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Evaluation of anticancer effects of a pharmaceutically viable extract of a traditional polyherbal mixture against non-small-cell lung cancer cells

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Journal of Integrative Medicine 2020年第3期18卷 242-252页

作者： Varuni Colamba Pathiranage Ira Thabrew Sameera R.Samarakoon kamani h.tennekoon Umapriyatharshini Rajagopalan Meran K.Ediriweera Institute of Biochemistry Molecular Biology and BiotechnologyCumaratunga Munidasa MawathaUniversity of ColomboColombo 03Sri Lanka

Objective: The present work tested organic solvents to prepare an extract with anticancer properties from a polyherbal mixture containing Nigella sativa(seeds), hemidesmus indicus(roots) and Smilax glabra(rhizomes). We evaluate anticancer effects in non-small-cell lung cancer cells(NCI-h292), and discuss optimization for pharmaceutical use in the context of efficacy, yield and ***: Using different organic solvents, six extracts were prepared from the polyherbal mixture. Based on the cytotoxic effects of these extracts on NCI-h292 cells and normal lung cells(MRC-5), as evaluated by the sulphorhodamine B assay, the total ethyl acetate(T-EA) extract was selected for further *** possible anticancer mechanisms were assessed by evaluating the extract’s effects on apoptosis(through fluorescent microscopic analysis, DNA fragmentation analysis, caspase 3/7 assay and analysis of expression levels of apoptosis-related genes p53, Bax, survivin, hsp70 and hsp90), colony formation and antioxidant ***: The extract had cytotoxic effects against NCI-h292 cells in a time-and dose-dependent *** antioxidant activity and inhibition of colony formation were also observed. The expression level of caspase 3/7 significantly(P h292 cells treated with 50 lg/m L of the extract. The same dosage led to a significant increase in expression levels of Bax and p53(P hsp70 and ***: T-EA extract of the above polyherbal mixture has cytotoxicity against NCI-h292 cells via induction of apoptosis, antioxidant effects and inhibition of colony formation.

关键词： Nigella sativa hemidesmus indicus Smilax glabra Lung cancer Apoptosis Antioxidant activity

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Isolation and Characterization of R-Enantiomer in Ezetimibe

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American Journal of Analytical Chemistry 2013年第9期4卷 488-495页

作者： Kameswararao Chimalakonda Venugopal kamani Madhusudhan Gutta Srinivasulu Polisetty Sai Venkata Srinivas Koduri Inogent laboratories Pvt Ltd. IDA-Nacharam Jawaharlal Nehru Technological University Hyderabad Hyderabad India

A simple and rapid Supercritical Fluid Chromatography (SFC) method has been developed to isolate and characterize R-Isomer of Ezetimi be by using normal phase Chiral Cel OD-h with 250 mm × 30 mm, 5 microns column using a mobile phase system containing super critical fluid carbondi oxide (Co2) and the percentage of 2-Propanol as a mobile phase (85:15) and detection at 230 nm. The isolated R-Isomer is characterized by using UV-vis, FT-IR, ESI-MS, hPLC1h and 13C NMR. The purity of isolated R-Isomer is about 98%.

关键词： Isolation Characterization (R)-Isomer Ezetimibe Supercritical Fluid hromatography (SFC)

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