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Effect of age on reproductive attributes of an aphidophagous ladybird, Cheilomenes sexmaculata

Insect Science 2006年第4期13卷 301-308页

作者： OMKAR SATYENDRA K. singh kalpana singh Ladybird Research Laboratory Department of Zoology University of Lucknow Lucknow India

The effect of both male and female age was investigated on certain reproductive attributes, viz. mating incidence, mating duration, fecundity, percent egg viability, ratio of reproductive and non-reproductive periods and reproductive rate, of an aphidophagous ladybird, Cheilomenes sexmaculata （Fabricius）. Females started mating at the age of 8 hours post-emergence （PE） and males at the age of 2 days PE. Mating in the laboratory was a maledominated phenomenon. The mating duration and reproductive rate of 10-day-old females when mated with males of varying ages increased up to the male age of 60 days, and thereafter decreased, whereas, fecundity, egg viability and ratio of reproductive and non-reproductive periods increased up to the male age of 50 days, and thereafter declined. However, when females of varying ages were mated with 10-day-old males, fecundity and reproductive rate increased up to 40 days of female age, respectively, then decreased. The ratio of reproductive and non-reproductive periods increased with increasing age of females. Mating age for optimal reproductive output was 10-50-day-old males and NE to 40-day-old females. Reproductive cessation in males was recorded after 50 days PE, whereas in females at the age of 40 days PE. Higher mating durations lead to elevated reproductive rates. Delay in the reproductive phase was positively correlated with longevity. The results of this study may aid mass multiplication of this ladybird by identifying and promoting usage of adults of optimal age, Our results also enhance our understanding of the effect of age on reproductive attributes in ladybirds.

关键词： age Cheilomenes sexmaculata egg viability fecundity ladybird longevity,protogyny reproduction reproductive rate

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A novel D(−)-lactic acid-inducible promoter regulated by the GntR-family protein D-LldR of Pseudomonas fluorescens

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Synthetic and Systems Biotechnology 2019年第3期4卷 157-164页

作者： kalpana singh Satish Kumar Ainala Yeonhee Kim Sunghoon Park School of Energy and Chemical Engineering UNISTUlsan44919Republic of Korea School of Chemical and Biomolecular Engineering Pusan National UniversityBusan46241Republic of Korea

Lactic acid has two stereoisomers of D(−)-and L(+)-forms,both of which are important monomers of biodegradable plastic,poly-lactic *** this study,a novel D-lactate inducible system was identified in Pseudomonas fluorescens A506,partially characterized and tested as *** D-lactate catabolic operon(lldP-dld-II)was negatively regulated through the inversely transcribed D-lldR(encoding a GntR-type regulator),where the repression is relieved by addition of *** derepression was specific to D-lactate and marginally affected by *** D-LldR-responsive operator,showing dyad symmetry and separated by one base,was located between+11 and+27 from the transcription start site of the lldP-dld-II *** site-directed mutagenesis,a motif with a dyad symmetry(AATTGGTAtTACCAATT),present in the upstream region of lldP,was identified as essential for the binding of LldR.D-lactate biosensors were developed by connecting the upregulation by D-lactate to a green fluorescent ***^6.0-fold induction by 100mM D-lactate was observed compared to Llactate.

关键词： Lactic acid Enantiomer Biosensor Repression

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Survival, development and life tables of two congeneric ladybirds in aphidophagous guilds

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Insect Science 2006年第2期13卷 119-126页

作者： AVANISH K. GUPTA SHEFALI SRIVASTAVA GEETANJALI MISHRA kalpana singh OMKAR Ladybird Research Laboratory Department of Zoology University of Lucknow Lucknow India

Two congeneric aphidophagous ladybirds, Coccinella septempunctata and Coccinella transversalis, were reared on three aphid species, Lipaphis erysimi, Myzus persicae and Aphis nerii, to estimate the effect of prey quality and intra- and interspecific interactions on their survival and development of life stages. Mortality of first instar ladybirds of both species was highest feeding on A. nerii. Preimaginal mortafity was lowest when feeding on L. erysimi （C. septempunctata, 1.6% and C. transversalis, 3.2%）, and highest when feeding on A. nerii （ C. septempunctata, 6.2% and C. transversalis, 8.2%）. Comparatively higher weight and larger size of C. septempunctata along with the lower levels of mortality recorded suggested that it is more likely to have acted as an intraguild predator than C. transversalis. High recorded mortality of C. transversalis is attributed to probable intraguild predation on account of its smaller size. The major sources of mortality were probably cannibalism, intraguild predation and other unknown factors. Lower prey quality increased the incidence of cannibalism and intraguild predation, especially in C. transversalis. The investigation suggests an intrinsic competitive advantage for C. septempunctata over C. transversalis in guilds of three aphid species.

关键词： Coccinella septempunctata Coccinella transversalis ladybirds life table,aphids cannibalism intraguild predation

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Association of NFKB1 gene polymorphism (rs28362491) with levels of inflammatory biomarkers and susceptibility to diabetic nephropathy in Asian Indians

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World Journal of Diabetes 2017年第2期8卷 66-73页

作者： Amar Gautam Stuti Gupta Mohit Mehndiratta Mohini Sharma kalpana singh Om P Kalra Sunil Agarwal Jasvinder K Gambhir Department of Medicine University College of Medical Sciences (University of Delhi) and G.T.B. Hospital Molecular Diagnostic Laboratory Department of Biochemistry University College of Medical Sciences (University of Delhi) and G.T.B. Hospital

AIM To investigate the association of NFKB1 gene-94 ATTG insertion/deletion(rs28362491) polymorphism with inflammatory markers and risk of diabetic nephropathy in Asian *** A total of 300 subjects were recruited(100 each), normoglycemic,(NG); type 2 diabetes mellitus(T2DM) without any complications(DM) and T2 DM with diabetic nephropathy [DM-chronic renal disease(CRD)]. Analysis was carried out by polymerase chain reaction-restriction fragment length polymorphism and ELISA. Pearson's correlation, analysis of variance and logistic regression wereused for statistical *** The allelic frequencies of-94 ATTG insertion/deletion were 0.655/0.345(NG), 0.62/0.38(DM) and 0.775/0.225(DM-CRD). The-94 ATTG ins allele was associated with significantly increased levels of urinary monocyte chemoattractant protein-1(u MCP-1); u MCP-1(P = 0.026) and plasma tumor necrosis factor-alpha(TNF-α); TNF-α(P = 0.030) and almost doubled the risk of diabetic nephropathy(OR = 1.91, 95%CI: 1.080-3.386, P = 0.025).CONCLUSION-94 ATTG ins/ins polymorphism might be associated with increased risk of developing nephropathy in Asian Indian subjects with diabetes mellitus.

关键词： Diabetic nephropathy Inflammation NFKB1 -94 ATTG ins/del polymorphism Urinary monocyte chemoattractant protein-1 Tumor necrosis factor-alpha

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Evidence-Based Practice among Critical Care Nurse’s/Midwives in Qatar

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Open Journal of Nursing 2022年第1期12卷 42-59页

作者： Badriya Al-Lenjawi Jibin Kunjavara Nesiya Hassan Kamaruddeen Mannethodi Ederlie Martinez George V. Joy kalpana singh Department of Nursing and Midwifery Research Hamad Medical Corporation Doha Doha Qatar

Background: Successful implementation of evidence-based research into clinical practice was determined by four core elements, self-belief, the nature and level of evidence, the conductive context into which the study is to be implemented, and organizational factors facilitating the process. Aim: The current study aimed to examine barriers influencing evidence-based practice among critical care nurses in QATAR. Methods: A cross-sectional survey was utilized for the study resulting in data collected from 278 nurses during the period of Feb-2021 to March 2021 using the Barriers to Research Utilization Scale (BTRUS). The BTRUS consisted of data on various information sources utilized by nurses for support in practice, potential barriers for evidence-based practice, and perceived skills on applying research-based evidence. Result: The most significant organizational barriers were lack of time, lack of empowerment to change practice, lack of support from colleagues in implementing research evidence, and lack of access to research articles. Self-perceived barriers were irrelevance of research evidence to current practice, studies having methodological flaws, skepticism about research findings, a large amount of research evidence, and inability to understand statistics. The Nurse’s age, years of nursing practice, academic attainment, and organizational position influenced self-reported barriers and utilization of sources of Evidence. Conclusion: Organizational support, improved self-belief, and evidence-based practice expertise may reduce barriers to implementing research evidence in clinical Practice.

关键词： EBP—Evidence Based Practice CNS/CMS—Clinical Nurse/Midwife Specialists

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Three-phase AC-DC Converter for Direct-drive PMSG-based Wind Energy Conversion System

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Journal of Modern Power Systems and Clean Energy 2023年第2期11卷 589-598页

作者： Kumar Abhishek singh Ayushi Chaudhary kalpana Chaudhary Department of Electrical Engineering Indian Institute TechnologyVaranasiIndia Department of Electrical Engineering Indian Institute TechnologyKanpurIndia

In this paper,a wind energy conversion system(WECS)is presented for the electrification of rural areas with wind energy availability.A three-phase AC-DC converter based on a bridgeless Cuk converter is used for power extraction from the permanent magnet synchronous generator(PMSG).The bridgeless topology enables the elimination of the front-end diode bridge rectifier(DBR).Moreover,the converter has fewer components,simple control,and high efficiency,making it suitable for a small-scale WECS.A squirrel cage induction motor(SCIM)is used to emulate a MOD-2 wind turbine to implement the PMSG-based WECS.A direct-drive eight-pole PMSG is used in this study;thus,a low-input-voltage system is *** converter is designed to operate in the discontinuous inductor current mode(DICM)for inherent power factor correction(PFC)and the maximum power point tracking(MPPT)is achieved through the tip-speed ratio(TSR)*** performance of the developed system is analyzed through simulation,and a 500 W hardware prototype is developed and tested in different wind speed conditions.

关键词： Permanent magnet synchronous generator(PMSG) three-phase AC-DC converter wind energy conversion system(WECS) wind turbine emulator

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Spontaneous rupture of intrahepatic pseudocyst into the inferior vena cava

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Gastroenterology Report 2018年第3期6卷 225-227,I0003页

作者： Yashwant Patidar Binit Sureka Vaibhav Pratap singh kalpana Bansal Rakhi Maiwall Department of Radiology/Interventional Radiology Institute of Liver and Biliary SciencesNew DelhiIndia

We present an extremely rare case of caudate lobe intrahepatic pancreatic pseudocyst with spontaneous rupture into the inferior vena cava(IVC).A 58-year-old male,a chronic alcoholic,presented with features of pancreatitis for which imaging was *** and contrast-enhanced computed tomography were carried out,which revealed intrahepatic pancreatic pseudocyst in the caudate lobe of the *** was suggestion of spontaneous rupture of the pseudocyst into the IVC,which was well delineated on imaging.

关键词： pancreatic pseudocyst spontaneous rupture inferior vena cava

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Considering the Fault Dependency Concept with Debugging Time Lag in Software Reliability Growth Modeling Using a Power Function of Testing Time

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International Journal of Automation and computing 2007年第4期4卷 359-368页

作者： V.B.singh kalpana Yadav Reecha Kapur V.S.S.Yadavalli Delhi College of Arts and Commerce University of DelhiDelhi 110023India Indira Gandhi Institute of Technology Guru Gobind Singh Indraprastha UniversityDelhi 110006India Department of Mathematics and Computer Application Bundelkhand UniversityJhansi 284128India Department of Industrial and Systems Engineering University of PretoriaPretoria 0002South Africa

Since the early 1970s tremendous growth has been seen in the research of software reliability growth modeling. In general, software reliability growth models （SRGMs） are applicable to the late stages of testing in software development and they can provide useful information about how to improve the reliability of software products. A number of SRGMs have been proposed in the literature to represent time-dependent fault identification / removal phenomenon; still new models are being proposed that could fit a greater number of reliability growth curves. Often, it is assumed that detected faults are immediately corrected when mathematical models are developed. This assumption may not be realistic in practice because the time to remove a detected fault depends on the complexity of the fault, the skill and experience of the personnel, the size of the debugging team, the technique, and so on. Thus, the detected fault need not be immediately removed, and it may lag the fault detection process by a delay effect factor. In this paper, we first review how different software reliability growth models have been developed, where fault detection process is dependent not only on the number of residual fault content but also on the testing time, and see how these models can be reinterpreted as the delayed fault detection model by using a delay effect factor. Based on the power function of the testing time concept, we propose four new SRGMs that assume the presence of two types of faults in the software： leading and dependent faults. Leading faults are those that can be removed upon a failure being observed. However, dependent faults are masked by leading faults and can only be removed after the corresponding leading fault has been removed with a debugging time lag. These models have been tested on real software error data to show its goodness of fit, predictive validity and applicability.

关键词： Non-homogeneous Poisson process fault dependency leading fault dependent fault delay effect.

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氨化碳纳米管接枝生物分子(蛋白质和DNA)(英文)

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新型炭材料 2009年第4期24卷 301-306页

作者： kalpana Awasthi D.P. singh Sunil singh D. Dash O.N. Srivastava Department of Physics Banaras Hindu University Varanasi-221005 INDIA Department of Physics Southern Illinois University Carbondale Lincoln Carbondale-629014401 USA Department of Biochemistry Institute of Medical Sciences Banaras Hindu University Varanasi-221005 INDIA

报告-种氨基功能化处理的多壁碳纳米管(f-MWCNTs)用于生物分子〔如牛血浆蛋白素(BSA)蛋白质及脱氧核醣核酸(DNA)〕的接枝处理高效方法。以苯—二茂铁喷入氩氛炉于～850℃裂解制得MWCNTs,,而后向纳米管表面导入羧基化合物,再以氨基基团对... 详细信息

报告-种氨基功能化处理的多壁碳纳米管(f-MWCNTs)用于生物分子〔如牛血浆蛋白素(BSA)蛋白质及脱氧核醣核酸(DNA)〕的接枝处理高效方法。以苯—二茂铁喷入氩氛炉于～850℃裂解制得MWCNTs,,而后向纳米管表面导入羧基化合物,再以氨基基团对MWCNTs,进行功能化处理。该过程包括:乙二胺与羧基基团直接结合,经酰胺化引入胺基基团。对制得的MWCNTs、f-MWCNTs以及由BSA蛋白质和DNA接枝的氨化f-MWCNTs等样品,采用SEM、TEM、FTIR进行表征。结果表明:生物分子(BSA蛋白质和DNA)接枝于氨化的f-MWCNTs。

关键词：碳纳米管功能化处理蛋白质 DNA

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Expression of TTV-ORF2 Protein for Detection of Anti-TTV IgG Antibodies in Human Sera

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Advances in Infectious Diseases 2013年第3期3卷 223-229页

作者： Shiwani singh Akanksha singh Dhananjay singh Mankotia kalpana Luthra Mohammad Irshad Clinical Biochemistry Division Department of Laboratory Medicine All India Institute of Medical Sciences New Delhi India

The present study describes the cloning and expression of ORF-2 region of TTV genome and the use of expressed peptide in developing immunoassay for detection of anti-TTV antibodies in serum. Presence of TTV-DNA in serum was detected by PCR amplifying N-22 region of ORF-1 of TTV genome. This was followed by genotyping of TTV by RFLP using N-22 amplicon. Using genotype-1 positive serum as the source of TTV, the ORF-2 region was amplified by PCR and subsequently cloned and expressed in pET-19b vector. The expressed protein, identified as 20 kDa protein on SDS-PAGE gel, was purified by affinity chromatography and then used as antigen to develop western blot assay for detection of anti-TTV antibodies in serum. Analysis of sera for anti-TTV antibodies and their comparison with presence of TTV-DNA, produced encouraging results. There was a good relation between presence of anti-TTV and TTV-DNA in these sera samples. Anti-TTV antibodies could be detected in all TTV-DNA positive sera irrespective of the presence of TTV-genotype. This investigation demonstrates that ORF-2 peptide may be used in developing immunoassay for identification of TTV infection.

关键词： TTV ORF-2 Anti TTV-IgG Expression N-22

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