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A novel unsupervised approach for multilevel image clustering from unordered image collection

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Frontiers of Computer Science 2013年第1期7卷 69-82页

作者： lai kang Lingda WU Yee-Hong YANG College of Information System and Management National University of Defense Technology Changsha 410073 China The Key Lab the Academy of Equipment Command & Technology Beijing 101400 China Department of Computing Science University of Alberta Edmonton Alberta T6G 2E8 Canada

A novel unsupervised approach to automatically constructing multilevel image clusters from unordered im- ages is proposed in this paper. The whole input image col- lection is represented as an imaging sample space （ISS） con- sisting of globally indexed image features extracted by a new efficient multi^view image feature matching method. By mak- ing an analogy between image capturing and observation of ISS, each image is represented as a binary sequence, in which each bit indicates the visibility of a corresponding feature. Based on information theory-inspired image popularity and dissimilarity measures, we show that the image content and distance can be quantitatively described, guided by which an input image collection is organized into multilevel clusters automatically. The effectiveness and the efficiency of the pro- posed approach are demonstrated using three real image col- lections and promising results were obtained from both qual- itative and quantitative evaluation.

关键词： multilevel image clustering imaging sample space (ISS) unordered image collection

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痛泻要方治疗胃肠道疾病研究进展

中国药业 2024年第9期33卷 153-156,I0001页

作者：赖康安李亮胡莹广州中医药大学附属中山中医院广东中山528400

目的为痛泻要方治疗胃肠道疾病的临床应用提供参考。方法采用计算机检索中国知网和PubMed数据库中1992年1月至2023年12月痛泻要方治疗胃肠道疾病的相关文献,分析痛泻要方治疗胃肠道疾病的现代药理学、临床应用及作用机制。结果痛泻要方... 详细信息

目的为痛泻要方治疗胃肠道疾病的临床应用提供参考。方法采用计算机检索中国知网和PubMed数据库中1992年1月至2023年12月痛泻要方治疗胃肠道疾病的相关文献,分析痛泻要方治疗胃肠道疾病的现代药理学、临床应用及作用机制。结果痛泻要方具有补脾柔肝、祛湿止泻功效,是治疗痛泻的首选方剂。临床广泛用于治疗溃疡性结肠炎、慢性结肠炎、肠易激综合征等胃肠道疾病,且疗效和安全性均较好。主要通过保护胃肠道黏膜、调节肠道菌群、抗炎、改善胃肠道动力、缓解焦虑和抑郁情绪等多种途径来改善痛泻症状。结论痛泻要方治疗溃疡性结肠炎等胃肠道疾病的疗效确切,安全性好,但其有效成分、作用机制还有待进一步研究。

关键词：痛泻要方胃肠道疾病溃疡性结肠炎药理学临床应用作用机制

法医学DNA遗传标记和DNA甲基化分子标记的集成检验技术

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生物化学与生物物理进展 2024年第9期51卷 2156-2167页

作者：易娜赵光斌康克莱姚伊人郭柯利赵杰张驰缪磊王乐季安全昆明医科大学法医学院昆明650500 公安部鉴定中心法医遗传学公安部重点实验室北京100038

DNA遗传标记在法医学领域的个体识别、亲缘关系鉴定、始祖推断和表型特征刻画等方面具有重要作用。DNA甲基化分子标记在法医学领域的生物学年龄推断、组织体液鉴定和表型预测等方面具有独特优势。目前的研究多是在不同的检验流程中对DN... 详细信息

DNA遗传标记在法医学领域的个体识别、亲缘关系鉴定、始祖推断和表型特征刻画等方面具有重要作用。DNA甲基化分子标记在法医学领域的生物学年龄推断、组织体液鉴定和表型预测等方面具有独特优势。目前的研究多是在不同的检验流程中对DNA甲基化分子标记和DNA遗传标记独立地进行检验,通过不同的分析流程分别解读这两种标记所蕴含的生物信息。用一份生物检材通过一次检测同时得到DNA遗传信息和表观遗传信息的集成检验方法,能够在获得丰富遗传数据的基础上有效关联这两种标记的生物学信息,节省检验时间,降低检验成本和简化实验流程。本文分别介绍了基于毛细管电泳平台、焦磷酸测序平台和二代测序平台进行DNA遗传标记和DNA甲基化分子标记集成检验的多种方案,分析了各方案在不同平台的技术特点,归纳了各方案的集成化程度和实际应用能力,重点分析了在其他领域中基于二代测序技术进行集成检验的技术路线,以期挖掘其在法医学生物检材中的应用潜力,探讨了位点间相互作用和甲基化预处理方法对DNA遗传标记和DNA甲基化分子标记集成检验的影响,提出了在法医学生物检材中应用集成检验技术所面临的挑战,期望为法医学研究实践提供参考。

关键词：法医遗传学 DNA 甲基化集成检验方法二代测序

Penetration of a Single Domain of Bacillus thuringiensis Cry1Ie-Domain I to a Lipid Membrane In vitro

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Journal of Integrative Agriculture 2014年第5期13卷 1043-1050页

作者： GUO Shu-yuan LI Jie CHEN Zhen HE kang-lai School of Life Science Beijing Institute of Technology State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection Chinese Academy of Agricultural Sciences

Domain I of the activated Crystal protein from Bacillus thuringiensis has a seven a-helix bundle structure, which is responsible for membrane channel formation in its insecticidal mechanism. Crylle is toxic to Asian corn borer, Ostrinia furnacalis （Guen6e）, and plays important roles in insect biological control. The domain I from Crylle has been expressed and purified in its normal conformation, as embedded in the full length homologous toxin structure. The membrane insertion ability of this single domain was compared with the full length homologous toxin using a monolayer insertion experiment. The results indicated that the Crylle-domain I had the ability to insert into the lipid monolayer, and this ability is greater than that of the IE648 toxin. However, the state of insertion is not stable and remains for only a short period of time. The Crylle-domain I plays no role in receptor binding as it had a nonspecific binding with the brush border membrane vesicles of the Asian corn borer.

关键词： Bacillus thuringiensis Cryl Ie domain I of Cry protein monolayer insertion

An Improved BP Algorithm and Its Application in Classification of Surface Defects of Steel Plate

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Journal of Iron and Steel Research International 2007年第2期14卷 52-55页

作者： ZHAO Xiang-yang lai kang-sheng DAI Dong-ming Research and Development Division Haler Group Qingdao 266101 Shandong China Department of Physics Dalian University of Technology Dalian 116024 Liaoning China

Artificial neural network is a new approach to pattern recognition and classification. The model of multilayer perceptron （MLP） and back-propagation （BP） is used to train the algorithm in the artificial neural network. An improved fast algorithm of the BP network was presented, which adopts a singular value decomposition （SVD） and a generalized inverse matrix. It not only increases the speed of network learning but also achieves a satisfying precision. The simulation and experiment results show the effect of improvement of BP algorithm on the classification of the surface defects of steel plate.

关键词： artificial neural network MLP BP algorithm SVD generalized inverse matrix

Applications of structure from motion: a survey

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Journal of Zhejiang University-Science C(Computers and Electronics) 2013年第7期14卷 486-494页

作者： Ying-mei WEI lai kang Bing YANG Ling-da WU College of Information System and Management National University of Defense Technology National Laboratory of Electronic Information Equipment System Academy of Equipment Command and Technology

Structure from motion (SfM) has been an active research area in computer vision for decades and numerous practical applications are benefiting from this research. While no previous work has tried to summarize the applications appearing in the literature, this paper deals with a comprehensive overview of recent applications of SfM by classifying them into 10 categories, namely augmented reality, autonomous navigation/guidance, motion capture, hand-eye calibration, image/video processing, image-based 3D modeling, remote sensing, image organization/browsing, segmentation and recognition, and military applications. The goal is to provide insights for researchers to position their work more appropriately in the context of existing techniques, and to perceive both new applications and relevant research problems.

关键词： Structure from motion (SfM) Image-based 3D modeling Application

Wolbachia Infection in Populations of Ostrinia furnacalis: Diversity, Prevalence, Phylogeny and Evidence for Horizontal Transmission

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Journal of Integrative Agriculture 2013年第2期12卷 283-295页

作者： LI Jing WANG Zhen-ying Denis Bourguet HE kang-lai State Key Laboratory for the Biology of the Plant Diseases and Insect Pests/Institute of Plant Protection Chinese Academy of Agricultural Sciences Centre de Biologie pour la Gestion des Populations (CBGP) UMR INRA-IRD-CIRAD-Montpellier SupAgro Campus International de Baillarguet

Wolbachia are maternally inherited endosymbiotic bacteria infecting a wide range of arthropods and filarial nematodes. They can induce various reproduction alterations in their hosts, including thelytokous parthenogenesis, cytoplasmic incompatibility （CI）, feminization of genetic males and male killing. Here we investigated diversity and prevalence patterns of Wolbachia infection in 43 geographical populations of the Asian corn borer, Ostriniafurnacalis, in China and one population in North Korea. Based on Wolbachia surface protein gene （wsp） sequences, nine strains of Wolbachia （wFurl-wFur9）, belonging to supergroups A and B, were identified in populations of O. furnacalis with an average infection rate of 10.5%. Superinfection commonly appeared in individuals of O. furnacalis and coinfection patterns were very complex. There was no specific pattern for the prevalence and distribution of the nine Wolbachia strains suggesting an intricate evolutionary history of Wolbachia infection in this species. The genetic similarity of the wFurl-wFur9 strains with those detected in two parasitoids of O. furnacalis, Macrocentrus cingulum and Lydella grisescens, strongly suggests host-parasitoid horizontal transmission.

关键词： Wolbachia Ostriniafurnacalis infection diversity prevalence pattern horizontal transmission

江苏常熟地区汉族人群47个微单倍型的遗传多态性及遗传结构分析

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生物化学与生物物理进展 2024年第2期51卷 423-434页

作者：潘坤鹏冯耀森于文帅刘宗伟姚伊人赵杰康克莱张驰王乐吴坚昆明医科大学法医学院昆明650500 公安部鉴定中心法医遗传学公安部重点实验室现场物证溯源技术国家工程实验室北京100038 中国人民公安大学侦查学院北京100038 江苏省常熟市公安局常熟215500

目的探索江苏常熟地区汉族人群在47个常染色体微单倍型基因座的遗传多态性,评估应用效能及法庭科学参数。方法采用MHSeqTyper47混合DNA鉴定试剂盒进行基因座复合扩增及文库构建,使用MiSeq FGx测序平台进行测序,测得的数据应用MHTyper数... 详细信息

目的探索江苏常熟地区汉族人群在47个常染色体微单倍型基因座的遗传多态性,评估应用效能及法庭科学参数。方法采用MHSeqTyper47混合DNA鉴定试剂盒进行基因座复合扩增及文库构建,使用MiSeq FGx测序平台进行测序,测得的数据应用MHTyper数据分析软件进行分析,对获得的样本遗传信息进行评估,结合千人基因组数据(1000 Genomes Project phase 3,1KG)评估群体间遗传分化指数及遗传距离,并计算法庭科学参数。结果江苏常熟地区汉族人群与1KG中的中国北京群体的遗传分化和遗传距离最小,并得到最接近的有效等位基因数(Ae),累积随机匹配概率(combined matching probability,CMP)与1KG中东亚参考人群的5个群体均接近,为1.25×10^(-36),累积非父排除概率达0.9999999999641。结论本研究报告了47个微单倍型基因座在江苏常熟地区汉族人群中的等位基因频率及遗传多态性信息,为47个微单倍型在法医学应用中提供了数据基础。另外,比较了1KG参考人群与江苏常熟地区汉族人群的多态性差异,并揭示了47个微单倍型在江苏常熟地区汉族人群中的遗传结构。总的来说,1KG中的东亚人群参考数据更符合江苏常熟地区汉族人群的遗传特征。

关键词：法医遗传学微单倍型二代测序

Effects of photoperiod and temperature on diapause induction in Conogethes punctiferalis （Lepidoptera： Pyralidae）

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Insect Science 2014年第5期21卷 556-563页

作者： Li-Rong Xu Xinzhi Ni Zhen-Ying Wang kang-lai He The State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant Protection Chinese Academy of Agricultural Sciences China USDA-ARS Crop Genetics and Breeding Research Unit University of Georgia-Tifton Campus Tifton GeorgiaUSA

The yellow peach moth, Conogethes punctiferalis （Guenee）, a multivoltine species that overwinters as diapausing larvae, is one of the most serious insect pests on maize in China. Effect of photoperiod and temperature on larval diapause was examined under empirical laboratory conditions. Short-day treatments caused larval diapause at 25℃, and the critical photoperiod was between 12 and 13 h （or 12 h 51 min） light per day. No sensitive instar was identified for diapause induction under alternated short- （L ： D 11 ： 13 h） and long-day （L ： D 14 ： 10 h） treatments at different larval stages. However, accumulative treatment of three instars and 10 d under short-day treatment was required for the induction of 50% larval diapause. All larvae entered diapause at 20℃, whereas less than 3% did so at 30℃, irrespective of the long- or short-day treatment. Furthermore, under the short-day treatment, more than 90% of larvae went into diapause with temperatures ≤ 25℃, but less than 17% did so at 28℃. In contrast, under the long-day treatment, less than 19% of larvae went into diapause with temperatures ≥23 ℃. The forward shift （5℃） of critical temperature under the long-day regime demonstrated the compensatory effect of temperature and photoperiod on diapause induction. In conclusion, C. punctiferalis had a temperature-dependent type Ⅰ photoperiodic diapause response; there was no sensitive instar for diapause determination, but the photoperiodic accumulation time countermeasures both of the short-day cycles and the number ofinstars exposed, and the photoperiodic diapause response, was a temperature-compensated phenomenon.

关键词： Conogethes punctiferalis diapause induction photoperiod sensitive larval instar temperature dependence yellow peach moth