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Journal of Groundwater Science and Engineering 2024年第3期12卷 339-346页

作者： Li-qiang Ge Yan-pei Cheng Qing Yi Xue-ru Wen Hua Dong Kun Liu jian-kang zhang National Research Center for Geoanalysis Beijing 100037China Institute of Hydrogeologv and Environimental Geology Chinese Academy of Geological SciencesShijiazhuang 050061China

The groundwater system is a unique ecosystem that serves both resource and ecological *** have conducted extensive theoretical research and practical work on groundwater ecological *** paper,based on the study of groundwater resources and surface ecology in the five Central Asian countries and adjacent areas of China,introduces the concept of ecosystem service *** establishes a groundwater ecological zoning index system and conducts research of ecological mapping using the five Central Asian countries and adjacent areas of China as *** this process,the ecosystem service functions of groundwater can be more comprehensively reflected,which can better guide regional geological environment protection and industrial *** approach helps coordinate the relationship between socio-economic development and water resource protection,maintain the health of the groundwater ecological environment,enhance the value of groundwater ecological services,and promote the sustainable development of regional economies and societies.

关键词： Groundwater ecosystem Groundwater ecological service Ecological zoning of groundwater Five Central Asian countries

Screening and cloning for proteins transactivated by the PS1TP5 protein of hepatitis B virus:A suppression subtractive hybridization study

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World Journal of Gastroenterology 2007年第10期13卷 1602-1607页

作者： jian-kang zhang Long-Feng Zhao Jun Cheng jiang Guo Dan-Qiong Wang Yuan Hong Yu Mao Department of Gastroenterology The First Hospital of Shanxi Medical University Taiyuan 030001 Shanxi Province China Institute of Infectious Diseases Beijing Ditan HospitalBeijing 100011 China

AIM： To clone and identify human genes transactivated by PSITP5 by constructing a cDNA subtractive library with suppression subtractive hybridization （SSH） technique. METHODS： SSH and bioinformatics techniques were used for screening and cloning of the target genes transactivated by PS1TP5 protein. The mRNA was isolated from HepG2 cells transfected with pcDNA3.1（-）- myc-his（A）-PS1TP5 and pcDNA3.1（-）-myc-his（A） empty vector, respectively, and SSH technique was employed to analyze the differentially expressed DNA sequence between the two groups. After digestion with restriction enzyme Rsa Ⅰ, small size cDNAs were obtained. Then tester cDNA was divided into two groups and ligated to the specific adaptor 1 and adaptor 2, respectively. The tester cDNA was hybridized with driver cDNA twice and subjected to nested PCR for two times, and then subcloned into T/A plasmid vectors to set up the subb-active library. Amplification of the library was carried out with E.. coil strain DH5α. The cDNA was sequenced and analyzed in GenBank with Vector NTI 9.1 and NCBI BLAST software after PCR amplification. RESULTS： The subtractive library of genes transactivated by PS1TP5 was constructed successfully. The amplified library contained 90 positive clones. Colony PCR showed that 70 clones contained 200-1000-bp inserts. Sequence analysis was performed in 30 clones randomly, and the full-length sequences were obtained by bioinformatics technique. Altogether 24 coding sequences were obtained, which consisted of 23 known and 1 *** novel gene with unknown functions was found and named as PSITP5TP1 after being electronically spliced, and deposited in GenBank （accession number： DQ487761）. CONCLUSION： PSITP5 is closely correlated with immunoregulation, carbohydrate metabolism, signal transduction, formation mechanism of hepatic fibrosis, and occurrence and development of tumor. Understanding PSlTP5 transactive proteins may help to bring some new clues for further studying the biological f

关键词： Hepatitis B virus Pre-S1 protein Suppression subtractive hybridization

Effect of annealing treatment on microstructures and properties of austenite-based Fe-28Mn-9Al-0.8C lightweight steel with addition of Cu

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China Foundry 2021年第3期18卷 207-216页

作者： Zhuo Chen Ming-xiang Liu jian-kang zhang Lei Yang Yun-hu zhang Chang-jiang Song Qi-jie Zhai Center for Advanced Solidification Technology(CAST) School of Materials Science and EngineeringShanghai UniversityShanghai 200444China

The mechanical properties of an austenite-based Fe-Mn-Al-C lightweight steel were improved by co-precipitation of nanoscale Cu-rich and κ-carbide *** Fe-28Mn-9Al-0.8C-(0,3)Cu (wt.%) strips were near-rapidly solidified and annealed in the temperature range from 500 ℃ to 700 ℃.The microstructure evolution and mechanical properties of the steel under different annealing processes were *** analysis reveals that nanoscale κ-carbides and Cu-rich particles precipitate in the austenite and ferrite of the steel in this annealing temperature ***-precipitation of nanoscale Cu-rich particles and κ-carbides provides an obvious increment in the yield *** the annealing temperature of 600 ℃,both the yield strength and ultimate tensile strength of Fe-28Mn-9Al-0.8C-3Cu (wt.%) steel strip are the *** total elongation is 25%,which is obviously higher than that of Cu-free steel strips,for the addition of Cu reduces the large sized κ-carbides precipitated along austenite/ferrite *** the annealing temperature rises to 700 ℃,the strength and ductility of the two steel strips deteriorate due to the formation of massive intergranular κ-carbides precipitated along austenite/ferrite *** can be concluded that a proper co-precipitation of Cu-rich particles and κ-carbides would improve the properties of austenite-based Fe-Mn-Al-C steel.

关键词： austenite-based steel Cu-rich particle near-rapid solidification co-precipitation strengthening annealing treatment

Synthesis and preliminary biological evaluation of a technetium-99m labeled thymidine analog

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Chinese Chemical Letters 2011年第11期22卷 1309-1312页

作者： Chun Xiong Lu Zheng Wu Wang Quan Fu jiang Jie Tang Cheng Tan jian kang-zhang Key Laboratory of Nuclear Medicine Ministry of Health/Jiangsu Key Laboratory of Molecular Nuclear Medicine Jiangsu Institute of Nuclear Medicine Wuxi 214063 China School of Chemical ＆ Material Engineering Jiangnan University Wuxi 214122 China Department of Food Science ＆ Technology School of Agriculture and Biology Shanghai Jiao Tong University Shanghai 200240 China

The synthesis and labeling of ^（99m）Tc-N^3-（N＇-[2-sulfanyl-ethylamino）acetyl]-2-aminoethyl-sulfanyl-1-hexanamide}thymidine （^（99m）Tc-NHT） were *** the presence of sodium glucoheptonate（GH） and ethylene diamine tetraacetic acid（EDTA）,^（99m）Tc-NHT was obtained by using bisaminoethanethiol（N_2S_2） as a bifunctional coupling *** radiochemical purity of the ^（99m）Tc-NHT was over 95%.Biodistribution of ^（99m）Tc-NHT was performed in hepatoma HepA tumor-bearing *** 2 h p.i.,the ratios of tumor-to-muscle,tumor-to-bone and tumor-to-blood were 4.41±0.32,2.45±0.24 and 1.51±0.18,respectively.

关键词： Synthesis ^(99m)Tc-NHT Biodistribution Thymidine analog Tumor imaging

Ecological function zoning and protection of groundwater in Asia

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Journal of Groundwater Science and Engineering 2021年第4期9卷 359-368页

作者： Xue-ru Wen Yan-pei Cheng jian-kang zhang Hua Dong Institute of Hydrogeology and Environmental Geology CAGSShijiazhuang 050061China Key Laboratory of Groundwater Sciences and Engineering Ministry of Natural ResourcesShijiazhuang 050061China.

The natural groundwater recharge in Asia is estimated to be 4677.74×10^(9)m^(3)/***,it features extremely uneven spatial-temporal *** is distributed in various natural and geological environments,and it is liable to be affected by numerous factors and possesses different ***,groundwater faces complex ecological *** paper gains a complete understanding of groundwater in Asia in terms of the structure of aquifer systems,the processes of groundwater cycle,and the spatial variation laws of surface *** on this,it proposes the ecological function zoning scheme of groundwater in Asia,aiming to provide guidance for the utilization of regional water resources and the planning for economic and social development,coordinate the relationship between social and economic development and water resource protection,and improve the ecological functions of ***,this paper analyzes the problems with regional groundwater management in Asia and puts forward countermeasures and suggestions,thus providing a theoretical basis for the sustainable development and utilization of regional groundwater and environmental protection.

关键词： Asia Groundwater resources Eco-environment index system Function zoning

Steel-refractory reactions in lanthanum-,cerium-,and ytrium-added steels

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Journal of Iron and Steel Research International 2024年第6期31卷 1473-1485页

作者： jian kang Hong-po Wang Yu Wang College of Materials Science and Engineering Chongqing UniversityChongqing 400044China The State Key Laboratory of Mechanical Transmissions Chongqing UniversityChongqing 400044China

A systematic experimental study was conducted on the similarities and differences in the reactions between commonly used rare earth elements(REEs)and frequently used refractories in the steelmaking *** results indicate that the reaction behaviors of La,Ce,and Y with MgO,Al_(2)O_(3),and MgO·Al_(2)O_(3) crucibles differed *** consumption rates of La,Ce,and Y in the molten steel were in descending order of La>Ce>Y for smelting with all three kinds of *** the REEs reacted with the crucible,the contents of Mg and Al showed a trend of first increasing and then *** laboratory-scale smelting conditions,the reactions related to REEs were dominated by steel-crucible reactions,and the contact area between the unit mass of molten steel and refractory materials mainly determined the consumption rates of ***-and Ce-crucible reactions generated loose and porous reaction interfaces;however,Y-crucible reactions generated a dense Y_(2)O_(3) layer,dramatically suppressing the consumption rate of *** calculation indicated that the reactions between rare earth and different refractory materials were first-order *** crucible materials significantly affected the product type and morphology of the steel-crucible reaction interfaces,thereby influencing the consumption rate of *** reactions between REEs and refractories must be seriously considered,especially for small-scale smelting.

关键词： Rare earth Inclusion Refractory Thermodynamics Dynamics

SNORD126 promotes HCC and CRC cell growth by activating the PI3K-AKT pathway through FGFR2

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Journal of Molecular Cell Biology 2017年第3期9卷 243-255页

作者： Xianlong Fang Dongmei Yang Hongping Luo Shuai Wu Wenjie Dong Jing Xiao Sujing Yuan Aimin Ni kang-jian zhang Xin-Yuan Liu Liang Chu State Key Laboratory of Cell Biology Institute of Biochemistry and Cell Biology Shanghai Institutes for Biological Sciences Chinese Academy of Sciences Shanghai 200031 China Xinyuan Institute of Medicine and Biotechnology Zhejiang Sci-Tech University Hangzhou Zhejiang 310018 China Hepatic Surgery Center Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China

Small nucleolar RNA （snoRNA） dysfunctions have been associated with cancer development. SNORD126 is an orphan C/D box snoRNA that is encoded within introns 5-6 of its host gene, cyclin Bl-interacting protein 1 （CCNBIIP1）. The cancer-associated molecular mechanisms triggered by SNORD126 are not fully understood. Here, we demonstrate that SNORD126 is highly expressed in hepatoceUular carcinoma （HCC） and colorectal cancer （CRC） patient samples. SNORD126 increased Huh-7 and SW480 cell growth and tumorigenicity in nude mice. Knockdown of SNORD126 inhibited HepG2 and LS174T cell growth. We veri- fied that SNORD126 was not processed into small RNAs with miRNA activity. Moreover, SNORD126 did not show a significant expression correlation with CCNBIlP1 in HCC samples or regulate CCNBIlP1 expression. Our gene expression profile analysis indicated that SNORD126-upregulated genes frequently mapped to the PI3K-AKT pathway. SNORD126 overexpression increased the levels of phosphorylated AKT, GSK-3p, and p7056K and elevated fibroblast growth factor receptor 2 （FGFR2） expression. siRNA-mediated knockdown or AZD4547-mediated inactivation of FGFR2 in SNORD126-overexpressing Huh-7 cells inhibited AKT phosphorylation and suppressed cell growth. These findings indicate an oncogenic role for SNORD126 in cancer and suggest its potential as a therapeutic target.

关键词： small nucleolar RNA HCC CRC FGFR2 PI3K-AKT

Recent progress in carbene-catalyzed fluoroalkylation

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Science China Chemistry 2024年第8期67卷 2448-2460页

作者： Bei zhang Xuan zhang jian Wang jiangsu Key Laboratory of Atmospheric Environment Monitoring and Pollution Control Collaborative Innovation Center of Atmospheric Environment and Equipment TechnologySchool of Environmental Science and EngineeringNanjing University of Information Science and TechnologyNanjing 210044China School of Pharmaceutical Sciences Tsinghua UniversityBeijing 100084China

The fluoroalkyl fragment plays a vital role in various fields such as pharmaceuticals and materials science, and has a significant impact on human life not only on the present but also in the future. The insertion of fluoroalkyl groups into the chemical skeletons can cause considerable alternations in the chemical, physical and biological properties of molecules, which has attracted numerous scientists from different fields. This review focuses on N-heterocyclic carbene(NHC)-catalyzed fluoroalkylation reactions, providing a detailed analysis and summary of the achievements in the past two decades. Many successful examples clearly indicate that NHCs can catalyze the tranformation of fluoroalkylation reagents with various electronic characteristics, whether electrophilic, nucleophilic, or radical precursors. Herein, the fluoroalkylation reactions are classified based on different catalytic modes of NHCs: Lewis base catalysis, Umploung catalysis, radical catalysis and others. Meanwhile, by refining and systematically studying the contribution of NHC catalysis in fluoroalkylation reactions, this review is expected to provide valuable insights and enlightenment for related fields.

关键词： N-heterocyclic carbene fluoroalkylation radical catalysis

Nanophononic metamaterials induced proximity effect in heat flux regulation

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Frontiers of physics 2024年第2期19卷 149-156页

作者： jian zhang Haochun zhang Gang zhang School of Energy Science and Engineering Harbin Institute of TechnologyHarbin 150001China Institute of High Performance Computing Agency for ScienceTechnology and Research(A*STAR)Singapore 138632Singapore

Recent studies have shown that the construction of nanophononic metamaterials can reduce thermal conductivity without affecting electrical properties,making them promising in many fields of application,such as energy conversion and thermal ***,although extensive studies have been carried out on thermal conductivity reduction in nanophononic metamaterials,the local heat flux characteristic is still *** this work,we construct a heat flux regulator which includes a silicon nanofilm with silicon *** regulator has remarkable heat flux regulation ability,and various impacts on the regulation ability are ***,even in the region without nanopillars,the local heat current is still lower than that in pristine silicon nanofilms,reduced by the neighboring nanopillars through the thermal proximity *** combine the analysis of the phonon participation ratio with the intensity of localized phonon modes to provide a clear *** findings not only provide insights into the mechanisms of heat flux regulation by nanophononic metamaterials,but also will open up new research directions to control local heat flux for a broad range of applications,including heat management,thermoelectric energy conversion,thermal cloak,and thermal concentrator.

关键词： nanophononic metamaterials proximity effect

Regulatory RNAs in Bacillus subtilis:A review on regulatory mechanism and applications in synthetic biology

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Synthetic and Systems Biotechnology 2024年第2期9卷 223-233页

作者： Anqi Peng Guobin Yin Wenjie Zuo Luyao zhang Guocheng Du jian Chen Yang Wang Zhen kang The Science Center for Future Foods Jiangnan UniversityWuxi214122China The Key Laboratory of Carbohydrate Chemistry and Biotechnology Ministry of EducationSchool of BiotechnologyJiangnan UniversityWuxi214122China The Key Laboratory of Industrial Biotechnology Ministry of EducationSchool of BiotechnologyJiangnan UniversityWuxi214122China

Bacteria exhibit a rich repertoire of RNA molecules that intricately regulate gene expression at multiple hierarchical levels,including small RNAs(sRNAs),riboswitches,and antisense ***,the majority of these regulatory RNAs lack or have limited protein-coding capacity but play pivotal roles in orchestrating gene expression by modulating transcription,post-transcription or translation *** and redesigning these regulatory RNA elements have emerged as pivotal strategies in the domains of metabolic engineering and synthetic *** previous investigations predominantly focused on delineating the roles of regulatory RNA in Gram-negative bacterial models such as Escherichia coli and Salmonella enterica,this review aims to summarize the mechanisms and functionalities of endogenous regulatory RNAs inherent to typical Gram-positive bacteria,notably Bacillus ***,we explore the engineering and practical applications of these regulatory RNA elements in the arena of synthetic biology,employing *** as a foundational chassis.

关键词： Regulatory RNA devices Small RNAs Riboswitches Synthetic biology Bacillus subtilis