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Successful elimination of extended-spectrum beta-lactamase (ESBl)-producing nosocomial bacteria at a neonatal intensive care unit

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World Journal of Pediatrics 2017年第3期13卷 210-216页

作者： Borbála Szél Zsolt Reiger Edit Urbán Andrea lázár Krisztina Mader Ivelina Damjanova kamilla Nagy Gyula Tálosi Infection Control Unit University of SzegedHungary Department of Pediatrics University of SzegedHungary Institute of Clinical Microbiology University of SzegedHungary Department of Bacteriology National Center for EpidemiologyHungary

Background:Extended-spectrum beta-lactamase (ESBl)-producing Gram-negative bacteria are highly dangerous to neonates.At our Neonatal Intensive Care Unit (NICU),the presence of these bacteria became so threatening in 2011 that immediate intervention was required.Methods:This study was conducted during a nearly two-year period consisting of three phases:retrospective (9 months),educational (3 months) and prospective (9 months).Based on retrospective data analysis,a complex management plan was devised involving the introduction of the INSURE protocol,changes to the antibiotic regimen,microbiological screening at short intervals,progressive feeding,a safer bathing protocol,staff hand hygiene training and continuous monitoring of the number of newly infected and newly colonized patients.During these intervals,a total of 355 patients were monitored.Results:Both ESBl-producing Enterobacter cloaceae and Klebsiella pneumoniae were found (in both patients and environmental samples).In the prospective period a significant reduction could be seen in the average number of both colonized (26/167 patients;P=0.029) and infected (3/167 patients;P=0.033) patients compared to data from the retrospective period regarding colonized (72/188 patients) and infected (9/188 patients) patients.There was a decrease in the average number of patientdays (from 343.72 to 292.44 days per months),though this difference is not significant (P=0.058).During the prospective period,indirect hand hygiene compliance showed a significant increase (from the previous 26.02 to 33.6 hand hygiene procedures per patient per hospital day,Plusion:Colonizations and infections were rolled back successfully in a multi-step effort that required an interdisciplinary approach.

关键词： hand hygiene INSURE protocol interdisciplinary approach neonates polyresistance

Angiogenesis and nerve regeneration induced by local administration of plasmid pBud-coVEGF165-coFGF2 into the intact rat sciatic nerve

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Neural Regeneration Research 2021年第9期16卷 1882-1889页

作者： Ruslan Masgutov Alina Zeinalova Alexey Bogov Galina Masgutova Ilnur Salafutdinov Ekaterina Garanina Valeriia Syromiatnikova kamilla Idrisova Adelya Mullakhmetova Dina Andreeva liliya Mukhametova Adilet Kadyrov Igor Pankov Albert Rizvanov Openlab“Gene and Cell Technologies” Institute of Fundamental Medicine and BiologyKazan Federal UniversityKazanRussia Republican Clinical Hospital KazanRussia Department of Traumatology and Orthopedics Kazan State Medical AcademyKazanRussia

Vascular endothelial growth factor(VEGF) and fibroblast growth factor 2(FGF2) are well-known growth factors involved in the regeneration of various tissues and organs, including peripheral nerve system. In the present study, we elucidated the local and systemic effects of plasmid construct рBud-coVEGF165-coFGF2 injected into the epineurium of intact rat sciatic nerve. Results of histological examination of sciatic nerve and multiplex immunoassays of serum showed the absence of immunogenicity and biosafety of plasmid рBud-coVEGF165-coFGF2. Moreover, local administration of plasmid DNA construct resulted in significantly decreased levels of pro-inflammatory cytokines in the peripheral blood, including tumor necrosis factor α(TNFα) and interleukin-12, and significantly increased levels of cytokines and chemokines including Regulated upon Activation, Normal T Cell Expressed and Presumably Secrete(RANTES), epidermal growth factor, interleukin-2, and monocyte chemoattractant protein 1. These changes in the peripheral blood on day 7 after injection of plasmid construct рBud-coVEGF165-coFGF2 show that the plasmid construct has systemic effects and may modulate immune response. At the same time, reverse transcriptionpolymerase chain reaction revealed transient expression of coFGF2, coVEGF165, ratFGF2 and ratVEGFA with direct transport of transcripts from distal part to proximal part of the sciatic nerve. Immunohistochemical staining revealed prolonged presence of VEGFA in sciatic nerve till 14 days post-injection. These findings suggest that local administration of plasmid construct рBud-coVEGF165-coFGF2 at a concentration of 30 ng/μl results in the formation of pro-angiogenic stimuli and, and the plasmid construct, used as a drug for gene therapy, might potentially facilitate regeneration of the sciatic nerve. The study was approved by the Animal Ethics Committee of Kazan Federal University, procedures were approved by the local Ethics Committee(approval No. 5) on May 27, 2014.

关键词： chemokines cytokines fibroblast growth factor gene therapy growth factors peripheral nerve system sciatic nerve regeneration vascular endothelial growth factor

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ID7 Isolated from Bauhinia variegata Stem Inhibits Tumor Progression and Metastatic Mechanisms of Triple Negative Breast Cancer in Vivo

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Journal of Pharmacy and Pharmacology 2019年第7期7卷 368-384页

作者： kamilla Monteiro dos Santos Izabela Natalia Faria Gomes Renato JoséSilva Oliveira Fernanda E.Pinto Bruno Oliveira Fabrício P.Batista Rafael Cesar Russo Chagas Hélio B.Santos Ralph G.Thomé Wanderson Romao Rui Manuel Reis Maria luiza Vilela Oliva Rosy Iara Maciel de Azambuja Ribeiro laboratory of Experimental Pathology Federal University of Sao Joao del-Rei(UFSJ)DivinópolisMGBrazil Josédo Rosário Vellano University UnifenasDivinópolisMGBrazil Molecular Oncology Research Center(CPOM) Barretos Cancer HospitalBarretosSPBrazil Petroleomic and Forensic Chemistry laboratory Department of ChemistryFederal Institute of Espírito Santo(IFES)VitóriaESBrazil Chemistry Department Federal University of Sao PauloBrazil Tissue Processing laboratory Federal University of Sao Joao del-Rei(UFSJ)DivinópolisMGBrazil life and Health Sciences Research Institute(ICVS) Medical SchoolUniversity of MinhoBragaPortugal 3ICVS/3B’s-PT Government Associate laboratory GuimaraesBragaPortugal

Background:The breast cancer has been the most common form of cancer among women.The triple negative subtype represents 20%of all breast cancer cases in the world and is standing out by affecting young women and being aggressive.The main cause of death of patients with cancer is due to metastasis,which can reach the liver and lungs.Objective:The activities of ID7 fraction of the stems of Bauhinia variegata l.on breast cancer,lung metastasis and liver inflammatory process were evaluated.Method:ID7 was characterized by mass-spectrometry.The viability of murine mammary cells(4T1)treated with ID7 was assessed by MTT,trypan blue and fluorescence assay and viability of BT-20,MDA-MB-231 and MCF-7 human breast cancer tumor lines by MTS.The cell migration,invasion using matrigel and adhesion were performed.The expression of cell death proteins was quanitified by western blot and the gelatinases by zimogram.The ID7 activity of the tumor(4T1)and metastatic progession in vivo was evaluated.Results:ID7 reduced the 4T1 and MDA-MB-231viability and increased the late apoptosis,inhibited the 4T1 migration and invasion,increased the 4T1 adhesion and decreased the secreted active gelatinases.ID7 also increased the expression of PARP,caspase-7 and caspase-8,RIP and TNF-R1.In vivo,the ID7 decreased the volume and weight of the tumors and decreased lung metastasis and inflammation in the liver.The characterization showed mainly the presence of oleic acid,myricetin,quercetin and kaempferol in ID7.Conclusion:Thus,it was found that ID7 fraction exhibits selective antitumor and on the mechanisms of breast cancer metastasis activity,preventing lung metastasis and inflammation in the liver.It is suggested that fatty acids and flavonoids are correlated with such activities.

关键词： 4T1 cells MDA-MB-231 medicinal plants kaempferol gelatinases apoptosis cell viabilty in vivo

糖尿病患者血浆纤维蛋白原及D二聚体水平变化及意义

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血栓与止血学 2013年第1期19卷 30-31页

作者：赵进卡米拉新疆自治区人民医院特检科新疆自治区人民医院检验科乌鲁木齐830001

目的探讨糖尿病患者血浆D二聚体、纤维蛋白原的改变及其临床意义。方法采用CA7000全自动血凝分析仪,检测46例糖尿病患者和健康对照组22例的血浆D二聚体、纤维蛋白原浓度。根据血糖浓度把糖尿病分为血糖(BS)≤10 mmol/l(26例)和BS≥10 mm... 详细信息

目的探讨糖尿病患者血浆D二聚体、纤维蛋白原的改变及其临床意义。方法采用CA7000全自动血凝分析仪,检测46例糖尿病患者和健康对照组22例的血浆D二聚体、纤维蛋白原浓度。根据血糖浓度把糖尿病分为血糖(BS)≤10 mmol/l(26例)和BS≥10 mmol/l(18例)两组,结果 D二聚体、纤维蛋白原含量明显高于健康对照组,而且随血糖浓度增高而升高,差异均有统计学意义(P<0.001)。结论血浆D二聚体、纤维蛋白原对糖尿病的早期诊断、预防和病情监测有一定程度的临床意义。

关键词：糖尿病 D二聚体纤维蛋白原

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老海勒鲁普高中体育馆

城市环境设计 2019年第2期118卷 114-127页

作者： Ana Merino Anders Hjortn?s Christian Alvarez Dennis Rasmussen Gül Ertekin Henrick Poulsen Hjalti Gestsson Jan Magasanik Jakob lange Jacob Thomsen Jeppe Ecklon Ji-young Yoon Michael Sch?nemann Narisara Schr?der Riccardo Mariano Rune Hansen Snorre Nash Thomas Juul-Jensen Vincent He Xu li Agnete Jukneviciute Ambra Chiesa Brigitta Gulyás Dennis Rasmussen Enea Michelesio Greta Krenciute H?gni laksáfoss Isabella Eriksson Jan Magasanik Mikkel Marcker Stubgaard Jeppe Ecklon kamilla Heskje Michael Sch?nemann Narisara Schr?der Thomas Juul-Jensen Brigitta Gulyás Enea Michelesio Jakob lange Mikkel Marcker Stubgaard Narisara Schr?der Tobias Hjortdal Iwan Baan Peter Moldow Rasmus Hjortshoj Jens lindhe 朱翰林

位于哥本哈根北部的老海勒鲁普高中,内部拥有1100m2的多功能大厅和最新的两层建筑,为现有学生提供了广阔的社交和创意展示空间,同时提升学校的容量,以应对持续增长的人口需求。原本校园旁边的一栋建筑里,体育设施匮乏而无法满足需求;老... 详细信息

位于哥本哈根北部的老海勒鲁普高中,内部拥有1100m2的多功能大厅和最新的两层建筑,为现有学生提供了广阔的社交和创意展示空间,同时提升学校的容量,以应对持续增长的人口需求。原本校园旁边的一栋建筑里,体育设施匮乏而无法满足需求;老海勒鲁普高中也缺少大型多功能空间,用以举办体育活动、毕业典礼和社交聚会。为此,BIG为学生

关键词：体育馆

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阿梅格尔巴克/哥本山

城市环境设计 2019年第2期 172-183页

作者： Nanna Gyldholm M?ller Alberto Cumerlato Aleksander Wadas Alexander Codda Alexander Ejsing Alexandra Gustafsson Alina Tamosiunaite Armor Gutierrez Anders Hjortn?s Andreas Klok Pedersen Annette Jensen Ariel Wallner Ask Andersen Balaj IIulian Blake Smith Borko Nikolic Brygida Zawadzka Buster Christensen Chris Falla Chris Zhongtian Yuan Daniel Selensky Dennis Rasmussen Espen Vik Finn N?rkj?r Franck Fdida Gonzalo Castro Gül Ertekin George Abraham Helen Chen Henrick Poulsen Henrik R?mer Kania Horia Spirescu Jakob Ohm laursen Jelena Vucic Jeppe Ecklon Ji-young Yoon Jing Xu Joanna Jakubowska Johanna Nenander kamilla Heskje Katarzyna Siedlecka Krzysztof Marciszewski laura W?tte liang Wang lise Jessen long Zuo Maciej Zawadzki Mads Enggaard Stidsen Marcelina Kolasinska Marcos Bano Maren Allen Mathias Bank Matti N?rgaard Michael Andersen Narisara ladawal Schr?der Niklas A. Rasch Nynne Madsen ?ssur Nols? Pero Vukovic Richard Howis Ryohei Koike Se Hyeon Kim Simon Masson Sunming lee Toni Mateu Xing Xiong Zoltan David Kalaszi Rasmus Hjortshoj 陈子钰

垃圾焚烧发电厂阿梅格尔巴克/哥本山位于工业区,多年来,该工业区已成为冒险者们的极限运动目的地。原始的工业设施为各种极限运动活动提供了场所,如缆绳滑水、卡丁车和攀岩等。阿梅格尔巴克/哥本山是该地区最重要的地标,其建筑亟须更新... 详细信息

垃圾焚烧发电厂阿梅格尔巴克/哥本山位于工业区,多年来,该工业区已成为冒险者们的极限运动目的地。原始的工业设施为各种极限运动活动提供了场所,如缆绳滑水、卡丁车和攀岩等。阿梅格尔巴克/哥本山是该地区最重要的地标,其建筑亟须更新。我们提出了一种新型的垃圾焚烧发电厂,这种工厂能产生经济、环保和社会效益。我们不将阿梅格尔巴克/哥本山视为一个孤立的对象,而是调动起建筑的

关键词：巴克本山