过引入模糊隶属度表示不同误差样本对估计结果的不同影响... 详细信息

为了提高不同噪声影响下机动目标跟踪的性能,提出了一种基于最小模糊误差熵无迹滤波(Minimum Fuzzy Error Entropy Unscented Filter,MFEE-UF)机动目标跟踪新方法.在提出方法中,通过引入模糊隶属度表示不同误差样本对估计结果的不同影响,构建最小模糊误差熵准则(Minimum Fuzzy Error Entropy Criterion,MFEEC),解决了普通误差熵中的权重单一化问题,并利用该准则优化无迹滤波;在推导MFEE-UF过程中,首先利用无迹变换(Unscented Transformation,UT)框架得到先验状态估计和先验协方差估计,并通过系统重建得到误差信息,再根据MFEEC构建目标函数,最后利用定点迭代法递归求得后验状态估计结果和后验协方差估计.此外,本文采用一种自适应的核宽设置方法.实验结果表明,该算法能够具有良好的目标跟踪效果,且表现出较强的稳定性.

关键词：模糊误差熵无迹滤波自适应核宽状态估计目标跟踪

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

A High-Throughput Screening System for Arabidopsis Transcription Factors and Its Application to Med25-Dependent Transcriptional Regulation

引用

Molecular Plant 2011年第3期4卷 546-555页

作者： Bin Oua Kang-Quan Yin Sai-Nan Liu Yan Yang Tren Gu Jennifer Man Wing Hui Li Zhang Jin Miao Youichi Kondou Minami Matsui Hong-Ya Gu Li-Jia Qu National Laboratory for Protein Engineering and Plant Genetic Engineering College of Life Sciences Peking University Beijing 100871 People's Republic ofChina Plant Science Center RIKEN Yokohama Institute 1-7-22 Suehiro-cho Tsurumi-ku Yokohama City Kanagawa 230-0045 Japan The National Plant Gene Research Center (Beijing) Beijing 100101 People's Republic of China

The activities of transcription factors （TFs） require interactions with specific DNA sequences and other reg- ulatory proteins. To detect such interactions in Arabidopsis, we developed a high-throughput screening system with a Gateway-compatible Gal4-AD-TF library of 1589 Arabidopsis TFs, which can be easily screened by mating-based yeast-one-hybrid （YIH） and yeast-two-hybrid （Y2H） methods. The efficiency of the system was validated by examining two well-characterized TF-DNA and TF-protein interactions： the CHE-CCA1 promoter interaction by YIH and NPR1-TGAs interactions by Y2H. We used this system to identify eight TFs that interact with a Mediator subunit, Med25, a key reg- ulator in JA signaling. We identified five TFs that interacted with the GCC-box cis-element in the promoter of PDF1.2, a downstream gene of Med25. We found that three of these TFs, all from the AP2-EREBP family, interact directly both with Med25 and the GCC-box of PDF1.2, suggesting that Med25 regulates PDF1.2 expression through these three TFs. These results demonstrate that this high-throughput Y1H/Y2H screening system is an efficient tool for studying transcrip- tional regulation networks in Arabidopsis. This system will be available for other Arabidopsis researchers, and thus it provides a vital resource for the Arabidopsis community.

关键词： Transcription factors Arabidopsis yeast-one-hybrid yeast-two-hybrid Med25.

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

镉低积累青菜品种筛选及硫对镉胁迫下青菜镉含量和品质影响

引用

农业环境科学学报 2018年第8期37卷 1592-1601页

作者：杜小平康靖全吕金印西北农林科技大学生命科学学院陕西杨凌712100

以36个不同基因型青菜品种为材料,采用盆栽试验,根据可食部分生物量和镉(Cd)积累量差异,筛选Cd低积累和Cd高积累青菜品种,利用小区试验,研究外施硫对不同Cd积累型青菜品种可食部分Cd含量和营养品质的影响。结果表明:不同浓度Cd处理下,3... 详细信息

以36个不同基因型青菜品种为材料,采用盆栽试验,根据可食部分生物量和镉(Cd)积累量差异,筛选Cd低积累和Cd高积累青菜品种,利用小区试验,研究外施硫对不同Cd积累型青菜品种可食部分Cd含量和营养品质的影响。结果表明:不同浓度Cd处理下,36个青菜品种可食部分生物量和Cd含量差异显著;紫罗兰(ZLL)和矮脚黄(AJH)2个青菜品种符合Cd低积累品种特征,适宜在Cd中低污染土壤(Cd≤1 mg·kg^(-1))种植;壶瓶长梗白(HPCGB)和抗热605(KR605)2个青菜品种可食部分Cd含量较高,为Cd高积累品种。小区试验结果进一步验证了盆栽筛选出的Cd低积累和Cd高积累品种特性。1 mg·kg^(-1)Cd处理水平下,2个Cd低积累和2个Cd高积累青菜品种可食部分可溶性蛋白、可溶性糖和硝酸盐含量均高于对照;5 mg·kg^(-1)Cd处理水平下,上述4个青菜品种硝酸盐含量超过食品安全国家标准限量(GB 18406.1—2001);外施硫可不同程度降低青菜可食部分Cd含量和硝酸盐含量,提高了可食部分可溶性蛋白、可溶性糖和维生素C含量。在Cd中低污染大田种植紫罗兰(ZLL)和矮脚黄(AJH)青菜品种及外施硫可有效降低可食部分Cd含量和改善蔬菜品质。

关键词：青菜筛选 Cd低积累品种镉硫

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The regulators of BCR signaling during B cell activation

引用

Blood Science 2019年第2期1卷 119-129页

作者： Yue Wen Yukai Jing Lu Yang Danqing kang Panpan Jiang Na Li Jiali Cheng Jingwen Li Xingbo Li Zican Peng Xizi Sun Heather Miller Zhiwei Sui quan Gong Boxu Ren Wei yin Chaohong Liu Department of Pathogen Biology School of Basic MedicineHuazhong University of Science and TechnologyWuhanChina Department of Medical Imaging Technology Clinical and Molecular Immunology Research CenterMedical school of Yangtze UniversityJingzhouHubeiChina Department of Intracellular Pathogens National Institute of Allergy and Infectious DiseasesMT 59840USA Division of Medical and Biological Measurement National Institute of MetrologyBeijingChina Wuhan Children’s Hospital Tongji Medical CollegeHuazhong University of Science&TechnologyWuhanChina

B lymphocytes produce antibodies under the stimulation of specific antigens,thereby exerting an immune effect.B cells identify antigens by their surface B cell receptor(BCR),which upon stimulation,directs the cell to activate and differentiate into antibody generating plasma cells.Activation of B cells via their BCRs involves signaling pathways that are tightly controlled by various regulators.In this review,we will discuss three major BCR mediated signaling pathways(the PLC-g2 pathway,PI3K pathway and MAPK pathway)and related regulators,which were roughly divided into positive,negative and mutual-balanced regulators,and the specific regulators of the specific signaling pathway based on regulatory effects.

关键词： BCR MAPK PI3K PLC-g2 Regulator Signaling pathways

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Aberrant LETM1 elevation dysregulates mitochondrial functions and energy metabolism and promotes lung metastasis in osteosarcoma

引用

Genes & Diseases 2024年第3期11卷 368-384页

作者： Yulu Shi quan kang Hong Zhou Xiaohan Yue Yang Bi Qing Luo Stem Cell Biology and Therapy Laboratory The Children's Hospital of Chongqing Medical UniversityNational Clinical Research Center for Child Health and DisordersMinistry of Education Key Laboratory of Child Development and DisordersChongqing Key Laboratory of PediatricsChongqing 400014China Department of Pediatric Surgery The Children's Hospital of Chongqing Medical UniversityChongqing 400014China

Osteosarcoma is a differentiation-deficient disease,and despite the unique advan-tages and great potential of differentiation therapy,there are only a few known differentia-tion inducers,and little research has been done on their targets.Cell differentiation is associated with an increase in mitochondrial content and activity.The metabolism of some tu-mor cells is characterized by impaired oxidative phosphorylation,as well as up-regulation of aerobic glycolysis and pentose phosphate pathways.Leucine-containing zipper and EF-hand transmembrane protein 1(LETM1)is involved in the maintenance of mitochondrial morphology and is closely associated with tumorigenesis and progression,as well as cancer cell stemness.We found that MG63 and 143B osteosarcoma cells overexpress LETM1 and exhibit abnormalities in mitochondrial structure and function.Knockdown of LETM1 partially restored the mitochon-drial structure and function,inhibited the pentose phosphate pathway,promoted oxidative phosphorylation,and led to osteogenic differentiation.It also inhibited spheroid cell forma-tion,proliferation,migration,and invasion in an in vitro model.When LETM1 was knocked down in vivo,there was reduced tumor formation and lung metastasis.These data suggest that mitochondria are aberrant in LETM1-overexpressing osteosarcoma cells,and knockdown of LETM1 partially restores the mitochondrial structure and function,inhibits the pentose phosphate pathway,promotes oxidative phosphorylation,and increases osteogenic differentiation,thereby reducing malignant biological behavior of the cells.

关键词： Cancer metabolism Differentiation LETM1 Osteosarcoma Stemness

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：