检索结果-南通市图书馆

Study on PRRSV Receptor Genes Differential Expression in Lung Tissues in Different Breeds of Pigs after Infecting with HP-PRRSV

引用

Animal Husbandry and Feed Science 2017年第4期9卷 229-233,258页

作者： kang runmin Ji Gaosheng Zeng Kai Lv Xuebin Yin Mingyu Animal Breeding and Genetics Key Laboratory of Sichuan Province Sichuan Animal Science Academy Animal Disease Prevention and Food Safety Key Laboratory of Sichuan Province Key Laboratory of Bio-resources and Ecoenvironment of Ministry of EducationCollege of Life SciencesSichuan University

[ Objective] In order to study the susceptibility molecular mechanism of highly pathogenic porcine reproductive and respiratory syndrome virus （ HP- PRRSV） JXA1 isolate on Tibetan pig, Zangmei pig and Yorkshire pig. [ Method ] In the study, real-time quantitative RT-PCR method was established to compare and analyze the differential expression of five porcine reproductive and respiratory syndrome virus （PRRSV） receptor genes （HSPG2, SIGLEC1, CD163, VIM and NMMHC-H A） in lung tissues in Tibetan pig, Zangmei pig and Yorkshire pig before the challenge and at the 4th ,7th and 14th days after the challenge with JXAI isolate. [ Results ] HSPG2 expression in Tibetan pig lung tissues increased significantly at the 4th and 14th days after the challenge with JXAI （ P 〈 0.05 ）, while decreased significantly at the 7th day after the challenge （P 〈 0.05 ）, HSPG2 expression in Zangmei pig lung tissues increased significantly at the 14th day after the challenge （P〈0.05）. SIGLECl expression in Tibetan pig lung tissues increased significantly at the 4th and 14th days after the infection（P 〈 0.05 ）, while SIGLEC 1 expression in Yorkshire pig decreased significantly at the 4th, 7th and 14th days after the challenge （P 〈0. 05 ）. CD163 expression in lung tissues of Tibetan pig and Zangmei pig both increased significantly at the 14th day after the challenge （P 〈 0.05 ）, while CD163 expression in lung tissues of Yorkshire pig decreased significantly at the 7th and 14th days after the challenge （ P 〈 0. 05 ）. VIM expression in lung tissues of Tibetan pig increased significantly at the 7th day after the challenge （ P 〈 0. 05 ）, while which of Yorkshire pig at the 7th day after the challenge decreased significantly （ P 〈 0. 05 ）. NMMHC-II A expression in lung tissues of Zangmei pig increased significantly at the 4th day after the challenge （ P 〈 0. 05 ）, and which of Yorkshire pig increased significantly at the 4th and 14th days after the challenge （P 〈 0. 05 ）. [ Conclusion] SIGLEC1 and VIM

关键词： JXA1 isolate Tibetan pig Zangmei pig Yorkshire pig Porcine reproductive and respiratory syndrome virus receptor genes Differential expression

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

PRRSV Nsp1α蛋白结构及生物学功能研究进展

引用

黑龙江畜牧兽医 2024年第23期 39-44页

作者：李兴玉李金海吴学婧肖璐康润敏四川省畜牧科学研究院成都610066 华派生物集团成都641402

猪繁殖与呼吸综合征病毒(Porcine reproductive and respiratory syndrome virus, PRRSV)为有囊膜不分节段单股正链RNA病毒,基因组全长约15 kb,病毒基因组编码11个开放阅读框(open reading frame, ORFs),其中ORF1a编码多聚蛋白pp1a, pp1a通过自身的蛋白酶功能产生Nsp1α、Nsp1β、Nsp2~8蛋白。Nsp1α蛋白被认为是干扰素的拮抗剂,在抑制宿主干扰素产生过程中发挥着重要的作用,还在免疫调节、细胞凋亡等方面发挥作用。文章综述了Nsp1α蛋白结构及其生物学功能的研究进展,以期为PRRSV致病机制的研究及防治猪繁殖与呼吸综合征提供理论支撑。

关键词：猪繁殖和呼吸综合征病毒 Nsp1α蛋白结构生物学功能干扰素

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

The autophagy-lysosome pathway:a potential target in the chemical and gene therapeutic strategies for Parkinson’s disease

引用

Neural Regeneration Research 2025年第1期20卷 139-158页

作者： Fengjuan Jiao Lingyan Meng kang Du Xuezhi Li School of Mental Health Jining Medical UniversityJiningShandong ProvinceChina Shandong Collaborative Innovation Center for Diagnosis Treatment and Behavioral Interventions of Mental DisordersInstitute of Mental HealthJining Medical UniversityJiningShandong ProvinceChina

Parkinson’s disease is a common neurodegenerative disease with movement disorders associated with the intracytoplasmic deposition of aggregate proteins such asα-synuclein in *** one of the major intracellular degradation pathways,the autophagy-lysosome pathway plays an important role in eliminating these *** evidence has shown that upregulation of the autophagy-lysosome pathway may contribute to the clearance ofα-synuclein aggregates and protect against degeneration of dopaminergic neurons in Parkinson’s ***,multiple genes associated with the pathogenesis of Parkinson’s disease are intimately linked to alterations in the autophagy-lysosome ***,this pathway appears to be a promising therapeutic target for treatment of Parkinson’s *** this review,we briefly introduce the machinery of ***,we provide a description of the effects of Parkinson’s disease–related genes on the autophagy-lysosome ***,we highlight the potential chemical and genetic therapeutic strategies targeting the autophagy–lysosome pathway and their applications in Parkinson’s disease.

关键词： autophagy chemical therapy gene therapy Parkinson’s disease α-synuclein

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

诃子等中药组合对猪呼吸系统疾病病原菌体外抑制作用研究

引用

中兽医医药杂志 2023年第3期42卷 45-49页

作者：曹冶谢晶康润敏于吉锋四川省畜牧科学研究院四川成都610066 动物遗传育种四川省重点实验室四川成都610066

猪呼吸系统疾病一般由病毒和细菌混合感染引起,严重影响生猪健康,目前主要采用大剂量抗生素来治疗病猪,但易产生副作用。筛选适宜中药来治疗猪呼吸系统疾病是养猪业发展的迫切需求。本研究将中药诃子、余甘子用中性去离子水索氏提取4~6... 详细信息

猪呼吸系统疾病一般由病毒和细菌混合感染引起,严重影响生猪健康,目前主要采用大剂量抗生素来治疗病猪,但易产生副作用。筛选适宜中药来治疗猪呼吸系统疾病是养猪业发展的迫切需求。本研究将中药诃子、余甘子用中性去离子水索氏提取4~6 h,螃蟹甲、多刺绿绒蒿用无水乙醇索氏提取6 h,蒸干溶剂,收率分别为10.0%、10.5%、11.0%和10.0%。将诃子、余甘子用水稀释,螃蟹甲、多刺绿绒蒿用5%吐温80溶液稀释,得到药物储存液,采用试管法测定诃子及其与其他中药组合对猪呼吸系统疾病病原菌(A族乙型溶血性链球菌、猪链球菌、波氏杆菌、多杀性巴氏杆菌及副猪嗜血杆菌)的最低抑菌浓度。结果显示,诃子对猪呼吸系统疾病病原菌有较强的抑菌作用,余甘子、螃蟹甲、多刺绿绒蒿与诃子的多种药物组合也有较强的抑菌作用,大多数能协同诃子抑制猪呼吸系统疾病常见病原菌,其作用强弱顺序为诃子/余甘子/多刺绿绒蒿(3∶1∶1)>诃子/余甘子/螃蟹甲(3∶1∶1)>诃子/螃蟹甲(2∶1)>诃子>诃子/多刺绿绒蒿(2∶1)>诃子/余甘子(1∶1)。这些药物组合可为猪呼吸系统疾病防治药物的研发提供参考。

关键词：诃子猪呼吸系统疾病病原菌体外抑菌作用

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Investigating Müller glia reprogramming in mice: a retrospective of the last decade, and a look to the future

引用

Neural Regeneration Research 2025年第4期20卷 946-959页

作者： Zhiyuan Yin Jiahui kang Xuan Cheng Hui Gao Shujia Huo Haiwei Xu Key Lab of Visual Damage and Regeneration&Restoration of Chongqing Southwest Eye HospitalSouthwest HospitalThird Military Medical University(Army Medical University)ChongqingChina

Müller glia,as prominent glial cells within the retina,plays a significant role in maintaining retinal homeostasis in both healthy and diseased *** lower vertebrates like zebrafish,these cells assume responsibility for spontaneous retinal regeneration,wherein endogenous Müller glia undergo proliferation,transform into Müller glia-derived progenitor cells,and subsequently regenerate the entire retina with restored ***,Müller glia in the mouse and human retina exhibit limited neural reprogramming.Müller glia reprogramming is thus a promising strategy for treating neurodegenerative ocular disorders.Müller glia reprogramming in mice has been accomplished with remarkable success,through various *** in molecular,genetic,epigenetic,morphological,and physiological evaluations have made it easier to document and investigate the Müller glia programming process in ***,there remain issues that hinder improving reprogramming efficiency and ***,understanding the reprogramming mechanism is crucial toward exploring factors that will improve Müller glia reprogramming efficiency,and for developing novel Müller glia reprogramming *** review describes recent progress in relatively successful Müller glia reprogramming *** also provides a basis for developing new Müller glia reprogramming strategies in mice,including epigenetic remodeling,metabolic modulation,immune regulation,chemical small-molecules regulation,extracellular matrix remodeling,and cell-cell fusion,to achieve Müller glia reprogramming in mice.

关键词： cell fusion chemical small-molecules epigenetic extracellular matrix immune metabolic mice Müller glia neurodegenerative diseases reprogramming retina regeneration

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Potential role of tanycyte-derived neurogenesis in Alzheimer's disease

引用

Neural Regeneration Research 2025年第6期20卷 1599-1612页

作者： Guibo Qi Han Tang Jianian Hu Siying kang Song Qin Department of Anatomy Histologyand EmbryologySchool of Basic Medical SciencesFudan UniversityShanghaiChina State Key Laboratory of Medical Neurobiology and MOE Frontiers Center for Brain Science Fudan UniversityShanghaiChina

Tanycytes, specialized ependymal cells located in the hypothalamus, play a crucial role in the generation of new neurons that contribute to the neural circuits responsible for regulating the systemic energy balance. The precise coordination of the gene networks controlling neurogenesis in naive and mature tanycytes is essential for maintaining homeostasis in adulthood. However, our understanding of the molecular mechanisms and signaling pathways that govern the proliferation and differentiation of tanycytes into neurons remains limited. This article aims to review the recent advancements in research into the mechanisms and functions of tanycyte-derived neurogenesis. Studies employing lineage-tracing techniques have revealed that the neurogenesis specifically originating from tanycytes in the hypothalamus has a compensatory role in neuronal loss and helps maintain energy homeostasis during metabolic diseases. Intriguingly,metabolic disorders are considered early biomarkers of Alzheimer's disease. Furthermore,the neurogenic potential of tanycytes and the state of newborn neurons derived from tanycytes heavily depend on the maintenance of mild microenvironments, which may be disrupted in Alzheimer's disease due to the impaired blood–brain barrier ***, the specific alterations and regulatory mechanisms governing tanycyte-derived neurogenesis in Alzheimer's disease remain unclear. Accumulating evidence suggests that tanycyte-derived neurogenesis might be impaired in Alzheimer's disease, exacerbating neurodegeneration. Confirming this hypothesis, however, poses a challenge because of the lack of long-term tracing and nucleus-specific analyses of newborn neurons in the hypothalamus of patients with Alzheimer's disease. Further research into the molecular mechanisms underlying tanycyte-derived neurogenesis holds promise for identifying small molecules capable of restoring tanycyte proliferation in neurodegenerative diseases. This line of investigation could pro

关键词： Alzheimer's disease blood–brain barrier ependymoglial cells hypothalamus metabolic diseases neural stem cells neurogenesis neuroinflammatory diseases neurons tanycyte

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

8株分离自四川、贵州地区猪繁殖与呼吸综合征病毒毒株的全基因组特征分析

引用

畜牧兽医学报 2020年第6期51卷 1382-1390页

作者：周泷康润敏张毅谢波于吉锋李春张斌汤承王红宁西南民族大学生命科学与技术学院成都610041 四川省畜牧科学研究院成都610066 四川省动物疫病预防控制中心成都610041 成都正大农牧食品有限公司成都610081 四川大学生命科学学院成都610064

本研究旨在了解近年来我国四川、贵州地区猪繁殖与呼吸综合征病毒(PRRSV)的分子流行病学及遗传变异情况,对该地区2016-2018年间采集的8份PRRSV阳性样品进行病毒分离鉴定和全基因组测序,进一步使用RDP4和Simplot生物软件对全基因序列进... 详细信息

本研究旨在了解近年来我国四川、贵州地区猪繁殖与呼吸综合征病毒(PRRSV)的分子流行病学及遗传变异情况,对该地区2016-2018年间采集的8份PRRSV阳性样品进行病毒分离鉴定和全基因组测序,进一步使用RDP4和Simplot生物软件对全基因序列进行重组分析。结果显示,8个PRRSV毒株全基组全长为15010~15321 nt,毒株间相似性为80.9%~91.7%。NSP2氨基酸分析结果显示,4个毒株表现出与HP-PRRSV毒株一致的30 aa缺失,另外4株表现出与NADC30毒株一致的131 aa缺失。其中,GZgy17表现为"1+19+29 aa"新型缺失模式。ORF5氨基酸分析显示,3个毒株在33位点出现新型的1 aa缺失。遗传重组分析显示,8个毒株表现出PRRSV-2毒株6种不同谱系(Lineage)间的重组方式,分别如下:1)SCnj16:L8+L1;2)SCxyz17:L1+L5;3)SCya18:L1+L3;4)GZgy17:L8+L3;5)SCcd17和SCN17:L1+L8+L5;6)SCcd16和SCya17:L1+L8+L3。本研究结果表明,我国四川、贵州地区不仅有多种谱系PRRSV毒株并存,其基因组还出现了复杂的遗传重组现象,具有上述复杂基因组的PRRSV毒株在我国的流行状况值得高度关注。

关键词：猪繁殖与呼吸综合征病毒病毒分离鉴定全基因组缺失重组

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

规模化猪场不同通风模式下不同类型猪舍内空气细菌气溶胶分布规律的研究

引用

畜牧与兽医 2017年第8期49卷 111-116页

作者：康润敏雷云峰曾凯何志平应三成龚建军四川省畜牧科学研究院/动物遗传育种四川省重点实验室四川成都610066

为了研究自然通风模式下和"湿帘-风机系统"下妊娠舍和产仔舍空气细菌气溶胶的分布规律,筛选出更适合养猪生产的养殖模式,采用Andersen-6级撞击式空气微生物采样器对2种通风模式下的妊娠舍和产仔舍15个采样点细菌气溶胶的浓度和粒子分布... 详细信息

为了研究自然通风模式下和"湿帘-风机系统"下妊娠舍和产仔舍空气细菌气溶胶的分布规律,筛选出更适合养猪生产的养殖模式,采用Andersen-6级撞击式空气微生物采样器对2种通风模式下的妊娠舍和产仔舍15个采样点细菌气溶胶的浓度和粒子分布规律进行了比较分析。结果显示:2种通风模式下产仔舍的细菌气溶胶浓度均显著低于妊娠舍(P0.05);细菌气溶胶粒子均主要分布在>7.0μm和2.1~3.3μm区间,通风模式和猪舍类型对细菌气溶胶粒子的分布无显著相关(P>0.05)。研究表明,"湿帘-风机系统"可以显著降低妊娠舍细菌气溶胶的浓度。

关键词：自然通风湿帘-风机系统细菌气溶胶气溶胶粒子分布

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Photo‑Energized MoS_(2)/CNT Cathode for High‑Performance Li–CO_(2)Batteries in a Wide‑Temperature Range

引用

Nano-Micro Letters 2025年第1期17卷 160-175页

作者： Tingsong Hu Wenyi Lian kang Hu Qiuju Li Xueliang Cui Tengyu Yao Laifa Shen Jiangsu Key Laboratory of Materials and Technologies for Energy Storage College of Materials Science and TechnologyNanjing University of Aeronautics and AstronauticsNanjing 210016People’s Republic of China

Li–CO_(2) batteries are considered promising energy storage systems in extreme environments such as Mars;however,severe performance degradation will occur at a subzero temperature owning to the sluggish reaction ***,a photo-energized strategy adopting sustainable solar energy in wide working temperature range Li–CO_(2) battery was achieved with a binder-free MoS_(2)/carbon nanotube(CNT)photo-electrode as *** unique layered structure and excellent photoelectric properties of MoS_(2) facilitate the abundant generation and rapid transfer of photo-excited carriers,which accelerate the CO_(2) reduction and Li_(2)CO_(3) decomposition upon *** illuminated battery at room temperature exhibited high discharge voltage of 2.95 V and mitigated charge voltage of 3.27 V,attaining superior energy efficiency of 90.2%and excellent cycling stability of over 120 *** at an extremely low temperature of−30℃,the battery with same electrolyte can still deliver a small polarization of 0.45 V by the photoelectric and photothermal synergistic mechanism of MoS_(2)/CNT *** work demonstrates the promising potential of the photo-energized wide working temperature range Li–CO_(2) battery in addressing the obstacle of charge overpotential and energy efficiency.

关键词： Li-CO_(2)batteries Photo-energized Wide operation-temperature Kinetics MoS_(2)

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Non-existence of Shimura curves of Mumford type generically in the non-hyperelliptic locus

引用

Science China Mathematics 2025年

作者： Xin Lü Shengli Tan kang Zuo School of Mathematical Sciences Key Laboratory of Mathematics and Engineering Applications(Ministry of Education) Shanghai Key Laboratory of Pure Mathematics and Mathematical PracticeEast China Normal University School of Mathematics and Statistics Wuhan University Institut für Mathematik Universit?t Mainz

We show that there does not exist any Shimura curve with a strictly maximal Higgs field generically in the Torelli locus of non-hyperelliptic curves of genus g≥4. In particular, Shimura curves of Mumford type are not...

关键词：

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：