检索结果-南通市图书馆

The identities and anti-herpes simplex virus activity of Clinacanthus nutans and Clinacanthus siamensis

Asian Pacific Journal of Tropical Biomedicine 2013年第4期3卷 284-290页

作者： Paween Kunsorn Nijsiri Ruangrungsi Vimolmas Lipipun Ariya Khanboon kanchana rungsihirunrat College of Public Health Sciences Chulalongkorn UniversityBangkok 10330Thailand Faculty of Pharmaceutical Sciences Chulalongkorn UniversityBangkok 10330Thailand

Objective:To distinguish the difference among the Clinacanthus nutans(Burm.f.)Lindau(***)and Clinacanthus siamensis Bremek(***)by assessing pharmacognosy characteristics,molecular aspect and also to evaluate their anti-herpes simplex virus(HSV)type 1 and type 2 ***:Macroscopic and microscopic evaluation were performed according to WHO Geneva *** number,stomatal index and palisade ratio of leaves were *** DNA was extracted by modified CTAB method and ITS region was amplified using PGR and then *** leaves were subsequently extracted with n-hexane,dichloromethane and methanol and antiviral activity was performed using plaque reduction assay and the cytotoxicity of the extracts on Vero cells was determined by MTT ***:Cross section of midrib and stem showed similar major *** measurement index of stomatal number,stomatal index and palisade ratio of *** were 168.32±29.49,13.83±0.86 and 6.84±0.66,respectively,while *** were 161.60±18.04,11.93±0.81and 3.37±0.31,*** PCR amplification of ITS region generated the PGR product approximately 700 bp in *** were 34 polymorphisms within the ITS region which consisted of 11 Indels and 23 nucleotide *** IC_(50)values of *** extracted with n-hexane,dichloromethane and methanol against HSV-1 were(32.05±3.63)μg/mL,(44.50±2.66)μg/mL,(64.93±7.00)μg/mL,respectively where as those of *** were(60.00±11.61)μg/mL,(55.69+4.41)μg/mL,(37.39±5.85)μg/mL,*** HSV-2 activity of n-hexane,dichloromethane and methanol *** leaves extracts were(72.62±12.60)μg/mL,(65.19±21.45)μg/mL,(65.13±2.22)μg/mL,respectively where as those of *** were(46.52±4.08)μg/mL,(49.63±2.59)μg/mL,(72.64±6.52)μg/mL,***:The combination of macroscopic,microscopic and biomolecular method are able to authenticate these closely related plants and both of them have a potency to be an anti-HSV age

关键词： Clinacanthus nutans Clinacanthus siamensis Microscopic analysis Biomolecular analysis Internal transcribed spacer Herpes simplex virus Plaque reduction assay

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Assessment of in vitro sensitivity of Plasmodium vivax fresh isolates

引用

Asian Pacific Journal of Tropical Biomedicine 2011年第1期1卷 49-53页

作者： Poonuch Muhamad Wanna Chacharoenkul kanchana rungsihirunrat Ronnatrai Ruengweerayut Kesara NaBangchang Pharmacology and Toxicology Unit Graduate Program in Biomedical SciencesThammasat University College of Public Health Chulalongkorn University Mae-Sot General Hospital

Objective:To compare the applicability of the SYBK Grcen-Ⅰ assay with the standard schizont maturalion assay,for determination of sensitivity of Plasmodium vivax(***) to chloroquine and a new antifolale WR ***:The study was conducted at Mae Tao Clinic for migrant workers,Tak Province during April 2009 to July 2010.A total of 64 blood samples(1 mL blood collected into sodium heparinized plastic tube) were collected from patients with monoinfection with *** malaria prior to treatment with standard regimen of a 3-day chloroquine. In vitro sensitivity of *** isolates was evaluated by schizont maturation inhibition and SYBR Green-Ⅰ ***:A total of 30 out of 64 blood samples collected from patients with *** malaria were successfully analyzed using both the microscopic schizont maturation inhibition and SYBR Green-I *** failure rates of the schizont maturation inhibition assay(50%) and the SYBR Green-I assay(54%) were similar(P=0.51).The median IC_(10)s,IC_(50)s and IC_(90)s of both chloroquine and WR99210 were not significantly different from the clinical isolates of *** *** on the cut-off of 100 nM,the prevalences of chloroquine resistance determined by schizont maturation inhibition and SYBR Green-I assays were 19 and 11 isolates,*** strength of agreement between the two methods was very poor for both chloroquine and ***:On the basis of this condition and its superior sensitivity,the microscopic method appears better than the SYUK Green-I Green assay for assessing in vitro sensitivity of fresh *** isolates to antimalarial drugs.

关键词： Plasmodium vivax Chloroquine In vitro sensitivity Schizont maturation inhibition assay SYBR Green-Ⅰassay Fresh isolate Antifolate Malaria Antimalarial drug Standard regimen

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Proteomics analysis of antimalarial targets of Garcinia mangostana Linn.

引用

Asian Pacific Journal of Tropical Biomedicine 2014年第7期4卷 515-519页

作者： Wanna Chaijaroenkul Artitiya Thiengsusuk kanchana rungsihirunrat Stephen Andrew Ward Kesara Na-Bangchang Chulabhorn International College of Medicine.Thammasat University(Rangsit Campus) College of Public Health Sciences Chulalongkorn University Molecular and Biochemical Parasitology.Liverpool School of Tropical Medicine University of Liverpool

Objective:To investigate possible protein targets for antimalarial activity of Garcina mangostana Linn.(***)(pericarp)in 3D7 Plasmodium falciparum clone using 2-dimensional electrophoresis and liquid chromatography mass-spectrometry(LC/MS/MS).Methods:3D7 Plasmodium falciparum was exposed to the crude ethanolic extract of *** Linn.(pericarp)at the concentrations of 12μg/mL(1C_(50)level:concentration that inhibits parasite growth by 50%)and 30μg/mL(1C_(90)level:concentration that inhibits parasite growth by 90%)for 12 *** proteins were separated by 2-dimensional electrophoresis and identified by LC/MS/***:At the IC_(50)concentration,about 82%of the expressed parasite proteins were matched with the control(non-exposed),while at the IC_(90)concentration,only 15%matched proteins were *** selected protein spots from parasite exposed to the plant extract at the concentration of 12μg/mL were identified as eneymes that play role in glycolysis pathway,i.e.,phosphoglyeerate mutase putative,L-lactate dehydrogenase/glyceraldehyde-3-phosphate dehydrogenase,and fruetose-bisphosphate aldolase/phosphoglyeerate *** proteosome was found in parasite exposed to 30μg/mL of the ***:Results suggest that proteins involved in the glycolysis pathway may be the targets for antimalarial activity of *** Linn.(pericarp).

关键词： Malaria Proteomics Garcinia mangostana Linn.

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Comparison of protein patterns between Plasmodium falciparum mutant clone T9/94-M1-1(b3) induced by pyrimethamine and the original parent clone T9/94

引用

Asian Pacific Journal of Tropical Biomedicine 2012年第1期2卷 66-69页

作者： kanchana rungsihirunrat Wanna Chaijaroenkul Napapom Siripoon Aree Seugorn Sodsri Thaithong Kesara Na-Bangchang College of Public Health Sciences Chulalongkorn University Graduate Program in Biomedical Sciences Thammasat University

Objective:To compare the protein patterns from the extracts of the mutant clone T9/94-M1-1(b3)induced by pyrimethamine,and the original parent clone T9/94 following separation of parasite extracts by two-dimensional electrophoresis(2-DE).Methods:Proteins were solubilized and separated according to their charges and *** separated protein spots were then detected by silver staining and analyzed for protein density by the powerful image analysis ***:Differentially expressed protein patterns(up—or down-regulation)were separated from the extracts from the two clones.A total of 223 and 134 protein spots were detected from the extracts of T9/94 and T9/94-M1-1(b3)clones,*** reduction in density of protein expression was observed with the extract from the mutant(resistant)clone compared with the parent(sensitive)clone.A total of 25 protein spots showed at least two-fold difference in density,some of which exhibited as high as ten-fold ***:These proteins may be the molecular targets of resistance of Plasmodium falciparum to *** study to identify the chemical structures of these proteins by mass spectrometry is required.

关键词： Plasmodium falciparum Proteomics Pyrimethamine Drug resistance Protein Molecular target

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：