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Journal of Integrative Agriculture 2015年第2期14卷 305-313页

作者： SUN He LANG Zhi-hong LU Wei zhang Jie HE kang-lai ZHU Li LIN Min HUANG da-fang Biotechnology Research Institute Chinese Academy of Agricultural Sciences State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant Protection Chinese Academy of Agricultural Sciences

Using linker peptide LP4/2A for multiple gene transformation is considered to be an effective method to stack or pyramid several traits in plants. Bacillus thuringiensis（Bt） cry gene and epsps（5-enolpyruvylshikimate-3-phosphate synthase） gene are two important genes for culturing pest-resistant and glyphosate-tolerant crops. We used linker peptide LP4/2A to connect the Bt cry1 Ah gene with the 2m G2-epsps gene and combined the wide-used man A gene as a selective marker to construct one coordinated expression vector called p2 EPUHLAGN. The expression vector was transferred into maize by Agrobacterium tumefaciens-mediated transformation, and 60 plants were obtained, 40% of which were positive transformants. Molecular detection demonstrated that the two genes in the fusion vector were expressed simultaneously and spliced correctly in translation processing; meanwhile bioassay detection proved the transgenic maize had preferable pest resistance and glyphosate tolerance. Therefore, linker peptide LP4/2A provided a simple and reliable strategy for producing gene stacking in maize and the result showed that the fusion gene transformation system of LP4/2A was feasible in monocot plants.

关键词： LP4/2A gene stacking transgenic maize insect resistance glyphosate tolerance

Unequal error protection scheme for layered sources transmission over MIMO systems using spatial diversity and multiplexing technology

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The Journal of China Universities of Posts and Telecommunications 2015年第3期22卷 56-63页

作者： zhang Yongjian zhang Kexin kang Yanmei Yang dacheng School of Telecommunication Engineering Beijing University of Posts and Telecommunications University of International Relations Academic office Beijing University of Posts and Telecommunications

This paper proposes a hybrid scheme of the Alamouti coding and vertical bell labs layered space-time （V-BLAST） with the zero-forcing （ZF） receiver, which can achieve the unequal error protection （UEP） transmission in terms of the differences of importance in the layered sources. We analyze the tradeoff between spatial diversity and multiplexing in a mathematical manner, and prove the monotonic behavior of the crosspoint. Based on this scheme, we adopt V-BLAST to provide high data rates for the enhancement layer （EL） bitstreams, and adopt Alamouti coding to guarantee high reliability for the base layer （BL） bitstreams. Meanwhile, to improve the transmission reliability for the layered sources under the multiple-input multiple-output （MIMO） system with the limited-bandwidth and the total limited power, and to raise the frequency bandwidth efficiency, we take the power allocation considered in this proposed scheme. The simulation results show that the hybrid scheme has the average 1.9 dB and 1.3 dB gain in video peak signal-to-noise ratio （PSNR）, compared with the pure spatial diversity and pure spatial multiplexing scheme, respectively.

关键词： unequal error protection spatial diversity and multiplexing outage probability multiple-input multiple-output (MIMO) powerallocation

A highly selective catalyst of Co/La_(4)Ga_(2)O_(9) for CO_(2) hydrogenation to ethanol

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Journal of Energy Chemistry 2021年第5期30卷 486-495页

作者： kang An Siran zhang Jiaming Wang Qiang Liu Ziyang zhang Yuan Liu Department of Catalysis Science and Technology School of Chemical EngineeringTianjin UniversityTianjin 300350China Collaborative Innovation Center of Chemical Science and Engineering(Tianjin) Tianjin 300072China

A new catalyst of Co/La_(4)Ga_(2)O_(9) for CO_(2) hydrogenation to produce ethanol was prepared by reducing LaCo^(0).5 Ga0.5 O3,which showed excellent selectivity to ethanol(%35 C-mol%)at mild reaction conditions(270°C,3.5 MPa,3000 m L g-1 h-1).The catalysts were characterized by N_(2) adsorption/desorption,XRD,XAFS,CO and CO_(2)-TPD,H2 chemisorption,XPS and TEM *** interaction between Co nanoparticles(NPs)and La2+4 Ga_(2)O_(9) oxide resulted in Co^(0)-Coon the surface of Co *** was proposed that La_(4)Ga_(2)O_(9) could catalyze reverse water gas shift reaction(r-WGS),which converted CO_(2) to ***,the CO migrated to Co^(0)-Co^(2+)on Co NPs,where it was hydrogenated to form ethanol like higher alcohols synthesis from *** results suggest that by controlling the oxidation state of cobalt,and combined with a kind of active site for activating CO_(2) to form CO,a catalyst with excellent selectivity to ethanol could be obtained for CO_(2) hydrogenation,which means that the complex reaction may be proceed with high selectivity using only one active metal component.

关键词： CO_(2)hydrogenation Ethanol synthesis Perovskite Gallium Co nanoparticles

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参姜止痢合剂的抗炎镇痛功效评价

中国兽医杂志 2025年第1期61卷 128-132页

作者：刘莲郭志廷张康张景艳王磊李建喜中国农业科学院兰州畜牧与兽药研究所农业农村部兽用药物创制重点实验室甘肃省中兽药工程技术研究中心甘肃兰州730050

为探究中兽药参姜止痢合剂的抗炎、镇痛作用,本试验采用二甲苯致小鼠耳廓肿胀抑制试验建立炎症模型,采用醋酸扭体试验和热板法镇痛试验建立疼痛模型,各试验造模前将昆明小鼠随机分为5个组,分别为阴性对照组(灌胃生理盐水)、阳性药物对照... 详细信息

为探究中兽药参姜止痢合剂的抗炎、镇痛作用,本试验采用二甲苯致小鼠耳廓肿胀抑制试验建立炎症模型,采用醋酸扭体试验和热板法镇痛试验建立疼痛模型,各试验造模前将昆明小鼠随机分为5个组,分别为阴性对照组(灌胃生理盐水)、阳性药物对照组[醋酸地塞米松片(抗炎),阿司匹林肠溶片(镇痛)]、参姜止痢合剂高剂量组(1 g/mL,0.4 mL)、参姜止痢合剂中剂量组(1 g/mL,0.2 mL)和参姜止痢合剂低剂量组(1 g/mL,0.1 mL),每组12只。连续预处理6 d后造模,观察并计算小鼠耳肿胀度和耳肿胀抑制率,记录小鼠扭体潜伏期,计算疼痛抑制率,测定不同检测时间点的痛阈值,以评价参姜止痢合剂的抗炎、镇痛作用。结果显示,与阴性对照组相比,中、高剂量参姜止痢合剂和阳性药物预处理6 d可极显著抑制二甲苯致小鼠耳肿胀度(P<0.01),且中剂量组耳肿胀抑制率最高,达到72%;参姜止痢合剂中、高剂量组和阳性药物对照组小鼠腹腔注射醋酸后扭体潜伏期均延长,且15 min内出现的扭体次数显著降低(P<0.05或P<0.01);中、高剂量参姜止痢合剂和阳性药物在末次预处理后1.5 h均能显著增加小鼠的痛阈值(P<0.05或P<0.01)。结果表明,参姜止痢合剂具有良好的抗炎、镇痛作用,为参姜止痢合剂的临床应用提供了参考依据。

关键词：中兽药参姜止痢合剂抗炎镇痛

Sink or source? Methane and carbon dioxide emissions from cryoconite holes, subglacial sediments, and proglacial river runoff during intensive glacier melting on the Tibetan Plateau

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Fundamental Research 2021年第3期1卷 232-239页

作者： Yulan zhang Shichang kang da Wei Xi Luo Zhuangzhuang Wang Tanguang Gao State Key Laboratory of Cryosphere Sciences Northwest Institute of Eco-Environment and ResourcesChinese Academy of SciencesLanzhou 730000China CAS Center for Excellence in Tibetan Plateau Earth Sciences Chinese Academy of SciencesBeijing 100101China Key Laboratory of Western China's Environmental Systems(Ministry of Education) College of Earth and Environmental SciencesLanzhou UniversityLanzhou 730000China Key Laboratory of Mountain Surface Processes and Ecological Regulation Institute of Mountain Hazards and EnvironmentChinese Academy of SciencesChengdu 610041China University of Chinese Academy of Sciences Beijing 100049China

High Mountain Asia glaciers are currently ignored in the estimation of global greenhouse gas budgets (e.g., methane (CH4) and carbon dioxide (CO_(2))). Similar to the Asian Water Tower and Third Pole, the Tibetan Plateau (TP) hosts the largest volume of glaciers outside the polar regions. These glaciers contain large reservoirs of organic carbon that can influence glacial ecosystems under rapid melting. However, no data exist on the current footprint of CH4 and CO_(2) from glaciers in the TP. Here, we report in situ observations of CH4 and CO_(2) fluxes for glacial cryoconite holes, subglacial sediments, and proglacial river runoff across the TP. Our results indicate that cryoconite holes and subglacial sediments can accelerate the export of greenhouse gasses during the melting season due to intensive glacier melting. However, to some extent, proglacial river runoff can be a significant sink of atmospheric CO_(2);this fact was not identified in previous studies. Our findings suggest that variations (source or sink) of greenhouse gasses from TP glacial basins should be considered in regional CH4 and CO_(2) budgets under climate warming.

关键词： Glacier Methane Carbon dioxide Climate change Tibetan Plateau

Hydrolysis of epoxides and aziridines catalyzed by polymer-supported quarternary ammonium bisulfate

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Chinese Chemical Letters 2008年第2期19卷 146-148页

作者： Wan Xuan zhang kang Ye Ministry-of-Education Key Laboratory for the Synthesis and Application of Organic Functional Molecules Hubei University Wuhan 430062 China

Macroporous resin （D201）-supported quartemary ammonium bisulfate （D201-HSO4） was prepared and effectively used in catalyzing the hydrolysis of epoxides or aziridines under mild and non-metal conditions to give the... 详细信息

关键词： Resin-supported quartemary ammonium bisulfate Catalysis Hydrolysis Aziridine Epoxide

Effects of irrigation efficiency on chemical transport processes

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Science China(Technological Sciences) 2009年第11期52卷 3287-3292页

作者： WANG kang zhang RenDuo SHENG Feng State Key Laboratory of Water Resources and Hydropower Engineering Science Wuhan UniversityWuhan 430072China School of Environmental Science and Engineering Sun Yat-sen UniversityGuangzhou 510275China

Irrigation practices greatly affect sustainable agriculture development. In this study, we investigated the effects of irrigation efficiency on water flow and chemical transport in soils, which had significant impact on the environment. Field dye staining experiments were conducted at different soils with various irrigation amount. Image analysis was conducted to study the heterogeneous flow patterns and their relationships with the irrigation efficiency. Irrigation efficiency and its environmental effects were evaluated using various indictors, including application efficiency, deep percolation ratio, storage efficiency, and uniformity. Under the same irrigation condition, soil chemical distributions were more heterogeneous than soil water distributions. The distributions were mainly affected by soil texture, initial soil water content, and irrigation amount. Storage efficiency, irrigation uniformity, and deep percolation ratio increased with irrigation amount. Since the chemical distribution uniformity was lower than the water uniformity, the amount of chemical leaching increased sharply with decrease of irrigation uniformity, which resulted in high environmental risks of groundwater pollution.

关键词： irrigation efficiency chemical transport groundwater pollution

Dendritic Cell as Therapeutic Vaccines against Tumors and Its Role in Therapy for Hepatocellular Carcinoma

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Cellular & Molecular Immunology 2006年第3期3卷 197-203页

作者： kang Sun Liang Wang Yanyun zhang Department of General Surgery the First Affiliated Hospital of Soochow University Suzhou 215006 China Shanghai Institute of Immunology Medical School of Shanghai Jiaotong University Shanghai 200025 China

Dendritic cells （DCs） are the most potent professional antigen-presenting cells, and capable of stimulating naive T cells and driving primary immune responses. DCs are poised to capture antigen, migrate to draining lymphoid organs, and after a process of maturation, select antigen-specific lymphocytes to which they present the processed antigen, thereby inducing immune responses. The development of protocols for the ex vivo generation of DCs may provide a rationale for designing and developing DC-based vaccination for the treatment of tumors. There are now several strategies being applied to upload antigens to DCs and manipulate DC vaccines. DC vaccines are able to induce therapeutic and protective antitumor immunity. Numerous studies indicated that hepatocellular carcinoma （HCC） immunotherapies utilizing DC-presenting tumor-associated antigens could stimulate an antitumour T cell response leading to clinical benefit without any significant toxicity. DC-based tumor vaccines have become a novel immunoadjuvant therapy for HCC. Cellular ＆ Molecular Immunology. 2006;3（3）：197-203.

关键词： dendritic cell vaccination hepatocellular carcinoma

Characteristics of Spatial and Temporal Variations of Monthly Mean Surface Air Temperature over Qinghai-Tibet Plateau

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Chinese Geographical Science 2006年第4期16卷 351-358页

作者： zhang Qianggong kang Shichang YAN Yuping Institute of Tibetan Plateau Research Chinese Academy of Sciences Beijing 100085 China Graduate University of Chinese Academy of Sciences Beijing 100049 China Key Laboratory of Cryosphere and Environment Chinese Academy of Sciences Lanzhou 730000 China National Climate Center Bering 100081 China

The recorded meteorological data of monthly mean surface air temperature from 72 meteorological stations over the Qinghal-Tibet Plateau in the period of 1960-2003 have been analyzed by using Empirical Orthogonal Function （EOF） method, to understand the detailed features of its temporal and spatial variations. The results show that there was a high consistency of the monthly mean surface air temperature, with a secondarily different variation between the north and the south of the plateau. Warming trend has existed at all stations since the 1960s, while the warming rates were different in various zones. The source regions of big rivers had intense warming tendency. June, November and December were the top three fast-warming months since the 1960s; while April, July and September presented dramatic warming tendency during the last decade.

关键词： monthly mean surface air temperature climatic variation EOF analysis Qinghai-Tibet Plateau

Identification of QTLs for yield and yield components of barley under different growth conditions

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2010年第3期11卷 169-176页

作者： da-wei XUE Mei-xue ZHOU Xiao-qin zhang Song CHEN kang WEI Fan-rong ZENG Ying MAO Fei-bo WU Guo-ping zhang College of Life and Environment Sciences Hangzhou Normal University Hangzhou 310036 China Department of Agronomy Zhejiang University Hangzhou 310029 China Tasmanian Institute of Agricultural Research University of Tasmania Kings Meadows TAS 7249 Australia

Waterlogging is a major abiotic stress limiting barley (Hordeum vulgare L.) yield and its stability in areas with excessive *** of genomic regions influencing the response of yield and its components to waterlogging stress will enhance our understanding of the genetics of waterlogging tolerance and the development of more tolerant barley *** trait loci (QTLs) for grain yield and its components were identified using 156 doubled haploid (DH) lines derived from a cross between the cultivars Yerong (waterlogging-tolerant) and Franklin (waterlogging-sensitive) grown under different conditions (waterlogged and well drained).A total of 31 QTLs were identified for the measured characters from two experiments with two growth *** phenotypic variation explained by individual QTLs ranged from 4.74% to 55.34%.Several major QTLs determining kernel weight (KW),grains per spike (GS),spikes per plant (SP),spike length (SL) and grain yield (GY) were detected on the same region of chromosome 2H,indicating close linkage or pleiotropy of the gene(s) controlling these *** different QTLs were identified under waterlogging conditions,and thus different markers may have to be used in selecting cultivars suitable for high rainfall areas.

关键词： Barley (Hordeum vulgare L.) Waterlogging tolerance Yield Quantitative trait locus (QTL)