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Study of ss-DNA Adsorption and Nano-mechanical Properties on Mica Substrate with Surface Forces Apparatus

Chinese Journal of Mechanical Engineering 2018年第5期31卷 91-98页

作者： Gui-Bin Shen Ya-jing kan Min-Hua Chen Yun-Fei Chen Jiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments Southeast University Nanjing 211189 China School of Mechanical Engineering Southeast University Nanjing 211189 China

Many DNA?based devices need to build stable and controllable DNA films on surfaces. However, the most com?monly used method of film characterization, namely, the probe?like microscopes which may destroy the sample and substrate. Surface Forces Apparatus(SFA) technique, specializing in surface interaction studies, is introduced to investigate the e ects of DNA concentration on the formation of single?stranded DNA(ss?DNA) film. The result demonstrates that 50 ng/μL is the lowest concentration that ss?DNA construct a dense layer on mica. Besides, it is also indicated that at di erent DNA concentrations, ss?DNA exhibit diverse morphology: lying flat on surface at 50 ng/μL while forming bilayer or cross?link at 100 ng/μL, and these ss?DNA structures are stable enough due to the repeatabil?ity even under the load of 15 mN/m. At the same time, an obvious adhesion force is measured:/m at 100 ng/μL, respectively, which is attributed to the ion?correlation e ect. M-6.5 mN/m at 50 ng/μL and-5.3 mNoreover, the atomic force microscopy(AFM) images reveal the entire surface is covered with wormlike ss?DNA and the measured surface roughness(1.8±0.2 nm) also matches well with the film thickness by SFA. The desorption behaviors of ss?DNA layer from mica surface occur by adding sodium salt into gap bu er, which is mainly ascribed to the decreased ion?ion cor?relation force. This paper employing SFA and AFM techniques to characterize the DNA film with flexibility and stable mechanical ability achieved by ion bridging method, is helpful to fabricate the DNA?based devices in nanoscale.

关键词： Single?stranded DNA Adsorption Nano?mechanical properties Ion?correlation effect Surface forces apparatus

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The Response Difference of Mitochondria in Recalcitrant Antiaris toxicaria Axes and Orthodox Zea mays Embryos to Dehydration Injury

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Journal of Integrative Plant Biology 2009年第7期51卷 646-653页

作者： Song-Quan Song Mei-Hua Tian jing kan Hong-Yan Cheng Institute of Botany the Chinese Academy of Sciences Beijing 100093 China Xishuangbanna Tropical Botanical Garden the Chinese Academy of Sciences Mengla 666303 China

Long-term preservation of recalcitrant seeds is very difficult because the physiological basis on their desiccation sensitivity is poorly understood. Survival of Antiaris toxicaria axes rapidly decreased and that of immature maize embryos very slowly decreased with dehydration. To understand their different responses to dehydration, we examined the changes in mitochondria activity during dehydration. Although activities of cytochrome （Cyt） c oxidase and malate dehydrogenase of the A. toxicaria axis and maize embryo mitochondria decreased with dehydration, the parameters of maize embryo mitochondria were much higher than those of A. toxicaria, showing that the damage was more severe for the A. toxicaria axis mitochondria than for those of maize embryo. The state I and III respiration of the A. toxicaria axis mitochondria were higher than those of maize embryo, the former rapidly decreased, and the latter slowly decreased with dehydration. The proportion of Cyt c pathway to state III respiration for the A. toxicaria axis mitochondria was low and rapidly decreased with dehydration, and the proportion of alternative oxidase pathway was high and slightly increased with dehydration. In contrast, the proportion of Cyt c pathway for maize embryo mitochondria was high, and that of alternative oxidase pathway was low. Both pathways decreased slowly with dehydration.

关键词： alternative oxidase pathway cytochrome c oxidase pathway dehydration mitochondria orthodox recalcitrance respiration rate seed.

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Nuclear receptors modulate inflammasomes in the pathophysiology and treatment of major depressive disorder

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World Journal of Psychiatry 2021年第12期11卷 1191-1205页

作者： Han Wang Wei-jing kan Yuan Feng Lei Feng Yang Yang Pei Chen jing-Jie Xu Tian-Mei Si Ling Zhang Gang Wang jing Du The National Clinical Research Center for Mental Disorders&Beijing Key Laboratory of Mental Disorders Beijing Anding HospitalBeijing 100088Beijing ProvinceChina Department of Clinical Psychopharmacology Peking University Institute of Mental HealthBeijing 100191Beijing ProvinceChina State Key Laboratory for Conservation and Utilization of Bio-resources in Yunnan Yunnan UniversityKunming 650091Yunnan ProvinceChina

Major depressive disorder(MDD)is highly prevalent and is a significant cause of mortality and morbidity ***,conventional pharmacological treatments for MDD produce temporary remission in<50%of patients;therefore,there is an urgent need for a wider spectrum of novel antidepressants to target newly discovered underlying disease *** evidence has shown that immune inflammation,particularly inflammasome activity,plays an important role in the pathophysiology of *** this review,we summarize the evidence on nuclear receptors(NRs),such as glucocorticoid receptor,mineralocorticoid receptor,estrogen receptor,aryl hydrocarbon receptor,and peroxisome proliferator-activated receptor,in modulating the inflammasome activity and depression-associated *** review provides evidence from an endocrine perspective to understand the role of activated NRs in the pathophysiology of MDD,and to provide insight for the discovery of antidepressants with novel mechanisms for this devastating disorder.

关键词： Major depressive disorder Immune inflammation Inflammasome Nuclear receptors

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A novel angiography-based computational modelling for assessing the dynamic stress and quantitative fatigue fracture risk of the coronary stents immediately after implantation: Effects of stent materials, designs and target vessel motions

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Medicine in Novel Technology and Devices 2022年第2期14卷 31-38页

作者： Xinlei Wu Mattia Lunardi Ahmed Elkoumy Jiayue Huang jing kan Shaoliang Chen Shengxian Tu Shanghai Med-X Engineering Research Center Shanghai Jiao Tong UniversityShanghaiChina Institute of Cardiovascular Development and Translational Medicine The Second Affiliated Hospital and Yuying Children's Hospital of Wenzhou Medical UniversityWenzhouZhejiangChina Department of Cardiology National University of IrelandGalway(NUIG)GalwayIreland Division of Cardiology Nanjing First HospitalNanjing Medical UniversityNanjingChina Med-X Research Institute Shanghai Jiao Tong UniversityShanghaiChina

Fluctuating stress on the implanted coronary stents within cardiac cycle is an important mechanism of fatigue fracture,which is associated with in-stent restenosis and stent *** developed a novel computational modelling to calculate the dynamic stress of stents based on the time sequence angiography immediately after *** groups of patient-specific cases(one same stent design treated in 4 different coronary arteries and one same artery actually/virtually implanted one stent with 3 different designs)were performed the dynamic stress analysis by this computational modelling and subsequently assessed the fatigue fracture risk by Goodman *** motion of target arteries significantly impacts on distribution of the stress and the risk of stent fracture,particularly in the site of hinge *** the location of stent stress concentration in the obtuse marginal artery and the“unsafe”region in the inverse fatigue safety factor contour co-registered with the position of complete transverse fracture 13 months later after *** stents with different designs had the same location of highest stress concentration at the hinge motion site of the actually/virtually treated *** strength stent materials are significantly lower the risk of stent fracture rather than stent *** new computational modelling might be a useful tool in assessment of fracture risk of the implanted stent and in optimizing new design of dedicated stent treated specific coronary arteries and mechanical properties in vivo of bioresorbable scaffold during degradation process.

关键词： Coronary angiography Dynamic stress Superficial wall strain Fatigue analysis Cardiac cycle Cardiovascular biomechanics

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Hemodynamic change in wall shear stress in patients with coronary bifurcation lesions treated by double kissing crush or single-stent technique

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Chinese Medical Journal 2012年第10期125卷 1720-1726页

作者： CHEN Shao-liang kan jing ZHANG Jun-jie HU Zuo-ying XU Tian Division of Cardiology Nanjing First HospitalNanjing Medical UniversityNanjingJiangsu 210006China

Background Fluid dynamic mechanisms attributed to coronary bifurcation lesions remain a subject of study. The present study aimed at investigating the hemodynamic change of wall shear stress （WSS） in patients with coronary bifurcation lesions treated by double kissing （DK） crush or one-stent with final kissing balloon inflation （FKBI）. Methods Eighty-one patients with bifurcation lesions treated by stenting who had 3-D model reconstruction were studied. The bifurcation vessels were divided into main vessel （MV）, main branch （MB）, side branch （SB）, and polygon of confluence （POC）. MB and SB were classified by internal- and lateral-subsegments, respectively. Results The baseline magnitude of WSS in proximal MV, POC-MV, POC-MB, POC-SB and MB-internal segments increased significantly, compared to MB-lateral, SB-internal and SB-lateral. DK crush had the potential of uniformly reducing WSS, turbulent index and the WSS gradient. The WSS value at the POC-SB and SB in the one-stent group remained higher. The turbulent index and WSS gradient between the POC-SB minus the SB-lateral had equal predictive values for in-stent restenosis （ISR）. Conclusion Fluid dynamic results favor the use of DK crush over the one-stent technique.

关键词： coronary bifurcation fluid dynamics, shear stress, DK crush

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Impact of Side Branch Lesion Length on Clinical Outcome after Coronary Stenting Techniques in Patients with Coronary Artery Bifurcation Disease:A Meta-Analysis

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Cardiology Discovery 2022年第3期2卷 157-173页

作者： Junyan kan Shuai Luo Dongchen Wang Dandan Cai Xiaojuan Zhang jing kan The First Clinical Medical College Nanjing Medical UniversityNanjingJiangsu 210029China Division of Cardiology Nanjing First HospitalNanjing Medical UniversityNanjingJiangsu 210006China

Objective:The optimal percutaneous coronary intervention(PCI)technique for bifurcation lesions remains controversial,especially considering the variability of the side branch(SB).A provisional stenting technique is currently recommended in most *** meta-analysis aimed to compare outcomes of different bifurcation PCI strategies,clarifying their scope of ***:Randomized controlled trials comparing PCI strategies for coronary bifurcation lesions were systematically retrieved from PubMed,Cochrane,Web of Science,and EBSCO literature databases without limitations on published date or *** adverse cardiovascular events(MACEs)were stipulated as main *** outcomes of interest were all-cause mortality,cardiovascular mortality,target lesion revascularization(TLR),target vessel revascularization,myocardial infarction(MI),and stent *** pooled analysis and sub-group analysis were ***:Twenty-three randomized controlled trials with 6380 participants were *** studies compared the provisional strategy with 2-stent *** significant difference in MACEs(relative risk(RR),1.16;95%confidence interval(CI),0.90-1.48;I2=62%)was found between 1-stent and 2-stent ***,when SB lesion length was used as the separation condition,the 2-stent strategy was associated with fewer MACEs(RR,1.87;95%CI,1.46-2.41;I2=70%),TLRs(RR,2.13;95%CI,1.50-3.02;I2=59%),and MIs(RR,2.17;95%CI,1.19-3.95;I2=52%)than the provisional strategy in those where SB lesions measured>10 mm ***:In the current work,there was no significant difference between 1-stent and 2-stent techniques in terms of MACEs or secondary ***,2-stent approaches have clinical advantages over the provisional strategy in bifurcation when the SB lesion length is>10 mm due to fewer cases of TLR and MI.

关键词： Percutaneous coronary intervention Complex coronary bifurcation lesions Provisional stenting Two-stent strategy Major adverse cardiovascular events Stent thrombosis

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Comparison of two and three dimensional quantitative coronaryangiography to intravascular ultrasound in the assessment of leftmain coronary artery bifurcation lesions

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Chinese Medical Journal 2014年第6期127卷 1012-1021页

作者： kan jing Gao Xiaofei Kumar Gami Sandeep Xu Haimei Zhao Yingying Chen Shaoliang Chen Feng Department of Cardiology Nanjing First Hospital Nanjing Medical University Nanjing Jiangsu 210006 China School of Public Health Nanjing Medical University NanjingJiangsu 210006 China

Background Angiographic evaluation of left main coronary artery(LMCA) bifurcation lesions is often limited. two dimensional(2D) quantitative coronary angiography(QCA) with segmental analysis provides accuracy for quantification of the degree of stenosis in the main vessel and side branch ostium but can be affected by foreshortening and variable magnification. The accuracy of three dimensional(3D) QCA has recently developed to overcome 2D QCA limitations, however, accuracy and precision of 3D bifurcation QCA measurements in LMCA bifurcation lesions has not been *** We investigated whether such 3D and 2D bifurcation QCA measurements differ in their accuracy in assessing significant LMCA bifurcation lesions defined by intravascular ultrasound(IVUS) as a minimum luminal area(MLA) <6 mm2 of LMCA and MLA <4 mm2 of proximal left anterior descending(LAD) and/or proximal left circumflex(LCX)Results LMCA bifurcation lesions were assessed in 44 patients undergoing elective percutaneous coronary intervention. From 2D QCA measurements, MLA correlated moderately with threshold intravascular ultrasound MLA for LMCA(r=0.81, P <0.000 1), LAD(r=0.54, P=0.000 1) and LCX(r=0.58, P <0.000 1). Severity of lesion as MLA by derived 3D QCA, correlated moderately with threshold intravascular ultrasound MLA for LMCA(r=0.84, P <0.000 1), LAD(r=0.53, P=0.000 2); LCX(r=0.66, P <0.000 1). Overall, the C statistics tended to be slightly higher for 3D QCA and 2D QCA measurements in LMCA segment compared with proximal LAD and LCX segments, and there were no significant predictive power of percent diameter stenosis and percent area stenosis on 3D QCA for LCX IVUS MLA <4 mm2(percent diameter stenosis: area under curve 0.55, cutoff 23%, sensitivity 88%, specificity 37%, P=0.618 6; percent arer stenosis: area under curve 0.56, cutoff 41%, sensitivity 83%, specificity 38%, P=0.518 4, respectively).Conclusions The accuracy of 3D bifurcation QCA in detecting significant LMCA bifurcation les

关键词： three dimensional quantitative coronary angiography intravascular ultrasound left main coronary artery

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技术驱动型连续审计模式研究

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中国流通经济 2009年第6期23卷 65-68页

作者：阚京华南京财经大学会计学院江苏南京210003

本文认为,连续审计与传统期间审计的不同之处在于,它是一种技术驱动型审计,具有不间断性和循环性,强调审计实施的即时性,是一种基于异常(例外)事项的审计,可以经济地实现详细测试。文章提出,我国应制定推进连续审计的短期计划和长期规划... 详细信息

本文认为,连续审计与传统期间审计的不同之处在于,它是一种技术驱动型审计,具有不间断性和循环性,强调审计实施的即时性,是一种基于异常(例外)事项的审计,可以经济地实现详细测试。文章提出,我国应制定推进连续审计的短期计划和长期规划,加强连续审计理论研究,注重将连续审计逐渐纳入重要的审计规范和指导中,联合信息技术等相关单位加强连续审计软件开发,加强连续审计人才的培养。

关键词：技术驱动连续审计实时

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强调事项段、审计意见变通和投资者市场反应

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中国流通经济 2008年第7期22卷 52-55页

作者：阚京华南京财经大学会计学院江苏南京210003

本文认为,我国投资者对非限制性强调事项和限制性强调事项的无保留审计意见的市场反应没有明显区别,但是对非限制性强调事项的无保留审计意见有显著的负反应。这说明我国投资者对比较清晰和易于理解的非限制性强调事项能作出判别,而对... 详细信息

本文认为,我国投资者对非限制性强调事项和限制性强调事项的无保留审计意见的市场反应没有明显区别,但是对非限制性强调事项的无保留审计意见有显著的负反应。这说明我国投资者对比较清晰和易于理解的非限制性强调事项能作出判别,而对比较复杂的限制性强调事项缺乏辨别力,在不存在市场和投资者监督的压力下,必然会增加注册会计师利用限制性强调事项改变审计意见的动机,因此有必要通过政府监管手段来规范注册会计师出具带强调事项段的无保留审计意见的行为。在投资者信息识别能力较低的情况下,2003年和2006年审计报告准则对强调事项的内容加以限制是非常及时和必要的,通过对强调事项的严格限制可以约束注册会计师审计报告行为,抑制注册会计师利用强调事项段变通审计意见。

关键词：强调事项无保留意见市场反应制度约束

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IPO询价制度存在的弊端及修补机制

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审计与经济研究 2005年第3期20卷 78-79,83页

作者：阚京华南京财经大学会计学院江苏南京210003

IPO询价制度在对现行的股票市场产生积极推动作用的同时,也带来了一定的问题和弊端。本文着重对IPO询价制度的弊端作出分析,并提出相应的修补机制。

关键词： IPO询价制度修补机制股票市场中国市场推动作用

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